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Zingiber officinale, Piper retrofractum and Combination Induced Apoptosis and p53 Expression in Myeloma and WiDr Cell Lines EKOWATI, HENY; ACHMAD, ANISYAH; PRASASTI, EKA; WASITO, HENDRI; SRI, KADEK; HIDAYATI, ZULIA; EKASARI, TUTI
HAYATI Journal of Biosciences Vol 19, No 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.511 KB) | DOI: 10.4308/hjb.19.3.137

Abstract

In previous studies, Zingiber officinale, Piper retrofractum, and the combination showed cytotoxic activity, induced apoptosis, and p53 expression of HeLa, T47D, and MCF-7 cell lines. This study was conducted to investigate the cytotoxic and apoptotic activity of Zingiber officinale (ZO), Piper retrofractum (PR), and the combination as well as their effect to p53 expression on Myeloma and WiDr cells. The powder of ZO, PR, and ZO + PR combination (1:1) were macerated with 96% ethanol for 3 x 24 hours. MTT cytotoxic assay was performed on Myeloma and WiDr cell lines. Apoptotic cells were stained with ethidium bromide and acridine orange. Imunohistochemical expression of p53 was examined on Myeloma and WiDr cell lines. Doxorubicin was used as positive control in all assays. Results showed that ZO, PR, and ZO + PR combination had cytotoxic activity on Myeloma cells with IC50 of 28, 36, and 55 mg/ml respectively and WiDr cell lines with IC50 of 74, 158, and 64 mg/ml respectively, induced apoptotic activity, and increased p53 expression on Myeloma and WiDr cells. These results suggest that ZO, PR, and their combination induced Myeloma and WiDr cells in apoptosis through p53 expression.
An Extract of Zingiber officinale and Piper retrofractum Combination and Its Effect to Cancer Cell Line Ekowati, Heny; Septiyaningsih, .; Harwoko, .
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Chemotherapy may emerge sideeffect since it may treatinconveniently the synthesis of nucleic acids and proteins,both cancer cells or normal cells.Plants as a cancer therapy were expected to reduce this toxicityand side effects.Plants which used empirically for cancer therapy was Zingiber  officinale cv. Rubrum and Piper  retrofractum. This study was conducted to examine the cytotoxic activity of ethanolic extractc ombination of two plants in HeLa and T47D celllines.Zingiber  officinale cv.Rubrum,Piper  retro fractum and mixture (1:1)powdered then macerated with 96 % ethanol for 3x24 hours.Identification ofthe constituent that had potential anti cancer effect was usedTLC with silica GF254 as stationary phase,cytotoxic activity wasexamined by yellow MTT assay,the nanalyzed using probit.Apoptotic assay was performed by immunofluororescencemethod,using fluoro chromes ethidium bromide and acridineorange.The result showed that Zingiber officinalecv.Rubrumcontains terpenoids, while Piper  retrofractum contains alkaloids substance. The mixture showed cytotoxic activity against HeLa and T47D cell with IC50 33 and 53 6g/mL respectively.Theextract caused cytotoxic effect through apoptotic mechanism.
In Vitro Antioxidant Activity of Zingiber officinale, Piper retrofractum, and Their Combinations Wasito, Hendri; Ekowati, Heny; Hayati, Fitri Fauziah
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Many  plants  are  used  empirically  as  antioxidant.  Plants  that  are  frequently  used  in Indonesian  communities  are  Zingiber  officinale  and  Piper  retrofractum.  The  aim  of  this  research was  to  investigate  the  in  vitro  antioxidant  activity  of  single  ethanolic  extract  and  the combinations ofZ. officinale and P. retrofratum using free radical scavenging DPPH (1,1-diphenyl-2-picrylhydrazyl)  method.  Z.  officinale  and  P.  retrofratum  were  extracted  by  maceration  using 95%  ethanol  for  3  x  24  hours. Antioxidant  activity  was  evaluated  using  1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The concentration of the extract (μg/mL) that was required to scavenge 50% of free radicals (IC50)was calculatedusing the percent scavenging activities of six different  extract  concentrations.  The  results  showed  that  the  single  ethanolic  extract  of  Z. Officinalle produced the highest antioxidant activity with IC50of 56 μg/mL, while the antioxidant  activity of the single ethanolic extract of  P. retrofractum produced an IC50 of 3.445 μg/mL. The IC50 of combination of Z. officinale and P. retrofratum ethanolic extracts at concentration ratios of 1: 2, 1 : 1, and 2 : 1 were 148 μg/mL, 85 μg/mL, and 73 μg/mL.Key words: Zingiber  officinale, Piper retrfratum, Antioxidant, DPPH
