Jusuf S. Effendi
Department of Obstetrics and Gynecology, Padjadjaran University, Bandung

Published : 6 Documents
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Comparison of Fibrinogen Level Changes between Pregnancy with History of Abortion and Normal Pregnancy

Indonesian Journal of Obstetrics and Gynecology Vol. 35. No 2. April 2011
Publisher : Indonesian Journal of Obstetrics and Gynecology

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AbstractObjective: To know the change of fibrinogen level in pregnancy with history of abortion and normal pregnancy on 6 - 8 weeks pregnancy and 10 - 12 weeks pregnancy.Method: This is a comparative study analytic comparative with cross sectional method on both groups, pregnancy with history of abortion and normal pregnancy. Observe the changes of fibrinogen level on 6 - 8 weeks pregnancy and then on 10 - 12 weeks pregnancy in pregnancy with history of abortion and normal pregnancy.Result: Comparison of mean fibrinogen level between 6 - 8weeks pregnancy and 10 - 12 weeks pregnancy, on both group showed that the fibrinogen level in the group with history of abortion increased 9.6% and in the group of normal pregnancy increased 11.4%. The raise on both group was not significant stastistically (p= 0.810). The raise fibrinogen level on normal pregnancy was significant (p < 0.001), while on pregnancy with history of abortion is not significant (p = 0.255). All patterns of raise and fall on fibrinogen level on both groups were not stastistically significant (p > 0.005).Conclusion: Fibrinogen level on 10 - 12 weeks pregnancy was not lower than on 6 - 8 weeks pregnancy in pregnancy with history of abortion. There was no significant raise changes in fibrinogen level on both groups.[Indones J Obstet Gynecol 2011; 35-2: 53-6]Keywords: abortion, fibrinogen, haemostasis, history of abortion, normal pregnancy

Effects of Peritoneal Fluid on Sperm Motility and Viability in Endometriosis

Indonesian Journal of Obstetrics and Gynecology Vol. 35. No 1. January 2011
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Objective: To know the effects of peritoneal fluid on sperm motility and viability in patients with endometriosis. Design/data identification: This was a laboratory experimental study to peritoneal fluid from endometriosis and non-endometriosis patients who underwent surgery in Dr. Hasan Sadikin Hospital and around with endoscopy facility which fulfill inclusion and exclusion criterias. Experiments was performed in ASTER Fertility Clinic, Dr. Hasan Sadikin Hospital.Method: Semen samples were normozoospermic of which has been prepared using swim up method with sperm count 3 x 106/ml. The sperm were exposed to peritoneal fluid from endometriosis and non-endometriosis patients and analyzed at h 0, 1, 3, 6, and 24 to see the difference of sperm motility and viability postincubation with endometriosis peritoneal fluid. The sperm viability was detected using trypan blue 0.4%.Result: Exposure of sperm to peritoneal fluid reduced sperm motility significantly from the h 6 observation (Zw = 2.17; p = 0.03) and the h 24 (Zw = 2.35; p = 0.01). The sperm viability which incubated with endometriosis peritoneal fluid reduced significantly from h 6 observation (Zw = 1.99; p = 0.04) and the h 24 (Zw = 2.55; p = 0.01).Conclusion: The endometriosis peritoneal fluid reduced the motility and viability of the sperm began from the h 6 postincubation. This indicate the possibility of involvement of endometriosis peritoneal fluid to infertility.Keywords: sperm motility, sperm viability, endometriosis

The Level of TNF-α Serum is Higher in Incomplete Abortion Compared to Threatened Abortion and Normal Pregnancy

Indonesian Journal of Obstetrics and Gynecology Vol. 35. No 3. July 2011
Publisher : Indonesian Journal of Obstetrics and Gynecology

