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Substitution time of natural food by artificial diet on survival rate and growth of pacific white shrimp (Litopenaeus vannamei) postlarvae during rearing in low salinity media Taqwa, Ferdinand Hukama; Djokosetiyanto, Daniel; Affandi, Ridwan
Jurnal Akuakultur Indonesia Vol 10, No 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

This research was conducted to determine natural food substitution time by artificial diet   after salinity acclimatization from 20 ppt until 2 ppt, which can increase survival and growth of (Litopenaeus vannamei) postlarvae during rearing period. Design experiment was completely randomized design with five treatments and three replications of natural food Chironomus sp.  (60% of crude protein) substitution time by artificial diet (40% of crude protein) at day: 1 (A), 7 (B), 14 (C), 21 (D) and full natural food without artificial diet (E) during 28 days rearing period. White shrimp postlarvae and rearing media in this experiment based from best result of earlier research that is PL25 from acclimatization in media 2 ppt with addition of potassium 25 ppm to freshwater media.  The densities of PL25 white shrimp were 20 PLs/50 liters of 2 ppt media. The result of this experiment showed that the use of artificial diet as soon as after salinity acclimatization (PL25) gave best performance production compared to which only that was given natural food Chironomus sp. during experiment or with treatment by artificial diet substitution at day-7, day-14 or day-21, shown with the highest value of food consumption level, protein retention, energy retention, daily growth rate and food efficiency. Survival rate of PL54 was above 80% and not significant different between treatment. That is supported by chemical-physical value of water quality still in range appropriate to survival rate of white shrimp post larvae during a rearing period. The result of this experiment indicated that requirement nutrient of PL25 in low salinity did not fulfilled if only rely on natural food, so that require artificial diet with nutrition content to support growth and survival rate of white shrimp post larvae more maximal. Key words: salinity, natural food, artificial diet, Pacific white shrimp   ABSTRAK Tujuan penelitian ini adalah untuk menentukan waktu penggantian pakan alami oleh pakan buatan yang tepat selama masa pemeliharaan postlarva udang vaname di media bersalinitas rendah setelah melalui masa aklimatisasi penurunan salinitas dari 20 ppt hingga 2 ppt, sehingga dapat meningkatkan pertumbuhan dan kelangsungan hidup. Rancangan percobaan berupa rancangan acak lengkap dengan perlakuan yang diterapkan berupa waktu penggantian pakan alami Chironomus sp. (kadar protein 62%) oleh pakan buatan (kadar protein 40%) pada hari ke-1 (A), ke-7 (B), ke-14 (C), ke-21 (D) dan pakan alami (E) selama masa pemeliharaan. Postlarva udang vaname dan media pemeliharaan yang dipergunakan selama percobaan mengacu pada hasil terbaik yang didapatkan dari penelitian pendahuluan yaitu berupa PL25 hasil aklimatisasi di media bersalinitas 2 ppt  dengan penambahan kalium 25 ppm ke media air tawar pengencer. Padat tebar sebanyak 20 ekor/50 liter/wadah. Hasil percobaan menunjukkan bahwa pemberian pakan buatan yang diberikan segera setelah masa aklimatisasi salinitas (pada awal pemeliharaan PL25) memberikan performa produksi budidaya terbaik bila dibandingkan dengan yang hanya diberi pakan alami selama masa pemeliharaan maupun waktu penggantian pakan alami oleh pakan buatan pada hari ke-7, ke-14 dan hari ke-21 yang ditunjukkan dengan tingkat konsumsi pakan, retensi protein, retensi energi, laju pertumbuhan harian dan efisiensi pakan yang tertinggi.  Kelangsungan hidup di akhir pemeliharaan (PL54)  di atas 80% dan tidak berbeda nyata antar perlakuan. Hal ini ditunjang oleh nilai fisika kimia air yang berada dalam kisaran yang layak selama masa pemeliharaan. Hasil percobaan ini menunjukkan bahwa kebutuhan nutrisi pada stadia PL25 di media bersalinitas rendah tidak terpenuhi jika hanya mengandalkan pakan alami sehingga perlu ditunjang dari pakan buatan dengankandungan nutrisi yang dapat mendukung pertumbuhan dan kelangsungan hidup yang lebih maksimal. Kata kunci: salinitas, pakan alami, pakan buatan, udang vaname.
