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Perbanyakan Tunas Mikro pada Beberapa Umur Simpan Umbi dan Pembentukan Umbi Mikro Bawang Merah pada Dua Suhu Ruang Kultur Dinarti, Diny; Purwoko, Bambang Sapta; Purwito, Agus; Susila, Anas Dinurrohman
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 2 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Shallot bulb generally stored for several month before planted in the field. Since explant age is one of important factors in tissue culture development, storage period of shallot bulb might alter the explant growth in vitro. Shoots of shallot formed in the in vitro culture should form bulbs before can be use as seedling, and temperature may affect micro bulb induction. Two experiments had been conducted to evaluate 1) the effect of storage period in the field on the growth of shallot explant in vitro and 2) the effect of culture room temperature in microbulbs induction of shallot. In the first experiment, shallot bulb had been stored for 1, 2, 3 and 4 months before used as explants. Storage period significantly influenced the explant growth in vitro. Bulb with 2 months storage gave the best performance on number of micro shoot, number of leaves and roots, and less of vitrification. Micro shoots on three weeks after planting (WAP) was feasible to use as propagule for shallot micro bulb induction. In the second experiment, shoots from propagation medium was transplanted to bulb induction medium and  grown in growth chamber with different temperatures (day/night) 20/17 oC and 30/27 oC, respectively.  Micro bulb induction was influenced by temperature. Lower temperature showed good results for number of leaves, length of leaves, number of roots, and length of roots. However, temperature of 30/27 oC gave the best result on number of micro bulb, diameter of bulb and bulb width:bulb disk diameter ratio.   Keywords: Allium, explants age, micro bulb,  temperature, 2ip
Pengaruh BAP dan Sukrosa terhadap Perbanyakan Jahe Empirit (Zingiber officinale Rosc var. amarun) secara In Vitro Rahmawati, Marai; Aziz, Sandra A.; Dinarti, Diny; Sastra, Dodo R.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 32, No 3 (2004): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

The need to produce numerous and good quality plantlet in short time has been carried out with in vitro culture. The objective of this research was to study BAP and sucrose effect on the in vitro multiplication of small ginger. Research was done in Plant Tissue Culture Laboratory, Department of Agronomy. Bogor Agricultural University from November 2002 until August 2003. The treatment used BAP 0, 1, 2, 3, 4, 5 ppm and sucrose 20, 30, 40, 50 g/l. The result showed that sucrose significantly influenced shoot number in 2-5 and 8 Week After Planting (WAP) and leaf number in 2-7 WAP; root length, root number and explant fresh weight. BAP only significantly influenced leaf number in 7 WAP and root quality. With time shoot color changed from green to yellow. Higher RAP and sucrose concentration increased micro rhizome percentage. Sucrose 40 g/l or RAP 2 ppm gave numerous shoot and high explant fresh weight. Key words : Zingiber officinale Rosc var. amarun, In vitro, BAP, Sucrose
Pengaruh Kombinasi Zat Pengatur Tumbuh BAP dan IAA terhadap Pertumbuhan dan Perkembangan Tanaman Daun Dewa (Gynura procumbens (Back)) dalam Kultur in Vitro Aziz, Sandra Arifin; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 32, No 3 (2004): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

