Kis Djamiatun
Magister Ilmu Biomedik Fakultas Kedokteran Universitas Diponegoro

Published : 9 Documents
Articles

Found 9 Documents
Search

Pengaruh Ekstrak Etanol Daun Kelor (Moringa oleifera) pada Ekspresi Insulin dan Insulitis Tikus Diabetes Melitus Sulistyorini, Ratna; Sarjadi, -; Johan, Andrew; Djamiatun, Kis
Majalah Kedokteran Bandung Vol 47, No 2 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar | Full PDF (461.161 KB)

Abstract

Kandungan quercetin pada Moringa oleifera secara ilmiah memiliki potensi sebagai antioksidan dan anti-inflamasi dengan menghambat aktivitas NF-ĸB, serta triterpenoid menstimulasi regenerasi sel β pankreas dan meningkatkan sekresi insulin. Penelitian ini bertujuan melihat pengaruh pemberian ekstrak etanol daun Moringa oleifera terhadap insulitis dan ekspresi insulin pankreas tikus Sprague-Dawley jantan. Penelitian dilakukan di laboratorium Unit Pangan dan Gizi, Pusat Antar Universitas Universitas Gadjah Mada serta Laboratorium Histologi dan Biologi Sel Fakultas Kedokteran Universitas Gadjah Mada, periode Mei–Juli 2013. Penelitian ini menggunakan desain randomized post test only controlled group. Hewan coba sebanyak 24 ekor tikus, diperiksa kadar gula darahnya pada hari ketiga setelah induksi streptozotosin, untuk melihat tikus sudah dalam keadaan hiperglikemik. Tikus kemudian  dibagi dalam tiga kelompok: satu kelompok kontrol dan  dua kelompok perlakuan yang  diberi ekstrak etanol daun Moringa oleifera dosis 250 dan 500 mg/kg/hari selama 21 hari. Gambaran histopatologik dinilai  derajat insulitis pada pulau Langerhans dan dinilai ekspresi insulin dengan skor Allred. Uji statistik yang digunakan adalah Kruskal Wallis dilanjutkan Mann-Whitney dengan taraf kemaknaan p<0,05 karena data tidak berdistribusi normal. Derajat insulitis dan ekspresi insulin pankreas tikus pada kedua kelompok perlakuan menunjukkan perbedaan bermakna dibanding dengan kelompok kontrol (p<0,005). Pada penelitian ini dapat disimpulkan bahwa ekstrak etanol daun Moringa oleifera dosis 250 dan 500 mg/kg menyebabkan ekspresi insulin lebih tinggi dan derajat insulitis lebih rendah dibanding dengan kelompok kontrol.[MKB. 2015;47(2):69–76]Kata kunci: Ekspresi insulin, insulitis, Moringa oleifera, streptozotosinEffect of Ethanol Extract of Moringa oleifera Leaves on Insulin Expression and Insulitis in Diabetes Mellitus RatsAbstractQuercetin content in Moringa oleifera has scientific potential antioxidant and anti-inflammation effects by inhibiting the activity of NF-ĸB. In addition, triterpenoids stimulate pancreatic β cell regeneration and increases insulin secretion. This study aimed to discover the effect of  Moringa oleifera extract on insulitis and insulin expression in pancreas of  Sprague-Dawley male rats. The study was performed at the laboratory of Food and Nutrition Unit of The Inter University Center of Gadjah Mada University and the laboratory of Histology and Cell Biology Faculty of Medicine  Gadjah Mada University during May–July 2013. This was a randomized post test only controlled group study. Twenty four rats were included. Blood glucose test was performed on the third day after streptozotocin induction to ensure that all rats were in hyperglycemic condition. The rats were then divided into three groups: one control group and two treatment groups receiving Moringa oleifera ethanolic leaves extract for 21 days in doses of 250 and 500 mg/kg/day, respectively. Histopathological analysis of pancreas were performed by evaluating insulitis  based on the infiltration of mononuclear inflammatory cells to the Langerhans islets. Insulin expression was evaluated using Allred score. Statistical analysis used was Kruskal-Wallis test, followed by  Mann-Whitney  with a level of significance of p<0.05. The insulitis degree and insulin expression of the two treatment groups were significantly  different compared to the control group (p<0.005). It can be concluded that Moringa oleifera ethanolic leaf extract doses of 250 and 500 mg/kg/day cause higher insulin expression and lower  insulitis degree than in the control group. [MKB. 2015;47(2):69–76]Key words: Insulin expression, insulitis, Moringa oleifera, streptozotocin DOI: 10.15395/mkb.v47n2.456
Efek Moringa oleifera terhadap Gula Darah dan Kolagen Matrik Ekstraseluler Sel β Pankreas Diabetes Eksperimental Ambarwati, Ambarwati; Sarjadi, Sarjadi; Johan, Andrew; Djamiatun, Kis
Jurnal Kedokteran Brawijaya Vol 28, No 2 (2014)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Original Source | Check in Google Scholar | Full PDF (14.772 KB)

