Nani Dharmasetiawani
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Published : 6 Documents
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Elastase-1 concentration in feces of term and preterm infants aged 0 – 4 months Dharmasetiawani, Nani; Firmansyah, Agus
Medical Journal of Indonesia Vol 12, No 2 (2003): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.152 KB) | DOI: 10.13181/mji.v12i2.95

Abstract

Fecal elastase-1 concentration reflects exocrine pancreatic function. There have been some reports from Europe, but so far there has not been a report of fecal elastase-1 concentration in Indonesia, especially concerning infants. The aim of this study is to know the concentration of elastase-1 in feces of infants aged 1-120 days as a preliminary report of the study of the ontogeny of pancreatic elastase-1 in term and preterm infants. Fecal elastase-1 were measured from feces of 28 healthy preterm and 34 healthy term infants up to 120 days (4 month) of age. Elastase-1 concentration in infants less than 14 days of age fluctuated below 200 μg/gram feces. At the first day of life 80% preterm and 60% term infants had elastase-1 concentration less than 200 μg/gram feces, and by the age of 7 days 50% preterm and 33% term infants had elastase-1 concentration less than 200 μg/gram feces. After 14 days of age its concentration was more than 200 μg/gram feces, regardless of gestational age. This preliminary study corroborates supported the previous studies that the level of fecal elastase-1 reached normal level after 14 days. Future longitudinal study is needed to know elastase-1 concentration in infants less than 14 days. (Med J Indones 2003; 12: 69-72) Keywords: Fecal elastase-1, Infants, Enzyme-link immunosorbent assay
Quantitative analysis of lactose and lactulose in urine by high performance liquid chromatography for determination of intestinal lactase activity Dharmasetiawani, Nani; Suryadi, Herman; Firmansyah, Agus
Medical Journal of Indonesia Vol 12, No 1 (2003): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.365 KB) | DOI: 10.13181/mji.v12i1.84

Abstract

Determination of intestinal lactase activity is directly done by measuring its activity in intestinal epithelium. This is an invasive method and ethically can not be done in healthy infants. Indirectly, determination of lactase activity, stated as excretion and ingestion ratio of lactose and lactulose, needs 30 hours hospitalized infants. The aim of this study was to look for a method for determination of lactase activity which is not invasive and not necessary hospitalized. Using this method  lactose and lactulose were given as a single oral load after 2 hours fasting. Urine were collected for 5 hours starting from consuming sugar solution and then lactose and lactulose concentration in the urine were measured by High Performance Liquid Chromatography. The results showed single oral load of lactose and lactulose can be used for determination of lactase activity in infant and the infants were observed only for 7 hours. (Med J Indones 2003; 12: 8-12) Keywords: Lactase activity, Lactulose, Lactose, High Performance Liquid Chromatography
Prevalens dan Faktor Risiko Terjadinya Hipozincemia Bayi Berat Lahir Rendah pada Usia Koreksi Mendekati Cukup Bulan atau Cukup Bulan Kaban, Risma Kerina; Dharmasetiawani, Nani; Siswanto, Johanes Edy
Sari Pediatri Vol 13, No 3 (2011)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp13.3.2011.207-14

