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Number and Quality of Oocytes Collected from Heterotopic Autografted Mice Ovary after PMSG Induction NURBARIAH, .; DJUWITA, ITA; MOHAMAD, KUSDIANTORO; SUPRIATNA, IMAN
HAYATI Journal of Biosciences Vol 18, No 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P < 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.
Aktivitas NADH- tetrazolium reductase sel sel trofoblas pada blastosis yang mengalami hatching dan gagal hatching Helmita, Roza; Djuwita, Ita; Purwantara, Bambang; Winarto, Adi
Jurnal Anatomi Indonesia Vol 1, No 2 (2007)
Publisher : Jurnal Anatomi Indonesia

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Abstract

Implantation is themost critical stage in the establishment of pregnancy. Inmammals, it has been estimated thatbetween 25%and 60%of conceptuses are lost before or at the time of implantation. The objectives of this studywere to investigate the activity of themitochondrial NADH-tetrazoliumreductase, the outgrowth and differentiationof trophoblast cells in in vitro culture of hatched and non hatched blastocyst. Blastocysts were collectedfrom mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatchingwithin 24 hours, 48 hours and non hatching. Embryos were cultured in DMEMmediumin 5%CO2 incubator at37°C for 10 days.The trophoblastsmonolayer were processed forGimsa staining and histochemistry analysisof NADH-tetrazolium reductase activity. The outgrowth of trophoblast cells were measured using eyespiecemicrometer. The results showed that the activity of NADH-tetrazolium reductase of 24 h and 48 h hatchedblastocysts showed higher intensity than the non hatched blastocysts (P&lt;0,05). The trophoblast outgrowthdiameter of 24 h hatched blastocystwas significantly higher than the non hatched blastocyst, but not signifcantlydifferent with the 48 h hatched.Morphology examination using light microscope showed that the trophoblastmonolayer of 24 h hatched blastocyst differentiated into cytotrophoblast, syncytioptrophoblast andspongiotrophoblast after 5 days of in vitro cultured. In conclusion, in in vitro sudy the failure of blastocystsimplantationwas due to the impairment ofNADHdehydrogenase activity in complex Imitochondria and the failureof outgrowth and differentiation of the trofoblast cells.
Development of Domestic Cat Embryo Produced by Preserved Sperms ERIANI, KARTINI; BOEDIONO, ARIEF; DJUWITA, ITA; SUMARSONO, SONY HERU; AL-AZHAR, AL-AZHAR
HAYATI Journal of Biosciences Vol 15, No 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 oC, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 oC for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (P < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 oC in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos. Key words: gamet, preservation, in vitro fertilization
In Vitro Fertilization and Embryo Development DJUWITA, ITA; BOEDIONO, ARIEF; AGUNGPRIYONO, SRIHADI; SUPRIATNA, IMAN
HAYATI Journal of Biosciences Vol 12, No 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Experiments were conducted on the morphology, fertilization and embryo development rate of vitrified ovine oocytes matured in vitro. Three vitrification solutions were used for vitrification. PBS supplemented with 1% BSA, 30% ethylene glycol was added by one of three different sucrose concentrations, 1.00 M (VS1), 0.50 M (VS2), and 0.25 M (VS3). The results showed that the percentages of normal vitrified oocytes after warming were 78 and 63% in VS1 and VS2, respectively, which was significantly higher as compared for VS3. The fertilization rates were 59 and 66% in VS1 and VS2, respectively, which were also significantly higher as compared with VS3 (35%). Zygote viability after 18 h was 57; 43; and 40%, for VS1,VS2, and VS3, respectively, which was not significantly different. The incidence of polyspermic penetration increased with increasing sucrose concentration, i.e 23, 11, and 9% in VS1, VS2, and VS3, respectively, as compared with unvitrified oocytes (4%). The cleavage rate of vitrified oocytes in VS1 was 13.2% which was significantly lower (p
Allotransplantasi Testis Mencit Muda sebagai Upaya Preservasi Gonad In Vivo Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Fahrudin, Mokhamad; Djuwita, Ita; Agungpriyono, Srihadi
