Diah Dhianawaty
Fakultas Kedokteran, Universitas Padjadjaran

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Efek Antihiperkolesterol Jus Buah Belimbing Wuluh (Averhoa bilimbi L.) terhadap Mencit Galur Swiss Webster Hiperkolesterolemia

Majalah Kedokteran Bandung Vol 45, No 2 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Kadar kolesterol darah yang melebihi batas normal merupakan pemicu bermacam penyakit. Tujuan penelitian untuk mengetahui efek antihiperkolesterol jus buah belimbing wuluh, dilakukan pada bulan Maret–Juni 2011 di Sekolah Farmasi Institut Teknologi Bandung. Mencit galur Swiss Webster dibagi menjadi dua kelompok yaitu pencegahan dan pengobatan, tiap kelompok dibagi menjadi empat subkelompok: kontrol negatif hidup normal, tiga subkelompok lainnya kontrol positif, pembanding dan uji (belimbing) diberi pakan tinggi kolesterol dan propiltiourasil 0,26 mg/20 gBB/hari. Pada ketiga subkelompok pencegahan perlakuan di atas disertai pemberian berturut-turut 20 mg tragakan, simvastatin 0,026 mg/20 gBB/hari, dan 0,12 g jus belimbing/20 gBB/hari selama 30 hari. Untuk kelompok pengobatan setelah 30 hari ketiga subkelompoknya diberi tragakan, simvastatin, dan jus belimbing seperti subkelompok pencegahan selama 30 hari. Kadar kolesterol diukur dengan spektrofotometer dan diuji dengan analysis of variance (ANOVA) dilanjutkan dengan uji student-t. Pada kelompok pencegahan perbedaan kadar kolesterol subkelompok kontrol positif 158,5 mg/dL dan belimbing 129,7 mg/dL bermakna (p<0,01), pada kelompok pengobatan tidak bermakna. Simpulan, jus belimbing wuluh dapat mencegah peningkatan kadar kolesterol tetapi tidak dapat mengobati. [MKB. 2013;45(2):125–9]Kata kunci: Antihiperkolesterolemia, Averhoa bilimbi L., belimbing wuluhAnti-hypercholesterol Effect of Averhoa bilimbi L. Fruit Juice on Swiss Webster Strain Mice HypercholesterolemiaBlood cholesterol level which were higher than normal is a trigger of various diseases. The objective of the research was to know the anti-hypercholesterol effect of Averhoa bilimbi L. fruit juice, done in March–June 2011 at School of Pharmacy Institute Technology of Bandung. Webster strain mice were divided into two groups there were preventive and curative. Each group was divided into four subgroups: negative control was normal life, the three subgroups other positive control, reference and test (Averhoa) received high-cholesterol meal and 0.26 mg/20 gBW/day of prophylthiouracyl. At preventive group the treatment above was participated with 20 mg tragacanth, 0.026 mg/20 gBW/day simvastatin, and 0.12 g juice/20 gBW/day respectively during 30 days. For curative group 30 days later the three subgroups other received tragacanth, simvastatin, and juice as subgroups of preventive group during 30 days. The cholesterol levels were measured with spectrophotometer and were examined with analysis of variance (ANOVA) and were continued with student-t test. At preventive group the different of cholesterol level between subgroup control positive 158.5 mg/dL and Averhoa 129.7 mg/dL was significant p<0.01, at curative group were not significant. In conclusion, Averhoa fruit juice can prevent increasing of cholesterol level, but cannot cure. [MKB. 2013;45(2):125–9]Key words: Anti-hypercholesterolemia, Averhoa bilimbi L., small carambola DOI: http://dx.doi.org/10.15395/mkb.v45n2.113

Metode Cepat Identifikasi Flavonoid dari Daun Ocimum sanctum L. (Selasih)

