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ABILITY OF ISOLATE PROBIOTIC BACTERIA CANDIDATE FROM KAKAP PUTIH (Lates calcarifer) IN PRESSING GROWING OF BACTERI Vibrio alginolyticus IN CO-CULTURE Situmeang, Christine M; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Three candidates of probiotic bacteria isolated from kakap putih (Lates calcarifer) had been examined for their antagonism against Vibrio alginolyticus. However, the ability to suppress the pathogen in co-culture system has not been tested. This research aimed to test the ability of probiotic candidates to suppress the growth of V. alginolyticus in co-culture trial. Three probiotics candidates (KP1, KP2, and KP3) were used in this experiment method. Four treatments consisted of P1 (broth culture of KP1 + V. alginolyticus), P2 (KP2 + V. alginolyticus ), P3 (KP3 + V. alginolyticus), P4 (V. alginolyticus) as control were prepared in triplicate. Total counts of each probiotic candidate and V. alginolyticus was calculated after 48 hours culture incubation. KP1 isolate showed highest ability suppress the growth of V. alginolyticus, followed by KP3 and KP2 isolates.Keywords: Probiotics, Kakap Putih, Growth, V. alginolyticus, Co-culture
Effect of Addition “Attack” Detergent at Different Concentration and Exposure Time on Heterotrophic Bacterial Growth in Sea Water Pakpahan, Roiman; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research aims to determine the effect of “Attack” detergent addition at different concentration and exposure time on heterotrophic bacterial growth in sea water. The research was conducted from February to April 2017 used experimental method. The results showed that the highest number of heterotrophic bacteria was observed on the A5 day 1st exposure treatment of 6,69 x 107 (CFU/ml) and the lowest was in the 16th day of exposure treatment of 0,098 x 107 (CFU/ml). At day 1st, the value of the correlation coefficient (r) obtained by 0.277 showed the relationship between two variables with positive linearity, while day 6th to day 21th, the correlation coefficient (r) are 0,982, 0,9426, 0,9778 and 0,986, showed the relationship between moderate two was very strong variables with negative linearity. The regression relationship between exposure time and bacterial growth showed a strong correlation with negative linearity, where the coefficient value (r) was 0,87. Significant treatment of detergent concentration that was equal to 0,00 and exposure time that was equal to 0,00 < α (0,05). The bacterial growth on treatment A1 (control) was significantly different with treatment A2, A3, A4 and A5. Bacterial growth at day 1st was significantly different from 6th, 11th, 16th and day 21th. The result of this research showed that there were a effect of “Attack” detergent addition with different concentration and exposure time to the growth of heterotrophic bacteria in seawater.Keyword: Detergent, Concentration, Exposure, Growth, Heterotrophic Bacteria
Isolation And Heterotrophic Bacteria Antagonism Test From Estuary Of The Siak River with Different Salinity Yanti, Syaputry Lita; Feliatra, Feliatra; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5, No 1 (2018): Wisuda April Tahun 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT The research was conducted from July to September 2017 in Estuary of Siak River. The purpose of this research is to examine thepopulation of heterotrophic bacteria in Estuary of Siak River, and heterotrophic bacteria antagonism against pathogenic bacteria (Vibrio sp, Aeromonas sp and Pseudomonas sp). And than identified by phenotype and genotype. This research use survey method. The samples analisis were conducted at the Marine Microbiology Laboratory of Marine Science Department of Fisheries and Marine Faculty and DNA analisis conducted at the Genetic Laboratory of Biology Department Mathematic and Natural Sciences. The result of antagonism test from 25 isolate heterotrophic bacterial was found 10 isolates which have ability to inhibit the growth of pathogenic bacteria (Vibrio sp, Aeromonas sp and Pseudomonas sp). From Vibrio sp bacteria the mean of clear zone is 1P11 isolate of 20,3 mm and the smallest is 1P19 isolate of 5 mm. Aeromonas sp bacteria the largest clear zone is 2P20 of 9,3 mm and the smallest is 1P12 isolate of 3,3 mm. Pseudomonas sp bacteria the largest clear zone is 1P12 isolate of 17 mm and the smallest is 1P11 and 2P22 isolate of 8,3 mm. the result of molecular identification using PCR 16S rRNA method and BLAST analisis showed 10 isolates of heterotrophic bacteria having kinship with bacteria of the genus Bacillus that is Bacillus cereus (1P11, 1P12, 1P14, 1P15, 1P19, 2P20, 2P21, 2P23), Bacillus toyonensis (2P22) and Virgibacillus salarius (1P18). Keyword : Heterotrophic Bacteria, Isolation, Antagonism test, Identification