Abstract

Daun Moringa oleifera mengandung zat-zat bioaktif yang memperbaiki dan merangsang sel β pankreas untuk mensekresi insulin yang mengatur kadar gula darah. Kelangsungan hidup dan fungsi sel β pankreas memerlukan matriks ekstraseluler (ECM) yaitu fibronektin, laminin dan kolagen. Apakah kadar gula dan kolagen ECM yang mungkin terpengaruh streptozotocin yang merusak sel β pankreas diteliti pada diabetes eksperimental yang diberi perlakukan  M.oleifera. Studi ini menggunakan 24 tikus. Kadar gula darah diukur sebelum dan sesudah induksi streptozotocin 2 hari yang berdampak hiperglikemi, sesudah itu tikus dibagi menjadi 4 kelompok yang terdiri dua grup kontrol yang diterminasi pada hari 0 dan 21 hiperglikemi, dan dua kelompok diperlakukan dengan ekstrak daun M.oleifera dosis 250 dan 500mg/kg BB/hari selama 21 hari. Data dianalisis dengan statistik parametrik dan non-parametrik. Taraf kemaknaan p<0,05. Kadar gula menurun pada masing-masing kelompok perlakukan dosis 250 dan 500mg/BB/kg BB/hari (p=0,000). Kadar gula menjadi normal pada kelompok perlakuan dosis 500mg/kg BB/hari. Ketebalan kolagen ECM pulau Langerhans pankreas tetap normal di semua kelompok penelitian. Ekstrak daun M.oleifera dosis 500 mg/kg BB/hari adalah dosis efektif untuk menormalkan  kadar gula darah pada diabetes eksperimental.Kata Kunci: Diabetes melitus, kadar gula darah, ketebalan kolagen ECM, Moringa oleifera, Streptozotocin,  sel β pankreas
Pengaruh yogurt sinbiotik pisang terhadap kadar glukosa dan insulin tikus sindrom metabolik Rahmawati, Fiqhi Cahyaningrum; Djamiatun, Kis; Suci, Nyoman
Jurnal Gizi Klinik Indonesia Vol 14, No 1 (2017): Juli
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Original Source | Check in Google Scholar

