DORLY DORLY
Departemen Biologi, FMIPA, Institut Pertanian Bogor, Kampus Dramaga, Bogor 16680, Indonesia

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Secretory Duct Structure and Phytochemistry Compounds of Yellow Latex in Mangosteen Fruit DORLY, DORLY; TJITROSEMITO, SOEKISMAN; POERWANTO, ROEDHY; JULIARNI, JULIARNI
HAYATI Journal of Biosciences Vol 15, No 3 (2008): September 2008
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Yellow latex is the main problem in mangosteen agribusiness, because it is one factor lowering the fruit quality. The structure of yellow latex secretory ducts in the flower and fruit as well as in the root, stem and leaf of mangosteen (Garcinia mangostana L.) seedling and the qualitative phytochemistry of yellow latex were studied. The ducts were branched, canal-like type. They were found in the exocarp, mesocarp, endocarp, aril of the fruit, flower, stem, and leaf. In the fruit, the biggest diameter of the secretory ducts was found in the endocarp. There were continuous secretory ducts from fruit stalk to the fruit. Ultrastructural observation showed that the ducts surrounded by specific epithelial cells, which were living cells containing dense cytoplasm with plastid, mitochondria and golgi apparatus organelles. The qualitative test indicated that the yellow latex collected from stem bark, outer part of fruit, young fruit pericarp, mature aril and young aril contained terpenoid, flavonoid and tannin, but not alkaloid, saponin and steroid, except in the young aril containing the steroid. Key words: secretory ducts, yellow latex, endocarp, aril, epithelial cells
Keanekaragaman Serangga pada Bunga Tomat (Lycopersicon esculentum Mill.) di Lahan Pertanian Organik Fajarwati, Mosi Retnani; Atmowidi, Tri; Dorly, Dorly
Jurnal Entomologi Indonesia Vol 6, No 2 (2009): September
Publisher : Perhimpunan Entomologi Indonesia

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Abstract

Study on the diversity of insect visitors on tomato flowers were conducted at Bina Sarana Bakti (BSB) organic farm, Cisarua, West Java. Diversity of insects were observed by observational method, an 10 m2 area of tomato plants in the morning, mid-day, and afternoon. Observations were conducted during 20 days. Diversity of visiting insects were analyzed by Shannon diversity and its evenness. Similarity of visiting insects between time of observations were analyzed by Jaccard similarity index. In organic farm, insect visitors of tomato plants consist of six orders belonging to eight species. Insect visitors of tomato flowers were dominated by Thrips sp. (Thysanoptera) and Hylaeus sp. (Hymenoptera). Hylaeus sp. was a main pollinator of tomato plants. Diversity of insect visitors in the morning and mid-day was higher than in the afternoon. Evenness of insect visitors of tomato flowers between observation times were relatively equal.
KARAKTERISTIK ANATOMI DAUN DARI SEPULUH SPESIES Hoya SUKULEN SERTA ANALISIS HUBUNGAN KEKERABATANNYA Hafiz, Putra; Dorly, Dorly; Rahayu, Sri
Buletin Kebun Raya Vol 16, No 1 (2013): Buletin Kebun Raya Vol. 16 (1) Januari 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Hoya has two types of leaves, succulent and non succulent. Succulent plant adapted well in extreme conditions, especially during dry period. Anatomical characters can be used to identify, classify, and determine plant relationship. The aims of this research were to identify anatomical character of succulent Hoya’s leaf and to determine their relationship. Ten examined species Hoya spp. (H. diversifolia, H. latifolia, H. dolichosparte, H. bilobata, H. lacunosa, H. verticillata, H. purpureofusca, H. kuhlii, H. oblanceolata, and H. micrantha) showed anatomical similarity in their epidermal layer, hipodermal, mesophyl, and the absence of sponge tissue at mesophyl. However, there are diversities among the species. Based on paradermal section of the leaf,  two types of stomata were only found at the abaxial side, individual stomata and both individual and stomatal cluster. Hoya diversifolia has the smallest stomatal size, while Hoya latifolia has the biggest. Hoya lacunosa has the lowest stomatal density, while Hoya bilobata has the highest. Hoya diversifolia, Hoya lacunosa, and Hoya oblanceolata have the lowest stomatal index, while Hoya bilobata has the highest. Observation on transversal section showed that Hoya purpureofusca has the lowest leaf thickness, while Hoya kuhlii has the highest. The hierarchical cluster analysis based on anatomical leaf characters showed different patterns of relationship which diversed as three groups at relationship scale 15.
KERAGAMAN DAN ANALISIS KEKERABATAN Hoya spp. BERTIPE DAUN NON SUKULEN BERDASARKAN KARAKTER ANATOMI DAUN Hakim, Aldi Rahman; Dorly, Dorly; Rahayu, Sri
Buletin Kebun Raya Vol 16, No 1 (2013): Buletin Kebun Raya Vol. 16 (1) Januari 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Hoya spp. (Apocynaceae: Asclepiadoideae) has differences in leaf type, there are succulent and non succulent leaf types.  Anatomical structure of Hoya spp. leaf has not been widely studied, especially for the non succulent type. The aims of this research were to explore the diversity and relationship of non succulent Hoya, based on leaf anatomical characters represented by eight species i.e. H. bandaensis, H. campanulata, H. chlorantha, H. cilliata, H. coriacea, H. coronaria, H. densifolia and H. multiflora. Hoya leaf anatomical characters were observed on the paradermal and transversal section and analyzed by using IBM SPSS version 19 sotfware for cluster analysis. According to the paradermal observation, stomata were present at lower surface (hypostomatic) for all species, and amphistomatic (both surface) for H. densifolia.  The type of stomata is cyclocytic for all species. Clustered stomata were found in H. coriacea.  Observation on transversal section showed that all of species has the normal structure i.e cuticula, upper and lower epidermis, palisade parenchyma and spongy parenchyma with the variation in the layer thickness.  The cluster analysis resulted four groups at distance scale 19. Each group has specific characters. The first group has trichomes on both sides of the leaf surface. The second group has amphistomatic stomatal. The third group has thin leaves. The fourth group has the widest stomata.
Secretory Structure and Histochemistry Tests of Asteraceae Family Members of Medicinal Plants in Walat Mountain Educational Forest Dorly, Dorly; RR. Syafira Nidyasari, Bimo Adi Wiryo, Ismi Nurfaizah,
Prosiding Seminar Biologi Vol 12, No 1 (2015): Seminar Nasional XII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

