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Genetic Diversity and Phylogenetic Relationship of Kryptoterus limpok and Kryptopterus apogon from Kampar and Indragiri River Based on Cytochrome b Gene Elvyra, Roza; Duryadi Solihin, Dedy; Affandi, Ridwan; Junior, Zairin; Yus, Yusnarti
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol 16, No 1 (2009): Juni 2009
Publisher : Institut Pertanian Bogor

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Abstract

The utility of cytochrome b gene as a molecular marker to obtain genetic diversity and phylogenetic relationship among Kryptopterus spp. consist of Kryptopterus limpok and Kryptopterus apogon from Riau Province has been studied. The universal primers of (F) L14841 and (R) H15149 were used to amplify the cytochrome b gene. The results of multiple allignment were 159 nt (coding 53 amino acids). Kryptopterus limpok from Kampar and Indragiri River form a phylogeny cluster at 80% bootstrap value. Kryptopterus apogon from Kampar and Indragiri River form a phylogeny cluster at 99% bootstrap value.Keywords : cytochrome b, Kryptopterus limpok, Kryptoperus apogon.
ISOLASI DNA DAN AMPLIFIKASI FRAGMEN D-LOOP MITOKONDRIAL PADA IKAN Ompok hypophthalmus (Bleeker, 1846) DARI SUNGAI KAMPAR PROVINSI RIAU Rinarta, Della; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Total DNA molecule and fragment of the mitochondrial D-loop region is needed to determine the nucleotides sequence of mitochondrial D-loop region from lais danau fish (Ompok hypophthalmus). Therefore, this study aimed to isolate and amplify mitochondrial D-loop region that can be used as genetic information resources of O. hypophthalmus. The samples of O. hypophthalmus were taken from flood plain river in Riau, Kampar River. Tail muscle of O. hypophthalmus was used for DNA isolation. DNA molecules were amplified using PCR machine with L15926 and H00651primers. The total DNA of O. hypophthalmus was obtained in this study. The amplification process produced 153 bp fragment mitochondrial D-loop region of O. hypophthalmus.
ISOLASI DNA TOTAL IKAN SELAIS Ompok eugeneiatus (Vaillant 1893) DARI SUNGAI KAMPAR KIRI DAN INDRAGIRI HULU PROVINSI RIAU ', Tasiah; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

DNA isolation is a process to obtain pure DNA. The total DNA isolated includes nuclei and cytoplasm DNA (mitochondrial and chloroplast DNA). The total DNA can be used as stock for further genetic diversity analysis. Scientific information on genetic diversity of selais fish in Indonesia, particularly in Riau is still limited. One of the dominant species of selais fish in Riau is Ompok eugeneiatus. The research was conducted to obtain the total DNA of selais fish which can be used as a template DNA for genetic diversity analysis. The research had been conducted from December 2013 to May 2014 and the study site was located in Kampar Kiri and Indragiri Hulu rivers, Riau Province. Ten samples were isolated from each river. The DNA isolation process used isolation and purification kit (Qiagen DNeasy Blood & Tissue brand). The DNA isolation process was conducted based on the existing protocol. The total DNA were then migrated on 1% agarose gel and then visualized using UV transluminator. This research obtained the total DNA from each selais fish samples of Ompok eugeneiatus from the Kampar Kiri and Indragiri Hulu rivers with different quantity. The total DNA obtained were not fragmented.
ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Akmando, Rizka Suci; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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JOM FMIPA Volume 1 No. 2 Oktober 2014 224 ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Rizka Suci Akmando, Roza Elvyra, Dewi Indriyani RoslimMahasiswa Program S1 Biologi Dosen Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Kampus Bina Widya Pekanbaru, 28293, Indonesia rizkasuciakmando@yahoo.co.idABSTRACT Scientific information of mtDNA D-loop region in fish, Kryptopterus limpok, from Riau is unknown. Before having genetic information, total DNA isolation and amplification is necessary to be carried out. This study was aimed to obtain the total DNA molecules and DNA fragment of the mtDNA D-loop region in fish K. limpok from Sungai Kampar Kiri, Riau. Ten muscle samples were taken from fish collected from the river and used for total DNA isolation. Products of DNA isolation were amplified using the PCR technique with universal primers i.e. L15926 (F) 5’ TCA AAG CTT ACA CCA GTC TTG TAA ACC 3’ dan H00651 (R) 5’ TAA CTG CAG AAG GCT AGG ACC AAA CCT 3’. The PCR process consisted of pre-PCR at 940C for 5 minutes, 35 cycles of PCR consisted of three stages, i.e. as denaturation at 940C for 30 seconds, annealing at 590C for 1 minutes, elongation at 720C for 1 minutes, and post-PCR at 720C for 5 minutes. This study obtained the intact total DNA molecules, with the total DNA concentration of 100 μg/ μl. The length of mtDNA D-loop fragment was 153 bp.
