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PENINGKATAN PERFORMA REPRODUKSI IKAN PATIN SIAM (Pangasianodon hypophthalmus) PADA MUSIM KEMARAU MELALUI INDUKSI HORMONAL [Reproduction Improvement on Female Striped Catfish Pangasianodon hypophthalmus at Dry Season By Hormonal Induction] Tahapari, Evi; Dewi, Raden Roro Sri Pudji Sinarni
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.533

Striped catfish (Pangasianodon hypophthalmus) spawning success is strongly influenced by environment.Striped catfish spawning generally occurs during the rainy season while in the dry season is difficult to find mature female. This research was conducted to improve reproduction performance of female striped catfish during dry season through combination injection of hormone pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG).The treatments were A : control (no hormone injections), B : injection of PMSG 10 IU/kg + HCG 10 IU/kg and C : injection of PMSG 20 IU/kg + HCG 10 IU/kg.Injections were done at interval of two weeks as many as six times. The research was conducted during dry season in June-October 2012 with rainfall 0.20- 14.40 mm/years. The results showed combination injection of PMSG + HCG could improve female reproduction performance. The best treatment was shown on C treatment with values of OSI (ovi somatic index), fecundity, fertilization rate, hatching rate and larva production was 12.18 %, 523 000 eggs/female, 79.65 %, 69.93 % and 290 000 larvae/female respectively. As for control treatment (A) the value of OSI, fecundity, fertilization rate, hatching rate and larval production, was 6.45 %, 197 000 eggs/female, 48.55 %, 35.59 % and 34 000 larvae/female, respectively

DISTRIBUTION AND EXPRESSION OF STRIPED CATFISH (Pangasionodon hypophtalmus) GROWTH HORMONE GENE (PhGH) IN THE ORGAN OF AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC FOUNDER Marnis, Huria; Dewi, Raden Roro Sri Pudji Sinarni; Imron, Imron; Iswanto, Bambang
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Faster growing African catfish can be produced by transgenesis. This study wasÂ conducted to investigate the distribution and expression of growth hormone geneÂ (PhGH) in various organs of the transgenic African catfish (Clarias gariepinus) founderÂ (F0). Transgene was detected using the PCR method in various organs, namely pituitary,Â brain, liver, heart, spleen, kidney, intestine, stomach, muscle, caudal fin, gill and eye.Â Transgene expression levels were analyzed using the method of reverseÂ transcriptase-polymerase chain reaction (RT-PCR), ï¢-actin gene used as internalÂ controls. The results showed that the PhGH was detected and expressed in all organsÂ of the transgenic African catfish founder. The high level of PhGH expression wasÂ found in the liver, pituitary, intestine and brain; smaller amounts were detectable inÂ muscle, spleen, kidneys, heart, and stomach, caudal fin, gill and eyes, range fromÂ 0.02-0.75 PhGH/ï¢-actin mRNA. The expression levels of PhGH had positive correlationÂ with tissue and body size (P<0.05).

IMPROVED PRODUCTION OF TIGER SHRIMP (Penaeus monodon) THROUGH PROBIOTICS APPLICATION Insan, Irsyaphiani; Kontara, Endhay Kusnendar; Dewi, Raden Roro Sri Pudji Sinarni
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.10.2.2015.131-136

The study was carried out in Brebes District, the North coast of Java. Tiger shrimp farming in Indonesia,Â particularly in this area faced some problems which caused by improper pond preparation, disease, andÂ low seed quality. Probiotic was applied in pond to solve this problem. The aim of this study was to evaluateÂ the production of tiger shrimp in ponds with probiotic applications. Six experimental ponds (each measuringÂ 0.5 ha) were selected of which three were probiotic ponds and three were controlled. Tiger shrimp postlarvaeÂ (PL-30) were stocked at density of four shrimps/m2. Tiger shrimps were reared for three months.Â Shrimps were fed by commercial pellet. In the first month, shrimp were fed about 7%-5% of the totalÂ biomass; in the second months, 3.5%-3% of the total biomass; and in the third month, 2.5%-2% of the totalÂ biomass. The treatments in this study were the application of probiotics with concentration of 3 mg/L thatÂ were given every five days and control (without probiotics). The results showed the rearing period was 92Â Â± 6 days in probiotic ponds and 76 Â± 16 days in controlled pond. The shrimp in controlled pond shouldÂ be harvest earlier caused by the high mortality. The average final weight was 16.2 Â± 0.7 g in probioticÂ pond and 15.6 Â± 1.9 g in controlled pond. The survival rate was 64.13 Â± 12.63% in probiotic pond andÂ 44.17 Â± 14.15% in controlled pond. Production was 208 Â± 46 kg/pond/cycle in probiotic pond and 123 Â±Â 6 kg/pond/cycle in controlled pond. The result showed that probiotic plays an important role in maintainingÂ water quality parameters and health management as well as increases the survival of shrimp.

