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PENGARUH CARA PENAMBAHAN BAKTERI PROBIOTIK DALAM SUSU KEDELAI TERHADAP KADAR KOLESTEROL DARAH TIKUS HIPERKOLESTEROLEMIA Djide, M. Natsir; Wahyudin, Elly; Sartini, -
MAJALAH FARMASI DAN FARMAKOLOGI Vol 15, No 1 (2011)
Publisher : Universitas Hasanuddin

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Abstract

Peningkatan kadar kolesterol pada manusia merupakan salah satu resiko yang berhubungan dengan penyakit-penyakit kardiovaskuler. Kandungan bioaktif kedelai diketahui memiliki kemampuan menurunkan kadar kolesterol darah. Peranan bakteri probiotik dalam susu kedelai mempunyai efek sinergi. Cara penambahan bakteri probiotik dalam susu kedelai dapat mempengaruhi efek penurunan kolesterolnya. Tujuan penelitian ini untuk mengetahui pengaruh cara penambahan bakteri probiotik ke dalam susu kedelai terhadap efek penurunan kolesterol pada tikus albino (Rattus novergicus) strain Wistar yang dibuat hiperkolesterolemik. Dua cara penambahan bakteri probiotik dilakukan ke dalam susu kedelai. Yang pertama, bakteri probiotik ditambahkan ke dalam susu kedelai selanjutnya difermentasi selama 24 jam. Kedua, bakteri probiotik ditambahkan ke dalam susu kedelai tanpa difermentasi. Uji efek penurunan kolesterol dilakukan pada tikus putih galur Wistar. Hasil penelitian menunjukkan bahwa penambahan bakteri probiotik ke dalam susu kedelai tanpa fermentasi memberikan efek penurunan kolesterol yang lebih baik dengan persentase penurunan total kolesterol sebesar 41,85 % dan ratio LDL/HDL sebesar 0,22.
PENGARUH PEMAKAIAN SETENGAH VOLUME PADA PEMERIKSAAN UREA TERHADAP NILAI SIMPANGAN BAKU DAN KOEFISIEN VARIASI DENGAN MENGGUNAKAN SERUM KONTROL Hudaya, Nurul; Subehan, .; Lidjaja, Agnes; Djide, M. Natsir
MAJALAH FARMASI DAN FARMAKOLOGI Vol 17, No 3 (2013)
Publisher : Universitas Hasanuddin

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Telah dilakukan penelitian tentang pengaruh pemakaian setengah volume pada pemeriksaan urea terhadap nilai SD (standard deviation) dan CV (coefisien variation) dengan menggunakan serum kontrol. Tujuan dari penelitian ini adalah untuk mengetahui validitas dari pemakaian setengah volume pada pemeriksaan urea. Penelitian ini dilakukan dengan jumlah sampel sebanyak 40 (20 sampel volume penuh dan 20 sampel setengah volume). Hasil penelitian menunjukkan data pengerjaan volume penuh diperoleh nilai rata-rata ( ) yaitu 95,9; nilai SD yaitu 6,77; nilai CV yaitu 7,06%. Sedangkan hasil pengerjaan setengah volume diperoleh nilai rata-rata ( ) yaitu 112,3; nilai SD yaitu 18,9; niai CV yaitu   16,82 %. Berdasarkan hasil penelitian yang telah dilakukan diperoleh kesimpulan bahwa pemakaian setengah volume tidak dianjurkan penggunaannya dalam laboratorium karena diperoleh nilai penyimpangan dari hasil pemeriksaan terhadap nilai rata-rata yang dinyatakan dengan CV sebesar 16,82 %, dimana nilai hasil tersebut jauh dari nilai standar batas maksimum CV sebesar  8%  yang ditetapkan oleh Departemen Kesehatan RI.
Ekstraksi Isoflavon Kedelai dan Penentuan Kadarnya Secara Ultra Fast Liquid Chromatography (UFLC) Sartini, .; Djide, M. Natsir; Permana, A. Dian; Ismail, .
SAINSMAT Vol 3, No 2 (2014): September
Publisher : SAINSMAT