Jurnal Ilmu Pertanian Indonesia Vol 12, No 1 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Cancer disease are not detected only at adult but also at young age. One therapy for cancer diseases is chemotherapy and radiation, that give a side effect of infertility in the gonad, therefore, it is necessary to preserve the gonad. Sperm collection from adult is easy but not from the young patients.Keyword: transpalantation, testis, mencit muda, spermatogenesis
TAHU MENGHAMBAT KEHILANGAN TULANG LUMBAR TIKUS BETINA OVARIEKTOMI [Tofu Attenuates Lumbar Bone Loss of Ovariectomized Female Rats] Nurdin, Samsu Udayana; Muchtadi, Deddy; Djuwita, Ita; Pawiroharsono, Suyanto
Jurnal Teknologi Dan Industri Pangan Vol 13, No 3 (2002): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

TAHU MENGHAMBAT KEHILANGAN TULANG LUMBAR TIKUS BETINA OVARIEKTOMI   [Tofu Attenuates Lumbar Bone Loss of Ovariectomized Female Rats] Samsu Udayana Nurdin 1) , Deddy Muchtadi 2) , Ita Djuwita 3) , Suyanto Pawiroharsono 4) 1) Department of Agricultural Product Technology, Lampung University, Jln. Sumantri Brojonegoro No. 1Bandar Lampung, 35145;  2) Department of Food Technology and Human Nutrition, Bogor Agriculture University, Jln. Lingkar Kampus, Dramaga Bogor; 16680; 3) Laboratory of Embriology, Veteriner Faculty, Bogor Agriculture University, Jln. Agatis, Dramaga Bogor; 16680; 4) Agency for the Assesment and Application of Technology, Jln. MH. Thamrin no. 8, Jakarta 10340. ABSTRACT   The objectives of this research were to examine the efeects of feed containing soybean tofu and tempeh on lumbar bone density and mass of ovariectomized female rats. Twenty four 17 weeks-old Sprague-Dawley rats were randomly assigned to four group, i.e.: (1) non-ovariectomized rats fed casein based diet (NonOvx), (2) ovariectomized rats fed casein based diet (OvxC), (3) ovariectomized rats fed diet containing soybean tofu (OvxH), and (4) ovariectomized rats fed diet containing soybean tempeh (OvxT); in three block based on their body weight.  The result show that body weight gram of ovariectomized rats was greater than nonovariectomized.  Ovariectomy caused atrophy of the uterus, and resulted in higher serum calcium level.  The lower lumbar vertebrae density of ovariectomized rats was observed and the decrease was prevented by tofu.   Key words : Tofu, tempeh, bone loss,  and ovariectomy
MODEL DINAMIK PENGENDALIAN PENCEMARAN AIR KALI SURABAYA Suwari, Suwari; Riani, Etty; Pramudya, Bambang; Djuwita, Ita
Bumi Lestari Journal of Environment Vol 11 No 2 (2011)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

The Surabaya River plays an important role as water supply of the Surabaya PDAM, irrigation, industry, transportation, and means of recreation. However, domestic, industrial, and agricultural waste that were discharged into the river stream polluted the Surabaya River and decreased the carrying capacity and assimilative capacity. Therefore, effort to monitor and control the Surabaya River water pollution need to be well organized and implemented. The aim of the research is to develop a model of water pollution control on Surabaya River region. The research was carried out based on field survey, in situ and laboratory sample examination, questionnaire, and expert judgment. Pollution control model developed in this study was built into three sub-models, namely: (1) ecology sub-model, (2) social sub-model, and (3) economy sub-model using powersim constructor 2.5 version. Pollution control scenarios were developed using prospective analysis. The results of water pollution parameters such as TSS, DO, BOD, COD, N-NO2, and the level of mercury (Hg) were higher than the allowable class 1 standard. The sources of Surabaya River pollution mainly are domestic and industrial waste with total load of BOD, COD, and TSS are 55.49, 132.58, and 210.13 ton/day, respectively. According to water quality status, the Surabaya River is categorized as heavy polluted and the loading pollution need to be decreased. By using prospective analysis, there were five important factors that affect the future of the Surabaya River water pollution control, i.e.: (1) population growth and community awareness, (2) community perception, (3) implementation of regulations, (4) commitment/local government support, and (5) system and institutional capacity. There are three development scenarios, that are pessimistic, moderate and optimistic. The moderate and optimistic scenario are the realistic scenarios that occur in the future for Surabaya River water pollution control in considering of ecology, social and economy aspects.