OPTIMAL ELECTROPORATION CONDITION FOR SPERM MEDIATED GENE TRANSFER IN STRIPPED CATFISH (Pangasionodon hypophthalmus) Dewi, Raden Roro Sri Pudji Sinarni; Alimuddin, Alimuddin; Sudrajat, Agus Oman; Sumantadinata, Komar; Sularto, Sularto
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

The success of transgenic fish production has been achieved through eggsÂ fertilization using electroporated sperms carrying exogenous DNA. This study wasÂ conducted in order to obtain the optimal electroporation condition for stripped catfishÂ sperm. A plasmid containing green fluorescent protein (GFP) gene driven by carp Î²-actin promoter was transferred into sperm using electrophoresis method towardsÂ transgenic stripped catfish (Pangasionodon hypophthalmus) production.Â Electroporation was carried out using square wave shock with pulse length of 30 msÂ and pulse interval of 0.1 sec. Treatments are combination between voltage (50 V, 75 V,Â and 100 V) and pulse number (1 and 3). Exogenous DNA concentration used was 10Â Î¼g/mL of Tris-EDTA. Results showed that increasing the voltage from 50 to 100Â decreased sperm motility, while pulse number did not affect sperm motility. VoltageÂ of 50 gave the best motility of sperm, although sperm viability relatively similarÂ between treatments and control except at 100 V with 3 pulses number. Further,Â electroporation-treated sperms were able to fertilize eggs. Higher hatching rate ofÂ eggs was obtained in electroporation treatment at 50 V with pulse number of 1 and 3.Â The persistence of transferred GFP was detected in electroporated and incubatedÂ sperms (control). However, GFP was only detected in larvae from eggs that wereÂ fertilized by electroporated sperm. Thus, electroporation could be applied to produceÂ transgenic stripped catfish.Â

NUTRITIVE COMPOSITION OF RED TILAPIA REARED IN FRESHWATER AND BRACKISHWATER Dewi, Raden Roro Sri Pudji Sinarni; Setyawan, Priadi; Tahapari, Evi; Robisalmi, Adam; Listiyowati, Nunuk
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

The aim of this research was to investigate the nutritive composition (especiallyÂ fatty acids) in red tilapia that was reared in freshwater and brackishwater. The fattyÂ acid contents were determined by gas chromatography. The fatty acids profile wereÂ ï·-3 (linolenic acid, eicosapentaenoic acid/EPA, docosahexaenoic acid/DHA), ï·-6Â (linoleic acid, arachidonic acid/AA), and ï·-9 (oleic acid). Red tilapia samples wereÂ obtained from Research Institute for Fish Breeding, Sukamandi, West Java (freshwaterÂ ponds) and Congot, Yogyakarta (brackishwater ponds; salinity 20 ppt). In this research,Â red tilapia reared in different ecosystems showed different fatty acid profiles. RedÂ tilapia inhabiting brackishwater ecosystem has EPA (0.26Â±0.05%), DHA (3.42Â±0.26%),Â and linoleic acid (17.20Â±0.56%) content higher than freshwater ecosystem (EPA = 0%;Â DHA = 0.67Â±0.44%; linoleic acid = 9.08Â±4.76%), except for linolenic acid (0.30Â±0.15%Â vs 0.25Â±0.10%), arachidonic acid (0.77Â±0.39% vs 0.93Â±0.13%) and oleic acidÂ (38.67Â±2.58% vs 37.44Â±0.74%). The ratio of ï·-6/ï·-3 in red tilapia inhabiting freshwaterÂ ecosystem was about 11/1. The culture tilapia in brackishwater ecosystem decreaseÂ ï·-6/ï·-3 ratio (4.5:1). So that for human health, it will be better to consume brackishwaterÂ red tilapia than freshwater red tilapia.

