Articles

Identification of Local Melon (Cucumis melo L. var. Bartek) Based on Chromosomal Characters DARYONO, BUDI SETIADI; KUMALAWATI, DIAN ARUNI
HAYATI Journal of Biosciences Vol 18, No 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.74 KB) | DOI: 10.4308/hjb.18.4.197

Abstract

Bartek is one of local melon varieties mainly cultivated in Pemalang, Central Java. Bartek has three variations of fruits; Long-Green, Ellips-Green, and Yellow. Chromosome characterization of the Bartek was investigated to determine the genetic variation. The main purpose of this research was to determine the genetic characters of Bartek including chromosome number, mitosis, cell cycle, and karyotype. Squash method was used for chromosome preparation. The results showed that all of Bartek observed in this study have similar diploid (2n) chromosome number = 24. According to the total number of chromosome, Bartek is closer to melon than cucumber. The mitotic analysis exhibited that the Bartek has similar karyotype formula, 2n = 2x = 24m. Based on the R value of the three kinds of Bartek (R < 0.27), it indicated that three kinds of Bartek were considered to be originated from similar species and one of melon varieties (Cucumis melo L. var. Bartek).
Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in Melon DARYONO, BUDI SETIADI; WAKUI, KENJI; NATSUAKI, KEIKO T.
HAYATI Journal of Biosciences Vol 16, No 4 (2009): December 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1059.16 KB) | DOI: 10.4308/hjb.16.4.142

Abstract

Two random amplified polymorphic DNA (RAPD) markers linked to CMV-B2 resistance gene (Creb-2) in melon cultivar Yamatouri were cloned and sequenced to design sequence characterized amplified region (SCAR) markers for detection of CMV-B2 resistance gene (Creb-2) in melon. SCOPE14 derived from OPE-14 yielded a single DNA band at 541 bp, while SCAPB05 derived from APB-05, yielded a single DNA band at 1,046 bp, respectively. Segregation of SCOPE14 and SCAPB05 markers in bulk of F2 plants demonstrated that they were co-segregated with RAPD markers from which the SCAR markers were originated. Furthermore, results of SCAR analysis in diverse melons showed SCAPB05 primers obtained a single 1,046 bp linked to Creb-2 in resistant cultivars Sanuki-shirouri and Kohimeuri. However, SCOPE14 failed to detect Creb-2 in diverse melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to CMV-B2 by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.         Key words: Cucumis melo L., Creb-2, RAPD, SCAR
UJI ADAPTASI MULTIMUSIM KARAKTER FENOTIP KULTIVAR MELODI GAMA 3 (Cucumis melo L.): USAHA PENGUATAN INDUSTRI BENIH NASIONAL Sidiq, Yasir; Maryanto, Sigit Dwi; Daryono, Budi Setiadi
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.947 KB)

Abstract

Tanaman melon (Cucumis melo  L.) merupakan tanaman pertanian yang banyak dibudidayakan untuk konsumsi buahnya. Salah satu permasalahan dalam budidaya melon adalah benih yang diperoleh secara impor sehingga harganya relatif mahal. Perakitan kultivar MelodiGama 3 (MG3) merupakan strategi untuk mengurangi ketergantungan terhadap benih impor. Kultivar MG3 merupakan hasil persilangan antara kultivar ♀ MG1 dengan ♂ La-3 (tahun 2011). Tujuan penelitian ini untuk mengetahui kemampuan adaptasi kultivar MG3 terhadap kondisi lingkungan pada musim kemarau (tahun 2012) dan musim penghujan (tahun 2013) dan mengamati kestabilan kultivar MG3 di kedua musim tersebut di demplot milik Kebun Pendidikan Penelitian dan Pengembangan Pertanian (KP4) Universitas Gadjah Mada (UGM), Yogyakarta. Hasil yang diperoleh menunjukkan bahwa karakter melon MG3 dari penanaman musim penghujan umumnya lebih unggul daripada penanaman musim kemarau. Melon MG3 penanaman musim penghujan unggul pada karakter berat buah, keliling buah, ukuran buah, dan berat biji. Namun terdapat penurunan karakter unggul yaitu tingkat kemanisan (brix) dari 11,53% menjadi 9,98%. Hasil tersebut terjadi karena musim penghujan yang mempengaruhi kadar air dalam buah sehingga tingkat kemanisanya menurun. Kata kunci: MG3, Karakter Fenotip, Pemuliaan Tanaman
Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) FERNIAH, REJEKI SITI; DARYONO, BUDI SETIADI; KASIAMDARI, RINA SRI; PRIYATMOJO, ACHMADI
Microbiology Indonesia Vol 8, No 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.8.3.5