Abstract

Background: The metabolic syndrome often begins with insulin resistance characterized by hyperinsulinemia and hyperglycemia. Synbiotic yoghurt tanduk banana contains probiotic and prebiotic FOS can improve insulin resistance in metabolic syndrome.Objective: To prove the effect of synbiotic yoghurt tanduk banana can reduce blood glucose levels and insulin levels in metabolic syndrome rats.Method: True-experimental study with randomized controlled group pre-post test design in male wistar rats. Eighteen samples were induced to become metabolic syndrome with high fat fructose diet for 2 weeks and they were divided into 3 groups: control (K) was given standard diet, treatment group 1 (P1), and treatment group 2 (P2) were given sinbiotic yoghurt tanduk banana dose of 0.009 ml/g body weight (BW)/day and 0.018 ml/g BW/day for 2 weeks.Results: The paired t-test showed there were differences of blood glucose levels and insulin levels before and after treatment in P2 group. The Kruskal Wallis test showed there were differences of mean blood glucose levels inter group after treatment (p<0.05). The One-way Anova test showed there were differences of mean insulin levels inter group after treatment (p<0.05). Conclusion: Synbiotic yoghurt tanduk banana can reduce blood glucose levels and insulin levels in metabolic syndrome rat with effective dose is 0.018 ml/grBW/day.
Pengaruh pemberian yogurt sinbiotik tepung pisang tanduk terhadap profil lipid tikus sindrom metabolik Octavia, Zana Fitriana; Djamiatun, Kis; Suci, Nyoman
Jurnal Gizi Klinik Indonesia Vol 13, No 4 (2017): April
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Original Source | Check in Google Scholar

Abstract

Background: The metabolic syndrome, which is characterized by hyperglycemia, obesity, dyslipidemia, hypertension, prothrombic and proinflamatory state, is a risk factor for cardiovascular disease and diabetes mellitus. The synbiotic yogurt of tanduk banana (Musa paradisiaca fa. corniculata) flour contains lactic acid bacteria, fructooligosaccarides, flavonoids, and vitamin C which all of them play role in improving the lipid profile.Objective: The aim of this study was to prove the effect of synbiotic yogurt of tanduk banana flour on lipid profile of metabolic syndrome rats.Method: This study was an true experimental with pre-post test  control group design. The subject of this study were 18 metabolic syndrome male Wistar rats divided into 3 groups,i.e control group (standart diet), intervention group I (standart diet and synbiotic yogurt of banana flour 0,009 ml/g weight/day), and intervention group II (standart diet and synbiotic yogurt of banana flour 0,018 ml/g weight/day). The intervention period was 2 weeks. Different test before and after intervention used paired t-test. The difference test between groups used One-Way ANOVA and Kruskal wallis.Results: The result showed that both of intervention group improved lipid profile significantly (p<0,05). The intervention group II showed more effective improvement of lipid profile significantly (p<0,05) than intervention group I. The intervention group II decreased triglycerides level 41,56%; total cholesterol level 41,39%; LDL-cholesterol level 57,5%; and increased HDL-cholesterol level 139,62%.Conclucion: The intervention of synbiotic yogurt of  tanduk banana flour can decrease triglyceride level, total cholesterol level, LDL-cholesterol level, and increase HDL-cholesterol level.
Pengaruh yogurt sinbiotik pisang terhadap kadar glukosa dan insulin tikus sindrom metabolik Rahmawati, Fiqhi Cahyaningrum; Djamiatun, Kis; Suci, Nyoman
Jurnal Gizi Klinik Indonesia Vol 14, No 1 (2017): Juli
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Original Source | Check in Google Scholar