Majalah Kedokteran Bandung Vol 44, No 1 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Manfaat suatu tumbuhan sebagai obat herbal diperoleh dari kandungan kimianya, misalnya flavonoid. Flavonoid bermanfaat untuk kesehatan tubuh manusia. Oleh karena itu, kandungan flavonoid dapat dijadikan sebagai acuan manfaat suatu tanaman dan dibutuhkan metode identifikasi flavonoid yang cepat. Tujuan penelitian untuk memperoleh metode cepat kandungan flavonoid dalam Ocimum sanctum. Telah dilakukan penelitian ekstraksi, isolasi, dan identifikasi flavonoid daun Ocimum sanctum di laboratorium Kimia Medik Fakultas Kedokteran Universitas Padjadjaran pada tahun 2005. Flavonoid diekstraksi sinambung dengan etanol 95%, diisolasi dengan kromatografi kertas/lapis tipis selulose dan asam asetat 2%, kemudian dilanjutkan dengan pengembang: n-butanol–asam asetat–air, kloroform–asam asetat–air, forestall, asam asetat 5%, 15%, 35%, dan 50%. Flavonoid diidentifikasi dengan pereaksi geser dan sinar ultraviolet. Hasil isolasi dengan asam asetat 2% dan 35% berturut-turut memberikan dua bercak flavonoid, sebagai flavon, FOAc-1 mempunyai Rf=0,69 dan FOAc-2 mempunyai Rf=0,57. Pengembang lainnya memberikan satu bercak flavonoid. Simpulan, isolasi dengan asam asetat 2% dan 35% berturut-turut serta identifikasi dengan pereaksi geser dan sinar ultraviolet merupakan metode yang cepat untuk identifikasi kandungan flavonoid dalam Ocimum sanctum. [MKB. 2012;44(1):32–7]. Kata kunci: Flavonoid, identifikasi flavonoid, Ocimum sanctumRapid Identification Method of Flavonoid from Ocimum sanctum L. (Selasih) Leaves A plant’s effectiveness as a herbal drug comes from its chemical content such as flavonoids. Flavonoids are useful for human body health. Therefore flavonoids content can be used as a marker from the usefulness of a plant, and rapid identification method of flavonoid is needed. The objective of the research was to get a rapid method of flavonoid content identification from Ocimum sanctum. The extraction, isolation and identification of flavonoids from Ocimum sanctum leaves has been done at Medical Chemistry laboratory Faculty of Medicine Universitas Padjadjaran in 2005. Flavonoids were extracted continually by 95% ethanol, were isolated with paper chromatography/cellulose TLC and 2% acetic acid, then continued with elucidation reagents: n-butanol–acetic acid–water, chloroform–acetic acid–water, forestall, 5%, 15%, 35% and 50% acetic acids. Flavonoids were identified with diagnostic reagents and ultraviolet light. Isolation with 2% and 35% acetic acids respectively gave two spots of flavonoids as flavon, FOAc-1 had Rf=0.69 and FOAc-2 had Rf=0.57. The other elucidation reagents gave one spot of flavonoid. In conclusion, isolation with 2% and 35% acetic acids respectively and identification with diagnostic reagent and ultraviolet light is a rapid method for identification of flavonoids content in Ocimum sanctum. [MKB. 2012;44(1):32–7].Key words: Flavonoid, identification of flavonoid, Ocimum sanctum DOI: http://dx.doi.org/10.15395/mkb.v44n1.73

Penurunan Aktivitas Tirosinase dan Jumlah Melanin oleh Fraksi Etil Asetat Buah Malaka (Phyllantus emblica) pada Mouse Melanoma B16 Cell-Line

