S.E Estuningsih
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In vitro studies: The role of immunological cells in Indonesian thin tail sheep in the killing of the liver fluke, Fasciola Estuningsih, S.E; Wiidjajanti, S; Partoutomo, S; ., Spithill; Raadsma, H; Piedrafita, D
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.304 KB) | DOI: 10.14334/jitv.v7i2.284

Abstract

Previous studies have shown that Indonesian Thin Tail (ET) sheep exhibit high resistance to challenge with Fasciola gigantica when compared with Merino sheep, and this resistance is expressed in early infection. In order to study the role of the immune system in this resistance to ET sheep, in vitro studies were undertaken in the laboratory. In vitro study to confirm the ability of immune cells from ET sheep in the killing of F. gigantica larvae has been done by incubating immune cells and F. gigantica larvae together with immune sera or normal sera. The viability of the larvae was observed over a period 3 days incubation by observing their motility. The results showed that the cells isolated from F. gigantica- challenged ET sheep in the presence of immune sera from ET were able to kill 70% of the larvae. In contrast, cells from infected Merino were unable to kill a significant number of F. gigantica using the same sera source. It seems that the cytotoxicity was dependent on the presence of immune sera and ET peritoneal cells, suggesting the potential role of an antibody-dependent cell cytotoxic (ADCC) mechanism in the resistant ET sheep. Key words: In vitro, Fasciola gigantica, peritoneal cell, sheep gigantica.
Antibody fluctuations of infected cattle with Fasciola gigantica and the effect of triclabendazole treatment Widjajanti, S; Estuningsih, S.E; ., Suharyanta
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 4 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.383 KB) | DOI: 10.14334/jitv.v6i4.251

Abstract

Observation on the antibody fluctuations of infected cattle with metacercariae of Fasciola gigantica and the effect of triclabendazole treatment were made by means of ELISA technique. Seven cattle were infected with 700 metacercariae and one cattle remained uninfected, as negative control animal. Treatment with triclabendazole was given to 6 cattle, when the mean antibody levels of infected cattle reached the peak, and the other one remained untreated, as positive control animal. One week after treatment the mean antibody levels started to drop and then decreased gradually. After eight weeks of treatment, the mean antibody levels of the treated cattle reached the lowest level or the same value as before infection, thereafter, 6 cattle were reinfected with different dosages of metacercariae of F. gigantica. Two cattle were infected with 400 metacercariae, the other two were infected with 600 metacercariae and the rest of them were infected with 800 metacercariae. The results showed that the immunological responses of re-infected cattle are quicker (5 weeks after infection) and the peak of the antibody levels are higher (ELISA OD = 1.7) than after the first infection (11 weeks after infection and ELISA OD = 1.2). However, after re-infection, there were no significant different on the antibody fluctuations and antibody levels among the infected group, although those cattle received different dosages.   Key words: Antibody, cattle, Fasciola gigantica, ELI
The effect of the liver fluke Fasciola gigantica infestation on the leucocyte eosinophil cell profile on sheep Widjajanti, S; Estuningsih, S.E; ., Subandriyo; Piedrafita, D; Raadsma, H.W
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 3 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v9i3.408

Abstract

Eosinophil is one of the major leucocyte cell in the blood which specifically reacted on parasite infection, thus it is important to determine its profile against the F. gigantica infection. The aims of this study is to determine the differences of the eosinophil count profiles on the different breed of sheep infected with F. gigantica and its relation with the resistance of sheep bred against parasitic disease. Four groups of sheep consist of Indonesian Thin Tail (ITT) sheep, Merino sheep, backcross sheep (10 families) and F2 sheep were infected with 300 metacercariae of Fasciola gigantica. The total sheep used in this trial is 621. Those sheep were observed for 12 weeks and the blood samples were collected every 2 weeks after infection. The results showed that total eosinophil counts in all infected sheep increased after two weeks post infection and ITT sheep showed the highest counts. On the other hand, the mean fluke counts on ITT sheep is the lowest compared with the other groups of sheep. Merino and F2 sheep had the highest mean fluke counts. Three families of backcross sheep had the mean flukes count similar to ITT sheep and the other 7 families were similar to the Merino sheep. In conclusion, the highest total eosinophil count at the early stage of infection on ITT sheep might be related with the genetic resistance, which was showed by the lowest flukes count, and the resistance was inherited to some of the backcross sheep, which had similar flukes count with ITT sheep.   Key words: Fasciola gigantica, eosinophil, sheep
Comparative studies of resistance on Indonesian Thin Tail (ITT) sheep, St. Croix, merino and the crossbreed of ITT and St. Croix, against the infection of Fasciola gigantica Widjajanti, S; Estuningsih, S.E; Partoutomo, S; Roberts, J.A; Spithill, T.W
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 3 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.328 KB) | DOI: 10.14334/jitv.v4i3.158