Abstract

Background: The metabolic syndrome often begins with insulin resistance characterized by hyperinsulinemia and hyperglycemia. Synbiotic yoghurt tanduk banana contains probiotic and prebiotic FOS can improve insulin resistance in metabolic syndrome.Objective: To prove the effect of synbiotic yoghurt tanduk banana can reduce blood glucose levels and insulin levels in metabolic syndrome rats.Method: True-experimental study with randomized controlled group pre-post test design in male wistar rats. Eighteen samples were induced to become metabolic syndrome with high fat fructose diet for 2 weeks and they were divided into 3 groups: control (K) was given standard diet, treatment group 1 (P1), and treatment group 2 (P2) were given sinbiotic yoghurt tanduk banana dose of 0.009 ml/g body weight (BW)/day and 0.018 ml/g BW/day for 2 weeks.Results: The paired t-test showed there were differences of blood glucose levels and insulin levels before and after treatment in P2 group. The Kruskal Wallis test showed there were differences of mean blood glucose levels inter group after treatment (p<0.05). The One-way Anova test showed there were differences of mean insulin levels inter group after treatment (p<0.05). Conclusion: Synbiotic yoghurt tanduk banana can reduce blood glucose levels and insulin levels in metabolic syndrome rat with effective dose is 0.018 ml/grBW/day.
Pengaruh Ekstrak Daun Kersen (Muntingia calabura L) terhadap Kolesterol Darah, Soluble ICAM-1 dan Pembentukan Sel Busa pada Tikus dengan Diet Tinggi Lemak dan Kolesterol Layli, Alvia Nur; Djamiatun, Kis; Kartasurya, Martha Irene
Jurnal Kedokteran Brawijaya Vol 29, No. 3 (2017)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Original Source | Check in Google Scholar | Full PDF (797.425 KB)

Abstract

Ekstrak daun kersen (EDK) terbukti mempunyai aktivitas antioksidan dan anti-inflamasi yang signifikan namun belum pernah dilakukan penelitian EDK terhadap kadar kolesterol dan inflamasi vaskuler. Penelitian ini bertujuan untuk membuktikan pengaruh EDK terhadap kadar kolesterol darah, kadar soluble ICAM-1 dan pembentukan sel busa aorta tikus wistar yang diberi diet tinggi lemak dan kolesterol (DTL-TK). Penelitian dilakukan dengan Post Test-Only Controlled Group Design. Tikus Wistar jantan sebanyak 24 ekor dirandom menjadi 4 kelompok diantaranya kontrol negatif (KN), kontrol positif (KP), EDK dosis 1 (D1), EDK dosis 2 (D2). KN diberi diet normal, KP diberi DTL-TK saja, D1 diberi DTL-TK dan EDK 250mg/kg BB, D2 diberi DTL-TK dan EDK 500mg/kg BB. Pakan dan EDK diberikan 1 kali dalam sehari. EDK diberikan menggunakan sonde, bersamaan dengan pemberian pakan selama 8 minggu. Hasil penelitian menunjukkan bahwa terdapat perbedaan bermakna (p&lt;0,05) antara KN dan KP pada kadar kolesterol HDL. Terdapat perbedaan bermakna (p&lt;0,05) antara KN dan D1 pada kadar kolesterol total. Terdapat perbedaan bermakna (p&lt;0,05) antara KP dan D1 pada kadar soluble ICAM-1. Terdapat perbedaan bermakna (p&lt;0,05) antara KP dan D2 pada kadar kolesterol total, kolesterol HDL, dan kadar soluble ICAM-1. Tidak terdapat perbedaan bermakna (p&gt;0,05) antara D1 dan D2 pada semua parameter. Hasil pengamatan histopatologi aorta abdominal belum menunjukkan pembentukan sel busa diantara 4 kelompok. EDK dosis 500mg/kg BB dapat menurunkan kadar kolesterol total tikus yang diberi DTL-TK, EDK dosis 250mg/kg BB dan 500mg/kg BB dapat menurunkan kadar soluble ICAM-1 tikus yang diberi DTL-TK. Kata Kunci: Daun kersen, kolesterol, soluble ICAM-1, sel busa
Pengaruh pemberian yogurt sinbiotik tepung pisang tanduk terhadap profil lipid tikus sindrom metabolik Octavia, Zana Fitriana; Djamiatun, Kis; Suci, Nyoman
Jurnal Gizi Klinik Indonesia Vol 13, No 4 (2017): April
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Original Source | Check in Google Scholar

