Iswari Saraswati Dewi
Balai Besar Bioteknologi dan Sumberdaya Genetika Pertanian Jl. Tentara Pelajar No. 3A Bogor 16111, Indonesia

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Deteksi Dini Toleransi Padi Hibrida terhadap Kekeringan menggunakan PEG 6000 Afa, La Ode; Purwoko, Bambang Sapta; Junaedi, Ahmad; Haridjaja, Oteng; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 41, No 1 (2013): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this study was to determine a selection method at early stage for drought tolerant hybrid rice. Theexperimental design was split plot with 3 replications. In the first experiment the main plot was concentration of PEG 6000consisting of control and concentration 25% of PEG 6000. The subplots were hybrid genotypes/varieties, i.e. BI485A/BP3,BI485A/BP5, BI485A/BP10, BI485A/BP12, BI485A/BP15, BI599A/BP5, BI599A/BP15, BI665A/BP6, Maro, Hipa 8, IR64and Limboto. In the second experiment the main plot was drought stress level which consisted of control and drought stressat 60% field capacity. The subplots were hybrid genotypes/varieties used in the first experiment. The results showed that PEG 6000 could be used to detect drought tolerant genotypes at early stage. Seedling dry weight was the major character for selecting drought tolerant genotypes. Genotypes BI485A/BP15 and BI559A/BP15 are tolerant to drought and can potentially be grown in rainfed lowland.Keywords: drought tolerant, early selection, hybrid rice, polyethylene glycol
Konservasi In Vitro Pamelo (Citrus maxima (Burm.) Merr.) melalui Pertumbuhan Lambat Tyas, Kartika Ning; Susanto, Slamet; Dewi, Iswari Saraswati; Khumaida, Nurul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 41, No 1 (2013): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Indonesia is one of the countries which have abundant germplasm of pummelo (Citrus maxima (Burm.) Merr.). Thepummelo germplasm must be conserved to prevent its extinction due to biotic and abiotic stresses. In vitro conservationusing slow growth technique can be considered as an alternative of ex-situ conservation. Two experiments were conductedto obtain suitable medium to conserve pummelo in vitro. The first experiment was conservation using modified concentrationof MS and sucrose. MS medium concentrations were 1/2MS and MS, while sucrose concentrations were 0, 1, 2 and 3%. Thesecond experiment was conservation using osmoticum and retardant in MS medium. There were six combinations of MSmedium supplemented with osmoticum and retardant, i.e. MS + sucrose 3%, MS + sucrose 3% + paclobutrazol 7.5 ppm, MS+ sucrose 3% + paclobutrazol 15 ppm, MS + sorbitol 2%, MS + sorbitol 2% + paclobutrazol 7.5 ppm, MS + sorbitol 2% +paclobutrazol 15 ppm. The results showed that reducing MS medium and sucrose consentration decreased leaf number andshoot length but increased root number and length. The combination of osmoticum and retardant reduced shoot length, leaf number, root number and length. Based on the planlet visual and inhibition of growth through the decrease of leaf number, shoot and root length, the best medium to preserve pummelo was MS + sorbitol 2% + paclobutrazol 7.5 ppm.Keywords: Citrus maxima, osmoticum, preservation, retardant, slow growth
Karakter Morfologi dan Kimia Kultivar Pamelo (Citrus maxima(Burm.) Merr.) Berbiji dan Tanpa Biji Rahayu, Arifah; Susanto, Slamet; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 1 (2012): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this study was to evaluate morphological and chemical characteristics of seeded and seedless pummel fruit. The study was carried out during April 2009 to July 2010. The pummelo fruits were harvested from the production center of Sumedang, Pati, Kudus, Magetan, Bireun (Aceh) and Pangkep (South Sulawesi). Some of seeded cultivars have spherical and ellipsoid form, while the seedless one shown pyriform shape. The edible portion of the fruits (juice vesicles) mainly affected by peel thickness and fruit shape. The edible portion of seedless cultivars (57.22%) were not sigificantly different with seeded (57.07%) and potentially seedless cultivars (59.35%). Generally fruit taste of seedless cultivars were sweet to sweet-bitter, meanwhile seeded and potentially seedless pummelo cultivars have sour-sweet. Pummelo juice taste was affected by TSS (total soluble solids), TA (titratable acidity), TSS/TA ratio and naringin content. Seedless pummelo cultivars have higher pH, TSS, TSS/TA ratio, vitamin C and naringin, but lower in TA content than seeded and potentially seedless fruit juice. ‘Jawa 1’ and ‘Bali Merah 2’ pummelos can be further developed as seedless superior cultivars. Keywords: fruit shape, edible portion, TSS/TA, naringin, vitamin C
Konstribusi Akumulasi Silikat, Nitrogen dan Aluminium terhadap Ketenggangan Aluminium dan Ketahanan terhadap Penyakit Blas pada Padi Gogo Bakhtiar, ,; Purwoko, Bambang Sapta; Trikoesoemaningtyas, ,; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 3 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Aluminum (Al) toxicity and blast disease are the most important yield-limiting factors for upland rice production in acid soils. The objective of this experiment was to examine the contribution of accumulation of Silicate (Si), Nitrogen (N) and Al in plant tissue on Al tolerance and blast disease resistance in upland rice. The experiment was arranged in a split-split plot design with 2 replications. Main plots were randomly assigned to blast treatment (control and inoculation to blast fungi). Subplots were assigned to control box (lime 1.5 AlEC) and another acid soils (no lime) box and sub-subplots were assigned to the tested genotypes. The result of the experiment showed that leaf blast disease resistance in rice cannot be solely explained by Si or N content in shoot tissue. The resistant to leaf blast disease might be attributed by high ratio Si/N weight in shoot. Al tolerance was ascribed by low reduction in root growth, high shoot dry weight, high Si content in shoot, and as well as high of  Si/Al ratio in root.   Key words:  Al-tolerance, blast disease, upland rice, Si/Al ratio
Potensi dan Stabilitas Hasil, serta Adaptabilitas Galur-galur Padi Gogo Tipe Baru Hasil Kultur Antera ., Purbokurniawan; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 42, No 1 (2014): JURNAL AGRONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