Abstract

resistance is heritable. In order to re-evaluate this evidence, 20 ITT sheep were infected with 350 metacercariae of F. gigantica and for comparison, 10 St. Croix sheep, 10 Merino sheep and 20 crossbred of ITT x St. Croix sheep were also infected with the same dose of metacercariae. The results showed that ITT sheep was highly resistant than the other breed, whereas St. Croix and Merino sheep were susceptible. 60% of the crossbred were as resistant as ITT sheep and the other 40% were as susceptible as the St. Croix sheep. Thus, it is proposed that there might be a hereditary resistance factor such as a dominant gene which inducing the mechanism of resistance in ITT sheep, and there is some indication that IgG2 might act as a blocking antibody that interferes the mechanism of resistance.   Key words : ITT sheep, Fasciola gigantica, genetic resistance, dominant gene
In vitro studies: The role of immunological cells in Indonesian thin tail sheep in the killing of the liver fluke, Fasciola Estuningsih, S.E; Wiidjajanti, S; Partoutomo, S; ., Spithill; Raadsma, H; Piedrafita, D
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.304 KB) | DOI: 10.14334/jitv.v7i2.284

Abstract

Previous studies have shown that Indonesian Thin Tail (ET) sheep exhibit high resistance to challenge with Fasciola gigantica when compared with Merino sheep, and this resistance is expressed in early infection. In order to study the role of the immune system in this resistance to ET sheep, in vitro studies were undertaken in the laboratory. In vitro study to confirm the ability of immune cells from ET sheep in the killing of F. gigantica larvae has been done by incubating immune cells and F. gigantica larvae together with immune sera or normal sera. The viability of the larvae was observed over a period 3 days incubation by observing their motility. The results showed that the cells isolated from F. gigantica- challenged ET sheep in the presence of immune sera from ET were able to kill 70% of the larvae. In contrast, cells from infected Merino were unable to kill a significant number of F. gigantica using the same sera source. It seems that the cytotoxicity was dependent on the presence of immune sera and ET peritoneal cells, suggesting the potential role of an antibody-dependent cell cytotoxic (ADCC) mechanism in the resistant ET sheep. Key words: In vitro, Fasciola gigantica, peritoneal cell, sheep gigantica.
In vitro killing assays of antisera antibody sheep post-infected with Fasciola gigantica with the presence of macrophages cells against homologous and heterologous liver flukes Estuningsih, S.E; Widjajanti, S; Partoutomo, S; Spithill, T.W
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 3 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.594 KB) | DOI: 10.14334/jitv.v4i3.159

Abstract

The previous artificial infection known that the Indonesian Thin Tail (ITT) sheep was resistance against the liver fluke of Fasciola gigantica, the resistances occurred in the early infection. In order to observe the immune resistance, some in vitro studies were undertaken in the laboratory, to assay the ability of the antisera antibody of ITT sheep post-infected with F. gigantica, with the presence of macrophages cells in killing the homologous and heterologous liver flukes. The viability of liver flukes were observed within 24-72 hours of incubation period by observing their motility (motile flukes were designated live and non-motile once were death). The results showed that after 72 hours incubation, the motilities of the Newly Excysted Juvenile (NEJ) of F. gigantica incubated with the presence of post-infected sera and macrophages cells solution were significantly lower (P<0.05) than that of normal sheep sera. On the contrary, the post-infected sera and macrophages cells solution did not reduce the motilities of the NEJ of F. hepatica, and the death of these flukes were not significantly reduced (P >0.05). It seems that the occurrence of homologous antibody to the antigens is very important in the development of killing mechanism. The absence of homologous antibody did not reduce the number of flukes or the ability of macrophages cells in killing F. hepatica was not apparent.   Key words : In vitro studies, ITT sheep, macrophages cells, Fasciola gigantica, Fasciola hepatica
The immune responses on cattle and buffaloes infected with Fasciola gigantica before and after treatment Estuningsih, S.E; Widjajanti, S; ., Suhardono
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 4 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.779 KB) | DOI: 10.14334/jitv.v4i4.171