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AbstractObjective: To analyse the comparison of TNF-α serum level between threatened abortion, incomplete abortion, and normal pregnancy.Method: Analitic observational study with cross-sectional design. It was involving 48 samples who met inclusion criteria by consecutive sampling during April 11- July 12, 2011 in Dr. Hasan Sadikin Hospital and networking hospital. Threatened abortion group was followed up to 20 weeks of pregnancy. Statistical analysis used are t test, Kruskal Wallis, Mann Whitney, and Wilcoxon.Result: Mean of TNF-α serum level is highest in incomplete abortion 6.10 ± 1.66 pg/ml compared to threatened abortion 3.83 ± 1.00 pg/ml and normal pregnancy 2.72 ± 0.4 pg/ml. Mean serum level of TNF-α in poor outcome (7.16 ± 1.61 pg/ml) threatened abortion is higher compare than good outcome threatened abortion (3.15 ± 0.24 pg/ml) (p < 0.05).Conclusion: Mean TNF-α serum level of threatened abortion is lower compared to incomplete abortion but higher than normal pregnancy. Mean TNF-α serum level in poor outcome threatened abortion is higher compared to good outcome threatened abortion.[Indones J Obstet Gynecol 2011; 35-3: 119-21]Keywords: threatened abortion, incomplete abortion, TNF-α

Perbedaan Indeks Apoptosis Plasenta Antara Preeklamsi dan Kehamilan Normal serta Hubungannya dengan Berat Badan Lahir dan Tekanan Darah Ibu

Majalah Kedokteran Bandung Vol 1, No 42 (2010)
Publisher : Majalah Kedokteran Bandung

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AbstrakAngka kejadian preeklamsi di Indonesia pada tahun 1980-2001 adalah 6-8% dari seluruh kehamilan, dengan angka kematian maternal 9,8-25%. Terdapat dugaan bahwa pada preeklamsi terjadi peningkatan apoptosis akibat turunnya ekspresi Bcl-2 sebelum usia kehamilan aterm. Tujuan penelitian ini untuk menentukan perbedaan antara indeks apoptosis (IA) plasenta pada preeklamsi dan kehamilan normal serta hubungannya dengan berat badan lahir bayi dantekanan darah ibu. Desain penelitian adalah observasional analitik secara potong silang, terhadap 63 subjek yang terdiri dari 32 ibu preeklamsi dan 31 ibu hamil normal. Penelitian dilakukan di Rumah Sakit Dr. Hasan Sadikin. Selama periode bulan April-Juni 2008, dilakukan pemeriksaan IA (caspase 3) secara imunohistokimia di laboratorium Patologi Anatomi. Analisis data ditentukan dengan menggunakan tes Chi kuadrat dengan p<0,05 sebagai batas kemaknaan. Pada kelompok preeklamsi terdapat peningkatan IA (21,9%), sebaliknya tidak terdapat peningkatan IA pada kehamilan normal (0%) (p=0,006). Terdapat perbedaan IA menurut berat badan lahir bayi yang bermakna pada preeklamsi (p=0,046), sedangkan pada kehamilan normal tidak bermakna (p>0,05). Tidak terdapat perbedaan IA menurut tekanan darah ibu pada kedua kelompok (p>0,05). Disimpulkan bahwa terdapat perbedaan indeks apoptosis antara preeklamsi dan kehamilan normal, sedangkan hubungannya dengan berat badan lahir hanya berbeda pada preeklamsi. [MKB. 2010;42(1):1-5].Difference of Placental Apoptosis Index in Preeclampsia and NormalPregnancy and Its Correlation with Birth Weight andMother´s Blood PressureThe incidence of preeclampsia in Indonesia (1980–2001) was around 6-8% of the total pregnancies with maternal mortality rate 9.8-25%. In preeclampsia, an increase of apoptosis occurs as a result of a decrease of Bcl-2 expression before a pregnancy reached full term. The objectives of this study were to determine placental apoptosis index (AI) inpreeclampsia and normal pregnancy, and its correlation with birth weight and mother´s blood pressure. This study was an observational analytical study using cross sectional design among 63 patients consisted of 32 preeclampsia and 31 normal pregnancies. This study was performed at Dr. Hasan Sadikin Hospital, during April-June 2008.Immunohistochemical analysis was used to examine the placental AI (caspase 3). Chi square test was used to analysed the result using p<0.05 for the significant difference. AI was significantly higher in preeclampsia group (21.9%) than in the normal pregnancies (0%) (p=0.006). There was significantly difference of AI with birth weight in preeclampsia with p=0.046. On the otherhand, there was no significantly difference of AI with birth weight in normal pregnancies. There was no significantly difference of AI with mother´s blood pressure in the two