Anti-Proliferative Activity of Nigella sativa Chloroform Extract on 7,12-Dimenthylbenz[a]anthracene Induced Female Rats Splenocyte Firdaus, Ahmad Fiki; Sobri, Iskandar; Ekowati, Heny
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Previous study reported that Nigella sativa has in vitro and in vivo cancer activity. This study was conducted to observe the effect of chloroform extract of Nigell sativa seed (NCE) on 7,12-Dimethylbenz[a]anthracene (DMBA)-induced female rats splenocyte. The experiment consisted of five groups, corn oil solvent control group, DMBA group, DMBA+250 mg/kgBW NCE, DMBA+500 mg/kgBW NCE and DMBA+750 mg/kgBW NCE. Extract was dissolved in corn oil and oral administered daily for 2 weeks before and during the DMBA induction. Observation of cell proliferation was performed using haematoxylin and eosin (H&E) and AgNOR stainings. H&E staining showed decreased necrocis activity extract groups compared to DMBA group. From AgNOR staining results, mean AgNOR (mAgNOR) of extract groups was less in number compared to DMBA group. The mAgNOR in corn oil solvent control group, DMBA group, DMBA+250 mg/kgBW NCE, DMBA+500 mg/kgBW NCE and DMBA+750 mg/kgBW NCE were 1.22, 1.91, 1.29, 1.36 and 1.33, respectively. Our current results showed that NCE reduces the proliferation of DMBA-induced rat spleenocytes. Thus, NCE has potency to be developed as a chemopreventive agent.Keywords : Nigella sativa, spleen, DMBA, anti-proliferative
Cost Analysis of Cancer Side Effects Therapy in Cancer Patients in Rumah Sakit Prof. Dr. Margono Soekarjo Purwokerto Pradana, Febrian; Purwonugroho, Tunggul Adi; Ekowati, Heny
Indonesian Journal of Cancer Chemoprevention Vol 3, No 3 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Cancer  is  the  leading  cause  of  death  in  Indonesia.  Generally,  cancer  therapy  use chemotherapy,  radiation,  surgery  or  combination  of  those.  Various  side  effects  caused  by cancer  therapy  make  the  use  of  additional  drugs.  This  leads  to  an  increase  in  total  cost incurred  to  cancer  patients.  The  purpose  of  this  study  was  to  describe  the  treatment  of cancer  therapy’s side effects  as  well  as  the  average  cost  of  side  effects  treatment,  and  its percentage  in  the  total  cost  of  cancer  patients  in  Rumah  Sakit  Margono  Soekarjo.  This research was a descriptive study with retrospective data  by total sampling method obtained from  patients’  medical  and  receipts  records  of  five  most  common  cancers  (breast, nasopharynx,  Non  Hodgkins  Lhympoma  (NHL),  ovarian,  and  cervix)  during  January-December 2010. The average cost of  patient’s side effects treatment was calculated by the average  cost  of  side  effects  compared  to  the  average  total  direct  costs  incurred  to  cancer patients.  The  samples  was  obtained  from  the  population  of  2261  patients  who  met  the inclusion criteria, that are 73 patients consist of 62 female patients and 11 male patients. The most  common  side  effects  of  cancer  therapy  were  nausea  and  vomiting  (59.80%),  nausea-vomiting,  anemia,  and  pain  (16.44%),  and  nausea-vomiting  and  anemia  (15.07%).  The  most used therapy was vitamin B complex followed by histamine-2 inhibitor. The average cost of side effects for breast cancer was Rp 1,715,373 (8.73%), nasopharynx was Rp 1,149,049 (6.98 %);  NHL  was  Rp  360,778  (1.97  %);  ovary  was  Rp  911,118  (3.61  %)  and  cervical  was  Rp 1,416,027 (8.87 %). The results indicated  that the hospital needs  to pay  more attention  on nausea-vomiting effect of chemotherapy, and also cervical cancer side effects therapy as the most costly.Keywords: Cost Analysis, Cancer, Side Effects, Prof. Dr. Margono Soekarjo Hospital
The effect of pharmacy visit to the potential drug interactions in the hospitalized geriatric patient at Dahlia ward Prof. Dr. Margono Soekarjo hospital Ekowati, Heny; P., Tunggul Adi; ., Trisnowati; Rahardjo, Budi
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 4, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp199-203

