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Ni Made Ritha Krisna Dewi
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Vaksin Polivalen Untuk Mencegah Penyakit Flu Burung (POLIVALEN VACCINE TO PREVENT BIRD FLU DISEASES) Suartha, I Nyoman; Wirata, I Wayan; Putra, I Gusti Ngurah Narendra; Dewi, Ni Made Ritha Krisna; Anthara, I Made Suma; Wibawan, I Wayan Teguh; Mahardika, I Gusti Ngurah Kade
Jurnal Veteriner Vol 13, No 2 (2012)
Publisher : Jurnal Veteriner

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This study was carried out to determine the use of bird flu polyvalent vaccines containing two or threeor more virus isolates representating of circulating viruses in the region. Three seed isolates of avianinfluenza H5N1 virus were used in this experiment. The isolates were Chicken/Denpasar/Unud-01/2004,Chicken/Klungkung/Unud-12/2006, and Chicken/Jembrana/Unud-17/2006. The seeds were inactivatedusing 0.01% formaldehide than mixed (AI3G) alumunium hidroxide adjuvant and then injectedintramuscularly to Isa Brown layer chicken at 3 weeks of age and repeated at the age of 5 weeks. The doseof each seed virus was 27 HA units. Sera were collected at one and two weeks after the second vaccination.The result showed that the arithmetic meant titer (AMT) of sera that tested with homologous isolate washigher than the test using a heterologous isolates, in the standard haemaglutination inhibition (HI) assay.The mixed AI3G vaccine produced a uniform AMT against the constituent isolates, while vaccines withindividual isolate yielded a lower and more variation in AMT. Further experiments using a commercialhomologous H5N1 and heterologous H5N2 commercial vaccines has resulted AMT that 1-4 log lower thanAI3G vaccine. It is concluded that polyvalent vaccine with field seed isolates is recommended to be appliedin the poultry farm in Indonesia.
Survei Penyakit Porcine Reproductive and Respiratory Syndrome pada Peternakan Babi di Bali (SEROLOGICAL SURVEILLANCE OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME IN PIGGERIES IN BALI) Suartha, I Nyoman; Anthara, I Made Suma; Wirata, I Wayan; Sari, Tri Komala; Dewi, Ni Made Ritha Krisna; Narendra, I Gusti Ngurah; Mahardika, I Gusti Ngurah
Jurnal Veteriner Vol 14, No 1 (2013)
Publisher : Jurnal Veteriner

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This study aimed to determine the presence and burden of Porcine Respiration and ReproductiveSyndrome (PRRS) virus in pig farms in Bali. A total of 305 sera samples were collected from 10 intensivepig farms and backyard piggeries located in eight districts, in Bali. The PRRS antibody and the virus wasdetected using enzyme linked immunosorbent assay (ELISA) and transverse reverse polymerase chainreaction (RT-PCR), respectively. The results showed that, generally the average percentage of positiveswine anti-PRRS antibody was 13.4%, 14.3%,  and 11.7% in the backyard farms and commercial farms,respectively. Whereas, the detection rate of PRRS virus was 8.9% (15.3% and 5.6% in the backyard farmand commercial farms, respectively). It was concluded that PRRS virus is endemic in pigs, in Bali.Vaccination, management, biosafety, and quarantine  should be implemented to prevent the economicloss due to PRRS.