Majalah Kedokteran Bandung Vol 45, No 2 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Akumulasi melanin menyebabkan hiperpigmentasi dan bila terdapat pada wajah dapat menimbulkan masalah psikososial. Bahan depigmentasi dari tanaman semakin banyak digunakan. Bahan yang dikembangkan harus efektif menghambat sintesis melanin serta tidak toksik terhadap melanosit. Penelitian ini bertujuan menguji efek fraksi etil asetat buah Phyllanthus emblica (P. emblica) atau buah malaka, terhadap sintesis melanin dengan mengukur jumlah melanin dan aktivitas tirosinase, yang merupakan enzim utama sintesis melanin, secara spektrofotometrik pada kultur sel melanosit mouse melanoma B16 cell-line. Sitotoksisitas diukur dengan metode 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Penelitian dilakukan pada November–Desember 2009 di Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, Korea Selatan. Hasil penelitian menunjukkan aktivitas tirosinase dan jumlah melanin menurun bergantung pada dosis perlakuan berbagai konsentrasi fraksi etil asetat buah P. emblica dengan inhibition concentration (IC) 50 berturutturut 95,63 dan 16,90 μg/mL, serta lethal dose (LD) 50 pada konsentrasi 106,64 μg/mL. Simpulan, fraksi etil asetat buah P. emblica mempunyai potensi sebagai bahan depigmentasi, karena mampu menurunkan sintesis melanin melalui inhibisi aktivitas tirosinase. [MKB. 2013;45(2):118–24]Kata kunci: Aktivitas tirosinase, etil asetat, melanin, Phyllanthus emblicaReduction of Tyrosinase Activity and Melanin Amount by Ethyl Acetate Fraction from Malaka (Phyllanthus emblica) Fruit in Mouse Melanoma B16 Cell-LineMelanin accumulation can lead to hyperpigmentation, and if it occurs on the face can cause psychosocial problem. Depigmenting agents derived from plants are increasingly utilized. Agents being developed have to be effective in inhibiting melanin synthesis and should not be toxic to melanocyte. This study aimed was to examine the effect of ethyl acetate fraction from Phyllanthus emblica (P. emblica) fruit, also known as malaka fruit, towards melanine synthesis, which was measured from the melanin amount and tyrosinase activity, the key regulatory enzyme in melanin synthesis, spectrophotometrically towards melanocytes of mouse melanoma B16 cell-line. The cytotoxic effect towards melanocytes was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November−December 2009 in Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study showed that tyrosinase activity and melanin amount decreased in a dose-dependent manner towards various concentrations of ethyl acetate fraction of P. emblica fruit with inhibition concentration (IC) 50=95.63 and 16.90 μg/mL, respectively and lethal dose (LD) 50 concentration 106.64 μg/mL. In conclusion, ethyl acetate fraction of P. emblica fruit is a potential depigmenting agent, since it can reduce melanin synthesis by inhibition of tyrosinase activity. [MKB. 2013;45(2):119–24]Key words: Ethyl acetate, melanin, Phyllanthus emblica, tyrosinase activity DOI: http://dx.doi.org/10.15395/mkb.v45n2.115

Larvacidal Effect of Imperata Cylindrical Root Decoction against Culex sp. Larvae