The diversity of Walat Mountain Educational Forest is not only fauna but also very rich of various kinds of flora such as several kinds of medicinal plants.  This research was aimed study the variety of secretory structure and histochemistry of three kinds of medicinal plants found in of Walat Mountain Educational Forest.  Plant materials used  were billy-goat weed (Ageratum conyzoides), balakacida (Mikania cordata), and  monyenyen (Erigeron linifolius).The medicinal plant leaves were made into paradermal and transversal sections, then secretory structures were observed,  and the histochemistry of terpenoid, alkaloid, phenol and oil compounds was tested. The results of this research on adaxial and abaxial billy-goat weed leaves were found multicellular glandular trichome and peltate type glandular trichome. Besides, oil drops were also found on billy-goat weed palisade tissue.  On adaxial and abaxial bakalacida leaves were found multicellular glandular trichome type 1 and type 2, andpeltate type glandular trichome.  Moreover on adaxial and abaxial monyenyen leaves were found multicellular glandular trichome type 1 and type 2.  The results of histochemical test on billy-goat weed were the multicellular glandular  trichome did not contain terpenoid, alkaloid, phenol, and oil compounds, while on peltate type glandular trichome was positive to terpenoid.  Both types of multicellular glandular  trichomes on balalakacida plant were positive to terpenoid, alkaloid, and oil compounds, except phenol.  Whereas peltate type glandular trchome was positive to alkaloid, phenol, and oil compounds, except terpenoid.  Histochemical test results on both types of multicellular glandular trichomes of monyenyen plant were positive to terpenoid and oil compounds.Keywords: secretory structure,  histochemical tests, glandular trichome
KARAKTERISTIK ANATOMI DAUN DARI SEPULUH SPESIES Hoya SUKULEN SERTA ANALISIS HUBUNGAN KEKERABATANNYA Hafiz, Putra; Dorly, Dorly; Rahayu, Sri
Buletin Kebun Raya Vol 16, No 1 (2013): Buletin Kebun Raya Vol. 16 (1) January 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Hoya has two types of leaves, succulent and non succulent. Succulent plant adapted well in extreme conditions, especially during dry period. Anatomical characters can be used to identify, classify, and determine plant relationship. The aims of this research were to identify anatomical character of succulent Hoya’s leaf and to determine their relationship. Ten examined species Hoya spp. (H. diversifolia, H. latifolia, H. dolichosparte, H. bilobata, H. lacunosa, H. verticillata, H. purpureofusca, H. kuhlii, H. oblanceolata, and H. micrantha) showed anatomical similarity in their epidermal layer, hipodermal, mesophyl, and the absence of sponge tissue at mesophyl. However, there are diversities among the species. Based on paradermal section of the leaf,  two types of stomata were only found at the abaxial side, individual stomata and both individual and stomatal cluster. Hoya diversifolia has the smallest stomatal size, while Hoya latifolia has the biggest. Hoya lacunosa has the lowest stomatal density, while Hoya bilobata has the highest. Hoya diversifolia, Hoya lacunosa, and Hoya oblanceolata have the lowest stomatal index, while Hoya bilobata has the highest. Observation on transversal section showed that Hoya purpureofusca has the lowest leaf thickness, while Hoya kuhlii has the highest. The hierarchical cluster analysis based on anatomical leaf characters showed different patterns of relationship which diversed as three groups at relationship scale 15.
KERAGAMAN DAN ANALISIS KEKERABATAN Hoya spp. BERTIPE DAUN NON SUKULEN BERDASARKAN KARAKTER ANATOMI DAUN Hakim, Aldi Rahman; Dorly, Dorly; Rahayu, Sri
Buletin Kebun Raya Vol 16, No 1 (2013): Buletin Kebun Raya Vol. 16 (1) January 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Original Source | Check in Google Scholar

