Sri Estuningsih
epartemen Klinik Reproduksi dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor

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Deteksi Dini Penyakit Tumor Sel Darah Myelosit Leukosis melalui Pemeriksaan Ulas Darah Agungpriyono, Dewi Ratih; Huminto, Hernomoadi; Estuningsih, Sri; Satyaningtijas, Aryani Sismin
Jurnal Ilmu Pertanian Indonesia Vol 12, No 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Myelocytes blood cell tumor in chicken is a disease caused by retrovirus, avian leukosis virus subgroup J (ALV-J). The virus has the same group as human retrovirus (HIV) which caused AIDS, but the avian type possesses oncogenic properties, that could induce cell transformation and tumor formation. ALV-J stimulates the bone marrows myelocytes and transforms them into tumor cell myelocytoma. The tumor cells then metastasis through the circulatory system causing myeloid leukosis and tumor cells accumulation in various internal organs or myelocytomatosis. This study was done on the base of the leukosis behavior of the tumor. The finding of the metastasis tumor cell in the blood smear examination is thought could be use as the diagnostic clue of the disease. Blood smear from sick chickens are collected and stained with some chemical staining substance such as may grunwald-giemsa, hematoxyllin eosin, periodic acid Schiff, congo red, toluidine blue, and sudan black B. Cytochemistry character of the blood cells was observed using light microscope. The result showed that myelocytes granules were best observed using hematoxyllin eosin, periodic acid Schiff, congo red and toluidine blue while may grunwald-giemsa, and sudan black B could not differentiate the granules. By this method, the field veterinarian will able to screen the suspected chicken flock for myeloid leukosis earlier than the occurrence of tumor formation.
APLIKASI MUTAN BERFLUORESENS UNTUK MEMPELAJARI KETAHANAN HIDUP, KOLONISASI DAN PENETRASI ISOLAT Cronobacter sakazakii SELAMA PENGERINGAN JAGUNG [Use of GFP Mutant to Study the Survival, Colonization and Penetration of Cronobacter sakazakii Isolates Duri Nurjanah, Siti; Suhartono, Maggy T.; Hariyadi, Ratih Dewanti-; Estuningsih, Sri
Jurnal Teknologi Dan Industri Pangan Vol 24, No 2 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Cronobacter sakazakii is a Gram-negative emerging pathogen regarded as causative agent of meningitis and necrotizing enterocolitis in certain groups of infants. In the previous research, thirty-two local isolates of C. sakazakii were obtained from various dried food products such as from corn starch, suggesting that they are able to survive drying. Some of the isolates were toxic. Green Fluorescent Protein (GFP) have been inserted to C. sakazakii and used as a marker for selective enumeration due to the ability of this protein to fluoresce under UV and to tolerate in ampicillin containing media. The objective of this study was to evaluate the survival, colonization and penetration of two isolates of C. sakazakii from dried food product during maize drying. The maize was challenged with mutants at a concentration of 105-106 CFU/g before drying. Maize drying was performed at temperature of 40ºC, 45ºC and 50ºC for 4, 6 and 8 days until the moisture content reached 14%. The totals of resistant drying mutants were counted every day onto ampicillin containing media by observing under UV light. The survival rate of C. sakazakii during drying was determined by the slope of linier regression from C. sakazakii survival curve. Isolates of FWHd16, the toxic strain of C. sakazakii, were more resistant to heat treatments in comparison to isolates of YRt2a, or the non toxic strain of C. sakazakii. Following fluorescence and scanning electron microscope observation, it is concluded that both isolates were colonizing on maize surface. These mutants were able to penetrate to the inner side of the grain by entering injured surface or pores at the tip cap of maize.
Effect of clove cigarette exposure on white rat : special emphasis on the histopathology of respiratory tract Widodo, Eddy; Priosoeryanto, Bambang P.; Estuningsih, Sri; Agungpriyono, Dewi R.; Utji, Robert
Medical Journal of Indonesia Vol 16, No 4 (2007): October-December
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Cigarette smoke is proved to cause various disturbances on respiratory tract. Clove cigarette is far more dangerous than common (“white”) cigarette, since the tar, nicotine, and carbon monoxyde content is significantly higher. In Indonesia, 88% smokers consume clove cigarette. The clove cigarette effect to the respiratory tract have never been studied. Aim of this  research is to study histopathological changes of respiratory tract in Sprague-Dawley white rats after smoke cigarette exposure. The study was performed using 20 white rats starting September 2005 until May 2006. Necropsy was done after final day of smoke exposure, then histopathological slides of the respiratory tract were processed and stained under light microscope and videomicrometer. Observed parameters were height and number of ciliated epithelia and goblet cells, also number of pneumocytes types I, II, and macrophages, and interstitial lung tissue reactions. The latest parameters were observed with semi-thin sections of resin embedded lung stained with Toluidine Blue. Result showed considerable histopathological changes on respiratory tract. The amount of epithelial cells on the group exposed to clove cigarette smoke were significantly higher than control group (P<0.05) on sinus, bronchi, and bronchioli area, while no significant difference were found on trachea (P>0.05). Number of goblet cells in exposed group was also higher (P>0.05). The epithelial height in exposed group was higher compared to control, but no statistical differences were found between male and female rats. The interstitial  pneumonia score was statistically different (P<0.05) between the two groups. The amount of pneumocytes type II was higher than types I within the exposure group. Based on all mentioned above, we suggest that clove cigarette smoke exposure causes pathological disorders in rat respiratory tract. (Med J Indones 2007; 16:212-8)Keywords: clove cigarette, respiratory tract histopathology, respiratory tract toxicopathology.
Stabilitas Mikrob Usus, Histologi Hati dan Ginjal Mencit Setelah Pemberian Ekstrak Pliek u Bumbu Masak Tradisional Aceh (STABILITY OF GUT MICROBIAL AND HISTOLOGY OF LIVER AND KIDNEY OF MICE AFTER ADMINISTRATION OF PLIEK-U EXTRACT ., Nurliana; Estuningsih, Sri; ., Sugito; Masyitha, Dian
Jurnal Veteriner Vol 15, No 3 (2014)
Publisher : Jurnal Veteriner