Althea Medical Journal Vol 1, No 2 (2014)
Publisher : Althea Medical Journal

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Abstract

Background: Filariasis is one of the neglected tropical diseases. About 337 of 401 districts in Indonesia are endemic areas for filariasis, especially in Sumatera,  Kalimantan, and Papua. Culex sp. is one of the lymphatic filariasis vectors which can be controlled by insecticide, including larvacide. This study was conducted to determine the larvacidal effect of Imperata cylindrical root decoction against Culex sp. larvae.Methods: This study was conducted at the Laboratory of Parasitology, Faculty of Medicine, Universitas Padjadjaran during the period of September to November 2012. The study object was Culex sp. larvae III/IV instars. The design of this study was experimental laboratory using true experimental approach. The larvae were divided into three groups: negative control (distilled water), reference (Abate®), and decoction. The number of larvae in each group was 25 larvae, and the effects were evaluated by the total number of dead larvae in 48 hours under observation. The data were then analyzed by Mann-Whitney test and Probit test.Results: The result of the Mann-Whitney test to compare Imperata cylindrica root decoction treatment to distilled water as control  was significant (p<0.05). However, Abate® gave a better result. The Probit test resultwas LC50:  63% and LC90: 489%.Conclusions: Imperata cylindrical root decoction has a larvacidal effect against Culex sp. larvae.Keywords: Culex sp., Filariasis, Imperata cylindrica (L.) Beauv., Larvacidal, LC50  Pengaruh Larvasida Rebusan akar alang-alang (Imperata Cylindrical) terhadap Larva Nyamuk (Culex sp.) AbstrakLatar Belakang: Filariasis merupakan salah satu penyakit tropis yang terabaikan. Sekitar 337 dari 401 kabupaten di Indonesia adalah daerah endemik untuk filariasis terutama di Sumatera, Kalimantan, dan Papua.  Nyamuk (Culex Sp.) adalah salah satu vektor filariasis limfatik yang dapat dikendalikan oleh insektisida, termasuk larvasida. Penelitian ini dilakukan untuk mengetahui pengaruh larvasida dari rebusan  akar alang-alang terhadap larva nyamuk (Culex sp.)Metode: Penelitian ini dilakukan di Laboratorium Parasitologi, Fakultas Kedokteran, Universitas Padjadjaran selama periode September-November 2012. Objek penelitian adalah larva nyamuk (Culex sp.) instar III / IV.. Desain penelitian ini adalah eksperimental laboratoris dengan menggunakan pendekatan eksperimental yang benar. Larva dibagi menjadi tiga kelompok: kontrol negatif (air suling), referensi (Abate®), dan rebusan. Jumlah larva dalam setiap kelompok adalah 25 larva, dan efek dievaluasi dengan jumlah total larva mati dalam 48 jam di bawah pengawasan. Data kemudian dianalisis dengan uji Mann-Whitney dan uji Probit. Hasil: Hasil uji Mann-Whitney untuk membandingkan pemberian rebusan akar alang-alang (Imperata cylindrica) terhadap air suling sebagai kontrol adalah signifikan (p <0,05). Namun, Abate® memberikan hasil yang lebih baik. Hasil tes Probit adalah LC50: 63% dan LC90: 489%.Simpulan: Rebusan akar alang-alang (Imperata cylindrical) memiliki efek larvsidal terhadap larva nyamuk (Culex sp.) Kata kunci: Nyamuk (Culex sp.), Filariasis, Alang-alang (Imperata cylindrica (L.) Beauv), Larvasida, LC50 DOI: 10.15850/amj.v1n2.349

Effects of Ageratum conyzoides Leaf Decoction on Glucose Absorption in Rat’s Small Intestine

Althea Medical Journal Vol 5, No 3 (2018)
Publisher : Althea Medical Journal

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Abstract

Background: Obesity is one of the health problems caused by excessive glucose intake which exceeds normal body requirement. To prevent health problems, Indonesian natives used traditional herbs, such as Ageratum conyzoides (billygoat weeds). The study aimed to discover the effect of Ageratum conyzoides leaf decoction on glucose absorption in the small intestine of rats.Methods: An experimental study was conducted at the Biochemical Laboratory of Faculty of Medicine, Universitas Padjadjaran. Two groups of six rats were given Ageratum conyzoides leaf decoction with dosages 0.89 and 1.33 g/kgbw respectively. Each rat was given glucose solution as a control, decoction administration along with the glucose solution, and glucose solution again to discover the effect of decoction in the absorption function after the administration of decoction was discontinued. Glucose absorbed by the intestine was measured every 15 minutes in 1 hour after the administrationof the solution.Results: Glucose absorption was impeded in dosage 1 and 2 with a mean of the gap between glucose absorbed concentration before given decoction and after given decoction were 73.97 and 45.09 mg/dl respectively. Analysis using paired t-test resulted in insignificant meaning (p-value of dosage 0.89 g/kgbw=0.08; p-value of dosage 1.33 g/kgbw=0.06). In the last glucose perfusion, absorption increased in both dosages after administration of decoction was discontinued.Conclusions: Ageratum conyzoides leaf decoctioncan inhibit the glucose absorption in the small intestine but is not statistically significant. Further investigation may be needed  by using a higher concentration of Ageratum conyzoides and/or other filtration methods.

