Maruni Wiwin Diarti
Health Analysts Department, Polytechnic Health Ministry of Semarang

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The Result Discrepancies between Histological and PCR Method for Detecting Helicobacter pylori in Patients with Dyspepsia due to Inappropriate Preparation before Endoscopy

The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 10, ISSUE 2, August 2009
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: False negative result of Helicobacter pylori (H. pylori) detection in gastric tissue can be due to inappropriate preparation before endoscopy. The objectives of this study is to compare the result of H. pylori detection in gastric biopsy by histological method and ure C polymerase chain reaction (PCR) in patients with dyspepsia who underwent upper gastrointestinal (GI) endoscopy without preparations other than six hours fasting before endoscopy. Method: We obtained 156 paraffin blocks of gastric endoscopic biopsy samples, taken from antrum and corpus of patients with dyspepsia who underwent endoscopic examination at the Endoscopy Unit of Biomedika hospital, Mataram. All biopsy samples were stained with Hematoxylin and Eosin for tissue diagnosis and Giemsa stain for detecting H. pylori Ure C PCR were done on all blocks. Cag PCR were performed on all Ure C PCR positive samples. Results: Of 156 paraffin blocks, only 17 blocks (10.9%) were positive for H. pylori by histological examination. All of the 17 samples showed positive results on PCR method. Of 156 paraffin blocks, positive results were found in 73 patients (45.9%) by ure C PCR method. The PCR method has increased the positivity rates of H. pylori more than four times compared to histological method. This study showed that the rate of cag a was 63.0%. Conclusion: Ure C PCR is superior to histological examination in patients who did not stop consuming acid supressor drug and antibiotic two weeks prior to endoscopy. This phenomenon can be explained by the change of spiral form into coccoid form of H. pylori, which is hardly detected using Giemsa stain.   Keywords: Helicobacter pylori, histology, ureC, Cag a, PCR

The Result Discrepancies between Histological and PCR Method for Detecting Helicobacter pylori in Patients with Dyspepsia due to Inappropriate Preparation before Endoscopy

The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 10, ISSUE 2, August 2009
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: False negative result of Helicobacter pylori (H. pylori) detection in gastric tissue can be due to inappropriate preparation before endoscopy. The objectives of this study is to compare the result of H. pylori detection in gastric biopsy by histological method and ure C polymerase chain reaction (PCR) in patients with dyspepsia who underwent upper gastrointestinal (GI) endoscopy without preparations other than six hours fasting before endoscopy. Method: We obtained 156 paraffin blocks of gastric endoscopic biopsy samples, taken from antrum and corpus of patients with dyspepsia who underwent endoscopic examination at the Endoscopy Unit of Biomedika hospital, Mataram. All biopsy samples were stained with Hematoxylin and Eosin for tissue diagnosis and Giemsa stain for detecting H. pylori Ure C PCR were done on all blocks. Cag PCR were performed on all Ure C PCR positive samples. Results: Of 156 paraffin blocks, only 17 blocks (10.9%) were positive for H. pylori by histological examination. All of the 17 samples showed positive results on PCR method. Of 156 paraffin blocks, positive results were found in 73 patients (45.9%) by ure C PCR method. The PCR method has increased the positivity rates of H. pylori more than four times compared to histological method. This study showed that the rate of cag a was 63.0%. Conclusion: Ure C PCR is superior to histological examination in patients who did not stop consuming acid supressor drug and antibiotic two weeks prior to endoscopy. This phenomenon can be explained by the change of spiral form into coccoid form of H. pylori, which is hardly detected using Giemsa stain.   Keywords: Helicobacter pylori, histology, ureC, Cag a, PCR

Been soursop Extract Against Larvae of Aedes sp Killing Activity

Jurnal Riset Kesehatan Vol 2, No 2 (2013): Mei 2013
Publisher : UPPM Politeknik Kesehatan Kementrian Kesehatan Semarang

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Abstract

The aim of this studyis to determine thedose of been soursop extract against larvae of Aedes sp killing activity (larvacida) that derived from dengue endemic are asin Mataram with bioas say testingin the laboratory.The research methodused wascompletely randomized experimental design. The samples used wereinstar III oflarvaeAedesspderived from eggsof Aedes sp mosquitoes that were collectedin the homes of residentsin endemic areas dengue feverin the city of Mataram.Results of bioas say testin the laboratory was obtained D56 and LD90 are 0.14 ppm and 0.20 ppm respectively. So that, the been of soursop extract is effective tokill the larvaeof Aedes sp.