Abstract

Pharmaceutical care is the responsible provision of drug therapy for the purpose of achieving definite outcomes that improve a patient’s quality of life. Ward pharmacy is one way to realizing pharmaceutical care. This research’s aim was to study potential drug interactions in geriatric patient hospitalized at Dahlia ward Prof. Dr. Margono Soekarjo hospital, before and after ward pharmacy. The research used before and after study method, comparing potential drug interactions before and after ward pharmacy from March - June 2006. The different significance of drug interactions before and after ward pharmacy tested with Mann-Whitney test. Software which used in statistical analysis was SPSS (Statistical Product Social Science for) version 11.The study showed that total potential drug interaction before and after ward pharmacy were 3,69% and 5,12%. Drug interactions with significance rating 1, 2, and 3 before ward pharmacy were 0.08 ; 0.16 and 0.04 respectively ; while after ward pharmacy were 0.33 ; 0.14 and 0.09 respectively. The statistical analysis indicate that there was no a significance difference of drug interactions before and  after ward pharmacy (P> 0,05). The result was used to improve the system of pharmaceutical care in RSUD Prof. Dr Margono Soekarjo Purwokerto.Key words: ward pharmacy, geriatric patient, potential drug interaction
Potensi Antiproliferasi Jintan Hitam (Nigella sativa) pada Sel Lambung Tikus yang Diinduksi 7,12-Dimetil Benzena Anthrasena (ANTIPROLIFERATION EFFECTS OF NIGELLA SATIVA IN GASTRIC CELL RATS INDUCED 7,12-DIMETHYL BENZ (A) ANTHRACENE) Farihah, Rizky; Baroroh, Hanif Nasiatul; Ekowati, Heny
Jurnal Veteriner Vol 14, No 3 (2013)
Publisher : Jurnal Veteriner

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Abstract

Studies have been conducted to determine the potential antiproliferative effect of Nigella sativa seed(NSS) extract on 7,12- dimetilbenza[a]antrasena (DMBA)-induced female rats. The chloroform NSS extracteffect was observed on rat gatric cells. Sprague Dawley  female rats were divided into five groups, eachgroup consisted of 12 individuals. A control group was DMBA, Groups B, C and D were the groups treatedwith NSS extract with ranked dose 250 mg / kg, 500 mg / kg and 750 mg / kg and group E is the corn oilcontrol group. Histopathological observation of cells using H&E staining and cell proliferation activity wasobserved with the mean AgNOR (mAgNOR). mAgNOR values   were analyzed using Kolmogorov-Smirnovtest, than followed with ANOVA one way, and Tukey test HSD. The results showed H&E staining and  thechloroform exctract showed mAgNOR N.sativa semen were able to reduced gastric cell damage and lowergastric cell proliferation in DMBA- induced rat. The results showed that N.sativa potential to develop asa chemopreventive agent in gastric cancer.
Protective effects of Nigella sativa against 7,12-dimethylbenz [á] anthracene (DMBA) induced carcinogenesis in rats Alisah, Nur Fatmi; Baroroh, Hanif Nasiatul; Ekowati, Heny
Universa Medicina Vol 31, No 2 (2012)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2012.v31.88-95

