KARTINI ERIANI
Department of Biology, Faculty of Mathematics and Natural Sciences of Syiah Kuala University

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Development of Domestic Cat Embryo Produced by Preserved Sperms ERIANI, KARTINI; BOEDIONO, ARIEF; DJUWITA, ITA; SUMARSONO, SONY HERU; AL-AZHAR, AL-AZHAR
HAYATI Journal of Biosciences Vol 15, No 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.643 KB) | DOI: 10.4308/hjb.15.4.155

Abstract

The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 oC, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 oC for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (P < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 oC in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos. Key words: gamet, preservation, in vitro fertilization
Potensi ekstrak daun flamboyan [Delonix regia (Boj. Ex Hook.) Raf.] terhadap peningkatan aktivitas dan kapasitas makrofag Rosnizar, Rosnizar; Maulida, Siti; Eriani, Kartini; Suwarno, Suwarno
Journal of BioLeuser Vol 1, No 3 (2017): Desember 2017
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

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Abstract

Aktivitas dan kapasitas makrofag merupakan faktor penting dalam menjaga sistem imun dalam tubuh. Beberapa senyawa berasal dari tumbuhan seperti daun flamboyan [Delonix regia (Boj. ex hook.) Raf.] dilaporkan banyak mengandung senyawa flavonoid yang berpotensi sebagai imunostimulan dan dilaporkan berpengaruh terhadap peningkatan aktivitas dan kapasitas fagositosis makrofag. Penelitian ini mengkaji tentang potensi  daun flamboyan sebagai agen imunostimulan pada peritonium mencit (Mus muculus) yang diinfeksi dengan bakteri staphylococcus aureus. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari lima perlakuan dan lima ulangan yaitu kontrol negatif (akuades) (P0), kontrol positif (Stimuno) (P1), ekstrak daun flamboyan pada dosis 250 mg/kg bb (P2), 500 mg/kg bb (P3) dan 750 mg/kg bb (P4). Mencit diberikan ekstrak selama 10 hari secara intubasi esofagus dan pada hari ke 11 diinfeksi dengan bakteri Staphylococcus aureus secara intraperitoneal. Jumlah makrofag yang aktif memfagositosis dan jumlah bakteri yang di fagosit menjadi parameter pengukuran. Hasil menunjukkan ekstrak daun flamboyan pada dosis 750 mg/kg bb (P4) memiliki nilai rata-rata aktivitas dan kapasitas fagositosis makrofag yang signifikan lebih tinggi berbanding P0, P1, P2, dan P3 (P < 0,05). Dari hasil ini disimpulkan bahwa ekstrak daun flamboyan pada dosis 750 mg/kg bb mampu mempengaruhi dan meningkatkan aktivitas dan kapasitas fagositosis makrofag.
Pengaruh Ekstrak Etanol Tumbuhan Anting-anting (Acalypha indica L.) Terhadap Kebuntingan dan Fetus Mencit (Mus musculus) Eriani, Kartini; Wiladatika, Wiladatika; Suangkupon, Razi
Journal of BioLeuser Vol 1, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

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Abstract

The whole plant Acalypha indica L. has been used as a traditional herbal medicine for generations by the society. However, the benefits of the whole plant Acalypha indica L. as a herbal medicine have not been known much, especially as an antifertility plant. It is assumed that the compunds contained within the ethanol extract of the whole plant Acalypha indica L. woulf affect upon one’s reproduction.  This research aimed to know the effect of the ethanol extract the whole plant Acalypha indica L. on pregnancy and fetal with obeserved on prenatal fetal (Mus musculus). It was carried out in july 2011 - february 2012 at Microtechnique Laboratory of  FMIPA Unsyiah. Completely randomized design was applied of the research, consisted of 4 treatments and 5 repetition of each treatment. The treatments were P0 (0 mg/ kg BW (control)), P1 (300 mg/ kg BW), P2 (600 mg/ kg BW), and P3 (900 mg/ kg BW) the ethanol extract of the whole plant  Acalypha indica L. that was given once a day for 7 days. The parameters were number of pregnancy, the average of fetal bodyweight, the number of fetal in uterine, and macroscopic defect. The data was analyzed by analysis of variance and chi-square, also continued by Tukey test. The result showed significant difference of number of pregnancy, the average of fetal bodyweight, and the number of fetal in uterine. The ethanol extract of the whole plant Acalypha indica L. extract could decrease the number of  pregnancy, the average of fetal bodyweight, the number of fetal in uterine on P1 (600 mg/ kg BW) and P2 (900 mg/ kg BW), also could not caused macroscopic defect on fetal.
IMMUNOSTIMULATORY EFFECT OF METHANOL EXTRACT OF FLAMBOYANT LEAF [Delonix regia (Boj. ex Hook.) Raf.] IN MICE Eriani, Kartini; Ainsyah, Ainsyah; Rosnizar, Rosnizar; Yunita, Yunita; Ichsan, Ichsan; Azhar, Al
Jurnal Natural Volume 18, Number 1, February 2018
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (513.493 KB) | DOI: 10.24815/jn.v18i1.9830