ASPEK REPRODUKSI IKAN SELAIS KAPOREH (Ompok eugeneiatus) DI SUNGAI KAMPAR KABUPATEN KAMPAR PROVINSI RIAU Stephen H, Marhasak; Elvyra, Roza; ', Yusfiati
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Floodplain river ecosystem in Kampar River of Riau Province has a unique water characteristics. This area was the production center of freshwater fish in Riau. Selais fish was the maskot of Pekanbaru city and typical food from Riau. Selais Kaporeh(Ompok eugeneiatus) is one of fish in Buluh Cina and Mentulik and known to have high economic value in Kampar district. The objectives of this research were to determine the reproduction aspect of Selais Kaporeh including the level of gonads maturity(TKG), index of gonads maturity, fecundity and reproductive potential. This study had been conducted from February to April 2013 in Buluh Cina and Mentulik village. The samples were collected using survey method. The numbers of Selais Kaporeh collected were 155 fishes that consist of 137 females and 18 males, with sex ratio 7,61:1. The length of female fish ranged between 9,3-20,3 cm and weight ranged from 3,1-34,7 g.The length of males range were between 10-14,9 cm and weight range were 2.5-10.8 g. The gonads maturity level of female and male of Selais Kaporeh fish observed rangedfrom TKG I-IV. The index of gonads maturity (IKG) from female and male fish from TKG I-IV were between 0-10,82% and 0-4,4%. IKG increased following TKG and decreased after spawnings. Fecundity were 1825 eggs. Selais Kaporeh did not lay eggs simultaneously in one spawning season and they tend to spawn throughout the year.
TEKNIK ISOLASI DAN ELEKTROFORESIS DNA TOTAL PADA Kryptopterus apogon (Bleeker 1851) DARI SUNGAI KAMPAR KIRI DAN TAPUNG HILIR KABUPATEN KAMPAR PROVINSI RIAU Novitasari, Dede Aryani; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Kryptopterus apogon is one of freshwater fish species that was only found in floodplain river in Riau Province. Many species of lais fish are found in Riau Province rivers and morphologically has the same shape therefore it is necessary to find alternative markers to distinguish the species. The important step in genetic analysis is DNA isolation. This study aimed to isolate the total DNA from Kryptopterus apogon. The fish samples were taken from the Kampar Kiri and Tapung Hilir rivers. Isolation of total DNA was done by taking the muscle tissue from the fish tail (1 mm x 1 mm in size). Isolation of total DNA was done using DNA isolation kit (QIAGEN DNeasy Blood & Tissue). After that, the total DNA was checked on 1% agarose gel to estimate the quantity and quality of total DNA. Total isolated DNA of Kryptopterus apogon from two rivers had various quality and quantity. Tha total quantity of DNA molecules ranged from to 100-200 ng/μl, while the total quality was good enough or not fragmented. The DNA was clear, thick, and no smear.