Abstract

Latar belakang. Deposit Seng (Zn) berperan terhadap fungsi metabolik tubuh. Bayi kurang bulan mempunyai cadangan Zn yang rendah pada masa fetus, kebutuhan Zn yang tinggi setelah lahir, kapasitas untuk mengabsorbsi dan retensi zat makanan terbatas. Gambaran klinis dari defisiensi Zn yang berat yaitu dermatitis, iritabel, kandidiasis oral, diare, mineralisasi tulang yang buruk, gangguan fungsi motorik dan kognitif, meningkatnya risiko terkena infeksi, dan retardasi pertumbuhan.Tujuan. Untuk menentukan prevalens dan faktor risiko hipozincemia pada bayi berat lahir rendah.Metode.Penelitian prospektif, desain penelitian potong lintang. Data dikumpulkan dari 3 rumah sakit di Jakarta. Informasi faktor risiko dicatat dan kadar Zn diperiksa pada bayi dengan berat lahir <2000 g dan usia gestasi <34 minggu, pada usia koreksi mendekati cukup bulan atau cukup bulan. Hasil dianalisis dengan (T-test, dan Mann-Whitney) (regresi logistik). Defisiensi Zn didiagnosis apabila kadar Zn <55mg/dl (8,4 μmol/L).Hasil. Dari 63 bayi yang diteliti terdapat 18 bayi yang hipozincemia sehingga didapatkan prevalens hipozincemia 28%. Dari 18 bayi hipozincemia, 67% disertai dengan gejala yang paling banyak dijumpai adalah gangguan pertumbuhan. Peningkatan usia gestasi, peningkatan kadar kalsium dan pemberian suplemen besi berhubungan dengan penurunan risiko hipozincemia (OR 0,622; 95% CI: 0,42-0,92), (OR 0,376; 95% CI: 0,16 – 0,88) dan (OR 0,062; 95 % CI: 0,008-0,46). Sedangkan jenis kelamin laki laki berhubungan dengan peningkatan risiko hipozincemia(OR 4,764; 95% CI: 0,06-21,40).Kesimpulan.Prevalens hipozincemia pada bayi usia gestasi <34 minggu dan berat lahir <2000 gram, yang diperiksa pada usia koreksi >35 minggu, didapatkan 28% dengan gejala. Gangguan pertumbuhan merupakan gejala yang paling banyak dijumpai. Faktor risiko hipozincemia ditemukan pada bayi laki-laki, usia gestasi yang lebih rendah, penurunan kadar kalsium dan tanpa pemberian suplementasi besi.
Altered expressions of endothelial junction protein of placental capillaries in premature infants with intraventricular hemorrhage Ekawati, Maria; Mujihartini, Ninik; Jusuf, Ahmad A.; Dharmasetiawani, Nani; Jusman, Sri W.A.; Sadikin, Mohamad
Medical Journal of Indonesia Vol 25, No 3 (2016): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (691.713 KB) | DOI: 10.13181/mji.v25i3.1287

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Background: Placental hypoxia may lead to oxidative stress, which inflicts damage to capillary protein junction. The aim of this study was to evaluate altered expression of endothelial junction protein of capillaries in hypoxia condition and to observe its correlation with the incidence of  intraventricular hemorrhage in premature infants.Methods: A cross-sectional study was conducted by using placental tissues of premature infants as amodel of capillary integrity (29 hypoxic and 29 non-hypoxic). Hypoxia inducible factor (HIF)-1α was measured to define placental tissue response to hypoxia; malondialdehyde (MDA) and glutathione (GSH) served as markers of oxidative stress. The expressions of junctional proteins, N-cadherin and occludin were analyzed by immunohistochemistry. Intraventricular hemorrhage (IVH) was detected by cranial ultrasound at the third day. Unpaired t test, Mann-Whitney, and Chi-square tests were used to analyze the data.Results: The HIF-1α and MDA levels were slightly, but not significantly, higher in hypoxia group {13.64±8.70 pg/mg protein and 10.31 pmol/mg tissue (ranged 1.92–93.61), respectively}  compared to non- hypoxia group {10.65±5.35 pg/mg protein and 9.77 pmol/mg tissue (ranged 2.42–93.31)}. GSH levels were not different in both groups (38.14 (ranged 9.44–118.91) and  38.47(ranged 16.49–126.76) ng/mg protein, respectively. mRNA expression of N-cadherin (0.13) and occludin (0.096) were significantly lower in hypoxia comparedto non-hypoxia group (p=0,001), while protein expression of  N-cadherin (3.4; 75.9; 6.9; 13.8%) and occludin  (20.7; 3.4; 69.0; 3.4; 6.9%)  in hypoxia group was not associated with IVH (p=0.783 and p=0.743).Conclusion: Hypoxia altered expression of endothelial junction protein in placental capillaries, but no association with intraventricular hemorrhage was observed.
The effect of Bifidobacterium animalis lactis HNO19 supplementation among pregnant and lactating women on interleukin-8 level in breast milk and infant’s gut mucosal integrity Dewanto, Naomi E.F.; Firmansyah, Agus; Sungkar, Ali; Dharmasetiawani, Nani; Sastroasmoro, Sudigdo; Kresno, Siti B.; Suradi, Rulina; Bardosono, Saptawati; Prasetyo, Dwi
Medical Journal of Indonesia Vol 26, No 3 (2017): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.297 KB) | DOI: 10.13181/mji.v26i3.1481