Enhancement of non-specific immune response, resistance and growth of (Litopenaeus vannamei) by oral administration of nucleotide Manoppo, Henky; Sukenda, .; Djokosetiyanto, Daniel; Sukadi, Mochamad Fatuchri; Harris, Enang
Jurnal Akuakultur Indonesia Vol 10, No 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

This research evaluated the nonspecific immune responsse, resistance, and growth of Litopenaeus vannamei fed nucleotide diet. Shrimp juveniles (mean weight 5.39±0.56 g) were reared in two groups of glass aquaria, each with three replications. Shrimps in group one and group two were fed nucleotide diet and basal diet each for four weeks. Total haemocyte count (THC) and PO activity were evaluated at the end of feeding while growth was measured at two weeks interval. At the end of feeding trial, the shrimps were intramuscularly injected with Vibrio harveyi 0.1x106 cfu.shrimp-1. THC of shrimp fed nucleotide diet significantly increased (P
The Role of Bakau Snail, Telescopium telescopium L., as Biofilter in Waste Water Management of Intensive Shrimp Culture Hamsiah, .; Djokosetiyanto, Daniel; Adiwilaga, E. M.; Nirmala, Kukuh
Jurnal Akuakultur Indonesia Vol 1, No 2 (2002): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

The objective the experiment is to know the role of bakau snail, Telescopium telescopium L., as biofilter for improving waste water quality in shrimp culture.  The experiment was carried out at laboratory scale.  The parameters that observed in this experiment are physical, chemical and biological of waste water.  Growth and survival rate of snail were also observed. Waste water quality measurement was carried out during a week, while the growth and survival rate were measured during two months.  The aquarium of 30x40x40 cm were filled with 30 l of waste water from intensive shrimp culture.  Bakau snail were stocked to the aquarium with density of 0 (control), 6, 9 and 12 snail/aquarium, and these treatment were replicated 3 times.  The result shown that total organic matter (TOM), total ammonia, dissolved oxygen (DO) of waste water, and growth and survival rate of snail were not different between treatment of stocking density, while the biological oxygen demand (BOD), total suspended solid (TSS), nitrite and nitrate were significantly different (p
Water Quality and Sediment Profile in Shrimp Culture with Different Sediment Redox Potential and Stocking Densities Under Laboratory Condition Wiyoto, Wiyoto; Sukenda, Sukenda; Harris, Enang; Nirmala, Kukuh; Djokosetiyanto, Daniel
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 2 (2016): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Sediment quality has been considered as one of the prime factors influencing the environment quality that support maximum shrimp production.The aim of the study was toevaluate the effects of sediment redox potential and shrimp stocking density on the profile of some sediment and water quality parameters. Two factors randomized factorial design was applied, with stocking density (60 and 120 shrimps.m-2) as the first variable and sediment redox potential (-65 mV, -108 mV and -206 mV) as the second variable. Some significant changes in TP, total Mn, and total S concentrations in the sediment were observed after the experimentation (P<0.05). Sediment redox potential significantly affected the dissolved oxygen, TAN, NO2, NO3, and H2S concentrations in the water. Whereas shrimp stocking density affected all water quality parameters except H2S concentration. Significant interactions between redox potential and stocking densities were observed in the nitrite and alkalinity concentrations. The significant effects of both shrimp density and redox potential on the sediment and water parameters in particular those that are known to directly affect the shrimp welfare (e.g. oxygen, ammonia, nitrite and H2S) indicate that these variables are of important aspects in shrimp pond management. Furthermore, the results clearly showed that -206mV redox potential significantly reduced the dissolved oxygen concentration in the sediment-water interface and increased the generation of H2S in water column. Thereby, this redox potential level is not advisable for shrimp culture system. Keywords: redox potential, stocking density.
Water Quality Study for Grouper Mariculture in Divur Bay Dullah Island, Tual City Djokosetiyanto, Daniel; Nawati, Henny Fitri; Machfud, Machfud; Fahrudin, Achmad
Journal Omni-Akuatika Vol 13, No 1 (2017): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

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Water quality is one of the factors that determine the feasibility and success of the development of grouper mariculture. The objective of this research was to analyze water quality which was prepared for grouper mariculture. This research was carried out in Divur Bay, Dullah island of Tual City. The water quality parameters observed were including temperature, brightness, salinity, pH, current velocity, depth, substrate type, DO, nitrate and phosphate in ten stations spread in Divur Bay. Water quality data were analyzed descriptively. The results showed that Divur Bay was feasible for the grouper culture development with a range of values of temperature, brightness, salinity, pH, current velocity, depth, DO, nitrate and phosphate obtained were 30-31oC; 2.28-7.86 m; 33-35 ppt; 7.7-8.1; 0.06-0.617m/s; 2..28-18.58 m; 3.7-4.8 ppm; 0.0015-0.219 ppm; 0.0076-0.0767 ppm.
