Sri Sinto Dewi
Program Pascasarjana, Program Studi Magister Ilmu Biomedik Universitas Diponegoro, Jl. Hayam Wuruk No. 5 Lt. III Semarang - 50241

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Efek Ekstrak Etanol Morinda Citrifolia L (Mengkudu) Terhadap Kadar Gula Darah, Jumlah Neutrofil, dan Fibronektin Glomerulus Tikus Diabetes Mellitus Kustarini, Indranilla; Dewi, Sri Sinto; M, Ika Pawitra
MEDIA MEDIKA INDONESIANA 2012:MMI VOLUME 46 ISSUE 3 YEAR 2012
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

Ethanol extract of Morinda citrifolia L (mengkudu), blood glucose, neutrophyl count, and glomerulus fibronectin in diabetes mellitus ratBackground: Diabetes mellitus is a metabolic disease which caused by the present of insulin abnormality. Diabetes is shown by hyperglycemia to release free radical, superoxide, that induce oxidative stress. This stress will increase mesangial matrix production in glomerulus such as fibronectin. Ethanol extract Morinda citrifolia contain fenolat and flavonoid which have antioxidant system toblock the oxidative stress. This research purpose was to analyze the effect of ethanol extract Morinda citrifolia to blood glucose,  neutrophyl count, glomerulus fibronectin of diabetes mellitus rats.Method: Using the post test only control group design, diabetic Sprague Dawley rats induced streptozotocin dose 40mg/kgBW, were divided into 4 groups of grading doses (10, 20, 40, 80mg/dl) of ethanol extract Morinda group and 1 control group. Each group used 6 male rat. Blood glucose, neutrophyl count and glomerular fibronectin were examined. Data analyze with Kruskall Wallis and ANOVA using SPSS ver 15.0Result: The mean of blood glucose before and after treating with extract ethanol Morinda were 553.04mg/dl and 408.81mg/dl respectively. Different score of neutrophyl count was p=0.0574 and fibronectin expression was p=0.000. Ethanol extract Morinda citrifolia can reduce blood glucose, and the fibronectin expression in glomerulus of diabetic rats significantly, but not for neutrophyl count. The lowest fibronectin expression occurred on dose 20 mg/dl.Conclusion: Extract ethanol Morinda citrifolia L (Mengkudu) improve diabetic nephtopathy through reducing blood glucose, netrophyl count, and fibronectin.Keyword: Morinda citrifolia, blood glucose, neutrophyl count, glomerular fibronektin ABSTRAKLatar belakang: Diabetes mellitus merupakan penyakit metabolik yang disebabkan karena adanya kelainan sekresi atau kerja insulin. Diabetes mellitus ditandai dengan adanya hiperglikemi, dimana pada kondisi ini akan melepaskan radikal bebas, superoksid, yang akan menyebabkan stres oksidatif, dengan akibat kerusakan sel baik sel neutrofil, sel mesangial ginjal yang akan mengeluarkan matrik ekstra sel berupa fibronektin. Ekstrak etanol Morinda citrifolia L memiliki kandungan fenolat dan flavonoid, dapat membantu sistem antioksidan dalam tubuh yang dapat mengatasi stres oksidatif. Penelitian ini bertujuan untuk menganalisis efek ekstrak etanol Morinda citrifolia L terhadap kadar gula darah, jumlah neutrofil, dan ekspresi fibronektin glomerulus tikus diabetes mellitus.Metode: Penelitian ini menggunakan pendekatan post test only control group design pada tikus Sprague Dawley yang diinduksi Streptozotozin (dosis 40mg/kgBB) sehingga  menjadi DM, kemudian dikelompokkan menjadi 4 kelompok perlakuan ekstrak etanol Morinda citrifolia L (Mengkudu) dengan dosis 10, 20, 40, 80mg/dl dan 1 kelompok kontrol DM. Data dianalisis menggunakan uji Kruskal Wallis dan ANOVA dengan SPSS ver 15.0.Hasil: Rerata kadar gula darah sebelum 553,04 mg/dl dan sesudah diberi Morinda 408,81 mg/dl. Hasil menunjukkan perubahan jumlah neutrofil diperoleh nilai p=0,0574, dan ekspresi fibronektin glomerulus diperoleh nilai p=0,000. Ekstrak etanol Morinda citrifolia L menurunkan kadar gula darah dan ekspresi fibronektin secara bermakna, namun tidak bermakna pada jumlah neutrofil. Dosis 20 mg/dl merupakan dosis yang paling baik menurunkan ekspresi fibronektin glomerulus.Simpulan: Ekstrak etanol Morinda citrifolia L (Mengkudu) memperbaiki fungsi ginjal diabetes nefropati melalui penurunan gula darah, jumlah neutrofil dan fibronektin.
Bacterial Plasmids Profile from Escherichia coli Resistant to Metronidazole and Nalidixic Acid Iswara, Arya; Dewi, Sri Sinto
el–Hayah Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Jurusan Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i1.4079