SENSITIVITAS MEDIA OGAWA DAN MEDIA LOWENSTEIN JENSEN TERHADAP HASIL PERTUMBUHAN KUMAN MYCOBACTERIUM TUBERCULOSIS

Jurnal Kesehatan Prima Vol 8, No 2 (2014): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract: Referring to the national TB control program, the diagnosis made by microscopic examination of sputum by direct and definitive diagnosis by culture examination. Direct sputum examination is the gold standard method of microscopic examination of a recognized WHO TB and confirmation done by the method of culture. Research "Sensitivity Test Using Ogawa Medium and Lowenstein Jensen Medium Againts Micobacterium tuberculosis Bacteria Growth Results ", performed to identify and compare the results of Ogawa medium and culture on Lowenstein-Jensen medium and analyze the sensitivity between the two medium. A descriptive observational study was conducted. Study sample is a sample saturated with a consecutive sampling. Sample size retrieval technique based on sample collection time, which is June 8 through July 18, 2011, a total of 53 samples. Research is to test the sensitivity variables as independent variables and the growth of Mycobacterium tuberculosis bacteria on the Ogawa medium and LJ medium as a bound variable. The results of the study, of 53 samples, 21 (39.6%) samples were negative, so that 32 (60.4%) samples tested positive for M tuberculosis and the total growth of 36 samples (67.9%). Lowenstein Jensen (LJ) medium is more sensitive than the Ogawa medium, both in terms of speed of growth and in the number of colonies that grow. For the determination of Mycobacterium species needs to be done other tests to confirm. Culture results can also be used for comparison using the PCR method, OAT resistance by culture methods.

KADAR ENZIM KHOLINESTERASE DARAH PETANI TERPAPAR PESTISIDA YANG DIBERIKAN RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb)

Jurnal Kesehatan Prima Vol 9, No 2 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract: This study aims to determine the potential use of ginger rhizome (Curcuma xanthorrhiza Roxb) in increasing levels of enzyme kholinesterase on farmers who are exposed to toxic pesticides.The research method used is pre - posttest Control Design. In this research, there are 3 groups of samples each of 30 persons, ie negative control group who did not experience organophosphate and carbamate pesticide poisoning, the positive control group, farmers who suffered pesticide poisoning organophosphates and carbamates but not given the rhizomes of ginger (Curcuma Roxb xanthorrhiza), and treatment groups, farmers who experienced pesticide poisoning were organophosphates and carbamates and ginger rhizome (Curcuma xanthorrhiza Roxb.) Given dose of ginger is 3 grams / day for 9 days. Measurement of blood cholinesterase enzyme levels using tintometer kit. The results are classified on toxin a severe, moderate, mild and not toxin. Result were tested with Wilcoxon Signed Ranks test and Mann-Whitney Test, with 95% confidence level (p 0.05). The research proves that there are significant differences in levels of blood cholinesterase enzyme treatment groups before and after treatment (p 0.05) and no significant differences in levels of blood cholinesterase enzyme treatment group and positive control groups after the study (p <0.05). The conclusion of this study, administration of ginger rhizome (Curcuma Roxb xanthorrhiza) with a dose of 3 grams / day for 9 days can significantly increase levels of plasma cholinesterase enzyme in the farmers who suffered organophosphate pesticide poisoning and Carbamat. Therefore, the researchers recommend that farmers consume a dose of ginger to prevent and reduce organophoshate pesticide poisoning and Carbamat.

TOTAL CHOLESTEROL LEVELS IN TRADITIONAL COFFEE DRINKERS DUSUN SEMBUNG DAYE NARMADA LOMBOK DISTRICT WEST

Jurnal Kesehatan Prima Vol 10, No 1 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract : Traditional coffee is one of the widely-consumed beverages Lombok Island community, one in Hamlet Sembung Daye. Coffee contains cafestol and kahweol substances that can raise cholesterol levels in the blood. This research is a descriptive study that aims to describe how the total cholesterol levels in the traditional coffee drinkers in the hamlet Sembung Daye Narmada District of West Lombok regency that the examination carried out using test methods strip. Data collected in the form of total cholesterol at 83 traditional coffee drinkers in the hamlet Sembung Daye Subdistrict Naramada West Lombok regency. The results showed the average total cholesterol level in males was 225 mg / dl and in women is 217 mg / dl. The conclusion of this study is total cholesterol levels in the traditional coffee drinkers in the hamlet Sembung Daye Narmada District of West Lombok district is more than the normal value.

SENYAWA PENGKUPLING α-NAFTHILAMIN UNTUK VALIDASI METODE SPEKTROFOTOMETRI PENENTUAN NITRIT (〖〖NO〗_2〗^-) DI DALAM AIR

Jurnal Kesehatan Prima Vol 9, No 1 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract: Validation of analytical methods need to be conducted to determine the feasibility of the method when the method is newly developed or modified. Determination of nitrite in water-based Cupling diazotized reaction has been developed in various fields. Many modifications have been carried out primarily in the selection of the source of the diazonium salt compound or compounds that can cupling.  The this study was to determine the validity of the determination of nitrite in water by spectrophotometric  method  cupling  compound α-nafthilamin. This study is a pre-experiments with experimental units and large units based guidelines experimental validation of analytical methods. The results show the value of 0.995 linearity, limit of detection (LOD) 0.039 ppm, 0.882 sensitivity, precision 1.38%, and 100.37% accuracy rate. The conclusion of this study is the method of spectrophotometric determination of nitrite in water using compound α-nafthilamin cupling  valid for determining the concentration of nitrite in the water with a minimum detection limit of 0.039 ppm.