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Abstract

Isoflavon merupakan komponen polifenol utama dalam kacang kedelai. Isoflavon kedelai utamanya dalam bentuk glikosida (Genistin dan Daidzin) dibanding bentuk aglikonnya (Genistein dan Daidzein). Tujuan penelitian ini adalah untuk mengetahui pengaruh metode ekstraksi terhadap kandungan isoflavonnya (Genistein, Daidzein, Genistin, Daidzin). Isoflavon kedelai diekstraksi dengan beberapa cara, yaitu: 1) kacang kedelai tanpa kulit ari diblender dengan air panas, 2). Kacang kedelai tanpa kulit ari diblender dengan etanol 70 % , 3) kacang kedelai utuh diekstraksi secara maserasi dengan aseton 70 %, dan 4) kacang kedelai utuh diekstraksi secara maserasi dengan metanol.. Perbandingan kacang kedelai dan cairan penyari 1:10. Ekstrak yang diperoleh dianalisis kadar isoflavonnya menggunakan kromatografi cair kecepatan tinggi. Hasil penelitian menunjukkan total isoflavon tertinggi (dihitung sebagai total genistein, daidzein, genistin, daidzin) ada pada kacang kedelai utuh yang diekstraksi dengan metanol.Kata Kunci : Metode Ekstraksi, Isoflavon, Kedelai (Glycine max L.), Ultra Fast Liquid Chromatography
PRODUKSI SENYAWA BAKTERIOSIN SECARA FERMENTASI MENGGUNAKAN ISOLAT BAL Enterococcus faecium DU55 DARI DANGKE Razak, Abd. Rahman; patong, Abd. Rauf; Harlim, Tjodi; Djide, M. Natsir; Haslia, Haslia; Mahdalia, Mahdalia
Indonesia Chimica Acta Volume 2 No 2 - December 2009
Publisher : Indonesia Chimica Acta

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Dangke is one of traditional food from Enrekang Province Sulawesi Selatan which is made from buffalo milk and enzimatically processed using papain from papaya’s gland secretion. Research on this local product as source of lactic acid bacteria (LAB) has been done. Counted 30 LAB was successfully isolated and 3 of them were potentially to be able to yield antimicrobial compound. Enterococcus faecium DU55 is one of LAB isolate available to be applied for producing bacteriocin compound through fermentation. Optimum condition of fermentation was specified by determining the highest antimicrobial activity generated by filtrate of fermentation result. Antimicrobial activity examination was carried out through diffusion agar method by measuring inhibition zone growth of pathogen bacterium Salmonella typhimurium FNCC 0050. Research results indicate that maximum bacteriocin compound production by BAL isolate E. faecium DU55 was obtained at condition of optimum fermentation at 30 °C during 42 hour using M1 medium with the same composition to medium MRS (Man Rogosa and Sharpe).Keyword: dangke, LAB isolate, bacteriocin, antimicrobial activity
PRODUKSI SENYAWA BAKTERIOSIN SECARA FERMENTASI MENGGUNAKAN ISOLAT BAL Enterococcus faecium DU55 DARI DANGKE Razak, Abd. Rahman; patong, Abd. Rauf; Harlim, Tjodi; Djide, M. Natsir; Haslia, Haslia; Mahdalia, Mahdalia
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 2 No 2 - December 2009
Publisher : Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v2i2.977

Abstract

Dangke is one of traditional food from Enrekang Province Sulawesi Selatan which is made from buffalo milk and enzimatically processed using papain from papaya’s gland secretion. Research on this local product as source of lactic acid bacteria (LAB) has been done. Counted 30 LAB was successfully isolated and 3 of them were potentially to be able to yield antimicrobial compound. Enterococcus faecium DU55 is one of LAB isolate available to be applied for producing bacteriocin compound through fermentation. Optimum condition of fermentation was specified by determining the highest antimicrobial activity generated by filtrate of fermentation result. Antimicrobial activity examination was carried out through diffusion agar method by measuring inhibition zone growth of pathogen bacterium Salmonella typhimurium FNCC 0050. Research results indicate that maximum bacteriocin compound production by BAL isolate E. faecium DU55 was obtained at condition of optimum fermentation at 30 °C during 42 hour using M1 medium with the same composition to medium MRS (Man Rogosa and Sharpe).Keyword: dangke, LAB isolate, bacteriocin, antimicrobial activity
Isolasi Dan Identifikasi Bakteri Termofil Penghasil Amilase Dari Sumber Air Panas Lejja Sulawesi Selatan Arfah, Rugaiyah A.; Patong, Abd. Rauf; Ahmad, Ahyar; Djide, M. Natsir
Al-Kimia Vol 2, No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v2i2.1644