Larvicidal Effects of Tithonia diversifolia (Hemsley) A. Gray Leaf Water Extract against Culex sp. Larvae

Althea Medical Journal Vol 2, No 1 (2015)
Publisher : Althea Medical Journal

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Abstract

  Background: There are various vector mosquitoes that cause filariasis such as Culex sp. Controlling the number of vectors with larvacide can reduce the spread of this disease. The extracts of Tithonia diversifolia (Hemsley) A. Gray leaves are known to have larvicidal effects on Aedes aegypti larvae. The purpose of this study is to determine the larvicidal effects of Tithonia diversifolia (Hemsley) A. Gray leaf water extract against Culex larvae.Methods: This was an experimental study conducted during the period of October to November 2012 at the Laboratory of Biochemistry, Faculty of Medicine, Universitas Padjadjaran. First trials were conducted to find the most effective larvicidal concentration of Tithonia diversifolia (Hemsley) A. Gray leaf water extract in killing 25 Culex larvae. A concentration of 100 ml was decided as the testing concentration which would be compared to a positive control containing Abate® and a negative control containing distilled water. The number of dead larvae was calculated every hour for 48 hours.Results: Effective larvicidal concentration of Tithonia diversifolia (Hemsley) A. Gray leaf water extract was 60% (p=0.000 compared to negative control), which was lower than Abate®. Result also showed LC50 and LC90 of 24% and 42%, respectively.Conclusions: Tithonia diversifolia (Hemsley) A. Gray leaf water extract has a larvicidal effect on  Culex sp. larvae, although it is not as effective as Abate®, Hence, this leaf may be used as an alternative larvacide as these plants are easily available in the community and inexpensive.Keywords: Culex sp., filariasis, larvacide, Tithonia diversifolia (Hemsley) A. GrayDOI: 10.15850/amj.v2n1.441

Oropharyngeal Colonization by Streptococcus pneumoniae among Medical Students in Indonesia

Althea Medical Journal Vol 3, No 3 (2016)
Publisher : Althea Medical Journal

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Abstract

Background: Streptococcus pneumoniae may colonize the upper respiratory tract without causing any symptoms. Medical students may be inhabited by these bacteria and transmit them to patients who were prone to infections. Streptococcus pneumoniae resistance to antibiotics was recently reported. This study was conducted to determine whether there was Streptococcus pneumoniae colonization among Medical Students of the Faculty of Medicine Universitas Padjadjaran Batch 2011 and analyze its susceptibility patterns towards several antibiotics.Methods: A descriptive study was conducted involving 75 Medical Students of the Faculty of Medicine Universitas Padjadjaran Batch 2011 that met the selection criteria. After informed consent, oropharyngeal throat swab was taken and further identification was carried out. Once Streptococcus pneumoniae colony was identified, susceptibility testing would be performed.Results: The identification results indicate that 7 students (9%) were colonized by Streptococcus pneumoniae. The susceptibility test showed that out of 7 isolates, 2 were resistant to 1 antibiotic, 1 was resistant to 2 antibiotics, and 4 were resistant to 3 antibiotics. Meanwhile, Streptococcus pneumoniae was resistant to trimethoprim-sulfamethoxazole (71%), oxacillin (71%), erythromycin (57%), and levofloxacin (14%).Conclusions: Streptococcus pneumoniae colonization is found among medical students. All Streptococcus pneumoniae are resistant to one or more antibiotics, mostly to trimethoprim-sulfamethoxazole and oxacillin. [AMJ.2016;3(3):349–54] DOI: 10.15850/amj.v3n3.878 

NR2B Gene Expression Change in Wistar Rat Practicing Aerobic Exercise Comparing to Soybean (Glycine max) or Phyllanthus niruri Intakes and Soybean and Phyllanthus niruri Composition Intake

International Journal of Integrated Health Sciences VOL 3, NO 2, September (2015)
Publisher : International Journal of Integrated Health Sciences