Abstract

Hoya spp. (Apocynaceae: Asclepiadoideae) has differences in leaf type, there are succulent and non succulent leaf types.  Anatomical structure of Hoya spp. leaf has not been widely studied, especially for the non succulent type. The aims of this research were to explore the diversity and relationship of non succulent Hoya, based on leaf anatomical characters represented by eight species i.e. H. bandaensis, H. campanulata, H. chlorantha, H. cilliata, H. coriacea, H. coronaria, H. densifolia and H. multiflora. Hoya leaf anatomical characters were observed on the paradermal and transversal section and analyzed by using IBM SPSS version 19 sotfware for cluster analysis. According to the paradermal observation, stomata were present at lower surface (hypostomatic) for all species, and amphistomatic (both surface) for H. densifolia.  The type of stomata is cyclocytic for all species. Clustered stomata were found in H. coriacea.  Observation on transversal section showed that all of species has the normal structure i.e cuticula, upper and lower epidermis, palisade parenchyma and spongy parenchyma with the variation in the layer thickness.  The cluster analysis resulted four groups at distance scale 19. Each group has specific characters. The first group has trichomes on both sides of the leaf surface. The second group has amphistomatic stomatal. The third group has thin leaves. The fourth group has the widest stomata.
DISTRIBUSI KAPULASAN (NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.) DI PULAU JAWA DAN HUBUNGAN KEKERABATAN MORFOLOGINYA Djuita, Nina Ratna; Hartana, Alex; Chikmawati, Tatik; Dorly, Dorly
Floribunda Vol 5, No 4 (2016)
Publisher : PTTI