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Background: Newborn’s gut mucosal is not fully developed, therefore infants are prone to diarrhea. Probiotic supplementation is known to induce the gut mucosal maturity. This study aimed to identify whether probiotics supplementation among pregnant women since the third trimester would increase the infant’s gut mucosal integrity.Methods: A double-blind, randomized clinical trial was conducted to understand the potential effect of probiotic supplementation on the level of probiotics and IL-8 in breastmilk, urine IFABP, faecal α-1-antytripsin (AAT) and calprotectin in infant’s at birth (V0) and three-months old (V3). A single strain of Bifidobacterium lactis animalis HNO19 (known as DR10) was used since it was not the resident bacteria. The study was held at Budi Kemuliaan Hospital and its satellite clinics from December 2014 to December 2015.Results: About 14% (5/35) and 20% (7/35) of the subjects had DR10 in the breastmilk’s colostrum and at the age of 3-months. The median values of IL-8 in the probiotic group vs the placebo group at V0 and V3 were 2810,1 pg/mL vs 1516.4 pg/mL (p=0.327) and 173.2 pg/mL vs 132.7 pg/mL (p=0.211) respectively. IFABP level 211.7 ng/mL vs 842.5 ng/mL (p=0.243) and 25.3 ng/mL vs 25.1 ng/mL (p=0.466); AAT 136.2 mg/dL vs 148.1 mg/dL (p=0.466) and 24 mg/mL vs 29.72 mg/mL (p=0.545); Calprotectin 746.8 ng/mL vs 4645.2 ng/mL (p=0.233) and 378.6 ng/mL vs 391.3 ng/mL (p=0.888).Conclusion: Probiotic DR10 given to pregnant women since the 3rd trimester can be found in colostrum and 3-months breastmilk. However, it did not affect the level of other probiotics or IL-8 and the gut mucosal integrity.
Tumor necrosis factor-alpha and interleukin-6 in early-onset neonatal sepsis Rukmono, Prambudi; Dharmasetiawani, Nani; Warsono, Warsono; Wirasti, Yan; Darwin, Eryati
Paediatrica Indonesiana Vol 56 No 1 (2016): January 2016
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.152 KB) | DOI: 10.14238/pi56.1.2016.15-8

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Background Neonatal sepsis remains a major cause of mortality and morbidity in newborns. Early-onset neonatal sepsis occurs in infants under the age of 72 hours, while late-onset neonatal sepsis occurs in infants over the age of 72 hours and may be due to nosocomial infection. Diagnosing neonatal sepsis is a challenge, as its clinical symptoms are not clear. Corroborating tests include routine blood, C-reactive protein (CRP), serology, tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) examinations.Objective To compare the TNF-α and IL-6 levels in patients with proven and unproven early-onset neonatal sepsis (EONS)Methods This case-control study was done in the Perinatology Unit, Abdul Moeloek Hospital, Lampung. Subjects were under the age of 72 hours with risk factors and clinical symptoms of sepsis. They underwent routine blood tests and blood cultures. Infants with positive cultures were considered to have proven sepsis (26 subjects) and infants with negative blood cultures were considered to have unproven sepsis (26 subjects). All subjects underwent serological examinations of TNF-α and IL-6.Results There were no differences in the basic characteristics of subjects between the two groups. Levels of TNF-α in the sepsis group were significantly higher than in the unproven group [(28.30 vs. 10.96 pg/mL, respectively (P=0.001)]. Furthermore, Il-6 was significantly higher in the proven sepsis group than in the unproven sepsis group [(28.3 vs. 9.69 pg/mL, respectively) (P=0.006)].Conclusion Levels of TNF-alpha and IL-6 are significantly higher in infants with proven than unproven early-onset neonatal sepsis.