Abstract

The immune responses of cattle and buffaloes against the infection of liver flukes Fasciola gigantica were observed by ELISA. Six cattle and 6 buffaloes were infected orally with 700 metacercariae of F. gigantica, 2 cattle and 2 buffaloes were remained uninfected as control animals. Serum blood samples were collected weekly and the antibody levels were monitored before and after treatment. The results showed that the antibody level on cattle increased after 2 weeks post-infection, then gradually increased until reaching the peak at II weeks after infection. In the contrary, the antibody level on buffaloes appeared slower than on cattle, it increased after 13 weeks of infection and reach the peak at 25 weeks after infection. When the antibodylevels reach the peak, both groups of infected animals were treated with triclabendazole. After treatment, the antibody level oncattle was immediately decreased at one week of treatment, and gradually decreased up to the lowest antibody level at 7 weeks after treatment. The response of the treatment on buffaloes showed almost similar pattern, the antibody level decreased after one week of treatment, then remained constant for about 4 weeks, and reach the lowest antibody level at 7 weeks after treatment. It is concluded that the immune responses against the infection of F. gigantica in cattle and buffaloes are different, but the response of the treatment with triclabendazole are similar.   Key words: Fasciola gigantica, cattle, buffaloes, antibody levels, triclabendazole
The immune responses on cattle and buffaloes infected with Fasciola gigantica before and after treatment Estuningsih, S.E; Widjajanti, S; ., Suhardono
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 4 (1999): DECEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.779 KB) | DOI: 10.14334/jitv.v4i4.171

Abstract

The immune responses of cattle and buffaloes against the infection of liver flukes Fasciola gigantica were observed by ELISA. Six cattle and 6 buffaloes were infected orally with 700 metacercariae of F. gigantica, 2 cattle and 2 buffaloes were remained uninfected as control animals. Serum blood samples were collected weekly and the antibody levels were monitored before and after treatment. The results showed that the antibody level on cattle increased after 2 weeks post-infection, then gradually increased until reaching the peak at II weeks after infection. In the contrary, the antibody level on buffaloes appeared slower than on cattle, it increased after 13 weeks of infection and reach the peak at 25 weeks after infection. When the antibodylevels reach the peak, both groups of infected animals were treated with triclabendazole. After treatment, the antibody level oncattle was immediately decreased at one week of treatment, and gradually decreased up to the lowest antibody level at 7 weeks after treatment. The response of the treatment on buffaloes showed almost similar pattern, the antibody level decreased after one week of treatment, then remained constant for about 4 weeks, and reach the lowest antibody level at 7 weeks after treatment. It is concluded that the immune responses against the infection of F. gigantica in cattle and buffaloes are different, but the response of the treatment with triclabendazole are similar.   Key words: Fasciola gigantica, cattle, buffaloes, antibody levels, triclabendazole
Comparative studies of resistance on Indonesian Thin Tail (ITT) sheep, St. Croix, merino and the crossbreed of ITT and St. Croix, against the infection of Fasciola gigantica Widjajanti, S; Estuningsih, S.E; Partoutomo, S; Roberts, J.A; Spithill, T.W
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 3 (1999): SEPTEMBER 1999
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.328 KB) | DOI: 10.14334/jitv.v4i3.158

Abstract

resistance is heritable. In order to re-evaluate this evidence, 20 ITT sheep were infected with 350 metacercariae of F. gigantica and for comparison, 10 St. Croix sheep, 10 Merino sheep and 20 crossbred of ITT x St. Croix sheep were also infected with the same dose of metacercariae. The results showed that ITT sheep was highly resistant than the other breed, whereas St. Croix and Merino sheep were susceptible. 60% of the crossbred were as resistant as ITT sheep and the other 40% were as susceptible as the St. Croix sheep. Thus, it is proposed that there might be a hereditary resistance factor such as a dominant gene which inducing the mechanism of resistance in ITT sheep, and there is some indication that IgG2 might act as a blocking antibody that interferes the mechanism of resistance.   Key words : ITT sheep, Fasciola gigantica, genetic resistance, dominant gene
Antibody fluctuations of infected cattle with Fasciola gigantica and the effect of triclabendazole treatment Widjajanti, S; Estuningsih, S.E; ., Suharyanta
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.383 KB) | DOI: 10.14334/jitv.v6i4.251

Abstract

Observation on the antibody fluctuations of infected cattle with metacercariae of Fasciola gigantica and the effect of triclabendazole treatment were made by means of ELISA technique. Seven cattle were infected with 700 metacercariae and one cattle remained uninfected, as negative control animal. Treatment with triclabendazole was given to 6 cattle, when the mean antibody levels of infected cattle reached the peak, and the other one remained untreated, as positive control animal. One week after treatment the mean antibody levels started to drop and then decreased gradually. After eight weeks of treatment, the mean antibody levels of the treated cattle reached the lowest level or the same value as before infection, thereafter, 6 cattle were reinfected with different dosages of metacercariae of F. gigantica. Two cattle were infected with 400 metacercariae, the other two were infected with 600 metacercariae and the rest of them were infected with 800 metacercariae. The results showed that the immunological responses of re-infected cattle are quicker (5 weeks after infection) and the peak of the antibody levels are higher (ELISA OD = 1.7) than after the first infection (11 weeks after infection and ELISA OD = 1.2). However, after re-infection, there were no significant different on the antibody fluctuations and antibody levels among the infected group, although those cattle received different dosages.   Key words: Antibody, cattle, Fasciola gigantica, ELI