Abstract

A Research isolation and identification of bacteria termofil amylase from hot springs Lejja South Sulawesi has been done. This study aims to characterize the morphological, biochemical, genus and species of bacteria producing  the enzyme amylase. The method used in this study through the stages: 1) Skrening and isolation of bacteria by means of as much as 1.0 mL of sample dilution plated on Petri dishes containing agar medium, then incubated for  20-24 hours at 50 °C, colonies of bacteria growing and has a colony morphology different character each taken 1 ose then etched into the amylolytic selective medium then incubated for 20-24 hours at 40oC and 50oC. Colonies that grew on selective media is scratched quadrant amylolytic to obtain pure isolates. Pure bacterial isolates taken 1 ose then grown in selective medium for 48 h at 50° C, bacterial isolates were grown spilled iodine solution (2% I2 and 0.2% KI) when there is a clearing zone around the colony indicated as the enzyme-producing bacterial isolates termofil amylase; 2) termofil characterization of bacterial isolates in microscopy with Gram stain; 3) isolates selected biochemical tests performed according  to the method Bergeys Manual and Systematic of Bacteriology. Results of screening and isolation of 10 bacterial isolates obtained amylase through iodine test, selected 2 isolates, 1 isolate from water samples RSAII-1B and 1 isolates from water samples mixed sediment RSSII-4B, which has a diameter of clearing zone of 5.6 cm respectively and 5.15 cm; out such characterization results of gram stain microscopy showed that the 2 isolates including gram + and shaped bacillus, the colony morphology as observed macroscopically, microscopy and  biochemical test results  obtained  RSAII isolates and isolates RSSII-1B-4B is a Bacillus sp.
Isolasi dan Implementasi Protein Bioaktif Kepah (Atactodea striata) Sebagai Bahan Obat Antibakteri Hasan, Tahirah; Patong, Abd. Rauf; Wahab, Abd. Wahid; Djide, M. Natsir
Al-Kimia Vol 2, No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v2i2.1652

Abstract

This study aimed to 1) determine the degree of saturation of ammonium sulfate right to extract and purify the bioactive protein from shells (Atactodea striata), 2) determine the fraction of active protein from shells (Atactodea striata) as a potential antibacterial. In this study used the Lowry method for determine protein concentration and agar diffusion method for antibacterial activity. Extraction of shells Atactodea striata was conducted by making use of buffer solution (0,1 M Tris-HCl of pH 8.3, 2 M NaCl, 0.01 M CaCl2, 1 % β-mercaptoethanol, and  0.5 % Triton X-100). Purification of proteins by  precipitation using ammonium sulfate at saturation level 30 %, 50 %, 70 %, and 90 %. The results showed that the protein concentration of the crude   extract is 41.6354 mg/mL. At fractionation rate of 0-90% saturation showed the highest concentration of protein found in fractions with 70% saturation level is 56.4184 mg/mL. The testing of antibacterial activity against Staphylococcus aureus showed that crude extracts and protein fractions Atactodea striata is considered effective as an antibacterial. The highest bioactivity during 24-hour incubation in protein fractions obtained by ammonium sulfate saturation level of 50% is 25.17 mm. Whereas the lowest activity was obtained at 90% saturation level is 14.05 mm. Bioactivity against Escherichia coli after incubation for 24 hours has the highest activity in the protein fraction with 30% ammonium sulfate saturation is 15.12 mm. Whereas the lowest activity was showed at 70% saturation level is 10.30 mm. After the observation was continued for 48 hours on both test bacteria, which formed a clear area becomes cloudy. It shows that the crude extract and fractions of protein tend to be bacteriostatic against Staphylococcus aureus and Escherichia coli.