THE ALTERNATIVE DILUTE SOLUTION OF NaCl 0.9% AT THE GIEMSA STAINING ON THE INVESTIGATION THE MORPHOLOGY OF SPERMATOZOA

Jurnal Kesehatan Prima Vol 10, No 2 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract : The quality of staining, the morphology of spermatozoon applays The Giemsa dye, is strongly influenced by the type of Giemsa thinner. The terms of diluent that can be used is having a buffer property. The solution of NaCl 0.9 %, besides having buffer properties also has a cheaper price. The research is a descriptive study that aims to determine NaCl 0.9% as the alternative of diluting solution at Giemsa staining on morphological examination of spermatozoon. This study was conducted to compare the quality of spermatozoon morphology staining by using Giemsa which was diluted with 0.9% NaCl solution and the solution of Phosphate Buffer. The result of the study indicated The Giemsa staining with NaCl 0.9% solution, is obtained the outstanding spermatozoon morphology staining absorption was 71.625% and the poor spermatozoon morphology staining absorption was 28.375%. The results of study which was applied the Phosphate Buffer solution, was obtained the outstanding spermatozoon morphology staining absorption was 81.563% and the poor spermatozoon morphology staining absorption was 18.438%. The NaCL 0.9% can be used as an alternative dilute solution at Giemsa staining on morphological examination of spermatozoon.

ANALISIS MOLEKULER MDR TB DENGAN TEKNIK SEKUENSING DARI SAMPEL DAHAK SUSPEK TB DI NUSA TENGGARA BARAT

Jurnal Kesehatan Prima Vol 9, No 1 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract: Background: Tuberculosis is one of the tropical diseases that are still of concern to the world , especially with the increasing number of developing multidrug- resistant TB variants. In this study we indetified nucleotide sequence MDR-Tb resistant rifampicin, etambhutol dan isoniazid  from  patient sputum who have suspect tuberculosis infection in West Nusa Tenggara. Methods: This analyze with PCR, sequencing and analysis using Blast Basic Local Alignment Search Tool) from NCBI (National Centre of Biotechnology Information). Result : Twelve samples has been obtained in this study, 9 is positive PCR with TB primer and all of them is still sensitive with rifampicin, etambuthol and isoniazid. Ten sequence sensitive from rpoB, emB and katG has sequencing and Blast analysis, sequence rpoB from TB 5 and TB 6 have similarity with sequence strain M.tb CCDC5079 and TB8 and TB 9 with sequence strain M.tb 96121. emB sequence from sample TB8 and TB9 have similarity with M.tb subsp.tuberculosis partial embB putative arabinosyltransferase mutan 240 exon 1; and TB9 with M.tb strain  2a putative arabinosyltransferase. Sequence from katG from TB8,TB9,TB10 have similarity with M.tb strain 96075. Conclution: MDR-Tb is not found in the studies, with Blast analysis we concluded the Mycobacterium tuberculosis sensitive tuberculosis drugs  sequence from sample study have similarity with M.tb Beijing dan manila Family.

TOTAL KOLESTEROL CONDITIONS ON CONSUMPTION OF BLACK POWDER COFFEE (Traditional) WITH PATIENT PREPARATION

Jurnal Kesehatan Prima Vol 12, No 1 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

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Abstract

Abstract: A traditional black coffee powder is one of the beverages that excessively consumed by communities. The increasing of total cholesterol level in blood is influenced by the habit of consuming coffee. Coffee has chemicals that can cause some health problems, namely cafestol and kahweol. The objective study was to determine the effect of patient preparation against the result of total cholesterol levels examination on the consumers of traditional black coffee powder. This research was analytic observational. The sample was capillary blood of consumers’ a traditional black coffee as many as 30 people. Statistical analysis utilized Mann-Whitney U Test with 95% confidence level (α = 0,05). The findings indicated the average deviation of total cholesterol level after fasting 12,86 mg / dL and mean difference after giving coffee 7 cups 18,46 mg / dL. Mann Whitney Test found significant value 0.001 <α = 0.05 which means there is an influence of patient preparation against the result of total cholesterol levels examination on the consumers of traditional black coffee powder.Keywords: Total Cholesterol Level; Consumers of Traditional Black Coffee Powder.