Abstract

BackgroundLiver cancer is the third most common cause of death from cancer worldwide. Recently, natural products have been widely used as an alternative therapy for liver cancer. Previous studies have reported that Nigella sativa has chemopreventive activity in vitro and in vivo. The objective of this study was to evaluate the effect of a chloroform extract of Nigella sativa seeds (NSS) on female rat hepatocytes after administration of 7,12-dimethylbenz [á] anthracene (DMBA). MethodsThe experimental design comprised five groups of rats. Group I (DBMA control group) received oral DMBA at a dosage of 20 mg/kgBW twice weekly for five weeks, while group V (solvent control group) was given corn oil only. The other three groups received DMBA + NSS at dosages of 250 mg/kgBW, 500 mg/kgBW, and 750 mg/kgBW, respectively. Each group consisted 12 rats. The NSS extract dissolved in corn oil was administered daily by the oral route for 2 weeks before and subsequenyly during DMBA tumor induction. At the end of the study, rat livers were collected and stained with hematoxylin and eosin (H&E) and silver staining by the the AgNOR method. ResultsThere was a difference in liver tissue histopathological profile between the NSS, DMBA control, and the solvent control group. AgNOR counts in the DMBA control group, the DMBA+NSS 250 mg/kgBW group, DMBA+NSS 500 mg/kgBW group, and DMBA+NSS 750 mg/kgBW group were 1.79, 1.51, 1.41, and 1.35, respectively. ConclusionNigella sativa seed extract was able to reduce the liver damage and proliferation in rats induced by DMBA administration.
Combination of three species of Zingiberaceae prevents doxorubicin-induced hepatotoxicity Ekowati, Heny; Sarmoko, Sarmoko; Widiastuti, Retno
Universa Medicina Vol 32, No 1 (2013)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2013.v32.11 - 19

Abstract

BACKGROUND Doxorubicin as an anticancer drug has hepatotoxic side effects. Curcuma xanthorrhiza, Curcuma longa and Zingiber officinale are commonly used as herbals in Indonesia and around the world. Several compounds in these plants have antioxidant activities and are known to exhibit protection against doxorubicin-induced toxicities. This study aimed to observe the hepatoprotective effect of a combination of C. xanthorriza, C. longa, and Z. officinale extract on doxorubicin-induced hepatotoxicity in rats. METHODS A total of 28 Wistar male rats were divided into four groups: 1) control group (0.9% NaCl); 2) doxorubicin 5 mg/kg intraperitoneally (ip) four times in 14 days (days 1, 5, 9, 13); 3) doxorubicin + combination of C. xanthorriza, C. longa, and Z. officinale (temulawak, kunyit, and jahe merah, designated as Tekuja) 250 mg/kg/day orally for 14 days; and 4) doxorubicin + Tekuja extract 500 mg/kg/day orally for 14 days. Measurements of parameters based on liver histopathology and the parameters of serum alanine amino transferase (ALT) and aspartate amino tranferase (AST). RESULTS Doxorubicin caused significant elevation in serum ALT and AST enzymes after 14 days of treatment. Rats treated with doxorubicin + Tekuja extract 250 mg/kg/ day showed no histological changes, but had decreased levels of ALT and AST. CONCLUSION This study indicates that the combination of C. xanthorriza, C. longa, and Z. officinale has a protective effect in rats against liver damage induced by doxorubicin
Zingiber officinale, Piper retrofractum and Combination Induced Apoptosis and p53 Expression in Myeloma and WiDr Cell Lines EKOWATI, HENY; ACHMAD, ANISYAH; PRASASTI, EKA; WASITO, HENDRI; SRI, KADEK; HIDAYATI, ZULIA; EKASARI, TUTI
HAYATI Journal of Biosciences Vol 19, No 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.3.137

Abstract

In previous studies, Zingiber officinale, Piper retrofractum, and the combination showed cytotoxic activity, induced apoptosis, and p53 expression of HeLa, T47D, and MCF-7 cell lines. This study was conducted to investigate the cytotoxic and apoptotic activity of Zingiber officinale (ZO), Piper retrofractum (PR), and the combination as well as their effect to p53 expression on Myeloma and WiDr cells. The powder of ZO, PR, and ZO + PR combination (1:1) were macerated with 96% ethanol for 3 x 24 hours. MTT cytotoxic assay was performed on Myeloma and WiDr cell lines. Apoptotic cells were stained with ethidium bromide and acridine orange. Imunohistochemical expression of p53 was examined on Myeloma and WiDr cell lines. Doxorubicin was used as positive control in all assays. Results showed that ZO, PR, and ZO + PR combination had cytotoxic activity on Myeloma cells with IC50 of 28, 36, and 55 mg/ml respectively and WiDr cell lines with IC50 of 74, 158, and 64 mg/ml respectively, induced apoptotic activity, and increased p53 expression on Myeloma and WiDr cells. These results suggest that ZO, PR, and their combination induced Myeloma and WiDr cells in apoptosis through p53 expression.