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Pengaruh Media Induksi terhadap Multiplikasi Tu nas dan Pertumbuhan Planlet Pisang Rajabulu (AAB) dan Pisang Ta nduk (AAB) pada Berbagai Media Multiplikasi Kasutjianingati, ,; Poerwanto, Roedhy; Widodo, ,; Khumaida, Nurul; Efendi, Darda
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 3 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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 The aim of this research was to study the effects of explant from various induction media on multiplication and growth of cv Rajabulu  (AAB) and Tanduk (AAB) plantain.  This research was arranged in factorial complete randomized design with two factors. The first factor was two kinds of explant which came from induction media I1 (MS + BA 2 mg L-1 + IAA 3 mg L-1), and from induction media I2 (MS + BA 2 mg L-1 + IAA 3 mg L-1 + TDZ 0.09 mg L-1); the second factor was 4 kinds of multiplication media, i.e. MS0 (control/without PGR), MS + BA 0.20 mg L-1 + IAA 0.01 mg L-1 (M1), MS + BA 1 mg L-1 + IAA 0.25 mg L-1 (M2), MS + BA 2 mg L-1 + IAA 0.5 mg L-1 (M3).  The experiment results were the use of TDZ in the induction medium reduced the use of high cytokinin in the multiplication level. The use of Rajabulu explant that came from media I2 produced more shoots (4.3 shoots per explan) compared to explant from media I1 (3.2 shoots per explan). The use of multiplication media M3 and M2 produced the highest shoot number. The best shoot morphogenesis produced when the shoots after subculture in media with PGR (M3 or M2) to media MS0 (big shoot 3.1 and medium shoot 3.5). Tanduk plantain’s shoot was  responsive to cytokinin. The best treatment is I1-M3 with the highest number of shoots and the highest percentage of big and medium shoot is (33%) compared  to  other  treatments  (
Studi Pemberian Kalsium untuk Mengatasi Getah Kuning pada Buah Manggis (Garcinia mangostana L.) Dorly, ,; Wulandari, Indah; Tjitrosemito, Soekisman; Poerwanto, Roedhy; Efendi, Darda
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 1 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Gamboge is the main problem in mangosteen agribusiness because it is one of the major factors lowering fruit quality. Calcium is one of the important elements that strengthening cell wall; it is which was binding with the pectin as a middle lamella component. The objectives of the research were to study the effect of calcium application on the presence of gamboge spots, physical, and chemical properties of mangosteen fruit. Trial was conducted in a randomized complete block design with three replications in two consecutive years.  The calcium source was dolomite, applied in four different dosages, i.e. 0, 18, 24, and 34 ton ha-1 for the fi  rst year and 0, 12.5, 15, and 17.5 ton ha-1 for the second year. The results showed that calcium application raised soil pH and calcium content of the soil, exocarp and mangosteen leaves. Dolomite applications using 18 and 24 ton ha-1 in the first year and 17.5 ton ha-1 in the second year were effective to reduce gamboge spots on the outer part of fruit, however they were not effective to reduce gamboge in aril. Dolomite applications did not increase transversal diameter, fruit weight, total soluble solids, total titratable acids, and ratio of total soluble solids with total titratable acids of the fruits.   Keywords: aril, cell wall, dolomite, exocarp
REVIEW Rekayasa Genetika untuk Mengatasi Masalah-masalah Pascapanen Efendi, Darda
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 33, No 2 (2005): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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  Flavr Savr tomato is a transgenic tomato which is transformed using Polyglacturonase gene in antisense orientation. This is the first whole food product of biotechnology that reachs the market, but unfortunately it does not succeed.   Ethylene is thought to act as a natural triggering mechanism for fruit ripening and senescence.  Lowering the production of endogenous ethylene from fruit should delay fruit ripening and senescence. Several ways to control ethylene biosynthesis are: inactivation of the gene encoding ACC synthase, ACC oxidase, metabolism of SAM so that ACC is not produced, or metabolism of ACC before it can be converted to ethylene.  Effect of ethylene can also be blocked by blocking the perception of ethylene by specific tissues. Key words: Ethylene, ACC synthase, ACC oxidase, SAM hydrolase, genetic  transformation.
Efektifitas Aplikasi In-vitro Rizobakteri Sebagai Agen Antagonis Layu Fusarium pada Pisang Rajabulu/AAB di Rumah Kaca ., Kasutjianingati; Poerwanto, Roedhy; ., Widodo; Khumaida, Nurul; Efendi, Darda
Jurnal Hortikultura Indonesia Vol 2, No 1 (2011): Jurnal Hortikultura Indonesia
Publisher : Perhimpunan Hortikultura Indonesia