Abstract

Abstract. Flamboyant [Delonix regia (Boj. ex Hook) Raf.] leaf contains flavonoid compounds that are expected to have immunostimulatory effect. This research was done to determine the effect of flamboyant leaf extract on immune response by accessing the activity of immune cells and capability test the extract as immunostimulant in mice. Leaf extraction was done by maceration using methanol in the Laboratory of Biology of Chemistry Department, Faculty of Mathematics and Natural Sciences of Syiah Kuala University whereas animal treatment and testing were carried out Micro-technique Laboratory of Biology Department of the same faculty. This research used 20 male mice strain Swiss-Webster aged 7-8 weeks were randomly assigned to 4 treatment groups with five replications each. Group 1 (P0) was untreated control; group 1-3 were mice administration flamboyant leaf extract 250 mg/kg BW (P1), 500 mg/kg BW (P2), and 750 mg/kg BW (P3) per oral. The treatments were given for 14 days after one week of adaptation period. Blood samples were collected before and after extract treatment and used for leukocyte count analysis. Phagocytosis activity was accessed by carbon clearance assay on day 15. At the end of the study, all mice were sacrificed for spleen weight analysis. Data obtained was analyzed by Analysis of Variance followed by Tukey test (Leukocyte count and spleen weight) or regression analysis (carbon clearance). The results showed a flamboyant leaf extract administration resulted in increased leukocyte counts that were significantly different (p<0.05) between treatment groups.  Phagocytosis test indicated the extract had moderate to strong immunostimulatory effect whereas spleen weight analysis did not show any difference among treatment groups. In conclusion, flamboyant leaf methanol extract was able to increase immune cells and had potential immunostimulatory activity in mice.Keywords: Delonix regia, immunostimulant, leukocytes, lymphocyte proliferation.
PRODUKSI EMBRIO KUCING SECARA IN VITRO DARI SPERMATOZOA HASIL PRESERVASI MELALUI FERTILISASI MIKRO Eriani, Kartini; Sukra, Yuhara; Boediono, Arief; Djuwita, Ita; Sumarsono, Sony Heru
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.831 KB) | DOI: 10.21157/j.ked.hewan.v7i1.563

Abstract

Penelitian ini bertujuan mengetahui motilitas dan kemampuan memfertilisasi sperma kucing yang berasal dari epididimis yang disimpan pada suhu 4° C. Epididimis disimpan dalam media phosphate buffer saline (PBS) pada suhu 4 C selama 1, 3, dan 6 hari. Viabilitas spermatozoa diamati dengan pewarnaan hoechst-propidium iodine. Fungsi biologis spermatozoa dievaluasi melalui teknik kultur in vitro dengan fertilisasi mikro dan perkembangan embrio di dalam media kultur CR1aa. Hasil penelitian menunjukkan persentase spermatozoa hidup pada hari ke-1, 3, dan 6 penyimpanan masing-masing adalah 81,0; 71,7; dan 70,7% (duktus deferens), 84,0; 81,2; dan 63,2% (kauda epididimis), 84,0%; 75,0; dan 74,7% (korpus epididimis). Persentase pronukleus (PN) yang didapat dengan teknik intra cytoplasmic sperm injection (ICSI) menggunakan spermatozoa epididimis pada hari ke-1, 3, dan 6 hari penyimpanan masing-masing adalah 8,0; 10,0; dan 5,9%. Preservasi epididimis pada suhu 4 C dalam PBS dapat digunakan untuk fertilisasi dan menghasilkan embrio kucing secara in vitro.
MOLECULAR ANALYSIS OF PBMSP-1 GENE IN ERYTHROCYTE OF MICE INFECTED WITH Plasmodium berghei Rosnizar, Rosnizar; Eriani, Kartini
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.392 KB) | DOI: 10.21157/j.ked.hewan.v12i1.7452