Abstract

Background: The metabolic syndrome, which is characterized by hyperglycemia, obesity, dyslipidemia, hypertension, prothrombic and proinflamatory state, is a risk factor for cardiovascular disease and diabetes mellitus. The synbiotic yogurt of tanduk banana (Musa paradisiaca fa. corniculata) flour contains lactic acid bacteria, fructooligosaccarides, flavonoids, and vitamin C which all of them play role in improving the lipid profile.Objective: The aim of this study was to prove the effect of synbiotic yogurt of tanduk banana flour on lipid profile of metabolic syndrome rats.Method: This study was an true experimental with pre-post test  control group design. The subject of this study were 18 metabolic syndrome male Wistar rats divided into 3 groups,i.e control group (standart diet), intervention group I (standart diet and synbiotic yogurt of banana flour 0,009 ml/g weight/day), and intervention group II (standart diet and synbiotic yogurt of banana flour 0,018 ml/g weight/day). The intervention period was 2 weeks. Different test before and after intervention used paired t-test. The difference test between groups used One-Way ANOVA and Kruskal wallis.Results: The result showed that both of intervention group improved lipid profile significantly (p<0,05). The intervention group II showed more effective improvement of lipid profile significantly (p<0,05) than intervention group I. The intervention group II decreased triglycerides level 41,56%; total cholesterol level 41,39%; LDL-cholesterol level 57,5%; and increased HDL-cholesterol level 139,62%.Conclucion: The intervention of synbiotic yogurt of  tanduk banana flour can decrease triglyceride level, total cholesterol level, LDL-cholesterol level, and increase HDL-cholesterol level.
Tepung ulat sagu (Rhyinchophorus ferrugineus) imunomodulator Nitric Oxide (NO) sirkulasi mencit terapi antimalaria standar Ariani, Ariani; Anjani, Gemala; Sofro, Muchlis Achsan Udji; Djamiatun, Kis
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 6, No 2 (2018)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Original Source | Check in Google Scholar | Full PDF (447.96 KB)

Abstract

Background : Sago worm flour (Rhyinchophorus ferrugineus)is a coconut beetle larvae that is processed into flour.This flour contains antioxidants as well as arginine, both of which play a role in modulating oxidative stress including NO involved in immunopathology of cerebral malaria.Objectives : To prove the immunomodulator role of sago worm flour in decreasing circulation  NO level at mice  who received standard antimalarial therapy  Dihydroartemisinin Piperaquine (DHP)Methods : This study was randomized control group post test only design by using 23 Swiss mice which divided into 5 group consist of : K(-) normal mice; K(+) inoculated woth Plasmodium berghei ANKA (PbA); X1 received DHP; X2 received sago worm flour; X3 received both of sago worm flour and DHP. All treatment groups X1,X2 and X3 were inoculated by PbA before treatmen were given. Serum circulation NO level was assessed by ELISA. statistical analysis used was One Way ANOVA followed by Bonferroni post hoc test.Results : The mean  NO level in K(-), K(+), X1, X2, and X3 were 1.008 μmol/mL, K(+) 1.338 μmol/mL; while at treatment X1 1.143 μmol/mL, X2 1.410 μmol/mL, dan X3 0.886 μmol/mL. One way ANOVA showed that they were significantly different (p=0.001). Bonferroni post hoc test of X2 was  proportional to K(+) (p=1.000), whole had NO level lower than either K(+)(p=0.009) or X2 (p=0.002). Conclusion : The immunomodulatory effects of sago worm flour (Rhyinchophorus ferrugineus) reduced circulating NO levels in standard antimalarial therapy recipients.
Pengaruh Ekstrak Etanol Daun Kelor (Moringa oleifera) pada Ekspresi Insulin dan Insulitis Tikus Diabetes Melitus Sulistyorini, Ratna; Sarjadi, -; Johan, Andrew; Djamiatun, Kis
Majalah Kedokteran Bandung Vol 47, No 2 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar | Full PDF (461.161 KB)