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Research was conducted on a banana cultivar of Rajabulu/AAB inside the green house. This research was aimed to study the effect of rhizobacteria and the ability of in vitro bacterization to prevent FOC. This study was conducted using split plot design. The main plot was rhizobacteria treatments (P. fluorescens ES-32, B. subtilis SB-3, a mix of both types and without bacteria), sub plots consist of in vitro (2 weeks and 1 week prior to application) and in vivo (during acclimatization) bacterization. All treatments were repeated 3 times, each consist of 7 polibags. The result of rhizobacteria treatment (B. subtilis SB3 or P. fluorecens ES32) was able to reduce the Disease Severity (Rhizome Discoloration) caused by Fusarium oxysporum f.sp cubense (FOC). The incorporation of mixed rhizobacteria provided significant effect suppressing the infestation of FOC. The application of mix bacteria was better than single treatment.Keywords: B. subtilis, P. fluorecens, rhizome discoloration, fusarial wilt, suppressing
The Use of Bee Wax, Chitosan and BAP to Prolong Shelflife of Mangosteen (Garcinia mangostana L.) Fruit efendi, darda; hermawati, heliyana
Jurnal Hortikultura Indonesia Vol 1, No 1 (2010): Jurnal Hortikultura Indonesia
Publisher : Perhimpunan Hortikultura Indonesia

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The objective of this research was to determine the effect of coating materials and BAP concentration on inhibition of  ripening process of mangosteen. The experiment used factorial completely randomized design with two factors, and three replications. Coating materials as the first factor consisted of control (without coating), bee wax 6%, and chitosan 2%. The second factor was concentration of Benzil Amino Purine (BAP), with 0, 5, 10, 15, and 20 ppm. Non destructive observations were weight loss, diameter decrease, peel and calyx color development. While, destructive observations were fruit hardness, total soluble solid (TSS), total titratable acidity (TTA) and opened ability. The result showed that bee wax was effective to inhibit weight loss. Interaction of bee wax and BAP 20 ppm inhibited peel and calyx color changes during storage.   Key word:  coating material, cytokinin, benzil amino purine, calyx, storage
A Review of Root Pruning to Regulate Citrus Growth Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda
Journal of Tropical Crop Science Vol 6 No 01 (2019): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Root is the important plant organ hiding below the soil that serves as plant supporting structure, water and nutrient supplier and assimilates storage. Root growth occurs in turn with shoot part, forming repeated cyclic pattern because of the photosynthate competition. Root growth regulation is one of the promising practices to boost the yield of fruit tree, especially citrus that is economically important and highly demanded fruit. Root pruning varied based on the scale of intensity and plant growth stage, including air root pruning, knife root pruning and modern pruning using root pruner machine mounted to a tractor. Root pruning to induce the growth of more fi brous root system and to correct root deformation at seedling stage have potentials to improve post-transplant growth. Besides, it has a potential to replace the use of chemical plant growth regulator for a more environmentally friendly practice. The pruning of root impeded the canopy growth, altered the plant water status leading to fl ower promotion. In addition, it is also a promising practice to overcome the alternate bearing on fruit tree, including citrus, through the suppressing of excessive growth and restricting the high fruit load during the ‘on year’ and allowing a better carbohydrate storage for the improvement of yield during the ‘off year’.
Analisis Genetik Sifat Ketahanan Melon (Cucumis melo L.) terhadap Virus Kuning Hermawan, Entit; ,, Sobir; Efendi, Darda
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 42, No 2 (2014): Jurnal agronomi indonesia
Publisher : Bogor Agricultural University

