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Pengaruh Batang Bawah dan Jenis Tunas pada Mikrografting Manggis (Garcinia mangostana) secara In Vitro Handayani, Rd. Selvy; Poerwanto, Roedhy; Sobir, ,; Purwito, Agus; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 41, No 1 (2013): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The aim of this study was to investigate the effect of rootstock and shoot types on in vitro mangosteen micrografting.The experiment was arranged in completely randomized design (CRD) with two factors. The first factor was the rootstocktype, i.e. rooted planlet from the germination of quartered seed, and rooted planlet from the germination of undivided seeds.The second factor was the developmental phase of scion, i.e. dormant buds, and flush (had new leaf more than 2-4 mm). Theresults showed that rootstock derived from the germination of undivided seed had a higher success rate than other treatmentson all variables, except for number of new leaves. The use of flush as scion was better than dormant buds; flush resulted in ahigher percentage of successful micrograft and longer shoots. In vitro micrografting had a better growth rate than grafting at the same age. The results of anatomical observation conducted at four months after micrografting demonstrated that there was a good graft union, indicated by excellent fusion between rootstock and scion xylem tissues.Keywords: flush, in vitro, micrografting, rootstock, scion
Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 Lizawati, ,; Poerwanto, Roedhy; Sobir, ,; Rusmana, Iman; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Growth of mangosteen essentially depends on its root system.  Therefore, it needs technology to obtain stringer mangosteen root system.  The use of Agrobacterium rhizogenes bacterium is an alternative.  The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.  The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.  The experiment was arranged in completely randomized design with two factorial treatments.  The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).  The results showed that A. rhizogenes  of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.  Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method.   Key words :  Agrobacterium rhizogenes, Garcinia mangostana, inoculation
Karakter Anatomi Daun dari Kultur Tunas Artemisia annua L. Juliarni, ,; Dewanto, Hamami Alfasani; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 3 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Artemisia annua L. produce artemisinin, an endoperoxide sesquiterpene lactone, which is effective against resistant strains of Plasmodium falciparum, the malarial parasite. Artemisinin in foliar tissue are localized entirely in subcuticular space of capitate glandular trichomes. This research was performed to investigate the anatomical structures especially glandular trichomes which associated with artemisinin production in leaves of five different shoot culture clones (A, B, C, D, and E clones). Observation of anatomical characters of leaves was done by making cross-section, while observation of trichomes was performed using Scanning Electron Microscopy.  The leaves of five clones showed bifacial anatomical structure.  The leaf thickness of E clone was the highest (96.8 µm), while those of four other clones were relatively the same ranging from 62.8 µm to 66.6 µm. Glandular trichomes were distributed throughout the lamina of leaves with the highest distribution in adaxial parts of  the leaves. The size of uppermost secretory cells of glandular trichomes was relatively the same in five clones observed. There were variations in density of  glandular  trichomes in five clones observed. A and B clones had higher density of glandular trichomes i.e. 56.9 and 60.5/mm2, while three other clones had density which range from 43.0 to 49.7/mm2. It was suggested that A and B clones were the potential clones in producing artemisinin in vitro due to their larger leaf size and higher density of glandular trichomes.   Keywords :  Artemisia annua, shoot culture, anatomical structure of leaf
Potential of Sweet Potato Mutant Lines for Bioethanol Production Amsal, Aryanti; Yuniawati, Marina; Ermayanti, Tri Muji; Mulawati, Ika
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 7, No 2 (2011): Desember 2011
Publisher : BATAN

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Abstract

Shoots of sweet potato Sari variety were irradiated at the doses of 0, 10, 20, 30 and 40 Gy. Irradiated shoots were planted and selected to obtain better mutant lines than that of the parent plant. Ten mutant lines were from the fourth generation which better morphology and productivity than that of the parent plant. The bestproductivity was found at mutant line number 40-2 which was 717.50 g/plant compared to parent plant with 622.50 g/plant. The highest glucose and starch content obtained were at the dose of 20 Gy which were 8.85 and 28.56 %respectively. The mutant line of Sari sweet potato has a potential to produce bioethanol. The bio-ethanol production from those of mutant lines at a range of 15.02 to 19.46 % compared to 13.67 % in the parent plant. The mutant line number 20 was the best line to produce bio-ethanol. The aim of this experiment was to find mutant lines having potential to produce bio-ethanol
In vitro Seed Germination and Shoot Multiplication of Seven Endemic Subalpine and Alpine Plant Species Grown on Mount Jaya, Papua, Indonesia Ermayanti, Tri Muji; Hafiizh, Erwin Al; Mandessy, Ary; Setyadi, Gesang; Mukhsia, Andi
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