Abstract

bacteria that cause an illness. Antibiotic treatments to a patient have a purpose to eliminate the pathogen bacteria. Bacteria resistance to antibiotic was influenced by the intensity of antibiotic treatment in a region, the uncontrolled antibiotics treatments would increase the antibiotic resistance of bacteria. Plasmids was an extrachromosomal DNA that encodes a functional protein that would eliminate the antibiotic activity. Plasmid is the determinant of bacteria sensitivity to antibiotics. In this case it would be important to find out the bacterial plasmid profile on the E.coli resistant to metronidazole and nalidixic acid antibiotics. This research was using four different sample from faces of diarrhea, ice block, waters from well, and ketchup to cultivate the E. coli. lasmid isolation method was carried out by lyses alkali method. Plasmid profile of the E. coli that resistant to metronidazole and nalidixic acid antibiotics and analyzed using electrophoresis on 1% agarose. E. coli plasmid DNA profile was observed as a fluorescent DNA band in ultraviolet rays. In result, isolated plasmids from bacteria that resistant to antibiotics metronidazole and nalidixic acid having similar size approximately 500 bp, different from bacteria that sensitive to antibiotics metronidazole and nalidixic acid has a smaller size in region of 100 bp.
Pengecatan Imunohistokimia HER2 Menggunakan Susu Skim dan Normal Serum Iswara, Arya; Dewi, Sri Sinto; Masruro, Yulfa Ariza
Jurnal Biomedika Vol 10 No 1 (2017): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (587.018 KB) | DOI: 10.31001/biomedika.v10i1.226

Abstract

HER2 (Human Epidermal Growth Factor Receptor 2) merupakan suatu reseptor pada permukaan sel yang berpengaruh pada proliferasi jaringan, mutasinya dapat menjadi onkogen. Over ekspresi dari HER2 pada kasus kanker dapat dilihat dengan teknik imunohistokimia (IHC). Protein blocking merupakan salah satu langkah dalam pengecatan IHC yang berfungsi menghalangi ikatan non spesifik pada jaringan dengan menggunakan normal serum dan protein solution (susu skim). Tujuan penelitian mengetahui gambaran hasil pengecatan IHC menggunakan normal serum dan susu skim. Penelitian secara eksperimental dengan pendekatan cross sectional. Sampel penelitian jaringan kanker payudara HER2 positif dengan stadium +2 dari satu organ dan pasien yang sama. Pengecatan IHC menggunakan teknik Strep (Avidin) Biotin Complex. Pengecatan menggunakan normal serum didapatkan hasil +2, menggunakan susu skim 1% didapatkan hasil +3, sedangkan menggunakan susu skim 2% dan 3% didapatkan hasil +2. Terdapat perbedaan yang signifikan antara normal serum dengan susu skim 1%. Tidak terdapat perbedaan yang signifikan antara normal serum dengan susu skim 2% dan susu skim 3%. Simpulan adalah normal serum dapat diganti dengan susu skim 2%.