Abstract

Kandungan quercetin pada Moringa oleifera secara ilmiah memiliki potensi sebagai antioksidan dan anti-inflamasi dengan menghambat aktivitas NF-ĸB, serta triterpenoid menstimulasi regenerasi sel β pankreas dan meningkatkan sekresi insulin. Penelitian ini bertujuan melihat pengaruh pemberian ekstrak etanol daun Moringa oleifera terhadap insulitis dan ekspresi insulin pankreas tikus Sprague-Dawley jantan. Penelitian dilakukan di laboratorium Unit Pangan dan Gizi, Pusat Antar Universitas Universitas Gadjah Mada serta Laboratorium Histologi dan Biologi Sel Fakultas Kedokteran Universitas Gadjah Mada, periode Mei–Juli 2013. Penelitian ini menggunakan desain randomized post test only controlled group. Hewan coba sebanyak 24 ekor tikus, diperiksa kadar gula darahnya pada hari ketiga setelah induksi streptozotosin, untuk melihat tikus sudah dalam keadaan hiperglikemik. Tikus kemudian  dibagi dalam tiga kelompok: satu kelompok kontrol dan  dua kelompok perlakuan yang  diberi ekstrak etanol daun Moringa oleifera dosis 250 dan 500 mg/kg/hari selama 21 hari. Gambaran histopatologik dinilai  derajat insulitis pada pulau Langerhans dan dinilai ekspresi insulin dengan skor Allred. Uji statistik yang digunakan adalah Kruskal Wallis dilanjutkan Mann-Whitney dengan taraf kemaknaan p<0,05 karena data tidak berdistribusi normal. Derajat insulitis dan ekspresi insulin pankreas tikus pada kedua kelompok perlakuan menunjukkan perbedaan bermakna dibanding dengan kelompok kontrol (p<0,005). Pada penelitian ini dapat disimpulkan bahwa ekstrak etanol daun Moringa oleifera dosis 250 dan 500 mg/kg menyebabkan ekspresi insulin lebih tinggi dan derajat insulitis lebih rendah dibanding dengan kelompok kontrol.[MKB. 2015;47(2):69–76]Kata kunci: Ekspresi insulin, insulitis, Moringa oleifera, streptozotosinEffect of Ethanol Extract of Moringa oleifera Leaves on Insulin Expression and Insulitis in Diabetes Mellitus RatsAbstractQuercetin content in Moringa oleifera has scientific potential antioxidant and anti-inflammation effects by inhibiting the activity of NF-ĸB. In addition, triterpenoids stimulate pancreatic β cell regeneration and increases insulin secretion. This study aimed to discover the effect of  Moringa oleifera extract on insulitis and insulin expression in pancreas of  Sprague-Dawley male rats. The study was performed at the laboratory of Food and Nutrition Unit of The Inter University Center of Gadjah Mada University and the laboratory of Histology and Cell Biology Faculty of Medicine  Gadjah Mada University during May–July 2013. This was a randomized post test only controlled group study. Twenty four rats were included. Blood glucose test was performed on the third day after streptozotocin induction to ensure that all rats were in hyperglycemic condition. The rats were then divided into three groups: one control group and two treatment groups receiving Moringa oleifera ethanolic leaves extract for 21 days in doses of 250 and 500 mg/kg/day, respectively. Histopathological analysis of pancreas were performed by evaluating insulitis  based on the infiltration of mononuclear inflammatory cells to the Langerhans islets. Insulin expression was evaluated using Allred score. Statistical analysis used was Kruskal-Wallis test, followed by  Mann-Whitney  with a level of significance of p<0.05. The insulitis degree and insulin expression of the two treatment groups were significantly  different compared to the control group (p<0.005). It can be concluded that Moringa oleifera ethanolic leaf extract doses of 250 and 500 mg/kg/day cause higher insulin expression and lower  insulitis degree than in the control group. [MKB. 2015;47(2):69–76]Key words: Insulin expression, insulitis, Moringa oleifera, streptozotocin DOI: 10.15395/mkb.v47n2.456