Relationship between Surfactant and Population of Heterotrophic Bacteria in Estuary of Dumai River Dumai City Riau Province Rivai, Aqil Fikar; Nedi, Syahril; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted on April 2017. Sampling was conducted in estuary of Dumai River, Dumai City, Riau Province. A study aims to determine the relationship between concentration of surfactant and population of heterotrophic bacteria in the estuary waters of Dumai River Dumai City Riau Province. Sampling was done at 6 sampling points (stations). The analysis of surfactant concentration was conducted at the Chemical Laboratory of the Sea and the total analysis of heterotrophic bacteria at the Marine Microbiology Laboratory of Department of Marine Science Faculty of Fisheries and Marine University of Riau, Pekanbaru. The results showed that the highest concentration of surfactant was found at Station 5 (0.8538 mg / l) and the lowest at Station 1 (0.2025 mg / l). Then, the logarithm of the highest average total colony was found at Station 1 (6.18 cells / ml) while the lowest at Station 5 (5.66 cells / ml). The correlation concentration models of surfactant with the total colony using regression was negative correlation Y = 6.3745 - 0.7882 (X), R2 = 0.8622, r = 0.9285 showed that the value of surfactant concentration (X) increased by 1 unit will decrease the total of colony (Y) was 0,7882 cells/ml, with the effect of surfactant to bacteria equal 85% and the level of closeness of the relationship was 93%.Kata Kunci : Surfactant, Heterotrophic bacteria, Estuary of Dumai River
Experiment of Rinso Detergent’s Effect On the Growth of Heterotrophic Bacteria in Sea Water Tanjung, Cahyani Fitrah; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted in March-April 2017 at Marine Microbiology Laboratory, Faculty of Fisheries and Marine University of Riau. The purpose of this study is to determine the different concentration of detergent (0 ml/L, 1.5 ml/L, 3 ml/L, 4.5 ml/L, 6 ml/L) in different observation time (0, 5, 10, 15, 20) on the growth of heterotrophic bacteria in sea water. Completely randomized design was used in this experimental method. The results showed that bacterial growth of all treated samples decreased on the 5th day of incubation. However, the population began to increase on the 10th day of incubation. The count of maximum bacterial growth was 1.46 x 109 found in the 4.5 ml/L treated detergent, and the lowest growth was 3.73 x 107 in the 1.5 ml/L treated detergent. Statistical analysis (ANOVA) showed that the concentrations in different observation times on the growth of heterotrophic bacteria showed significant effect and the value was (P <0.005).Keywords: heterotrophic bacteria, population growth, rinso, sea water.
Growth Of Heterotrophic Bacteria At Sea Water Polluted By ‘Surf’ Detergent in The Water of Kayu Ara Village Siak Regency Riau Province Novian, Dewan Pratama; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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The research was conducted from February 2016 to April 2017 and was aimed to determine the effect of adding different concentrations of detergent on the growth of bacterial populations in the ocean water column and to see the ratio of bacterial persisted capability in different lengths of dissection. This research used experimental method with Completely Randomized Design. Seawater samples were analyzed at Marine Microbiology Laboratory, Fisheries and Marine Faculty of Riau University. Based on the results of the study, from day 0 untill day 10 after given detergent with different concentrations, bacteria experienced a decreasing trend of growth, in day 15 untill day 20, the trend of bacterial growth contaminated by detergent with different concentration relatively increased to stagnant. Based on the result of this study the addition of different detergent concentration in each treatment affect the growth of heterotrophic bacteria population in the sea water, where the higher concentration of detergent added the lower the ability of bacteria to grow and the ability of heterotrophic bacteria to survive at different concentrations of detergent, it can be concluded that the ability of heterotrophic bacteria to grow from days 0 to 20 has decreased in each of its concentrations.   Keywords: heterotrophic bacteria, Surf detergent.
Gastroesophageal reflux disease with Thal fundoplication Effendi, Irwan; Jurnalis, Yusri Dianne; Sayoeti, Yorva; Yusuf, Yusirwan
Paediatrica Indonesiana Vol 50 No 6 (2010): November 2010
Publisher : Indonesian Pediatric Society

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Gastroesophageal reflux (GER) is a common phenomenon among heathy infants, with approximately 50% of infants aged 0 to 3 months and 67% of infants aged 4 months experiencing at least one episode of vomiting per day. GER defined as regurgitation of gastric contents into the esophagus or mouth. GER typically improves through the first postnatal year, with only 5% of healthy 12 month old infants experiencing vomiting.1,2 Complicated GER or gastroesophageal reflux disease (GERD) has been reported to affect up to 8% of infants and children with GER.l,3 Antireflux procedures (ARPs) are increasingly offered to control GERD symptoms. We report a case of a baby with GERD and treated with ThaI fundoplication procedures. Fundoplication procedure is rarely performed in management of GERD, and this is the first fundoplication procedure in children with GERD at M. Djamil Hospital.