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Abstract

Pliek u is one of the traditional spices of Aceh prepared by fermentation of coconut meat which is usedas chicken feed additive too. The stability of gut micro flora and histopathological changes of liver andkidney of mice were detected after three days oral administration of acute single dose of ethanol extract ofpliek u. Nine animals were used and grouped into three; in which group I and II (treatment groups) wereadministered 370 and 733 mg/kg body weight of pliek u, respectively, and group III referred as the control.At the fourth day of experiment, all animals were sacrificed, and their livers, kidneys and intestinal gutwere excised. The gut microbial was measured by Total Plate Count (TPC). Livers and kidneys wereprocessed for paraffin procedure and hematoxyllin-eosin staining. The results showed that the ethanolextract of pliek u has no significant effect (P>0.05) on the TPC of the gut microbial and the structure of theliver and kidney of mice. It can be concluded that administration of the two doses (370 and 730 mg/kg bw)of pliek u did not lowering the numbers of gut microbial and were not toxic to the livers and kidneys of themice.
Dyspepsia in Nonsteroidal Anti-inflammatory Drugs Gastropathy Manan, Chudahman; Priosoeryanto, Bambang Pontjo; Daldiyono, Daldiyono; Estuningsih, Sri; Rahminiwati, Min
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 2, August 2011
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) gastropathy is a common complication, which has characteristic symptoms of dyspepsia syndrome. Mostly, it includes epigastric discomfort with bloating and nausea. The aim of this study was to provide evidences that clinical symptoms of dyspepsia are related to macroscopic changes of gastric form in rats, which are expected to be applied in human. Method: The study was conducted in 20 white rats (Rattus norvegicus, Sprague-Dawley strain) at the Department of Pathology and Clinical Reproduction, Bogor Agricultural University between January and December 2008. The rats were divided to treatment group and control group and each group consisted of 10 rats. Acetyl salicylic acid (ASA/aspirin) was administered at 400 mg dose, diluted in distilled water and was given to the treatment group using gastric cannula, once daily for three days period; while the control group had received aquabidest only. Subsequently, necropsies were conducted for both groups, followed by macroscopic observation and measurement of sagittal and transversal diameter. Gastric incisions along the minor curvature were performed in both groups to recognize any macroscopic changes of gastric mucosa. ANOVA test was utilized for data analysis, which was followed by Duncan test when the results were significant. Results: Gastric diameters in treatment group with positive lesion were significantly different from the control group and the treatment group with negative lesion on anthrum/pylorus region, with p < 0.05. Conclusion: Prominent gastric dilatation at anthrum/pylorus region found in the treatment group may become the initial cause and signs of dyspepsia in human. Keywords: NSAID gastropathy, dyspepsia, NSAIDs/aspirin, gastric dilatation
Preventing NSAID-induced Gastropathy: The Role of Mucus Cells to Prevent Aspirin-Induced Acute Gastric Mucosal Damage Manan, Chudahman; Priosoeryanto, Bambang Pontjo; Daldiyono, Daldiyono; Estuningsih, Sri; Rahminiwati, Min
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 3, December 2011
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Mucus is pre-epithelial gastric layer that may prevent damages due to direct contact between aspirin and gastric epithelial cells. The integrity of gastric mucosa and mucous cellular reaction may serve as primary and secondary prevention of extended aspirin-induced gastric mucosal damage. The aim of this study was to prove the function of mucus as defensive factor in rats. Method: The study was conducted in twenty white rats of the Sprague-Dawley strain at Department of Pathology and Clinical Reproduction, Bogor Agricultural University, between January and December 2008. The rat in the treatment group were given 400 mg aspirin diluted in aqua bidest through intra- gatric canules; while the control group received aqua bidest only once daily for 3 days. Necropsies, macroscopic and microscopic observation were performed by counting the number of Alcian blue- periodic acid Schiff-stained mucous cells at fundus/corpus and antrum/pylorus regions. Data analysis was performed using ANOVA and Duncan test. Results: The number of mucous cells with positive lesions in the treatment group was significantly different from the control group at both regions. There was no significant difference of negative lesions between treatment and control group at both regions. At antrum/pylorus region, there was no difference of negative lesions between treatment and control groups; however, both groups demonstrated significant difference of positive lesions in treatment group. Conclusion: In primary prevention for gastric mucosal lesions, there is no increasing number of mucous cells in normal mucosa. Increasing number of mucous cells is a secondary prevention against extended aspirin-induced gastric mucosal damage. Keywords: NSAIDs/ASA, mucus cells, gastric mucosal lesion, rat
Deteksi Dini Penyakit Tumor Sel Darah Myelosit Leukosis melalui Pemeriksaan Ulas Darah Agungpriyono, Dewi Ratih; Huminto, Hernomoadi; Estuningsih, Sri; Satyaningtijas, Aryani Sismin
Jurnal Ilmu Pertanian Indonesia Vol 12, No 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Original Source | Check in Google Scholar | Full PDF (149.708 KB)