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ABSTRACTResistance to yellow virus (YV) is an important breeding trait in melon. However information regarding genetic inheritance pattern of the character are limited. This study aimed to provide information on genetic control for resistance to YV in melon caused by Tomato Yellow Leaf Curl Virus (TYLCV). Twenty genotypes from three major melon groups (dudaim, cantaloupe, and inodorous) were evaluated using controlled inoculation method. The results revealed that one line, MEV1 from the dudaim group, showed high resistance to YV; while other lines belong to cantaloupe and indororus indicated as highly susceptible lines. Screening of the F1 from crossing between resistant and susceptible parents resulted in resistant F1 after inoculation and planted in endemic location. Subsequently, evaluation on F2 population revealed a non-normal distribution for disease severity score, indicating that resistance to YV in melon was controlled by major genes. Chi-square (χ2) test resulted in 13:3 ratio and indicated that the resistance to YV was controlled by 2 genes pair with dominant and epistasis recessive actions.Keywords: dominant and epstasis recessive action, major gene, Bemicia tabaci, Begomovirus
DEHIDRASI DAN PEMBEKUAN JARINGAN APEKS TEBU UNTUK PENYIMPANAN JANGKA PANJANG ROOSTIKA, IKA; DEWI LIMA WATI, RARA PUSPITA; EFENDI, DARDA
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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ABSTRAKTebu (Saccharum officinarum L.) merupakan tanaman yangdiperbanyak secara vegetatif. Kriopreservasi merupakan metode yangpaling sesuai untuk penyimpanan jangka panjang bagi tanaman yangdiperbanyak secara vegetatif. Dehidrasi dan pembekuan jaringan merupa-kan tahapan paling kritis yang menentukan keberhasilan kriopreservasi.Tujuan penelitian adalah untuk memperoleh durasi dehidrasi yang optimaldan metode pembekuan jaringan apeks tebu. Penelitian dilakukan diLaboratorium Kultur Jaringan, Kelompok Peneliti Biologi Sel danJaringan, Balai Penelitian dan Pengembangan Bioteknologi dan Sumber-daya Genetik Pertanian, Bogor pada Mei 2013 sampai Februari 2014.Untuk optimasi metode dehidrasi, apeks direndam dalam larutan PVS2(MS + gliserol 30% + etilen glikol 15% + dimetil sulfoksida 15% +sukrosa 0,4 M) selama 10, 20, 30, dan 40 menit. Untuk optimasi metodepembekuan, diujikan kombinasi perlakuan prakultur (dengan sukrosa 0;0,1; dan 0,3 M selama 5 hari) dan pemuatan dalam larutan LS (MS +gliserol 2 M + sukrosa 0,4 M) selama 0, 10, 20, dan 30 menit sebelumtahapan dehidrasi dan pembekuan jaringan di dalam nitrogen cair (-196 o C). Hasil penelitian menunjukkan durasi dehidrasi jaringan yangterbaik adalah 30 menit dalam larutan PVS2. Kombinasi perlakuanprakultur dengan sukrosa 0,3 M dan pemuatan dengan larutan LS selama10 menit merupakan metode terbaik untuk pembekuan jaringan. Persentasetumbuh sebelum dan setelah pembekuan dalam nitrogen cair berturut-turutadalah 100 dan 40%. Setelah kriopreservasi, biakan mampu tumbuhdengan tingkat multiplikasi tunas sekitar 10 tunas/eksplan. Metode yangdiperoleh pada penelitian ini berpeluang diterapkan untuk penyimpananplasma nutfah tebu dalam jangka panjang secara kriopreservasi daneliminasi patogen obligat secara krioterapi.Kata kunci: Saccharum officinarum L., apeks, dehidrasi, pembekuan,nitrogen cairABSTRACTSugarcane (Saccharum officinarum L.) is vegetatively propagatedplant. Cryopreservation is the most suitable method for long-termpreservation of vegetatively propagated plant. Dehydration and freezingare critical steps of successful cryopreservation so that it should beoptimized. The research aimed to obtain the optimal duration ofdehydration and freezing method of sugarcane apex tissues. Theexperiments were conducted at Tissue Culture Laboratory, Plant CellTissue Biology Group, Indonesian Center for Agricultural Biotechnologyand  Genetic  Resources  Research  and  Development  on  May2013−February 2014. To optimize dehydration method, the tissues wereexposured in PVS2 solution (MS + 30% glycerol + 15% ethylene glycol +15% dimethyl sulphoxide + 0.4 M sucrose) for 10, 20, 30, and 40 minutes.To optimize freezing method, the combined treatment of preculture withsucrose (0, 0.1, dan 0.3 M) for 5 days and loading in LS solution (MS + 2M glycerol + 0.4 M sucrose) for 0, 10, 20, dan 30 minutes) were testedbefore dehydration for 30 minutes and freezing in liquid nitrogen (-196 o C).The best duration of dehydration was 30 minutes. The combined treatmentof preculture on 0.3 M sucrose and loading for 10 minutes was the bestmethod for tissues freezing. Percentage of regrowth before and afterfreezing in liquid nitrogen was 100 and 40% respectively. Aftercryopreservation, the cultures could grow with high shoot multiplicationrate about 10 shoots/explant. The method resulted in this study can beapplied for long-term storage of sugarcane germplasms by cryopreser-vation and (elimination of obligate pathogens by cryotherapy.Keywords: Saccharum officinarum L., apex, dehydration, freezing, liquidnitrogen.
Perubahan Warna Kulit Buah Tiga Varietas Jeruk Keprok dengan Perlakuan Degreening dan Suhu Penyimpanan Muthmainnah, Hanifah; Poerwanto, Roedhy; Efendi, Darda
Jurnal Hortikultura Indonesia Vol 5, No 1 (2014): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.5.1.10-20