Survival and growth responses of snakehead fish Channa striata Bloch. juvenile in aerated and unaerated acid sulfate water Purnamawati, ,; Djokosetiyanto, Daniel; Nirmala, Kukuh; Surawidjaja, Enang Harris; Affandi, Ridwan
Jurnal Akuakultur Indonesia Vol 16, No 1 (2017): Jurnal Akuakultur Indonesia
Publisher : ISSA

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ABSTRACT  The aim of the research was to analyze survival rate, specific growth rate, albumin, and feed efficiency and physiological (blood glucose, cortisol, dan haemoglobin) responses of snake head fish juvenil that reared at aerated and unaerated of tidal land water have been conducted in the laboratory. Experiments using completely randomized design with aerated and unaerated as a treatment, and each treatment has twelve replications. The snakehead fish juvenil wich length 2.4±0.2 cm and weight of 0.21±0.05 g reared in the aquarium that are size 30×25×35 cm (water volume 25 L) with a stocking density 2 juvenile/L, for 40 days. The fishes were fed with commercial feed with protein content about 40%, feeding two times a day (morning and afternoon) were at satiation. Replacement of water done every two days about 10% of the total water volume in the aquarium. The results showed that unaerated median significantly affected to biometric and physiological response of juvenile of snake head fish. The media un-aerated gives the best results shown by the higher value of survival (92%), specific growth rate (6.73%/ day), feed efficiency (78.22%), protein retention (41.91%), energy retention (30.81%) value of albumin (6.60 g/100 mL) and the haemoglobin (5.58 g/dL), and have the lowest value of cortisol (21.49 ng/L) and blood glucose (43.36 mg/100 mL). Keywords: acid sulfate water, growth rate, aeration, Channa striata   ABSTRAK  Penelitian ini bertujuan untuk menganalisis respons kelangsungan hidup, pertumbuhan spesifik, albumin, dan efisiensi pakan dan fisiologis (kortisol, glukosa darah, dan hemoglobin) juvenil ikan gabus yang dipelihara dengan dan tanpa aerasi pada media air rawa pasang surut. Penelitian menggunakan rancangan acak lengkap dengan aerasi dan tanpa aerasi sebagai perlakuan, dan masing-masing perlakuan memiliki 12 ulangan. Juvenil ikan gabus berukuran panjang 2,4±0,3 cm dan bobot 0,21±0,03 g dipelihara dalam akuarium 30×25×35 cm (volume 25 L) dengan padat tebar 2 ekor/L, selama 40 hari. Ikan diberi pakan berupa pakan komersial dengan kadar protein ±40%, pemberian pakan dua kali sehari (pagi dan sore) at satiation. Penggantian air dan penyiponan dilakukan dua hari sekali sebanyak 10% dari volume total dalam akuarium. Hasil penelitian menunjukkan bahwa perlakuan tanpa aerasi berpengaruh nyata terhadap respons biometrik dan fisiologis juvenil ikan gabus. Media tanpa aerasi memberikan hasil yang lebih baik ditunjukkan oleh kelangsungan hidup (92%), laju pertumbuhan spesifik (6,73%/ hari), efisiensi pakan (78,22%), retensi protein (41,91%), retensi energi (30,81%), kadar albumin (6,60 g/100 mL), dan hemoglobin (5,85 g/dL) yang lebih tinggi, sedangkan kadar kortisol (219 ng/L) dan glukosa darah (43,36 mg/100 mL) yang terendah. Kata kunci: media sulfat masam, pertumbuhan, aerasi, Channa striata
The concentration of optimum dissolved oxygen levels for growth of mangrove crab Scylla serrata seed in recirculation system Faturrohman, Kurnia; Nirmala, Kukuh; Djokosetiyanto, Daniel; Hastuti, Yuni Puji
Jurnal Akuakultur Indonesia Vol 16, No 1 (2017): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT This study aimed to determine optimum dissolved oxygen (DO) through the addition of aeration and to evaluate the role of dissolved oxygen on production performance and stress responses of mangrove crab Scylla serrata. Experimental design used was complete randomized design with four treatments namely no aeration (A), one point aeration (B), two points aeration (C), and three points aeration (D). All treatments replicated three times. The crab with the average of body weight 45.6±2.1 g/individual cultured in a plastic box (40×30×30 cm3). The stocking densities was 10 crab/box. Crab was cultured within 42 days and were fed two times a day by restricted method (15% of the total