DETECTION, TRANSMISSION, AND EXPRESSION cDNA GROWTH HORMONE GENE (PhGH) OF STRIPPED CATFISH IN F-1 TRANSGENIC AFRICAN CATFISH Marnis, Huria; Iswanto, Bambang; Suprapto, Rommy; Imron, Imron; Dewi, Raden Roro Sri Pudji Sinarni
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

In previous study, the fast growth transgenic founder of African catfish was producedÂ harboring a growth hormone (GH) gene construct containing a stripped catfish growthÂ hormone (PhGH) cDNA. This study was conducted to investigate transgene (PhGH)Â transmission and expression in F-1 transgenic African catfish. The transgenic foundersÂ (female) were crossed with non-transgenic (male) to produce heterozygous F-1Â progeny. PhGH gene was detected in the embryo, larvae, and seed of the transgenicÂ F-1 using PCR method. Expression levels of transgene in embryo and larvae wereÂ analyzed using real-time quantitative PCR (qPCR) method. The transgene was detectedÂ in embryo, larvae and seed of F-1 transgenic African catfish. Founder could transmitÂ PhGH gene to transgenic F-1 lines in ranged 36% to 48%. Expression level of PhghÂ gene in embryo was higher than that of the larvae; whereas in the embryo was 1.5 xÂ 105 - 5.2 x 105 copies or 0.49-9.82 fold, while in the larvae was 1.1 x 105 - 2.5 x 105copies or 0.19-5.80 fold.Â

PEMANFAATAN PROBIOTIK KOMERSIAL PADA PEMBESARAN IKAN LELE (Clarias gariepinus) Dewi, Raden Roro Sri Pudji Sinarni; Tahapari, Evi
Jurnal Riset Akuakultur Vol 12, No 3 (2017): (September 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Ikan lele Afrika (Clarias gariepinus) merupakan spesies asli Afrika yang telah diintroduksikan dan dibudidayakan secara komersial di Indonesia. Upaya peningkatan efisiensi produksi ikan lele terus ditingkatkan guna meningkatkan keuntungan. Salah satu upaya untuk meningkatkan efektivitas budidaya ikan lele adalah melalui penggunaan probiotik. Penelitian ini bertujuan untuk mengevaluasi pemanfaatan probiotik pada pembesaran ikan lele. Perlakuan yang diberikan berupa pemberian pakan hasil fermentasi probiotik dan pakan tanpa fermentasi (kontrol) dengan tiga ulangan. Pengujian dilakukan pada kolam terpal berukuran 3 m3 dengan padat tebar yang digunakan yaitu 500 ekor/wadah dan dipelihara selama 35 hari. Berdasarkan hasil pengujian, pemberian probiotik pada pakan dengan cara fermentasi mampu meningkatkan bobot dan biomassa panen secara signifikan (P<0,1). Bobot akhir ikan lele yang diberi pakan hasil fermentasi probiotik mencapai 76,9 Â± 0,2 g; sedangkan kontrol 74,2 Â± 0,2 g. Biomassa akhir ikan lele yang diberi pakan hasil fermentasi probiotik mencapai 37,91 Â± 0,29 kg; sedangkan kontrol 34,65 Â± 1,70 kg. Pemberian pakan yang difermentasi probiotik mampu meningkatkan retensi protein sebesar 1,02%; retensi karbohidrat sebesar 10,26%; dan retensi lemak sebesar 7,22%. Selain itu, penggunaan probiotik mampu menekan biaya produksi sebesar Rp 561,00/kg dan meningkatkan keuntungan sebesar 5%.African catfish (Clarias gariepinus) is a native African species that has been introduced and cultivated commercially in Indonesia. Efforts to increase the efficiency of catfish production were conducted in order to increase profit. One effort to increase the effectiveness of catfish farming is through the use of probiotics. This study was aimed to evaluate the use of probiotics in catfish farming. The treatments were fermented feed by probiotic and non fermented feed (control) and repeated three times. The experiment was conducted on a 3 m3 tarpaulin pond, with a density 500 fishes/pond, and reared for 35 days. Based on the results, the fermented feed by probiotic could increase the weight and biomass of harvested fish significantly. The weight of catfish fed with fermented feed reached 76.9 Â± 0.2 g while the control was 74.2 Â± 0.2 g. The final biomass of catfish fed with fermented feed reached 37.91 Â± 0.29 kg while the control was 34.65 Â± 1.70 kg. Feeding fermented feed by probiotic Gut Bio Aero increased the protein retention by 1.02%, carbohydrate retention by 10.26%, and lipid retention by 7.22%. The application of probiotic could reduce production cost by 561 IDR/kg and increased profit by 5%.

