Soemitro Djojowidagdo
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Karakterisasi protease Bacillus sp. UGM5 Widowati, Titik Purwati; Djojowidagdo, Soemitro; Setiyono, Setiyono; Asmara, Widya
Majalah Kulit, Karet, dan Plastik Vol 14, No 26 (1999): Majalah Barang Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1359.555 KB) | DOI: 10.20543/mkkp.v14i26.297

Abstract

The objective of this experiment is to indentify the characters of proetease produced by Bacillus sp.UGM5.the protease secreted by Bacillus sp.UGM5 was first isolated,purified and then charactirezed.The crude enzyme has spesific actifity of 1.14 U/mg,however,the spesific activity of purified enzyme was increased by 23.8 times fold and recovery was 33.69%.The Page of nondenatured crude enzymes showes two type of proreases,however ,the SDS-Page of denatured purified enzyme showed four protein-bends with molecular weights of 55.5 kDa,18kDa respecetively.The optimum pH and temperature for the enzyme acrivity are 8.5 and 420C and belongs to serin protease type,with Km 3 X 10-3mM and Vmax 0.0890mM/30 minutes.The activity is not inhibited by Ca+2,Fe+2 and EDTA. INTISARITujuan penelitian ini adalah untuk mengkarakterisasi  protease yang diproduksi  Bacillus sp. UGM5. Protease yang disekresi oleh Bacillus sp.UGM5 pertama-tama diisolasi,dipurifikasi kemudian dikarakterisasi.Enzim kasar mempunyai spesifik 1,14 U/mg.Aktivitas spesifik enzim yang dimurnikan meningkat 23,8 kali dengan recovery 33,69%.Elektroforesis gel poliakrilamid enzim yang dipurifikasi dan didenaturisasi menunjukan ada empat pita protein dengan berat protein masing-masing 55,5 kDa,51 kDa,18 kDa,dan 15,5 kDa .ph dan temperatur optimum aktivitas enzim adalah 8,5 dan 420C,termasuk tipe protease serin,dengan nilai Km 3 X10-3mM dan Vmax 0,0890mM/30menit.Aktivitas enzim tidak dihambat oleh adanya ion Ca2+,Fe+3 dan EDTA.
Karakterisasi protease Bacillus sp. UGM5 Widowati, Titik Purwati; Djojowidagdo, Soemitro; Setiyono, Setiyono; Asmara, Widya
Majalah Kulit, Karet, dan Plastik Vol 14, No 26 (1999): Majalah Barang Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1359.555 KB) | DOI: 10.20543/mkkp.v14i26.297

Abstract

The objective of this experiment is to indentify the characters of proetease produced by Bacillus sp.UGM5.the protease secreted by Bacillus sp.UGM5 was first isolated,purified and then charactirezed.The crude enzyme has spesific actifity of 1.14 U/mg,however,the spesific activity of purified enzyme was increased by 23.8 times fold and recovery was 33.69%.The Page of nondenatured crude enzymes showes two type of proreases,however ,the SDS-Page of denatured purified enzyme showed four protein-bends with molecular weights of 55.5 kDa,18kDa respecetively.The optimum pH and temperature for the enzyme acrivity are 8.5 and 420C and belongs to serin protease type,with Km 3 X 10-3mM and Vmax 0.0890mM/30 minutes.The activity is not inhibited by Ca+2,Fe+2 and EDTA. INTISARITujuan penelitian ini adalah untuk mengkarakterisasi  protease yang diproduksi  Bacillus sp. UGM5. Protease yang disekresi oleh Bacillus sp.UGM5 pertama-tama diisolasi,dipurifikasi kemudian dikarakterisasi.Enzim kasar mempunyai spesifik 1,14 U/mg.Aktivitas spesifik enzim yang dimurnikan meningkat 23,8 kali dengan recovery 33,69%.Elektroforesis gel poliakrilamid enzim yang dipurifikasi dan didenaturisasi menunjukan ada empat pita protein dengan berat protein masing-masing 55,5 kDa,51 kDa,18 kDa,dan 15,5 kDa .ph dan temperatur optimum aktivitas enzim adalah 8,5 dan 420C,termasuk tipe protease serin,dengan nilai Km 3 X10-3mM dan Vmax 0,0890mM/30menit.Aktivitas enzim tidak dihambat oleh adanya ion Ca2+,Fe+3 dan EDTA.