biomass). The result showed that C treatment produced 5.51 mg/L dissolved oxygen and gave the best result of mangrove crabs production performance  with 60% survival, 0.83±0.03 g/day absolute growth rate and food conversion ratio 1.1. It also showed good response to the stress that indicated by the cortisol level (10.159 µg/dL). The best results of coefficient of diversity showed by D treatment that was 13.5%. The water quality during study period was fluctuative as affected by different dissolved oxygen value. Keyword: mangrove crabs, dissolved oxygen, production performance  ABSTRAK Penelitian ini bertujuan menentukan kadar oksigen terlarut (OT) atau dissolved oxygen (DO) yang optimum melalui penentuan titik aerasi serta mengevalusi peranan oksigen terlarut terhadap kinerja produksi dan respons stres kepiting bakau Scylla serrata. Rancangan penelitian yang digunakan adalah rancangan acak lengkap dengan empat pelakuan (penambahan titik aerasi dengan rincian A, tidak menggunakan titik aerasi; B, satu titik aerasi; C, dua titik aerasi dan D, tiga titik aerasi) dan tiga ulangan. Kepiting bakau yang digunakan memiliki berat rata-rata 45,6±2,1 g/ekor dengan padat tebar 10 ekor/wadah. Wadah yang digunakan selama pemeliharaan adalah bak fiber plastik yang berukuran 40×30×30 cm3. Pemeliharaan kepiting bakau dilaksanakan selama 42 hari dan diberikan pakan dua kali sehari dengan metode restricted yakni sebesar 15% dari biomassa kepiting. Hasil penelitian menunjukkan bahwa perlakuan C yaitu penambahan dua titik aerasi menghasilkan nilai kelarutan oksigen rata-rata sebesar 5,51 mg/L dan memberikan hasil terbaik terhadap kinerja produksi kepiting bakau (tingkat kelangsungan hidup 60%; laju pertumbuhan mutlak 0,83±0,03 g/hari; dan rasio konversi pakan 1,1). Perlakuan C juga menunjukkan respons stres yang baik dengan memiliki nilai kortisol paling rendah dari perlakuan lain yaitu 10,159 µg/dL. Untuk parameter koefisien keragaman berat, hasil terbaik terjadi pada perlakuan D sebesar 23,3%. Kualitas air selama penelitian memiliki nilai yang fluktuatif di setiap perlakuan sebagai efek adanya perbedaan nilai kelarutan oksigen yang dihasilkan. Kata kunci: kepiting bakau, kelarutan oksigen, kinerja produksi
NONSPECIFIC IMMUNE RESPONSE AND RESISTANCE OF Litopenaeus vannamei FED WITH NUCLEOTIDE, β-GLUCAN, AND PROTAGEN DIETS Manoppo, Henky; Sukenda, Sukenda; Djokosetiyanto, Daniel; Sukadi, Mochamad Fatuchri; Harris, Enang
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

The objective of this research was to evaluate the nonspecific immune response and resistance of Litopenaeus vannamei fed with nucleotide, β–glucan, and protagen diets. Shrimp juveniles with an average weight of 5.39±0.56 g were reared in glass aquaria at a density of 15 shrimps/aquarium. Shrimps were fed three times a day for four weeks at a feeding rate of 3%/bw/day. Treatment diets consisted of A: basal diet (without immunostimulant), B: β–glucan, C: protagen, and D: nucleotide, each with three replicates. At the end of feeding period, the shrimps were intramuscularly injected with Vibrio harveyi 0.1 x 106 cfu.shrimp-1. Total haemocyte count (THC) of shrimp fed with nucleotide-diet was significantly different compared to that of control shrimp (p=0.01), but not different compared to shrimp fed with protagen-diet. PO activity also increased significantly in shrimp fed with nucleotide-diet (p=0.02). β–glucan diet could also increase THC and PO activity, but compared to the control, the increase was not significantly different. Overall, PO activity of shrimp fed with nucleotide, β–glucan, and protagen diets was high (&gt;0.35). Oral administration of nucleotide, β–glucan, and protagen for four consecutive weeks significantly increased resistance of shrimp to disease (&lt;0.01) where the highest resistance rate was observed on shrimp fed with nucleotide-diet. Growth of shrimp fed with nucleotide-diet was significantly different compared to that of control shrimp (p&lt;0.01), as well as to β–glucan, and protagen-treated shrimp. As a conclusion, supplementation of nucleotide into shrimp pellet enhanced nonspecific immune response and growth performance better than β-glucan, and protagen.