ABSTRACTThe objectives of the research were to obtain information on yield potential, adaptability and stability of the upland rice lines. Ten lines and two cultivars were planted at five different locations in November 2010-March 2011. In each location, the experimental design was randomized complete block design with four replications. Observation was done on grains weight per hectar. The results showed that FM1R-1-3-1 achieved the highest productivity (5.65 ton ha-1). Genotype FG1R-36-1-1 was classified as stable genotype by four yield stability analysis as followed: Francis-Kannenberg, Finlay-Wilkinson, Eberhart-Russell and AMMI. Genotypes FG1-70-2-1, FG1R-30-1-5, FG1R-30-1-4 and FG1R-30-1-3 were classified as stable genotypes by three yield stability analysis: Finlay-Wilkinson, Eberhart-Russell and AMMI. AMMI  analysis showed that FG1-6-1-2, FG1-65-1-2, FG1R-30-1-1, FM1R-1-3-1, Fat-4-1-1, Situ Bagendit and Towuti as specific genotypes in certain environment.Keywords: dynamic, new plant type, specific, static, yield
Heritabilitas, Karakterisasi, dan Analisis Clustergram Galur-galur Padi Dihaploid Hasil Kultur Antera Anshori, Muhammad Fuad; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, Sintho Wahyuning; Suwarno, Willy Bayuardi; Safitri, dan Heni
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 46, No 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

Doubled haploid rice formation through anther culture becomes one of the alternatives in the plant breeding. The lines need to be characterized, clustered, and selected. This study aimed at determining heritability, characters, and cluster of doubled haploid lines obtained from anther culture. This experiment was conducted at IPB Rice Field Experiment Station, Darmaga, Bogor from October 2016 until January 2017. The experiment was managed according to randomized complete block design (RCBD) with 3 replications and genotype as the single factor treatment. Genotypes used were 60 genotypes consisting of 56 doubled haploid lines and 4 control varieties. The controls were Ciherang, Inpara 5, Inpari 29, and Inpari 34 Salin Agritan. The results showed that all characters have significant effect and high heritability (above 60%), so they could be used as a selection character. The tested genotypes could be grouped into three main groups. The first group consisted of 11 genotypes, the second group consisted of 44 genotypes, and the third group consisted of 5 genotypes. The main group determinant characters were productive tillers, percentage of filled grain number, plant height, panicle length, and weight of 1,000 grains. The number of filled grain and productive tillers can be used as selection characters together with yield potential.
Mapping of Resistance Genes to Brown Planthopper in Untup Rajab, an Indonesian Local Rice Variety Yunus, Muhamad; Damayanti, Diani; Dadang, Ahmad; Warsun, Ahmad; Satyawan, Dani; Kusumanegara, Kusumawaty; Dewi, Iswari Saraswati; Sutrisno, Sutrisno; Husin, Bahagiawati Amir
Jurnal AgroBiogen Vol 14, No 2 (2018): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Abstract