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Abstract

Objective: The objective of this study is to examine the relation between nutrition and brain memory. Qualified human resource is the most valuable national asset. Adequate nutrition and regular exercise provide higher achievement to create healthy and smart generations.Methods: The method applied in this study was experimental laboratory. This study was conducted on Juni 2011–Juli 2012 at the Biomedical and Biochemistry laboratory, Faculty of Medicine, Universitas Padjadjaran. The subjects were 8 weeks approximately 200–250 grams, male Wistar rats. The examination involved 56 male Wistar rats divided into 8 groups with different treatments. The groups which received exercise treatment were also given Glycine max and Phyllanthus niruri intakes. Results: NR2B gene expression changes can be described as the following results: (1) without practicing exercise (3,8) and after exercises (4,6); (2) Glycine max intake minus exercise (2,86) and Glycine max intake and exercises (3,17); (3) Phyllanthus niruri intake minus exercises (4,7) and Phyllanthus niruri intake and exercises (4,9); and (4) Glycine max and Phyllanthus niruri composition intake minus exercises (3,14) and Glycine max and Phyllanthus niruri composition intake and exercises (4,83).Conclusions: This study determines that exercises and Phyllanthus niruri intake enhance NR2B gene expressions. Glycine max inhibits the NR2B gene expressions. Glycine max and Phyllanthus niruri composition intake and either with or without practicing exercises enhance NR2B gene expressions. Therefore, practicing exercises and Phyllanthus niruri intake might cause brain cell apoptosis while Glycine max intake inhibits brain cell apoptosis. Keywords: Exercise, gene expressions, Glycin max, NR2B, Phyllanthus niruri DOI: 10.15850/ijihs.v3n2.582

Aktivitas Antijamur Fraksi Air Sarang Semut Myrmecodia Pendens Pada Candida Albicans ATCC 10231

Majalah Kedokteran Bandung Vol 49, No 1 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Penggunaan tanaman herbal untuk pengobatan dan pencegahan penyakit semakin populer sehingga penelitian mengenai senyawa aktif dari tumbuhan yang berkhasiat semakin menjadi perhatian. Umbi sarang semut (Myrmecodia pendens) mengandung senyawa aktif berupa terpenoid, tanin, fenol, flavonoid, dan saponin yang memiliki efek antijamur pada Candida albicans. Penelitian ini bertujuan mengukur konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) fraksi air M. pendens dan menguji perbedaan efek antijamur antara fraksi air M. pendens dan nistatin pada C. albicans ATCC 10231. Penelitian dilaksanakan pada bulan Juni–Juli 2015 di Laboratorium Penelitian Jurusan Kimia Fakultas MIPA Universitas Padjadjaran. Penelitian ini menggunakan metode mikrodilusi dan nilai KHM diukur dengan  enzyme linked immunosorbent assay reader dan nilai KBM diukur dengan pengujian pada media agar. Data dianalisis menggunakan uji-t dengan level signifikan p<0,05 untuk menentukkan perbedaan efek antijamur fraksi air M. pendens dengan nistatin pada C. albicans. Hasil penelitian menunjukkan bahwa nilai KHM ialah 1.250 μg/mL dan nilai KBM ialah 2.500 μg/mL. Simpulan penelitian ini ialah nilai KHM serta KBM fraksi air M. pendens ialah 1.250 dan 2.500 μg/mL dan terdapat perbedaan efek antijamur antara fraksi air dan nistatin terhadap C. albicans. [MKB. 2016;49(1):28–34]Kata kunci: Candida albicans, fraksi air, konsentrasi bunuh minimum, konsentrasi hambat minimum, Myrmecodia pendensAntifungal Activity of Ant Hill Myrmecodia Pendens Water Fraction against Candida Albicans ATCC 10231The use of herbal plant for treatment and prevention of diseases is getting more popular, emphasizing the need for studies on active compounds from plants. Ant hill (Myrmecodia pendens) contains active compounds such as terpenoid, tannin, phenol, flavonoid, and saponin which have antifungal effects on Candida albicans. The objectives of the study were to measure the value of minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of water fraction of M. pendens and antifungal effect of water fractions of M. pendens against C. albicans compared to nystatin. This study used microdilution and the effects were measured with enzyme linked immunosorbent assay reader to determine MIC value, followed by agar media assay to determine  MFC. Data were analyzed using T test with significant level p < 0.05 to determine antifungal effect of water fractions of M. pendens against C. albicans compared to nystatin. The result showed that MIC value was 1.250 μg/ml and MFC value was 2.500 μg/ml. T test showed significant difference of % inhibition cells growth effect between M. pendens water fraction and nystatin (p=0.014 < 0.05). It is concluded that the M. pendens water fraction has an antifungal effect against C. albicans with MIC and MFC values of 1.250 and 2.500 μg/ml.There are differences in antifungal effects between water fraction of M. pendens and nystatin against C. albicans. [MKB. 2016;49(1):28–34]                                        Key words: Candida albicans, minimum fungicidal concentration, minimum inhibitory concentration,  Myrmecodia pendens, water fraction