Abstract

This study aimed to analyze MSP-1 gene in mice erythrocyte infected with Plasmodium berghei using polymerase chain reaction (PCR). The result showed the existence of 462 bp DNA band which was assumed to encode MSP-1 protein with molecular weight of 19 kDa that can only be found in erythrocyte infected with Plasmodium berghei. BLASTn analysis showed that PbMSP-1 obtained in this study have 100% similar identity with mRNA of PbMSP-1 partial sequence (462 bp), 93% similarity with PbMSP-1 complete sequence (5750 bp and 5376 bp), and 87% similarity with PbMSP-1 incomplete sequence (333 bp).
Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose Eriani, Kartini; Sari, Nisa; Rosnizar, Rosnizar; Dasrul, Dasrul; Suhartono, Suhartono; Rizal, Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.96 KB) | DOI: 10.15294/biosaintifika.v9i3.11426

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Aceh swamp buffaloes(Bubalus bubalis) are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloesafterthawing.This study used completelya factorial randomized design with nine treatments, and five replications.Fresh semen of the Aceh swamp buffalowere diluted by using a combination extender lactose cryoprotectants 0 mM (L0), 60 mM (L60), 120 mM(L120) and glycerol 3% (G3), 5% (G5), 7% (G7) with the equilibration of 4 hours.The results showed that the combination of cryoprotectants L120G7 influenced significantly (P < 0.05) on the quality of spermatozoa of the Aceh swamp buffalo(B. bubalis)after thawing.The percentage of sperm motility L120G7 (42.60 1.14);viability L120G7 (55.00 0.71);acrosome integrity L120G7 (52.00 0.71); and plasma membrane integrity L120G7(53.20 1.48). The combination of lactose cryoprotectants 120 mM (L120) and glycerol 7% (G7) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI).
Effect of Temperature Shock on the Triploidization Success of Seurukan Fish (Osteochilus vittatus) Eriani, Kartini; Syahrin, Alfis; Muchlisin, Zainal Abidin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8680

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Seurukan fish (Osteochilus vittatus) has many advantages, besides the fish also has disadvantages which are the slow growth, so the temperature shock of triploidization technique was expected to solve the problem. The objective of the present study was to obtain an effective temperature to increase of triploidization success of seurukan fish (Osteochilus vittatus). The experimental method and completely randomized design model were used in this study. Five levels of temperature shocks at three replicates were tasted: 4? (cold), 6? (cold), 28? (normal), 35? (heat) and 37? (heat). The sperms and eggs were fertilized in the plastic jar then a total 100 of fertilized eggs (zygotes) were taken randomly 3 minutes after fertilization and soaked in respective temperature for 90 seconds, and then incubated in incubation jars at the water temperature of 28-29?. The results showed that the temperature shock gave the significant effect on the hatching and the success of triploidization success (P?0.05), but did not give the significant effect the fertility and survival rates (P>0.05). The triploid fish can be achieved using cold and heat shock, but the higher triploid fish was recorded at 37? was the best temperature recommended for triploidization of Seurukan fish.
Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract Eriani, Kartini; Suryani, Irma; Azhar, Al; Nursanti, Risa; Ichsan, Ichsan; Boediono, Arief
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.15051

Abstract

Stem cell technology has great potential in the effort to cure degenerative diseases. This study was done to determine optimum dose of flamboyant (Delonix regia) flower extract to induce proliferation and differentiation of mice (Mus musculus) bone marrow mesenchymal-like stem cell. Bone marrow cells were collected from mice by aspiration. Cells suspension (1 x 106) were poured into petri dishes containing 2 ml of modified Dulbeccos Modified Eagles Media (mDMEM) and incubated overnight at 37 °C in a 5% CO2 incubator and microscopically observed. In quadriplicate, MSC were cultured in mDMEM containing D. regia flower extract of 0.0 (control), 0.4, 0.6, 0.8, and 1.0 mg/ml and incubated at 37 °C for 9 days. Population doubling time (PDT) and differentiated cell type were microscopically observed using HE staining on day 1 and 10. Data obtained were analyzed by ANOVA and Tukey test. The results showed that the addition of D regia flowers extracts 0.8 and 1.0 mg/ml significantly reduced PDT compared to that of 0.4, 0.6 and control. The extract, at 0.4 and 0.6 mg/ml, were able to induce MSC differentiation into fibroblast-like and nerve-like cells. In conclusion, D. regia flower extracts of 0.6, 0.8 and 1.0 mg/ml were able to stimulate MSC proliferation, but optimum dose for neurogenic differentiation was 0.6 mg/ml. This is the first to show potential of D. regia flower extract as neurogenic differentiatian inducer on mice MSC. These findings can be used as preliminary information for using the extract as cellular differentian inducer in basic and applicative reseach using stem cells.
Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation Dwitya, Yonadiah; Eriani, Kartini; Saputra, Hendra; Al-Azhar, Al-Azhar; Rizal, Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.954 KB) | DOI: 10.15294/biosaintifika.v11i1.18033

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Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender.