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CA2+ INTRASELULER TERLIBAT DALAM MEKANISME PEMBUKAAN STOMATA AKIBAT PENGARUH AUXIN PHARMAWATI, MADE; DEFIANI, MADE RIA; ARPIWI, Ni LUH
Jurnal Biologi Vol XII, No 1
Publisher : Jurnal Biologi

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Research was done to elucidate the role of Ca2+ intracellular in stomatal movement induced by auxin. Two types of auxin (IAA and NAA) increased stomatal opening and the increase was concentration dependent. The addition of EGTA, Ruthenium red and Procaine as the modulator of Ca2+ concentration , inhibited stomatal opening induced by auxin. EGTA and procaine significantly inhibited somatal opening at 100 uM and 1 mM. Ruthenium red worked at lower concentrations which were 10 uM, 50 uM and 100 uM on LAA-induced stomatal opening, while on NAA-induced stomatal opening, Ruthenium red had an effect at 10 uM, 50 uM, 100 uM dan 1 mM. It is suggested that Ca2+ involves in stomatal opening induced by LAA and NAA as signaling agent.
PENGARUH SUHU DAN LAMA PENYIMPANAN TERHADAP KANDUNGAN VITAMIN C PADA CABAI RAWIT PUTIH (CAPSICUM FRUSTESCENS) RACHMAWATI, RANI; DEFIANI, MADE RIA; SURIANI, NI LUH
Jurnal Biologi Vol XIII, No 2
Publisher : Jurnal Biologi

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The effect of temperature and length of storage on vitamin C concentration of chili (Capsicum frustescens) was observed on this study. Samples were taken from farmer’s field around Gianyar Regency of Bali. The chilies were treated with temperature such as 10ºC, 20ºC, and 29ºC (room temperature) and stored for 5, 10, and 15 days. Jacobs method was applied for vitamin C determination. The highest vitamin C content was obtain from control (59,9 mg/100 ml). On the other hand, the lowest was from chili that stored at room temperature for 15 days (23,6 mg/100 ml). For weight loss, the highest of loss was from chili which stored at room temperature for 15 days.
PERTUMBUHAN VEGETATIF DAN PRODUKTIVITAS BERBAGAI KULTIVAR BROKOLI (Brassica oleracea L. var. italica Plenck.) INTRODUKSI DI DESA BATUR, KECAMATAN KINTAMANI, KABUPATEN BANGLI, BALI Raleni, Ni Kadek; Defiani, Made Ria; Astarini, Ida Ayu
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Broccoli (Brassica oleracea L. var. italica Plenck.) is a vegetable crop belongs to Brassicaceae family.  Broccoli has high nutrition, high in fiber and contains isotiacyanate that has anticancer activity.  Broccoli market in Indonesia, particularly in modern supermarkets, increases 15-20% per year, however, productivity was still low, therefore effort to increase broccoli production in Indonesia need to be investigated. Field trial was conducted at Batur Village, Kintamani District, Bangli Regency, Bali, to find out cultivars that were adapted in tropical region. Introduced cultivars being trialed were ‘Alborada’,‘Belstar’, ‘Fiesta’, ‘Sarasota’, ‘Bay Meadows’, ‘Castle Dome’, ‘Liutenant’, ‘Iron Man’, ‘Marathon’, ‘Green Gold’, ‘Imperial’, ‘Green Magic’ and ‘Lucky’ as control.  Variable observed were vegetative growth, curding period, and productivity of each cultivar.  This study employed Randomized Completely Block Design with 3 replicates (plots) and 8 plants each plot.  Data were analyzed using ANOVA (Analysis of variance) on Costat program, followed by Duncan’s Multiple Range Test (DMRT) on 1% level.  Results show that each cultivar varied in adaptability in tropical region. ‘Castle Dome’ has the highest productivity, while ‘Fiesta’ was the lowest. Keywords: Brassica, field trial, cultivar
EMBRIOGENESIS PADA TANAMAN TEBU (Saccharum sp.) VARIETAS BULULAWANG DENGAN TEKNIK KULTUR MIKROSPORA Anitasari, Septarini Dian; Sari, Dwi Nur Rikhma; Astarini, Ida Ayu; Defiani, Made Ria
AGRITROP Vol 16, No 2 (2018): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.727 KB) | DOI: 10.32528/agritrop.v16i2.1813

Abstract

Tanaman tebu adalah keluarga poace yang berpotensi menghasilkan nilai ekonomi tinggi karena manfaatnya sebagai produk unggulan dalam menghasilkan gula serta bioetanol yang banyak digunakan oleh masyarakat luas. Untuk itu perlu upaya pemuliaan tanaman yang cepat untuk memenuhi kebutuhan pasar. Teknik kultur mikrospora merupakan aplikasi bioteknologi yang efisien dalam menghasilkan tanaman haploid. Teknik ini belum berhasil diaplikasikan pada tanaman tebu sehingga dalam penelitian ini bertujuan untuk memicu embriogenesis mikrospora tebu dengan aplikasi kultur mikrospora pada varietas bululawang. Metode yang digunakan yaitu dengan stress perlakukan perendaman mannitol 0.3M sebelum isolasi kultur pada lama perendaman yang berbeda. Analisis data menggunakan uji anova dan uji duncan. Hasil yang diperoleh yaitu Perlakuan stress yang diberikan berhasil memicu embriogenesis mikrospora dengan lama perendaman anther optimum pada 7 hari pada mannitol 0.3M
FLORAL DIVERSITY IN MINCIDAN VILLAGE, KLUNGKUNG, BALI TO SUPPORT ECOTOURISM Defiani, Made Ria; Kriswiyanti, Eniek
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