The Growth of Biomass Spirulina platensis with Different Nutrition Giving with Indoor and Semi Outdoor Scale Systems Nainggolan, Judita Grace Merry; Tanjung, Afrizal; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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 ABSTRACT                Microalgae are very potential to be developed as biofuel feedstocks when compared to food crops because they have several advantages such as rapid growth, high productivity, enabling the use of freshwater and seawater, and production costs are not too high. Spirulinaplatensis has a high nutrient content so it is used as a health food ingredient. Once the importance of the role of nutritional value of S. platensis for humans and some marine organisms, then the right culture medium to get the maximum nutritional content needs to be studied more deeply. This study aims to determine the effect of different doses of nutrition on the density of biomass cultivation of S. platensis microalgae with indoor and semi outdoor system. This research has been conducted from April to May 2018. The method by used the experimental method. The data obtained were analyzed statistically using simple linear regression equation. The results showed that the influence in the dose of nutris media guillard, S.platensis increased with the dose of nutrients as much as 0.60 ml either indoor or semi outdoor system. The relationship experienced between biomass with different doses of nutrients is a positive medium with both indoor and semi-outdoor systems, this can be seen from the results of simple linear regression test, namely r = 0.3443 for indoor system and r = 0.4492 for semi-outdoor system. As for the amount of biomass density S. platensis in the semi-outdoor system the amount of biomass density is higher than the indoor system.Key words: Microalga, Spirulina platensis, Sediment, Media Guillard
Inhibitory Powerof Lactic Acid Bacteria (BAL) from Cincaluk against pathogenic bacteria (Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus)and protease enzyme production Hutabarat, Povy; Nursyirwani, Nursyirwani; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Lactic acid bacteria (BAL) have the ability to inhibit the growth of pathogenic bacteria. BAL is bacterium that has an important role in the processof fermentation of organic materials. BAL can be found in traditional fishery products such as cincaluk (fermented shrimp). This study was conducted from February to April 2018. Test of isolate activity of BAL and protease enzyme was carried out at Marine Microbiology Laboratory, Faculty of Fisheries and Marine Scince, University of Riau.The study aimed was to examine the BAL activity of cincaluk against pathogenic bacteria and tested the protease enzyme produced by BAL from the cincaluk produced the community in Bengkalis and Tembilahan, Riau. This research used survey method where fresh cincaluk sample was used as abservation object in isolating the BAL and testing antagonism against three different pathogenic bacteria (Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus). The results of antagonism test showed that all bacterial isolated have ability to inhibit the growth of the three pathogenic bacteria. The highest inhibition zone P.aeruginosawas demonstrated by a GT isolate (11.0 mm), toward the highest inhibition zone against E. coliwas indicated by PB isolate (8.7 mm), and toward S. aureuswasdemonstrated by MB isolate (6.6 mm). All bacterial isolates produced protease enzyme indicated by clear zone around the colonies on skim milk medium. Keywords: BAL, Antagonism, Inhibition zone, Pathogens, Protease    
PRODUKSI DAN PENDAPATAN USAHATANI PADI DI DESA PUJO ASRI KECAMATAN TRIMURJO KABUPATEN LAMPUNG TENGAH Handayani, Siti Asih; Effendi, Irwan; Viantimala, Begem
Jurnal Ilmu-Ilmu Agribisnis Vol 5, No 4 (2017)
Publisher : Lampung University

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This research aimed to find out the productivity of hybrid rice and non-hybrid rice, factors affecting hybrid rice and non-hybrid rice production, the value or R/C and B/C ratio, and farm income difference between hybrid and non-hybrid rice.  This research was taken place purposively in Pujo Asri Village of Trimurjo Sub District for this village had sufficiently high rice productivity compared to other villages.  The samples of 60 respondents were drawn by simple random sampling technique, they were 30 hybrid and 30 non-hybrid rice farmers.  The data was analyzed statistically by Cobb-Douglas production function model, income analysis and income difference test.  The results of research showed that the mean productivity of Mapan 05 variety-hybrid rice was 15.02 ton/ha per year, and that of Ciherang variety-non hybrid rice was 13.36 ton/ha per year.  The affecting factors on hybrid rice production were land width (X1), labor (X2), and application level of panca usaha tani (five principles of agribusiness) (X3), while the factors affecting the non-hybrid rice production were land width (X1), and labor (X2).  The value of R/C ratio on hybrid rice farming was 1.55 and the non-hybrid rice was 1.56.  The B/C ratio was 0.48 in wet season and 0.44 in gadu season (transition from wet to dry).  The mean income of twice farming seasons per year of hybrid rice was IDR24,809,205.33 and that of non-hybrid rice was IDR23,001,337.25.  The result of different test analysis shows that there was no significant difference between the average income of hybrid and non-hybrid rice farming on 95 percent confidence level.Key words: farming income, hybrid rice, non-hybrid rice, productivity