Beberapa Aspek Makro dan Mikroanatomi otak tikus (Rattus sp.) yang mengalami hipotiroid Said, Nurhidayat; Djuwita, Ita; Sigit, Koeswinarning
Hemera Zoa Vol 77, No 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Perlakuan hipotiroidosme maternal dan fetal pada tikus sampai berurnur 10 minggu, memberikan dampak pada perkembangan somatis dan otak. Secara kuantitatif, hasil penelitian ini menunjukkan penurunan bobot tubuh dan otak, volume otak. Berdasarkan berat relatif otak terhadap bobot tubuh, pertumbuhan otak tetap menjadi prioritas dalam keadaan hipotiroidisme. Beberapa parameter mikroskopik, menunjukkan penurunan tebal korteks, kepadatan serabut syaraf subkortikal, dia metersel syaraf korteks dan hipokampus juga jumlah sel syaraf dan penunjang pada korteks serebri.
Aktifitas dan pola makan, minum dan memamah biak kancil (Tragulus javanicus) di kebun binatang Ragunan Jakarta dan Surabaya Said, Nurhidayat; Winarto, Adi; Boediono, Arief; Djuwita, Ita; Nisa, Chairun; Wrediati, Tutik; Setijanto, Heru; Fakhrudin, Mohamad
Hemera Zoa Vol 77, No 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Telah dilakukan penelitian pada kancil (Tmgulus javanicus) di Kebun Binatang Ragunan - Jakarta dan Kebun Binatang Wonocolo - Surabaya untuk mendapatkan jenis makanan yang disukai, dan aktifitas makan, minum dan ruminasi dari kancil yang dikandangkan.Pada penelitian ini, disediakan 10 jenis makanan dan empat jenis diantaranya yaitu pisang, kacang panjang, kangkung dan pepaya lebih disukai. Aktifitas makan dilakukan pada siang dan malam hari, dan pada saat istirahat kancil melakukan aktifitas ruminasi. Perilaku makan dan ruminasi ini berbeda dengan perilakunya di habitas asalnya. Sedangkan aktifitas minum, jarang dilakukan, hal ini mungkin disebabkan oleh tingginya kadar air di dalam makanan yang disediakan. Aktifitas makan dari kancil yang dikandangkan telah berubah menjadi diurnal dan nokturnal.
Developmental capacity of goat oocytes collected from 5°C preserved ovaries Djuwita, Ita
Hemera Zoa Vol 2, No 1 (2010): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Research has been conducted to study the effect of ovaries preservation at 5°C on the oocytes development capacity i.e the capacity of oocytes to undergo in vitro maturation (IVF), in vitro fertilization (IVF) and in vitro embryo development. Goat ovaries were abtained from slaughterhouse in saline solution containing 0.1% Bovine Serum Albumine (BSA) and antibiotic and kept at 5°C for 3 and 12 hours. As control, untreated ovaries were kept for 3 hours at 30-35°C, the temperature usually used for ovaries transportation. The oocytes were aspirated from follicles with 2-5 mm in diameter using 20G needle connected to a 5 ml syringe containing modified phosphate buffered saline (mPBS). The aspirated oocytes were incubated in 100 µl  micro drops of tissue culture medium -199 (TCM-199) supplemented with 10% newborn calf serum (NBCS), 0.01 mg/ml follicle stimulating hormore (FSH) and 50  µg/ml   gentamycine sulphate for 24 hours at 38.5 °C in 5% CO2 incubator. In vitro fertilization (IVF) was done in CO2 incubator at 38.5 °C for 18 hours using fresh semen. Inseminated by their nuclear status after aceto-orcein staining. The results showed that the avarage number of morphological normal oocytes collected from 5 °C  preserved  ovaries were significantly lower than from the untreated control ovaries. After 24 h incubation the percentage of matured oocytes from the 3 ang 12 h preserved ovaries were 85.3± 2.8% and 75.5±  2.2%, respectively (P