THE ABILITY OF FAST-GROWING TRANSGENIC AFRICAN CATFISH (Clarias gariepinus) ON PREDATOR AVOIDANCE Marnis, Huria; Iswanto, Bambang; imron, imron; Febrida, selny; Dewi, Raden Roro Sri Pudji Sinarni
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Research Institute for Fish Breeding has produced transgenic African catfish (Clarias gariepinus) containing stripped catfish growth hormone gene (PccBA-PhGH) with growth 19.86% faster than that of non-transgenic fish. This fish has high potential to be released and utilized for fish farming sector to increase national production. However, there is not yet information about environmental risk of this fish. One of the major fitness traits determining potential environmental risk is predator avoidance. This study aimed to determine the predator avoidance ability of transgenic African catfish in an experimental laboratory condition. In this study, thirty five individuals each of transgenic and non-transgenic with body weight of about 0.1 Â± 0.019 g were communally stocked in 60 cm x 40 cm x 40 cm aquarium with limited feeding frequency (ad libitum twice a day). One day after the fish were stocked, the predators were added to each aquarium. The non-transgenic and transgenic with body weight of 1.0 Â± 0.024 g were stocked as predators as many as five individual in each aquarium. After approximately two weeks of predation, all remaining fish were collected for transgenic verification by PCR method. Genomic DNA was isolated from fin tissue of individually survivors. The results of this study showed that the transgenic fish had worse predator avoidance and lower cannibal than non-transgenic (P<0.05). There was no significant difference in growth performance between transgenic and non-transgenic African catfish (P>0.05) in limited food. The transgenic fish may have lower fitness than non-transgenic.

THE IMPACTS OF FASTING PERIODS ON FOOD INTAKE, GROWTH RATE, COMPENSATORY GROWTH, AND EFFICIENCY OF FEED UTILIZATION IN BLUE TILAPIA (Oreochromis aureus) REARED IN BRACKISH WATER PONDS Setyawan, Priadi; Robisalmi, Adam; Listiyowati, Nunuk; Dewi, Raden Roro Sri Pudji Sinarni; Imron, Imron
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Blue tilapia (Oreochromis aureus) has known as euryhaline species refers to wideÂ range of salinity tolerance. The pure population or hybrid with other strain of tilapiaÂ can be a good candidate in marginal coastal land use. Tilapia is the most importantÂ aquaculture species in Indonesia. Refers to FAO 2010, Indonesia is the third biggestÂ of tilapia production after China and Egypt. One of the main problems in aquacultureÂ is their feed as the major cost in fish farming. Various techniques had carried out toÂ reduce of feed cost such as improving fish quality, sex reversal and feed management.Â This research aimed to determine of compensatory growth in tilapia as one techniqueÂ in feed management. Fry obtained from natural spawning in freshwater pond.Â Acclimatization in 20 ppt made in aquarium for 4 days after one month reared inÂ hapas. Fish reared in 2 m x 1 m hapas with five treatments and three replications. ThisÂ research had conducted at brackish water pond in Yogyakarta. The treatments is A:Â one day fasting and six days feeding (1/6), B: 2/5, C: 3/4, D: 4/3 and E is control.Â Results showed that the biggest of average weight gain is treatment of E (68.36 g)Â followed by A (66.38 g), B (62.44 g), C (43.56 g), and D (27.30 g) respectively. One-wayÂ ANOVAs analysis with 95% of interval confidences continued with Tukeyâs PairwaiseÂ comparison showed that nothing significant different between E, A, and B. The biggestÂ of daily growth rate and specific growth rate is E (0.75 g/day and 4.68 %bw/day)Â followed by A (0.73 g/day and 4.65 %bw/day); B (0.69 g/day and 4.58 %bw/day); CÂ (0.48 g/day and 4.18% bw/day) and D (0.29 g/day and 3.65% bw/day) respectively.Â Survival rate of A is 73.00% followed by E. 72.00%, C. 71.00%, D. 69.69%, and B. 67.00%Â respectively. Feed conversion ratio of D is 0.83 followed by C (0.87), B (0.98), A (1.16),Â and E (1.41). Food efficiency ratio of D is 127.06% followed by C (118.75%), B (106.09%),Â A (88.87%), and E (73.38%). These results indicate that fasting of one day and two daysÂ has no significant effect on fish growth. Treatment A and B is the better treatment forÂ fish culture refers to the better value of FCR, FER, and total weight gain.