Abstract

ABSTRACTColor is  the main  quality  that determines  the level of demand  for  citrus. Consumer prefersorange  citrus,  where  as  green  citrus  have  high productivity  in  Indonesia.  It  causes  local  citrus cannot compete with imported citrus.  One way  that  can  make local citrus compete  with  import citrus  is by  improving of colour quality through  degreening.  This research was conducted to study the effect of degreening and storage temperature on color changes of tangerine peel. Research was conducted from June until  July 2013 at the Laboratory of  Center for Tropical Horticulture Studies, IPB. This research used a randomized complete factorial design group  2  factors with  3 replications. The first factor is degreening  temperature  (180 C  and  room temperature),  second  factor is storage temperature (180 C  and room temperature). The results showed that interaction  of  degreening and storage temperature significantly  affected  skin  coloration.  Degreening  treatment 180 C  and  room temperature storage on all three varieties of tangerines have the highest Citrus Colour  Index  (CCI) value  at  15  HSP.  The  higher  value of Citrus  Colour  Index,  the  higher  orange  skin  colorationproduced.Key words : degreening temperature, orange, storage temperature, value of Citrus Color Index. ABSTRAKWarna  merupakan  kualitas  utama  yang  menentukan  tingkat  permintaan konsumen  terhadap buah jeruk. Konsumen lebih menyukai jeruk berwarna jingga, padahal di Indonesia jeruk yang tinggi produktivitasnya  adalah  jeruk berwarna  hijau.  Hal  ini  menyebabkan  jeruk  lokal  kalah  bersaing dengan jeruk impor. Salah satu cara supaya jeruk lokal dapat bersaing dengan jeruk impor adalah dengan  melakukan  degreening.  Penelitian  ini  dilakukan  untuk mempelajari  efek  degreening  dan suhu penyimpanan terhadap perubahan warna kulit jeruk keprok. Penelitian dilaksanakan dari bulan Juni  hingga  Juli 2013  di  Laboratorium  Pusat  Kajian  Hortikultura  Tropika,  IPB.  Penelitian ini menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) Faktorial dengan dua faktor dan tigaulangan. Faktor pertama adalah suhu  degreening (180C dan suhu ruang), faktor kedua  adalah suhu simpan (180C dan suhu ruang). Hasil penelitian menunjukkan interaksi suhu  degreening  dan suhu simpan nyata pada perubahan warna. Perlakuan degreening suhu 180C dan penyimpanan suhu ruang pada ketiga varietas jeruk keprok memiliki nilai  Citrus Colour  Index  (CCI)  tertinggi dibandingkan perlakuan lainnya pada 15 HSP. Semakin tinggi nilai CCI, maka warna kulit jeruk yang dihasilkan semakin jingga.Kata kunci: jingga, nilai Citrus Color Index, suhu degreening, suhu penyimpanan.
Morfogenesis Eksplan Keping Biji dari Tiga Klon Manggis (Garcinia mangostana L.) Pada Tiga Jenis Media Dasar Joni, Yosi Zendra; Efendi, Darda; Tambunan, Ika Roostika
Jurnal Hortikultura Vol 24, No 2 (2014): Juni 2014
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v24n2.2014.p94-101

Abstract

Sistem regenerasi manggis secara in vitro merupakan metode alternatif yang mendukung upaya produksi benih secara masal dan pemuliaan tanaman manggis secara bioteknologi. Dalam kultur in vitro, jenis klon dan media dasar sangat menentukan pertumbuhan biakan manggis. Penelitian ini bertujuan untuk mempelajari respons in vitro (morfogenesis) tiga klon manggis yang dikulturkan pada tiga jenis media dasar. Penelitian dilaksanakan di Laboratorium Kultur Jaringan, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian (BB Biogen) dari bulan Januari sampai Agustus 2013. Percobaan disusun secara faktorial dalam lingkungan rancangan acak lengkap. Faktor pertama yaitu eksplan yang berasal dari tiga klon manggis (Leuwiliang, Wanayasa, dan Puspahiang). Faktor kedua adalah tiga jenis media dasar (MS, WPM, dan B5). Setiap media diperkaya dengan gula 30 g/l, phytagel 2,5 g/l, glutamin 300 mg/l, dan 6-benzyldenine (BA) 5 mg/l. Setiap perlakuan terdiri atas 25 ulangan (botol). Dalam setiap botol terdapat tiga irisan melintang biji manggis yang berasal dari satu biji. Hasil penelitian menunjukkan bahwa tidak terdapat interaksi antara jenis klon dengan jenis media dasar untuk peubah jumlah tunas, tinggi tanaman, jumlah daun/tunas, dan jumlah daun total. Terdapat interaksi antara jenis klon dengan jenis media dasar, yaitu untuk peubah jumlah nodul. Menariknya, penggunaan media MS menyebabkan jumlah tunas, jumlah daun total, dan jumlah nodul yang terbanyak. Tinggi tunas tertinggi diperoleh dari penggunaan media WPM dan B5. Selain itu, morfogenesis klon Wanayasa dan Puspahiang lebih baik daripada klon Leuwiliang