Brown planthopper (BPH) is a major rice pest in Indonesia. The most economical and effective approach to control the insect pest is by using resistant varieties. Exploring for resistance genes is, therefore, a prerequisite for effective breeding program for BPH resistance. This study aimed to map BPH resistance genes in Untup Rajab, an Indonesian local rice variety. Genetic map was constructed using an F2 population from a cross between TN-1 and Untup Rajab, and SNP markers from RiceLD SNP Chip. Phenotyping was performed using bulk seedling test on F2:3 seedlings against two BPH populations, i.e. X1 and S1. Four QTLswere identified on chromosomes 5, 6, 8, and 11 with PVE values of 7.63%, 9.40%, 17.66%, and 3.05%, respectively. Relatively normal distribution of resistance phenotype and the relatively low PVE values indicate that Untup Rajab has a quantitative resistance to BPH with two different resistance loci identified for each BPH test population. The QTL on chromosome 8 overlaps with OsHI-LOX gene, which is associated with resistance to BPH, and adjacent to another QTL for resistance to green leafhopper. The QTL on chromosome 6 was found near OsPLDα4 and OsPLDα5 genes which are related to BPH resistance. Meanwhile, the QTL intervals on chromosome 5 and 11 did not overlap with any known BPH QTLs or genes, which make them attractive candidates for novel BPH resistance gene discovery.
Karakterisasi Sifat Agronomi Tanaman Padi Beras Merah Dihaploid Berpotensi Hasil Tinggi Diperoleh melalui Kultur Antera Antera ,, Mawaddah; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Wirnas, dan Desta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 46, No 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

Observation and identification of rice germplasm with advanced traits are important activities in the process of obtaining a variety of rice because every genotype will show unique characters. Genotype derived from breeding program as anther culture need to be characterized to identify the doubled haploid lines that are potential to be developed in red rice breeding. This research was aimed to obtain agronomic characters of doubled haploid lines of red rice. Materials used were 59 genotypes, i.e., 57 doubled haploid (DH0) lines with red grain and 2 check varieties (Inpari 24 and Aek Sibundong) as control. The experiment used a randomized complete block design with three replications. The results exhibited there was agronomic variation among DH0 of red rice lines. Variance analysis showed that genotype affected most characters, except for grain dry weight per hill. Plant height, number of productive tiller, harvesting age, panicle length, number of grains per panicle, and number of filled grain per panicle can be used as a reference for effective selection to develop high productivity of red rice variety.
Penentuan Indeks Seleksi Toleransi Kekeringan Galur Dihaploid Padi Sawah Tadah Hujan pada Fase Perkecambahan Akbar, Miftahur Rizqi; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, dan Willy Bayuardi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 46, No 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

Rainfed rice tolerant to drought and high yielding would be an alternative to rainfed lowland areas prone to drought stress. Selection in the early phase of plant growth will accelerate the effort to obtain rainfed varieties. The objective of this experiment was to identify characters that significantly affect the selection of drought tolerance using PEG 6000 concentration of 25% in the germination phase. The experiment was conducted in greenhouse of ICABIOGRAD, Bogor, using a factorial randomized complete block design with three replications. The first factor was 32 rice genotypes while the second factor was 2 levels of PEG 6000, i.e., concentrations of 0 and 25%. The results showed that the correlation analysis and principal component analysis obtain the important characters namely the germination percentage, seminal root length, and the dry weight of seminal roots. Based on discriminant analysis, the index matching value of 96.77% was suitable to distinguish tolerant and sensitive genotypes using PEG 6000 concentration of 25% in the germination phase.
Skrining Awal Toleransi Galur-galur Dihaploid Padi Gogo terhadap Cekaman Kekeringan pada Stadia Bibit Kartina, Nita; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Wirnas, Desta; Nindita, dan Anggi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 47, No 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

Development of doubled haploid lines of upland rice through anther culture is one way to obtain superior upland rice varieties tolerant to drought. The objectives of this research were to determine the response of doubled haploid lines to drought stress at seedling stage and to select tolerant doubled haploid lines. The research was conducted at a screenhouse of Muara Research Station, Indonesia Center of Rice Research (ICRR) from November to December 2017. Fourteen doubled haploid lines and 4 check varieties, namely Inpago 10 and Limboto, Salumpikit (drought tolerant check) and IR 20 (drought sensitive check) were used in this study. The experiment was arranged in a randomized complete block design with three replications. The observations were conducted on leaf rolling, leaf drought, and recovery ability. Based on Friedman nonparametric analysis, HR-5-7-1-1 and HR-7-15-2-1 had moderate response with estimated median values for leaf rolling of 3.44 and 3.00, respectively. Three doubled haploid lines namely HR-2-27-2-7, HR-2-34-1-3 and HR-7-15-2-1 had moderate response to drought with estimated median value of leaf drought of 3.56. Nine lines of doubled haploid had tolerant response with estimated median value of recovery ability of 1.00-1.56. Based on weighted selection index, nine doubled haploid lines with positive and high selection index were identified; however, only two lines (HR-7-15-2-1 and B3-2) had selection index higher than Inpago 10 and Limboto.Keywords: drought tolerance, leaf rolling, recovery, weighted selection index