IDENTIFIKASI ZIGOSITAS IKAN LELE (Clarias gariepinus) TRANSGENIK F-2 YANG MEMBAWA GEN HORMON (PhGH) DENGAN MENGGUNAKAN METODE REALTIME-qPCR Marnis, Huria; Iswanto, Bambang; Suprapto, Romy; Imron, Imron; Dewi, Raden Roro Sri Pudji Sinarni
Jurnal Riset Akuakultur Vol 11, No 1 (2016): (Maret 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Produktivitas ikan budidaya dapat ditingkatkan melalui teknologi transgenesis. Populasi ikan lele transgenik cepat tumbuh telah dihasilkan dan karakter biologisnya telah diketahui. Namun informasi zigositas ikan lele transgenik perlu ditelaah lebih lanjut. Penelitian ini bertujuan untuk mengidentifikasi zigositas ikan lele transgenik F-2. Zigositas ikan lele transgenik diidentifikasi dengan menggunakan metode real-time qPCR (RT-qPCR) dan uji progeni. Identifikasi zigositas melalui uji progeni, dilakukan dengan mendeteksi transgen (PhGH) pada individu-individu F-3 hasil persilangan transgenik F-2 dengan non-transgenik. Hasil penelitian menunjukkan bahwa zigositas pada ikan lele transgenik F-2 dapat diidentifikasi dengan menggunakan metode RT-qPCR. Semua ikan transgenik F-2 adalah heterozigot, dengan nilai 2-ïïCt yang hampir sama tiap individu F-2, yaitu berkisar 0,80-0,99. Identifikasi zigositas dengan metode RT-qPCR menunjukkan hasil yang sama dengan uji progeni, semua transgenik F-2 tidak menghasilkan 100% anakan F-3 positif transgen. Pada uji progeni, transmisi transgen pada penelitian ini tidak mengikuti hukum segregasi Mendel, dengan kisaran sebesar 5%-40%.Fish farming productivity can be increased by transgenesis technology. On the previous study, transgenic African catfish population fast growing has been produced and its biological characters has been known. However information of transgenic zygosity of catfish should be examined. The aim of this study was to identify the zygosity of F-2 transgenic African catfish. The zygosity of F-2 transgenic was identified by real time-qPCR (RT-qPCR) method and progeny test. Further, identification of zygosity F-2 transgenic African catfish was confirmed by progeny test, while F-2 transgenic African catfish was mated with non-transgenic. Identification of zygosity F-2 transgenic was conducted by detection PhGH gene (transgene) in F-3 transgenic African catfish population. Transgene transmission was evaluated by PCR method. The result showed that the zygosity F-2 transgenic African catfish could be identified by RT-qPCR method. All F-2 transgenic African catfish were heterozygous, where as the 2-ïïCt value was almost same for all individual, which ranges from 0.80 to 0.99. The result of zygosity identification using RT-qPCR method was as same as that of progeny test. In the progeny test, transgene transmission in this study was non-Mendelian segregation, with ranges of 5%-40%.

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