Gynura procumbens (Back) has been used for traditional medical treatment in Indonesia. Micropropagation. one of propagation methods, becomes an effective method in propagating the G. p.rocumbens (Back). The in vitro research using The group Randomize Block Design was conducted to study the growth and development of G. procumbens (Back) explant by applying combinations of BAP ( 0, /,2, and 3 ppm) and IAA (0, 0.5, and / ppm) in the Murashige and Skoog (MS) medium. The results showed combination of BAP 3 ppm and IAA 0.5 ppm was the optimum combination for the shoot multiplication, which produced 85.4 shoots per bottle. BAP 3 ppm combined with / ppm of lAA resulted in 100 % callussed culture with the largest diameter. Key words: Gynura u.rocumbens (Back), BAP, IAA, in Vitro
Induksi Umbi Mikro Tanaman Daun Dewa (Gynura pseudochina (Lour.) DC) Secara In Vitro dengan Perlakuan Sukrosa dan Daminozide Hartanto, Donny; Aziz, Sandra Arifin; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 2 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> The effects of sucrose and daminozide on in-vitro microtuber formation were evaluated for producing microtubers to supply year round microtubers and to facilitate sterilized explants exchange regionally and internationally. Uninodal stem segment explants were cultured on agar solidifi  ed Murashige and Skoog medium supplemented with 3% sucrose, 0.5 mg L-16-benzylaminopurine (BAP), 0.1 mg L-1 α-naphthaleneacetic acid (NAA) for bud multiplication for 3 months. Three nodals stem segment which already  formed  three  perfect leaves were cultured on agar solidifi  ed MS medium, 3% sucrose and 1 mg L-1 NAA for two weeks to induce root formation (until ± minimum 60% of the explants rooted). The next step was the induction of three nodal stem segments for microtuber formation on agar solidifi  ed MS medium supplemented with 5 mg L-1 BAP and two levels of sucrose, four concentrations of daminozide for 10 weeks. Sucrose at 6% resulted in the signifi  cantly highest number of microtuber. The daminozide 41.29 mg L-1 stimulated tuberization at base of the stems and reduce number of microtuber formation in stolon. Keywords: daminozide, Gynura pseudochina (L.) DC, in vitro, microtuber, sucrose
Kultur Jaringan Kantong Semar (Nepenthes mirabilis) Dinarti, Diny; Sayekti, Urip; Alitalia, Yayu
Jurnal Hortikultura Indonesia Vol 1, No 2 (2010): Jurnal Hortikultura Indonesia
Publisher : Perhimpunan Hortikultura Indonesia

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Abstract

This research was about in vitro propagation of Pitcher Plant Nepenthes mirabilis. The aims of this research were to determine: 1) the influence of kind and concentration of in vitro medium and also plant growth regulator on germination of Nepenthes mirabilis, 2) the effect of BAP and NAA on shoot multiplication of N. mirabilis. This research consisted of three experiments and all experiments used  Completely Randomized Design. On the first experiment MS (Murashige and Skoog) and KC (Knudson C) were used, i.e. full (1), three-fourth (3/4), one-half (1/2) and one-fourth (1/4) Salt Concentration. On the second experiment 10 composition media were used, i.e. ½ MS, ½ MS+TDZ 0.01 ppm, ½ MS+IAA 0.1 ppm, ½ MS+GA3 10 ppm, ½ MS+150 ml coconut water, 1/4KC, 1/4 KC+TDZ 0.01 ppm, ¼KC+IAA 0.1 ppm, 1/4KC+GA3 10 ppm and 1/4KC+ 150 ml coconut water.  On the third experiment BAP with 0, 0.5, 1,  2 ppm and NAA 0, 0.1, 0.2, 0.5 ppm were used as factor for shoot multiplication. The result showed that KC medium was the best medium for germination percentage of N. mirabilis (64%).  MS or KC medium with ½ or ¼   salt  concentration was the suitable for  germinating time of   N. mirabilis (average 39 days after showing).  Adding of TDZ, IAA, and GA3 significantly increased germination percentage (70 – 90%)  and decreased germinating time (27 – 38 days after showing).Medium with BAP 0 - 1 ppm was the best for shoot and leave induction and the best medium for root growth of N. mirabilis were MS or KC medium without NAA.   Key words: Nepenthes mirabilis, MS, KC, TDZ, IAA, GA3, BAP, NAA, in vitro propagation, medium
Induksi Tetraploid Tunas Pucuk Jeruk Siam Simadu (Citrus nobilis Lour) Menggunakan Kolkisin secara In Vitro Yulianti, Fitri; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 42, No 2 (2014): Jurnal agronomi indonesia
Publisher : Bogor Agricultural University