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Abstract

Exploitation on plant population may put the endemic plants into an endangered state, hence, these plants will need to be conserved. In order to pursue conservation on endemic plants, we conducted in vitro seed germination and shoot multiplication of seven alpine and sub-alpine species endemic to Mount (Mt.) Jaya, in Papua, Indonesia, i.e. Tetramolopium klossii, Deschampsia klossii, Papuacalia cartenszensis, Epilobium hooglandii, Gaultheria novoguinensis, Rhododendron correoides and Rhododendron culminicolum. These species are categorized as slow-growth plants found in higher altitude (over 3700 m above sea level) and low temperature of Mt. Jaya. Seeds were surface-sterilized using Na-hypochloride and germinated aseptically on Murashige and Skoog (MS) medium. Dytikinin benzyl adenine (BA) was used for shoot multiplication. Seedling cultures were maintained in a controlled environment with  continuous low light intensity (800 lux) and at temperature 26-27oC. Results showed that most species had more than 80% of germination rate on MS medium after a week in culture. BA was required to enhance shoots multiplication. Woody Plant (WP) (Lloyd & McCown, 1981) medium gave better shoot multiplication for R. culminicolum.
Induksi Akar Rambut Gandarusa (Justicia gendarussa Burm. f.) dengan Perlakuan Perbedaan Lama Waktu Infeksi Agrobacterium rhizogenes strain YM072001 dan A4T (The Induction of Gandarusa (Justicia gendarussa Burm. f.) Hairy Root under Different Infection Wahyuni, Dwi Kusuma; Nisa, Qonitatun; Purnobasuki, Hery; Ermayanti, Tri Muji; Prajoga, Bambang; Utami, Edy SW
JURNAL BIOS LOGOS Vol 5, No 2 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

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Abstract

Abstrak Penelitian ini bertujuan untuk menginduksi akar rambut eksplan daun Gandarusa (Justicia gendarussa Burm.f.) dengan perlakuan perbedaan lama waktu infeksi Agrobacterium rhizogenes strain YM072001 dan A4T dan mengevaluasi lama waktu infeksi terbaik untuk induksi akar rambut. Eksplan daun diinokulasi di media MS (Murashige dan Skoog) cair yang berisi bakteri Agrobacterium rhizogenes dengan OD600 = 0,1 selama 10, 20, 30, 40, 50, dan 60 menit. Kokultivasi di MS padat selama 2 hari, lalu disubkultur ke media MS padat. Data dianalisis secara deskriptif. Akar rambut mulai tumbuh pada minggu ketiga. Induksi akar rambut berhasil pada eksplan dengan perlakuan strain YMB072001 dengan lama waktu infeksi 20, 40, dan 50 menit. Strain A4T juga berhasil menginduksi eksplan membentuk akar rambut dengan perlakuan 10 menit. Lama waktu infeksi terbaik untuk strain YMB072001 adalah 20 menit dan untuk strain A4T adalah 10 menit. Kata kunci: Agrobacterium rhizogenes, akar rambut, Justicia gendarussa Burm. f., waktu infeksi   Abstract The objective of this study were to induce hairy root of Gandarusa leaf explants by differences of Agrobacterium rhizogenes infection time treatment and to evaluate the best infection time for induction. Leaf explants were inoculated on MS (Murashige dan Skoog) liquid medium with bacterial concentrations of OD600 = 0,1 for 10, 20, 30, 40, 50, and 60 minutes and 2 days co-cultivated on MS0 solid medium then sub cultured on MS0 solid medium. Data were analyzed descriptively. Hairy roots were growing on the third week. Explants were successfully induced by strain YMB 072001 at 20, 40, and 50 minutes treatment. A4T strain was successfully induced by 10 minute of treatment. The best infection time for hairy root induction in gandarusa leaf explants for YMB 072001 strains was 20 minutes and for A4T strain was 10 minutes. Keywords: Agrobacterium rhizogenes, hairy root, infection time, Justicia gendarussa Burm. f.
Isolation of anticancer compound of Artemisia cina hairy root and its inhibition activity on cervix cancer cells. ., Aryanti; Ermayanti, Tri Muji; Mariska, Ika; Bintang, Maria
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 4, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

The research of isolation of anticancer agent of A.cina hairy roots and its inhibition activity on cervix cancer cells have been conducted. Hairy roots transformed by Agrobacterium rhizogenes strains A4 and ATCC-15834 were then extracted by n-hexane and separated by column chromatography with variation of n-hexane/ethyl acetate as eluent. All samples include hexane extract and result of column chromatography tested to cervix HeLa Ohio cells with concentration of 50 μg/ml for hexane extract and 10 μg/ml for column chromatography respectively. The most active fraction was then tested by the concentration of 1 to 5 μg/ml. Confirmation of transformed root of A.cina was conducted by PCR analysis. The result of experiment shown that hexane extract of hairy root, normal root ( in vitro ), leaves of plant from green house as a control gave the inhibition value were about 84 %. The most active fraction from column chromatography was fraction E with IC50 at the concentration of 1 μg/ml and inhibition value was 95 %, the identification compound of this fraction was terpenoid group. The confirmation result showed that TL-DNA was transferred by 780 kb.Key words : anticancer, hairy root, Artemisia cina.
Antimalaria test of artemisia spp. on Plasmodium falciparum ., Aryanti; Ermayanti, Tri Muji; Priadi, Kartika Ika; Dewi, Rita Martaleta
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 2, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