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  Keanekaragaman flora menjadi kekayaan hayati bagi kehidupan di alam. Penelitian bertujuan mengeksplorasi jenis dan manfaat tumbuhan di sekitar desa Mincidan agar dapat digunakan sebagai dasar pendidikan dan pengembangan ekowisata.   Metode deskriptif kualitatif dengan survei dan wawancara dengan penduduk desa dilakukan untuk melengkapi data yang dapat dikembangkan dalam rencana konservasi tanaman.  Ragam tanaman yang dijumpai di sepanjang rencana jalur trekking sepanjang 1,5 km tergolong pada tanaman obat, hias, buah, dan upakara.   Berdasarkan hasil survey, dapat diidentifikasi 102 jenis tumbuhan, 71 genera yang termasuk dalam 38 familia. Tanaman tersebut dapat dimanfaatkan untuk bahan upacara keagamaan dan obat tradisional.
PERTUMBUHAN ANGGREK Vanda helvola PADA MEDIA YANG DIPERKAYA JUS TOMAT Muharyati, Yully; Defiani, Made Ria; Astiti, Ni Putu Adriani
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2015.v02.i02.p03

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Vanda is one of the most cultivated orchid species.  In nature, Vanda is endangered because it was exploited in large quantities. Orchid genetic viability can be maintained by embryo culture. The study aimed to investigate the growth of Vanda helvola seeds during 12 weeks after cultured. Media Murashige and Skoog (MS) added with tomato juice 100 g/L was used as culture medium. The results showed that embryo could grow on MS medium on stadium 6th. Protocorm started to form at 9 weeks after cultured. At 12 weeks, percentage of protocorm formed reach almost 24.1%. Tomato juice improved the growth of protocorm of  V.helvola. Key words: Medium, protocorm, Vanda helvola
INDUKSI PERTUMBUHAN TUNAS DARI EKSPLAN ANGGREK Dendrobium Heterocarpum Lindl. DENGAN PEMBERIAN HORMON ZEATIN DAN NAA Febryanti, Ni Luh Putu Kayika; Defiani, Made Ria; Astarini, Ida Ayu
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p07

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Dendrobium heterocarpum Lindl. is a native orchid in Indonesia. Its population continue to decline due to unsustainable exploitation. One solution to increase its population is via in vitro technique propagation. This research aimed to determine effect of hormone addition (Zeatin and NAA) to induce shoot multiplication of D. heterocarpum Lindl. grown in Murashige and Skoog (MS) basic medium. The research was conducted in Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Science, Udayana University from November 2015 until March 2016. The experiment employed Completely Randomized Design (CRD) with 20 combinations of treatments, with three replicates each treatment. The treatment werer addition of Zeatin and NAA, with five concentrations of Zeatin (0, 0.1, 0,5, sweet corn and hybrids corn as the natural Zeatin); four NAA concentrations (0, 0.05, 0.1 and 0.5). Variable recorded include such as the number of shoots, roots and explant’s vigor. The quantitative data which include number of leaves and shoots after 12 weeks, were analyzed using Analysis of Variance (ANOVA); and if significantly different, tested further using Duncan Multiple Range Test (DMRT) at 5% level. Z5N3 medium revealed significantly different on amount of leaves and shoot height and also the best medium on amount of roots. Best hormone combination that can induce multiplication of new shoots are medium Z4N3 and Z5N1.
Pollen Viability and Microspore Culture in Three Broccoli Cultivars (Brassica oleracea L. var. italica Plenck) Anitasari, Septarini Dian; Astarini, Ida Ayu; Defiani, Made Ria; ., Made Pharmawati; Prayantini, Dian Catur
Biota Vol 5 No 2 (2019): Jurnal Biota 2019
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

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Broccoli is a high value vegetable crop in Indonesia, however production is low due to limited number of suitable cultivars, so, breeding hybrid broccoli for warm climate is important.  The first step in hybridization is providing homozygote parent plants which can be done efficiently via microspore culture.  The objectives of this study were to determine 1) bud size that produce uninucleate microspore stage appropriate for culture; 2) pollen viability, 3) microspore development, in three broccoli cultivars (?BL 10001?, ?Royal Green? and ?Green Magic?). Various bud size (1 ? 5 cm) was squashed and observed microscopically to determine bud size containing uninucleate microspore.  Pollen viability was determined by IKI staining and pollen germination method.  Chromosome number was counted on root tips using squash method with aceto-orcein stain. Various heat treatment schemes were conducted to induce microspreo development.  Result showed uninucleate microspore derived from 2-3 mm and 3-4 mm bud length of ?BL-10001? and ?Royal Green? was responsive for microspore development in culture.  Pollen viability varied among cultivars, 78-87% on IKI method and 15-16% on germination test. Microspore culture showed different embryogenesis response; pollen-like structure was produced by ?BL 10001?.