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Abstract

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribution of pulasan [Nephelium  ramboutan-ake (Labill.) Leenh] in Java and Relationship of Their Morphology. Floribunda 5(4): 129–138. — Distribution of pulasan in Java is not known yet. The purposes of this study were to provide information about the locations of pulasan trees in Java, and to analyze relationship of this species based on morphological characters. Pulasan exploration was carried out in four provinces in Java encompassed West Java, Central Java, East Java, and Yogyakarta Special Region. Morphological characteristics of pulasan were observed, then the relationships were analyze using NTSys pc 2.02 program.  Result showed that most of pulasan located in West Java, especially in the area of Bogor.  Based on the dendrogram, it was known that the use of ve-getative dan generative characters could distinguish males and hermaphrodite pulasan trees, while the use of vegetative characters only, could not differentiate the type of pulasan trees. Pulasan trees from different areas can be grouped in the same cluster because of the similarity of their morphological traits.Keywords: Kapulasan, morphological characters, dendrogram, Sapindaceae. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribusi Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Pulau Jawa dan Hubungan Kekerabatan Morfologinya. Floribunda 5(4): 129–138. — Persebaran kapulasan di Pulau Jawa belum diketahui dengan pasti.  Penelitian ini bertujuan memberikan informasi tentang lokasi-lokasi yang masih mempunyai pohon kapulasan di Pulau Jawa, dan menganalisis hubungan kekerabatannya berdasarkan ciri morfologi. Eksplorasi kapulasan dilakukan di empat provinsi di Pulau Jawa yaitu Jawa Barat, Jawa Tengah, Jawa Timur, dan Daerah Istimewa Yogyakarta. Tanaman hasil eksplorasi diamati ciri morfologinya, kemudian dianalisis hubungan kekerabatannya dengan menggunakan program NTSys pc 2.02.  Hasil penelitian menunjukkan bahwa sebagian besar kapulasan terdapat di Jawa Barat terutama di daerah Bogor. Berdasarkan dendrogram yang dihasilkan, diketahui bahwa penggunaan ciri  vegetatif dan generatif dapat membedakan kapulasan jantan dan hermafrodit, sedangkan penggunaan ciri  vegetatif saja, tidak dapat membedakan kedua tipe tanaman tersebut. Tanaman kapulasan dari daerah yang berbeda dapat mengelompok dalam satu grup yang sama karena adanya keserupaan dalam ciri-ciri morfologinya.Kata kunci: Kapulasan, karakter morfologi, dendrogram, Sapindaceae. 
Anatomi Daun Beberapa Talas Liar (Colocasia esculenta (L.) Schott dari Kabupaten Bogor Dorly, Dorly; Sulistyaningsih, Yohana C
Caraka Tani: Journal of Sustainable Agriculture Vol 22, No 1 (2007): March
Publisher : Universitas Sebelas Maret

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Abstract

Leaf anatomy characters are commonly stable, however some of them can change because of environmental influence. Identification of those characters are necessary to be conducted by observing anatomical structure of the plants grown out side of the natural habitat. The objective of this research is to examine the stability of leaf anatomy characters of wild taro on their natural habitat and after planted in the dry field. Leaf anatomical observation was conducted by preparing paradermal and transversal sections. The result indicated that leaf anatomical character was the number of palisade layers of Ciju-2 and Jonggol grown out side their natural habitat was stable. Leaf anatomical characters of Ciju-2 collection were palisade thickness, sponge thickness, leaf thickness, stoma density on abaxial surface changed after planted on dry field. Whereas on Jonggol collection significant difference were found on papil height on abaxial surface, stomatal index on abaxial surface, and palisade thickness on dry field and natural habitat.
Secretory Duct Structure and Phytochemistry Compounds of Yellow Latex in Mangosteen Fruit DORLY, DORLY; TJITROSEMITO, SOEKISMAN; POERWANTO, ROEDHY; JULIARNI, JULIARNI
HAYATI Journal of Biosciences Vol 15, No 3 (2008): September 2008
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Yellow latex is the main problem in mangosteen agribusiness, because it is one factor lowering the fruit quality. The structure of yellow latex secretory ducts in the flower and fruit as well as in the root, stem and leaf of mangosteen (Garcinia mangostana L.) seedling and the qualitative phytochemistry of yellow latex were studied. The ducts were branched, canal-like type. They were found in the exocarp, mesocarp, endocarp, aril of the fruit, flower, stem, and leaf. In the fruit, the biggest diameter of the secretory ducts was found in the endocarp. There were continuous secretory ducts from fruit stalk to the fruit. Ultrastructural observation showed that the ducts surrounded by specific epithelial cells, which were living cells containing dense cytoplasm with plastid, mitochondria and golgi apparatus organelles. The qualitative test indicated that the yellow latex collected from stem bark, outer part of fruit, young fruit pericarp, mature aril and young aril contained terpenoid, flavonoid and tannin, but not alkaloid, saponin and steroid, except in the young aril containing the steroid. Key words: secretory ducts, yellow latex, endocarp, aril, epithelial cells