The testing of inhibiton of Plasmodium falciparum by Artemisia annua, A.cina and A.vulgaris isolate and artemisinin content each of plants was conducted. Artemisia leaves extracted from n-hexane and then separated by column chromatography with n-hexane/ethylacetate as eluent. The column result tested to P.falciparum protozoa. The concentrations of agent were 100, 10, 1 and 0,1 μg/mL using sulphadoxin pyrimetamin as a control positive. Then 50 μl of cell suspension added to agent and incubated at 37oC for 30 hours and the number of live skizon calculated from 200 parasite asexual. The result showed that the increasing of agent concentration was increasing of parasite death. Percent of death parasite by agent of 100 μg/mL similar with positive control at the concentration of 300 μg/mL and the death by A.annua was 83.77%, A.cina 78.57 and A.vulgaris was 84.90% meanwhile positive control was 88.09%. The highest of artemisinin content found in A.annua was 4.99.Key words : anti-malaria, Plasmodium falciparum, Artemisia spp.
Variasi Jumlah Kromosom Planlet Taraxacum officinale Weber ex FH. Wigg Hasil Regenerasi in vitro dari Eksplan Akar, Helai Daun dan Tangkai Daun Ermayanti, Tri Muji; Lestari, Indah; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

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Abstract

Taraxacum officinale Weber ex F. H. Wigg. is a herbaceous medicinal plant species belonging family Asteraceasewhich has apomictic and poliploid characteristics. Multiplication of shoots using tissue culture was used to obtaindefine high quality seedlings, uniform, stable or free of diseases. However, changes in chromosome number canoccur in regenerated plants. The research aim was to determine the chromosome number of T. officinale plants regeneratedfrom culture in vitro using explants of roots, petioles and leaf blades. Therefore, selection of regenerantscan be done in order to find out transplants which have high yield of secondary metabolites. Analysis of chromosomenumber from root tips samples was conducted using 24 plantlets regenerated from root, 27 plantlets regeneratedfrom leaf blade, 21 plants regenerated from petiole and 102 roots of grown seeds using orcein squash method.The results showed that germinating seeds (control) and regenerated plants had variation in chromosome number.The range of chromosome numbers from regenerated plants were 2n=8-39, and cells with diploid number (2n = 2x= 16) was as most observed. The range numbers in germinated seeds were 2n=10-38, and cells with triploid number(2n = 3x = 24) was as most observed. This results proved that variation in numbers of chromosome was caused byapomixis and poliploid characteristics of the parent plant regenerated to their regenerants.Keywords : Taraxacum officinale. Weber ex F. H. Wigg, in vitro regeneration, variasi, chromosome
Peningkatan Pertumbuhan Kultur Tunas Stevia rebaudiana Bertoni pada Media dengan Peningkatan Kadar Vitamin dan Glisin serta Penggunaan Jenis Tutup Tabung Berbeda Ermayanti, Tri Muji; Rantau, Deritha Elly; Hafiizh, Erwin Al; Maulana, Evan
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

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Abstract

ABSTRACTStevia rebaudiana is plant species producing natural sweetener with has low calories. The species propagation could be done by tissue culture tecnique to obtain propagules with high quality and sustainability. Modification of media composition and in vitro environment will increase growth and vigority of explants so that they have high survival rate during acclimatization. The aim of this research was to increase growth of stevia shoot culture by increasing the level of vitamins in combination with different type of test tube covers. Stevia shoot tips were cultured on MS medium containing normal concentration of its vitamins (control treatment; Myo-inositol 100 mg/l; Nicotinic acid 0.5 mg/l; Pyridoxine-HCl 0.5 mg/l; Thiamine-HCl 0.5 mg/l and Glycine 2 mg/l), twice and 4 folds of vitamin levels, they were grown on culture tubes with Al-foil and ventilated-plastic with filter (2 cm diameter and pore size at 0,22 micron). Height of shoots, number of nodes, number of leaves, number of roots were observed every week till 8 weeks of culture. Biomass (fresh and dry weights) and chlorophyll level and acclimatization were done 8 weeks of culture. The results showed that type of culture tube covers affected significantly to all growth parameters, biomass as well as level of chlorophyll, meanwhile level of vitamins only affected number of nodes, shoots and roots. Interaction between vitamin level and covers types only occured for height of shoots and number of roots. Plantlets grown on medium containing 4 fold of vitamin level (Myo-inositol 400 mg/l; Nicotinic acid 2 mg/l; Pyridoxine-HCl 2 mg/l; Thiamine-HCl 2 mg/l and Glycine 8 mg/l) with ventilated-plastic cover had larger leaves compared to other treatments. All plantlets survived in a greenhouse.Keywords: Stevia rebaudiana, in vitro growth, increase in vitamin concentration.