Role of nuclear factor kappa beta, tumor necrosis factor α, and cyclooxygenase-2 in preterm labor

Medical Journal of Indonesia Vol 23, No 4 (2014): November
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Background: The pathway of tumor necrosis factor alpha (TNFα), nuclear factor kappa beta (NF-κB), and cyclooxygenase-2 (COX-2) activation in releasing prostaglandins is suggested to be crucial for initiating labor in the pathogenesis of preterm labor. The aim of the study was to know whether there were mean differences of NF-κB, TNFα, and COX-2 expressions between preterm and term labor and also to know the correlation among them in preterm labor.Methods: A case-control study was performed from May 2013 to February 2014 in Arifin Achmad Hospital, Pekanbaru. There were 30 subjects with preterm labor as cases and 30 with normal labor as controls. All subjects had singleton gestation with maximum parity was three, age limit of 35 year-old, and spontaneous labor in both groups. Placental tissue was collected from all subjects and evaluated with hematoxylin eosin staining. The expressions of TNFα, NF-κB, and COX-2 in the tissue were assessed with immunohistochemical staining by counting the percentage of smeared cells by two experts. The expressions of TNFα, NF-κB, and COX-2 between case and control were compared using t-test and the correlation was analyzed with Pearson correlation coefficient.Results: Mean (SD) of expressions of TNFα (93.05% [12.68] vs 49.11% [27.33]), NF-κB (42.46% [27.29] vs 13.66% [17.77]), and COX-2 (88.75% [10.86] vs 46% [30.36]) were significantly higher in the preterm labor compared to term labor (p = 0.001). There was significant correlation between TNFα and NF-κB expression (r = 0.385; p = 0.036) and no correlation was found between NF-κB and COX-2 (p = 0.982) in preterm labor.Conclusion: High expressions of TNFα, NF-κB, and COX-2 in preterm labor showed to contribute in the onset of preterm labor. High TNFα may suggest that infection was a leading cause of preterm labor. This is supported with an increase in NF-κB activation will increase COX-2 and subsequently prostaglandins that result in premature labor.

Hubungan Umur Ibu, Paritas dan Penolong Persalinan dengan Kematian Neonatal di Wilayah Kerja Puskesmas Kabupaten Banjarnegara tahun 2013

JURNAL KESEHATAN REPRODUKSI Vol 3, No 1 (2016)
Publisher : IPAKESPRO

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Background: The neonatal mortality rate is Banjarnegara district is high compared with a target of IMR in Central Java province. In 2012 the neonatal mortality rate in Banjarnegara reached 316 cases, whereas in 2013 there were 115 cases in all health centers working area. The high neonatal mortality rate in Banjarnegara influenced by the persistence of early marriage in woman, birth attendant by non skilled health worker (dukun bayi) and women’s parity more than 4.Objective: The purpose of this study is to analyze the correlation between maternal age, parity and birth attendants with the incidence of neonatal mortality and examine the most dominant factors influencing the incidence neonatal mortality in the Health Center working area in Banjarnegara.Method: This study is a case control study design with retrospective approach. The data consists of perinatal verbal autopsy and medical records as many as 136 respondents consisting of 68 mothers who gave birth to her child and neonatal death and 68 maternal and child alive. Subjects were mothers who gave birth a baby who died aged 0-28 days.Result and Discussion: Statistical analysis using Chi Square, Mann Whitney, and multiple logistic regression. The results showed maternal age and parity variables were not significantly corerelated with neonatal mortality (p> 0.05), the variables significantly corerelated with neonatal mortality was birth attendants with p = 0.001. The results of logistic regression showed the strength of the corerelation from the most dominant variable to the smallest variable were birth attendants with OR: 5.64 (95% CI: 1.81 to 17.4), maternal age OR 3.97 (CI 95%, from 1.54 to 10.22), and parity OR: 0.32 (CI 95% 0.12 to 0.87).Conclusion: In conclusion, there was no relationship between maternal age and parity with neonatal mortality but there was a relationship between birth attendants with neonatal mortality. Based on the results of the multivariable analysis, birth attendants’ effect on the incidence of neonatal deaths was 5 times greater than mother’s age and parity.Keywords: Neonatal mortality, maternal age, parity, birth attendants