Abstract

Myelocytes blood cell tumor in chicken is a disease caused by retrovirus, avian leukosis virus subgroup J (ALV-J). The virus has the same group as human retrovirus (HIV) which caused AIDS, but the avian type possesses oncogenic properties, that could induce cell transformation and tumor formation. ALV-J stimulates the bone marrows myelocytes and transforms them into tumor cell myelocytoma. The tumor cells then metastasis through the circulatory system causing myeloid leukosis and tumor cells accumulation in various internal organs or myelocytomatosis. This study was done on the base of the leukosis behavior of the tumor. The finding of the metastasis tumor cell in the blood smear examination is thought could be use as the diagnostic clue of the disease. Blood smear from sick chickens are collected and stained with some chemical staining substance such as may grunwald-giemsa, hematoxyllin eosin, periodic acid Schiff, congo red, toluidine blue, and sudan black B. Cytochemistry character of the blood cells was observed using light microscope. The result showed that myelocytes granules were best observed using hematoxyllin eosin, periodic acid Schiff, congo red and toluidine blue while may grunwald-giemsa, and sudan black B could not differentiate the granules. By this method, the field veterinarian will able to screen the suspected chicken flock for myeloid leukosis earlier than the occurrence of tumor formation.
APLIKASI MUTAN BERFLUORESENS UNTUK MEMPELAJARI KETAHANAN HIDUP, KOLONISASI DAN PENETRASI ISOLAT Cronobacter sakazakii SELAMA PENGERINGAN JAGUNG [Use of GFP Mutant to Study the Survival, Colonization and Penetration of Cronobacter sakazakii Isolates Duri Nurjanah, Siti; Suhartono, Maggy T.; Hariyadi, Ratih Dewanti-; Estuningsih, Sri
Jurnal Teknologi dan Industri Pangan Vol 24, No 2 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Original Source | Check in Google Scholar | Full PDF (616.174 KB)