PENGARUH PENAMBAHAN KALIUM PADA MASA ADAPTASI PENURUNAN SALINITAS TERHADAP PERFORMA PASCALARVA UDANG VANAMEI (Litopenaeus vannamei) Taqwa, Ferdinand Hukama; Djokosetiyanto, Daniel; Affandi, Ridwan
Jurnal Riset Akuakultur Vol 3, No 3 (2008): (Desember 2008)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

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Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan kalium selama masa adaptasi penurunan salinitas terhadap performa pascalarva udang vanamei. Penelitian ini dilaksanakan untuk mendapatkan kadar kalium optimal yang dapat menurunkan tingkat stres dan meningkatkan sintasan pascalarva udang vanamei setelah melalui masa adaptasi penurunan salinitas. Hewan uji yang digunakan adalah PL20 udang vanamei. Rancangan percobaan menggunakan rancangan acak lengkap dengan perlakuan yang diterapkan adalah penambahan kalium ke air tawar pengencer masing-masing sebanyak 0 mg/L (A), 25 mg/L (B), 50 mg/L (C), dan 75 mg/L (D). Penurunan salinitas dilakukan secara gradual selama 4 hari dari salinitas 25 ppt hingga mencapai 2 ppt. Hasil percobaan menunjukkan bahwa penambahan kalium sebanyak 25 mg/L hingga kadar kalium media menjadi 51 mg/L dapat mengurangi pembelanjaan energi untuk osmoregulasi, tingkat stres, dan laju metabolisme standar sehingga meningkatkan sintasan pascalarva udang vanamei setelah melalui masa adaptasi penurunan salinitas selama 96 jam (4 hari).The objective of this research was to study the effect of potassium addition during salinity acclimatization from 25 ppt down to 2 ppt on the performance of Litopenaeus vannamei postlarvae. This experiment was done to determine optimal dosage of potassium which can reduce stress level and increase survival rate of L. vannamei postlarvae after salinity acclimatization. Specimen test used was PL20 of white shrimp (0.001 g). Experimental design used completely randomized design with four treatments and three replications of different potassium addition levels to freshwater: 0 mg/L (A), 25 mg/L (B), 50 mg/L (C), and 75 mg/L (D). Dilution of salinity was done gradually using freshwater during 4 days from 25 ppt down to 2 ppt. The result of this experiment indicated that the addition of 25 mg/L potassium (potassium level in media was 51 mg/L) reduced the energy cost for osmoregulation, level of stress and standard metabolism rate of PL, resulting in the increase of survival rate after completing a period of salinity acclimatization during 4 days.
ANALISIS FAKTOR PENTING DALAM PENGELOLAAN PERIKANAN BUDIDAYA DI KERAMBA JARING APUNG BERKELANJUTAN DENGAN METODE Interpretative Structural Modeling (ISM) DI WADUK CIRATA, JAWA BARAT Widiyati, Ani; Djokosetiyanto, Daniel; Bengen, Dietriech; Kholil, M.; Arifin, Zainal
Jurnal Riset Akuakultur Vol 4, No 2 (2009): (Agustus 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

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Permasalahan pencemaran akibat keberadaan keramba jaring apung yang terjadi di  perairan Waduk Cirata sangat kompleks. Untuk mengatasi permasalahan tersebut  baik secara teknis maupun non teknis diperlukan pendekatan kesisteman. Interpretation Structural Modelling (ISM) merupakan salah satu metode kesisteman yang dapat digunakan untuk menganalisis faktor non teknis (kelembagaan) yang penting dalam pengelolaan perikanan budidaya di keramba jaring apung secara berkelanjutan di Waduk Cirata. Penelitian ini bertujuan untuk menganalisis faktor penting yang berpengaruh dalam pengelolaan perikanan budidaya di keramba jaring apung secara berkelanjutan. Hasil penelitian terdapat 3 faktor penting yang mempengaruhi keberhasilan dalam pengelolaan perikanan budidaya di keramba jaring apung secara berkelanjutan. Tiga faktor penting tersebut adalah faktor tujuan program, kebutuhan progam dan lembaga yang berperan.The problems of pollution as the negative effect of the existence of floating cage culture in Cirata Reservoir are very complex. To deal with those problems using both technical and non technical ways, a system approach is needed. Interpretation Structural Modelling (ISM) is one of system methods that can be used to analyse non technical factors such as the influence of institutions that is important in the management of sustainable floating cage culture at Cirata Reservoir. The study was aimed to analyse the important factors that influence the management of sustainable floating cage culture. The result of the study showed that there were three important factors that can determine the success in managing sustainable floating cage fisheries. Those three factors were the program purpose, needed program and role of institution.