Metode Cepat Identifikasi Flavonoid dari Daun Ocimum sanctum L. (Selasih)

Majalah Kedokteran Bandung Vol 44, No 1 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Manfaat suatu tumbuhan sebagai obat herbal diperoleh dari kandungan kimianya, misalnya flavonoid. Flavonoid bermanfaat untuk kesehatan tubuh manusia. Oleh karena itu, kandungan flavonoid dapat dijadikan sebagai acuan manfaat suatu tanaman dan dibutuhkan metode identifikasi flavonoid yang cepat. Tujuan penelitian untuk memperoleh metode cepat kandungan flavonoid dalam Ocimum sanctum. Telah dilakukan penelitian ekstraksi, isolasi, dan identifikasi flavonoid daun Ocimum sanctum di laboratorium Kimia Medik Fakultas Kedokteran Universitas Padjadjaran pada tahun 2005. Flavonoid diekstraksi sinambung dengan etanol 95%, diisolasi dengan kromatografi kertas/lapis tipis selulose dan asam asetat 2%, kemudian dilanjutkan dengan pengembang: n-butanol–asam asetat–air, kloroform–asam asetat–air, forestall, asam asetat 5%, 15%, 35%, dan 50%. Flavonoid diidentifikasi dengan pereaksi geser dan sinar ultraviolet. Hasil isolasi dengan asam asetat 2% dan 35% berturut-turut memberikan dua bercak flavonoid, sebagai flavon, FOAc-1 mempunyai Rf=0,69 dan FOAc-2 mempunyai Rf=0,57. Pengembang lainnya memberikan satu bercak flavonoid. Simpulan, isolasi dengan asam asetat 2% dan 35% berturut-turut serta identifikasi dengan pereaksi geser dan sinar ultraviolet merupakan metode yang cepat untuk identifikasi kandungan flavonoid dalam Ocimum sanctum. [MKB. 2012;44(1):32–7]. Kata kunci: Flavonoid, identifikasi flavonoid, Ocimum sanctumRapid Identification Method of Flavonoid from Ocimum sanctum L. (Selasih) Leaves A plant’s effectiveness as a herbal drug comes from its chemical content such as flavonoids. Flavonoids are useful for human body health. Therefore flavonoids content can be used as a marker from the usefulness of a plant, and rapid identification method of flavonoid is needed. The objective of the research was to get a rapid method of flavonoid content identification from Ocimum sanctum. The extraction, isolation and identification of flavonoids from Ocimum sanctum leaves has been done at Medical Chemistry laboratory Faculty of Medicine Universitas Padjadjaran in 2005. Flavonoids were extracted continually by 95% ethanol, were isolated with paper chromatography/cellulose TLC and 2% acetic acid, then continued with elucidation reagents: n-butanol–acetic acid–water, chloroform–acetic acid–water, forestall, 5%, 15%, 35% and 50% acetic acids. Flavonoids were identified with diagnostic reagents and ultraviolet light. Isolation with 2% and 35% acetic acids respectively gave two spots of flavonoids as flavon, FOAc-1 had Rf=0.69 and FOAc-2 had Rf=0.57. The other elucidation reagents gave one spot of flavonoid. In conclusion, isolation with 2% and 35% acetic acids respectively and identification with diagnostic reagent and ultraviolet light is a rapid method for identification of flavonoids content in Ocimum sanctum. [MKB. 2012;44(1):32–7].Key words: Flavonoid, identification of flavonoid, Ocimum sanctum DOI: http://dx.doi.org/10.15395/mkb.v44n1.73