Nyoman Sadra Dharmawan
Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Articles
29
Documents
Crude Antigen Cystisercus Taenia Saginata Isolat Bali untuk Deteksi Sistiserkosis pada Sapi

Jurnal Ilmu dan Kesehatan Hewan (Veterinary Science and Medicine Journal) Vol 2, No 1 (2014)
Publisher : Jurnal Ilmu dan Kesehatan Hewan (Veterinary Science and Medicine Journal)

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Abstract

The purpose of this study was to evaluate Taenia saginata cystisercus antigen for the detection of bovine cysticercosis. Taenia saginata cysticercus antigen was derived from local isolates, obtained from the experimental infection of Taenia saginata tape worms from Bali. The research was done by ELISA (Enzyme Linked Immunosorbent Assay) optimized by determining the optimal concentration of antigen, the optimal dilutions of serum and the optimal dilutions of conjugate. The results showed that Taenia saginata cysticercus crude antigen (Bali isolate) are antigenic and can be used to detect cattle cysticercosis. Optimal concentration of antigen: 2 ug/ml, optimal dilutions of serum: 1:80 and optimal dilution of conjugate: 1:4000.

Seroprevalensi Neospora caninum pada sapi bali yang dipotong di Rumah Potong Hewan (RPH) Denpasar

Jurnal Ilmu dan Kesehatan Hewan (Veterinary Science and Medicine Journal) Vol 2, No 2 (2014)
Publisher : Jurnal Ilmu dan Kesehatan Hewan (Veterinary Science and Medicine Journal)

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Abstract

Recent years the infection of Neospora caninum parasite has been a significant reproductive disease on cattle. The purpose of this study was to determine the seroprevalence of Neospora caninum in Bali cattle that are slaughtered in Denpasar’s slaughter house.A total of 184 serums was collected and tested using enzyme linked immunosorbent assay (ELISA). The results showed that there were 2 serum samples (1.08%) contain Neospora caninum antibodies. While the remaining samples, 182 samples (98.92%), were negative. Though the neosporosis seroprevalence rate in Bali is very low, a sustainable surveylance must be carried out to mitigate the broadness of the disease distribution.