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Abstract

ABSTRACT Seedless fruit is one of the criteria necessary to improve the quality of Simadu tangerine. The most effective method to obtain seedless triploid cultivars is hybridisation between tetraploid and diploid parents. Simadu tangerine is diploid plant. Tetraploid Simadu tangerine can be obtained with doubling chromosome using colchicines. The aim of this research was to obtain tetraploid Simadu tangerine shoot which would serve as parent to produced seedless Simadu tangerine. Shoot-tips of Simadu tangerine without leaves were treated with colchicines at four different concentrations (0%, 0.1%, 0.2%, and 0.3%) for 3 hours.  The results showed that the high concentration reduced survival rate. The colchicine treatments reduced growth of shoot-tip of Simadu Tangerine. The leaves of colchicines treated shoots were thicker than control. Leaves from control (0% colchicine) and 0.1% colchicine treated shoots had 8.67 and 18.25 chloroplasts per pair of guard cells. Leaves from 0.1% colchicine treated shoots had lower stomatal density than those of the control (0% colchicine) shoots. Leaves from 0.1% colchicine treated shoots had larger stomatal sizes than those of the control (0% colchicine) shoots. Therefore, it appeared that 0.1% colchicine treatment resulted in tetraploid Simadu Tangerine Shoot.  Keywords: chloroplasts, doubling chromosomes, and stomatal size
Induksi Mutasi Kalus Embriogenik Gandum (Triticum aestivum L.) melalui Iradiasi Sinar Gamma untuk Toleransi Suhu Tinggi Setiawan, Ryan Budi; Khumaida, Nurul; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 43, No 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

Mutation techniques through gamma ray irradiation is useful to support breeding programs for genetic improvement of wheat. Genetic improvement on tolerance to high temperatures is necessary for development of wheat in Indonesia. The purpose of this study was to determine the level of radiosensitivity to be used as the basis for the induction of mutations by gamma ray irradiation on embryogenic callus to obtain putative mutants with high temperature tolerance. Explants used were embryogenic callus cultured on MS medium containing 30 g L-1 sukrosa, 2 g L-1 gelrite, 2 mg L-1 2.4D and 1 mg L-1 picloram. Culture incubated for 6 weeks with temperature 20?4 oC in the room culture. Five irradiation doses (10, 20, 30, 40 and 50 Gy) were used in radiosensitivity testing. A factorial, completely randomized design was applied to the experiment. The first factor was selection temperature with three levels (25, 30 and 35 oC), and the second factor was doses of gamma ray iradiation with three levels (10, 20, and 30 Gy). The result showed that radiosensitivity levels varied among varieties, LD20: 7.79 to 18.96 Gy and LD50: 24.29-33.63 Gy. Selayar variety which has highest sensitivity value compared with Dewata and Nias. Increasing doses of iradiation and temperature decrease survival of embryogenic callus, number of embryos, and percentage of germinated plantlets. Based on in vitro selection using high temperature (25, 30, and 35 oC), the obtained 19 putative mutants were derived from embryos that appear on the surface of embryogenic callus survival after irradiation and high temperature selection.Keywords: in vitro selection, putative mutant, radiosensitivity, somatic embryo
PENGARUH IRADIASI SINAR GAMMA TERHADAP VARIASI PERTUMBUHAN ANGGREK Brachypeza indusiata (Reichb.f) Garay SECARA IN VITRO Wardhani, Maria Utami Dewi; Puspitaningtyas, Dwi Murti; Dinarti, Diny
Buletin Kebun Raya Vol 10, No 2 (2007): Buletin Kebun Raya Vol. 10 (2) Juli 2007
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