Abstract

Cronobacter sakazakii is a Gram-negative emerging pathogen regarded as causative agent of meningitis and necrotizing enterocolitis in certain groups of infants. In the previous research, thirty-two local isolates of C. sakazakii were obtained from various dried food products such as from corn starch, suggesting that they are able to survive drying. Some of the isolates were toxic. Green Fluorescent Protein (GFP) have been inserted to C. sakazakii and used as a marker for selective enumeration due to the ability of this protein to fluoresce under UV and to tolerate in ampicillin containing media. The objective of this study was to evaluate the survival, colonization and penetration of two isolates of C. sakazakii from dried food product during maize drying. The maize was challenged with mutants at a concentration of 105-106 CFU/g before drying. Maize drying was performed at temperature of 40ºC, 45ºC and 50ºC for 4, 6 and 8 days until the moisture content reached 14%. The totals of resistant drying mutants were counted every day onto ampicillin containing media by observing under UV light. The survival rate of C. sakazakii during drying was determined by the slope of linier regression from C. sakazakii survival curve. Isolates of FWHd16, the toxic strain of C. sakazakii, were more resistant to heat treatments in comparison to isolates of YRt2a, or the non toxic strain of C. sakazakii. Following fluorescence and scanning electron microscope observation, it is concluded that both isolates were colonizing on maize surface. These mutants were able to penetrate to the inner side of the grain by entering injured surface or pores at the tip cap of maize.
Dyspepsia in Nonsteroidal Anti-inflammatory Drugs Gastropathy Manan, Chudahman; Priosoeryanto, Bambang Pontjo; Daldiyono, Daldiyono; Estuningsih, Sri; Rahminiwati, Min
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 2, August 2011
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) gastropathy is a common complication, which has characteristic symptoms of dyspepsia syndrome. Mostly, it includes epigastric discomfort with bloating and nausea. The aim of this study was to provide evidences that clinical symptoms of dyspepsia are related to macroscopic changes of gastric form in rats, which are expected to be applied in human. Method: The study was conducted in 20 white rats (Rattus norvegicus, Sprague-Dawley strain) at the Department of Pathology and Clinical Reproduction, Bogor Agricultural University between January and December 2008. The rats were divided to treatment group and control group and each group consisted of 10 rats. Acetyl salicylic acid (ASA/aspirin) was administered at 400 mg dose, diluted in distilled water and was given to the treatment group using gastric cannula, once daily for three days period; while the control group had received aquabidest only. Subsequently, necropsies were conducted for both groups, followed by macroscopic observation and measurement of sagittal and transversal diameter. Gastric incisions along the minor curvature were performed in both groups to recognize any macroscopic changes of gastric mucosa. ANOVA test was utilized for data analysis, which was followed by Duncan test when the results were significant. Results: Gastric diameters in treatment group with positive lesion were significantly different from the control group and the treatment group with negative lesion on anthrum/pylorus region, with p < 0.05. Conclusion: Prominent gastric dilatation at anthrum/pylorus region found in the treatment group may become the initial cause and signs of dyspepsia in human. Keywords: NSAID gastropathy, dyspepsia, NSAIDs/aspirin, gastric dilatation
Preventing NSAID-induced Gastropathy: The Role of Mucus Cells to Prevent Aspirin-Induced Acute Gastric Mucosal Damage Manan, Chudahman; Priosoeryanto, Bambang Pontjo; Daldiyono, Daldiyono; Estuningsih, Sri; Rahminiwati, Min
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 3, December 2011
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Background: Mucus is pre-epithelial gastric layer that may prevent damages due to direct contact between aspirin and gastric epithelial cells. The integrity of gastric mucosa and mucous cellular reaction may serve as primary and secondary prevention of extended aspirin-induced gastric mucosal damage. The aim of this study was to prove the function of mucus as defensive factor in rats. Method: The study was conducted in twenty white rats of the Sprague-Dawley strain at Department of Pathology and Clinical Reproduction, Bogor Agricultural University, between January and December 2008. The rat in the treatment group were given 400 mg aspirin diluted in aqua bidest through intra- gatric canules; while the control group received aqua bidest only once daily for 3 days. Necropsies, macroscopic and microscopic observation were performed by counting the number of Alcian blue- periodic acid Schiff-stained mucous cells at fundus/corpus and antrum/pylorus regions. Data analysis was performed using ANOVA and Duncan test. Results: The number of mucous cells with positive lesions in the treatment group was significantly different from the control group at both regions. There was no significant difference of negative lesions between treatment and control group at both regions. At antrum/pylorus region, there was no difference of negative lesions between treatment and control groups; however, both groups demonstrated significant difference of positive lesions in treatment group. Conclusion: In primary prevention for gastric mucosal lesions, there is no increasing number of mucous cells in normal mucosa. Increasing number of mucous cells is a secondary prevention against extended aspirin-induced gastric mucosal damage. Keywords: NSAIDs/ASA, mucus cells, gastric mucosal lesion, rat