Intan Kusuma Dewi

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Perbandingan Daya Hambat Ekstrak Etanol Dengan Sediaan Sirup Herbal Buah Belimbing Wuluh (Averrhoa bilimbi L.) Terhadap Pertumbuhan Shigella dysenteriae In Vitro Dewi, Intan Kusuma; Joharman, Joharman; Budiarti, Lia Yulia
Jurnal Berkala Kedokteran Vol 9, No 2 (2013): September 2013
Publisher : Pendidikan Dokter Universitas Lambung Mangkurat

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ABSTRACT: Sour carambola (Averrhoa bilimbi, L) fruit has antibacterial effect to Shigella dysenteriae. Shigella dysentriae is Gram-negative bacteria caused shigellosis and bloody diarrhea in human. Sour carambola can be used as extract and herbal syrup. This research aims to compare the inhibitory effect between ethanol extract and herbal syrup of sour carambola fruit to against Shigella dysenteriae in vitro. The concentration of ethanol extract and herbal syrup were 60 %,70%, 80% and 90%. Antibacterial effect was tested by Kirby- Bauer diffusion method on Mueller Hinton media and measure the inhibitory zone of  Shigella dysenteriae. The result of inhibitory zone was tested by Kruskal-Wallis and Mann-Whitney post hoc tests with 95% significance level showed  that ethanol extract and herbal syrup of sour carambola showed the differences in concentration of 60% (p < 0,05). The phytochemical screening result showed that ethanol extract of sour carambola contains  flavonoid, saponin, alkaloid and steroid. Keywords: Averrhoa bilimbi, L., ethanol extract, herbal syrup, Shigella dysenteriae ABSTRAK: Buah belimbing wuluh (Averrhoa bilimbi, L.) terbukti memiliki aktivitas antibakteri terhadap Shigella dysenteriae. Shigella dysenteriae merupakan bakteri penyebab shigellosis atau disentri basiler. Buah belimbing wuluh dapat digunakan dalam bentuk ekstrak dan sirup. Penelitian ini bertujuan untuk mengetahui adanya perbedaan daya hambat antara ekstrak etanol dengan sediaan sirup herbal buah belimbing wuluh dalam menghambat pertumbuhan Shigella dysenteriae in vitro. Konsenterasi ekstrak etanol dan sirup herbal buah belimbing wuluh yang digunakan adalah 60%, 70%, 80% dan 90%. Uji antibakteri dilakukan menggunakan metode difusi Kirby-Bauer dengan media Mueller- Hinton dan dilakukan pengukuran zona hambat Shigella dysenteriae. Hasil zona hambat yang terbentuk diuji menggunakan Kruskal Wallis dan post hoc Mann Whitney dengan tingkat kepercayaan 95% dan menunjukkan bahwa ekstrak etanol dan sediaan sirup herbal buah belimbing wuluh memiliki perbedaan bermakna dalam menghambat Shigella dysenteriae pada konsenterasi 60% (p<0,05). Hasil skrining fitokimia ekstrak etanol buah belimbing wuluh menunjukkan adanya kandungan flavonoid, saponin, alkaloid dan steroid.  Kata-kata kunci: Averrhoa bilimbi, L., ekstrak etanol, Shigella dysenteriae, sirup herbal
Folia Medica Indonesiana Vol 52, No 4 (2016): OCTOBER - DECEMBER 2016
Publisher : Faculty of Medicine, Universitas Airlangga

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NT-proBNP is an inactive fragment of BNP secreted by stretched ventricle as response to wall stress in patients with heart failure. As a specific cardiac marker, elevated NT-proBNP correlates well with heart failure severity. The principle of heart failure therapy is modulation on neurohormonal activation. ARB can modulate neurohormon on RAA system, that result in decreasing NT-proBNP level and favorable outcomes. Reduction in NT-proBNP more than biologic variability (> 25%) shows a therapy response.This study was to analyze change of NT-proBNP after ARB therapy in ambulatory HF patients. This observational prospective study was carried from September to December 2015. Blood sampling was performed on patients who meet the inclusion criteria of the study at first visit and after 2 months therapy. NT-proBNP was measured by IMMULITE® as primary parameter and creatinin as secondary parameter. There are 14 patients met the inclusion criteria of the study (11 males and 3 females). ARB therapy used in patients were Valsartan (64%), Telmisartan (22%) and Candesartan (14%). After 2 months ARB therapy, a decrease in level of NT-proBNP with initial median 3092.5 (216 – 32112) pg/ml to 2135.5 (350 – 16172) pg/ml respectively were statistically significant (p=0.003). And the secondary parameter creatinin serum convert to eGFR shows a change in eGFR with initial median 73.33 (37.05 – 266.68) ml/minute to 81.04 (39.31 – 167.02) ml/minute respectively were statistically not significant (p=0.657). There were 7 patients (50%) have a decrease > 25%. In this study, we found that ARB therapy can change NT-proBNP level significantly after 2 months therapy.