A research was carried out in Orchid Laboratory of Bogor Botanical Gardens to observe the effect of gamma ray irradiation on the growth variation of Brachypezo indusiata (Reichb.f) Garray and suitable culture medium for propagating the plant. The research was arranged in a factorial experiment with a Completely Randomized Design. The first factor was gamma ray irradiation on protocorm-like­bodies (plb) of germinated seeds of B. indusiata in which four levels of irradiation was applied, i.e. 0, 10, 20, and 30 Gy. The second factor was the use of two different culture media, i.e. Vacin & Went medium supplemented with either banana pulp or a mixture of tomato and sprout bean as the essential organic compound of the medium. The result showed that the irradiation treatment improved the survival of B. indusiata explants, most significantly at 20 Gy level of irradiation, and could modify the growth of the resulted plantlets by reducing the height of the plant and the length and width of the leaves. The higher the level of irradiation the smaller the plantlets produced. Meanwhile, the addition of organic compound derived from banana fruit into the basal medium of Vacin & Went enhanced the growth of the plantlets better than that derived from a mixture of tomato fruit and bean sprout. However, there was no significant interaction effect detected between the irradiation treatments and the use of different culture media.
Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus Nasution, Siti Shofiya; Dinarti, Diny; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol 13, No 6 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

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Abstract

Infection of Onion yellow dwarf virus (OYDV) are reported causing problems in garlic production. Planting virus-free bulbs might help reduce viral disease incidence in the field. This research was aimed to develop method for eliminating OYDV from garlic bulbs using combination of electrotherapy (0, 5, 10, 15, and 20 mA each for 10 minutes) and thermotherapy (23, 28, 33, 38?C each for 4 weeks). Two garlic cultivars, i.e. Sangga Sembalun and Lumbu Hijau were used as seed bulbs for OYDV elimination tests. Virus infection was confirmed using transcription-polymerase chain reaction (RT-PCR).? The result showed that thermotherapy at 33 ?Cwas the best method to eliminate OYDV in garlic although the efficiency was not the same for all cultivars. The efficiency reached up to 60% for cv. Lumbu Hijau, whereas for cv. Sangga Sembalun only reached up to 40%. Electrotherapy alone or in combination with thermotherapy were not able to produce OYDV-free plantlets.
PERTUMBUHAN DAN HASIL BAWANG MERAH ASAL UMBI TSS VARIETAS TUK TUK PADA UKURAN DAN JARAK TANAM YANG BERBEDA Darma, Wika Anrya; Susila, Anas Dinurrohman; Dinarti, Diny
Agrovigor 2015: Vol 8, No 2 (2015) SEPTEMBER
Publisher : Trunojoyo University of Madura

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Abstract

Penelitian ini dilaksanakan mulai Oktober 2014 hingga Februari 2015 di Green House Kebun Percobaan IPB Cikabayan, Dramaga, Bogor. Penelitian ini bertujuan memperoleh ukuran umbi asal TSS dan jarak tanam yang optimal dalam memproduksi umbi benih bawang merah. Rancangan penelitian menggunakan Rancangan Acak Lengkap dua faktor. Faktor pertama yaitu ukuran umbi yang terdiri dari umbi berukuran sedang (diameter= 1.5-1.8 atau berbobot 5-10 g) dan besar (diameter= >1.8 cm atau berbobot >10 g). Faktor kedua yaitu jarak tanam yang terdiri dari jarak tanam 20x20 cm dan 30x30 cm. Umbi yang digunakan dalam percobaan ini merupakan umbi hasil penanaman dari biji TSS yang telah disimpan selama dua bulan. Hasil penelitian menunjukkan bahwa penggunaan umbi berukuran sedang dan besar tidak memberikan pengaruh yang nyata terhadap produksi bawang merah. Sedangkan jarak tanam memberikan hasil yang berbeda nyata. Penggunaan umbi berukuran sedang pada jarak tanam yang lebih rapat dapat meminimalkan biaya produksi dari segi penyediaan umbi benih..Kata kunci: Bawang merah, jarak tanam, ukuran umbi, umbi benih asal TSS