JOM FMIPA Volume 1 No. 2 Oktober 2014 372 BIOLOGI REPRODUKSI IKAN LAIS PANJANG LAMPUNG (Kryptopterus apogon) DI SUNGAI KAMPAR KIRI DAN SUNGAI TAPUNG, PROVINSI RIAU Sari, Reni Mirsa; Elvyra, Roza; ', Yusfiati
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Fish of Lais Panjang Lampung is one of the unique fish from Pekanbaru and has high economic value but currently become endangered. This study was aimed to examine aspects of Lais panjang Lampung (Kryptopterus apogon) reproduction which include the development of gonads, gonad maturity index, sex ratio, spawning season, and spawning patterns. This study was conducted from October 2013 to March 2014 at Kampar Kiri River and Tapung. The number of fish obtained were 324 individuals with 124 males and 200 females. Total length and body weight of male and female fish ranged from 19 to 38.2 cm (31.13 to 209.06 g) and 20.5 to 33.4 cm (30.02 to 158.06 g), respectively. Sex ratios between male and female fish were 1:1,6. The males and females with TKG IV were commonly found in March (22.22%) and October (15.15%), respectively. Furthermore, the highest IKG of males and females fish were found in October, they were 0.066% and 0.5595% respectively. Fecundity of K. apogon ranged from 7294-35.742. Spawning patterns of Lais Panjang Lampung fish (Kryptopterus apogon) is a total spawner.
STRUKTUR USUS IKAN Ompok hypophthalmus (Bleeker 1846) DARI PERAIRAN SUNGAI SIAK KOTA PEKANBARU Firdasari, Venny; ', Yusfiati; Elvyra, Roza
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Ompok hypophthalmus is a freshwater fish which lives flood plain river. Siak river is one of flood plain river in Riau province. Siak river is very valuable for Pekanbaru people for household to industrial purposes. The household and industrial activity may cause the decrease of water quality. The contaminated water affects the living things in this area, such as O.hypophthalmus fish. Therefore, it is necesarry to detect the water quality decrease by observing the intestine structure of O.hypophthalmus. This study aimed to find out the histological structure of intestine tissue of Ompok hypophthalmus, especially which lives in the Siak I and Siak II bridge. This study was conducted from January to April 2014. Histological slide of O.hypophthalmus intestine were made using paraffin method and stained with Hematoxylin-Eosin (HE). The results showed that the intestine parasite infections was found in O.hypophthalmus, as well as lymphocyte stacking, villous unification, epithelium lysis, and lamina propria widening. The tissue damage in intestine might be due to the content of heavy metal above the normal standard.
MORFOMETRIK DAN MERISTIK IKAN BUNTAL MAS (Tetraodon fluviatilis H.B.) DI MUARA PERAIRAN BENGKALIS PROVINSI RIAU Ningsih, Tri Setia; Elvyra, Roza; ', Yusfiati
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Morphometric and meristic analysis of pufferfish (Tetraodon fluviatilis H.B.) in estuary of Bengkalisconducted in April-August 2014, which aims to determine the characteristics morphometric andmeristik pufferfish (T. fluviatilis H.B.) in three different habitats. This study used the method of direct data collection sampling from study sites. Sampling was carried out at three different stations are three i.e Pakning River, Bengkel River Station and the Jangkang River with 30 male and 30 female. The results showed morphometric characters pufferfish gold female and male on the Pakning River greater than the Bengkel River and Jangkang River with status allometric growth is positive, negative allometric and isometric. The results showed of morphometric analysis pufferfish (T. fluviatilis H.B.) using T-test showed a significant difference in the pufferfish gold male and female at each study site. Analysis of meristik pufferfish gold male and female at each study site showed a significant difference.
STRUKTUR INSANG IKAN Ompok hypophthalmus (Bleeker 1846) DARI PERAIRAN SUNGAI SIAK KOTA PEKANBARU Indrayani, Dwi; ', Yusfiati; Elvyra, Roza
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

This study aimed to observe the pathology of fish gill tissue Ompok hypophthalmus from Siak River around Siak I and Siak II bridge, Pekanbaru. The research was conducted from December 2013 to April 2014. The samples were prepared for histological observation using paraffin method and stained with Hematoxylin-Eosin (HE). Data were analyzed descriptively and quantitatively based on the histopathological evaluation and the value of the damage scoring. The results showed that the gill tissue structure of O.hypophthalmus fish changed. The damage in gill tissue found at Station I were 8,4% hyperplasia, 5,9% lamella fusion, 2,9% odema and 0,9% necrosis with total damage of gill tissue was 18,1%. Meanwhile, the damage in gill tissue found at Station II were 7,1% hyperplasia, 6,1% lamela fusion, 2,4% odema and 0,9% necrosis with total damage of gill tissue was 16,5%. The results of histopathological scoring below ≤25% indicated that the condition of the gill tissue was considered normal.