Dedy Duryadi Solihin
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Variasi Molekuler Gen Reseptor Melanokortin-4 pada Monyet Ekor Panjang Arta Putra, I Gusti Agung; Sajuthi, Dondin; Duryadi Solihin, Dedy; Roro Dyah Perwitasari, Raden
Jurnal Veteriner Vol 11, No 3 (2010)
Publisher : Jurnal Veteriner

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Abstract

Melanocortin-4 receptor (MC4R) is one of G protein-coupled receptors that plays an important role inregulation of energy homeostasis. MC4R mutations constitute the most common cause of human obesity.The study was conducted in order to investigate the variation of MC4R gene in Macaca fascicularis and itsassociation with obesity as a model of human obesity. Forty eight adult male macaques from Bali (Ubudand Uluwatu), East Java (Alas Purwo and Baluran), and Sumatera island (Palembang) were used in thisresearch. The animals had been anaesthetized using ketamine (10 mg/kg body weight) and xylazine (2mg/kg body weight) before collecting blood samples and phenotypic data (weight, crown rump length. Bloodsamples were used as source of DNA. To determine MC4R variation, coding region of this gene wasamplified and sequenced. The results showed that 20 variations sites were identified and 13 of them werenon-synonymous. Among the non-synonymous mutations, five mutations were only found in obese macaque;two mutations were found both in obese and non-obese macaque; and six mutations were only found in nonobesemacaque .
Genetic Diversity and Phylogenetic Relationship of Kryptoterus limpok and Kryptopterus apogon from Kampar and Indragiri River Based on Cytochrome b Gene Elvyra, Roza; Duryadi Solihin, Dedy; Affandi, Ridwan; Junior, Zairin; Yus, Yusnarti
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol 16, No 1 (2009): Juni 2009
Publisher : Institut Pertanian Bogor

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Abstract

The utility of cytochrome b gene as a molecular marker to obtain genetic diversity and phylogenetic relationship among Kryptopterus spp. consist of Kryptopterus limpok and Kryptopterus apogon from Riau Province has been studied. The universal primers of (F) L14841 and (R) H15149 were used to amplify the cytochrome b gene. The results of multiple allignment were 159 nt (coding 53 amino acids). Kryptopterus limpok from Kampar and Indragiri River form a phylogeny cluster at 80% bootstrap value. Kryptopterus apogon from Kampar and Indragiri River form a phylogeny cluster at 99% bootstrap value.Keywords : cytochrome b, Kryptopterus limpok, Kryptoperus apogon.
Genetic Diversity and Phylogenetic Relationship of Kryptoterus limpok and Kryptopterus apogon from Kampar and Indragiri River Based on Cytochrome b Gene Elvyra, Roza; Duryadi Solihin, Dedy; Affandi, Ridwan; Junior, Zairin; Yus, Yusnarti
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol 16, No 1 (2009): Juni 2009
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

The utility of cytochrome b gene as a molecular marker to obtain genetic diversity and phylogenetic relationship among Kryptopterus spp. consist of Kryptopterus limpok and Kryptopterus apogon from Riau Province has been studied. The universal primers of (F) L14841 and (R) H15149 were used to amplify the cytochrome b gene. The results of multiple allignment were 159 nt (coding 53 amino acids). Kryptopterus limpok from Kampar and Indragiri River form a phylogeny cluster at 80% bootstrap value. Kryptopterus apogon from Kampar and Indragiri River form a phylogeny cluster at 99% bootstrap value.Keywords : cytochrome b, Kryptopterus limpok, Kryptoperus apogon.