Abstract

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified  morphologically and molecularly as F. oxysporum.  
Pola Pewarisan Crest Ayam (Gallus gallus domesticus, Linnaeus 1758) Backcross Hasil Persilangan Ayam Mahkota dengan Ayam Kampung Daryono, Budi Setiadi; Puspita, Utin Elsya
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.22146/jsv.17884

Abstract

AbstrakMemelihara ayam hias merupakan salah satu kegemaran masyarakat Indonesia dan juga saranameningkatkan pendapatan. Salah satu ayam hias yang unik dan banyak menarik minat pecinta ayam hias adalah ayam Mahkota yang memiliki ciri khas bulu yang tersusun lebat di bagian kepala hingga menutupi mata. Jenis ayam hias tersebut umumnya memiliki kelemahan yaitu daya tahannya yang rendah terhadap penyakit. Ayam Kampung atau ayam buras merupakan ayam lokal asli Indonesia yang memiliki keunggulan yaitu memiliki ketahanan yang lebih tinggi terhadap penyakit dibanding ayam ras. Penggabungan keunikan crest dari ayam Mahkota dan ketahanan terhadap penyakit dari ayam Kampung dapat diwujudkan salah satunya melalui proses backcross (BC). Penelitian ini dilakukan dengan menyilangkan ayam F Mahkota betina hasil persilangan ayam 1 Mahkota dan ayam Kampung dengan ayam Mahkota jantan. Tujuan penelitian ini adalah untuk mempelajari pola pewarisan crest dan pertumbuhan bobot keturunan BC pada umur 7 minggu. Anakan yang dihasilkan diamati perkembangan crest dan pertambahan bobotnya selama 7 minggu, kemudian variasi fenotipnya dianalisis dengan chi square test. Hasil penelitian menunjukkan terdapat 5 kelas fenotip yang berbeda dari 16 individu keturunan BC yang menunjukkan gejala poligen dengan 2 gen yang mempengaruhi dan memenuhi pola pewarisan 1:4:6:4:1.
Stability Phenotypic Characters and The Scent of Gama Melon Parfum Cultivar Maryanto, Sigit Dwi; Ranis, Rini Etika; Daryono, Budi Setiadi
IPTEK Journal of Proceedings Series Vol 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v1i1.286

Abstract

Parfume is one of the cosmetic product and its use is consistent. High prices of cosmetic product cause dependence on imported product. Innovative research is needed to reduce dependence on imported product. Melon is one of the potential horticultural crops to be developed in plant breeding. Gama Melon Parfum cultivars has strong of fragnant so its potentially to be used as parfume material. Research on morphological characters to support of Gama Melon Parfum as superior cultivar. The research aims to determine the level of stability of phenotypic characters and aroma of melon Gama Melon Parfum cultivar. Research methods was cultivated Gama Melon Parfum in Kebun Pendidikan Penelitian dan Pengembangan Pertanian (KP4) UGM Yogyakarta. Furthermore, the phenotypic characters was observed consist of qualitative and quantitative characters. The methods of sampling used Randomize Complete Block Design (RCBD) and quantitative phenotypic characters were analyzed using software PKBT-STAT-2. The result of qualitative characters on Gama Melon Parfum cultivars has oblate shape, small size, yellow-orange skin of colour with 9-10 lobes and longitudinal, white of flesh colour, crunchy texture, bitter taste, zero score of nett (not netted), strong of fragnant, and has spesific qualitative characters called turbin. Quantitative characters on Gama Melon Parfume cultivars consist of average weight of 160 gram; circumference of 21,8 cm; horizontal diameter of 6,89 cm; vertical diameter of 6,44 cm; thick skin of 1 mm, thick flesh of 1,06 cm; the number of seed of 200-300; weight of 100 seed of 1,74 gram; harvest 63-65 day after cultivate; and 17 days of storage time.
Phenotypic Characters Stability of Melodi Gama-3 Melon (Cucumis melo L.) Cultivar in Rainy Season Based on Multilocation Test Daryono, Budi Setiadi; Hadi, Rizko; Sidiq, Yasir; Maryanto, Sigit Dwi
IPTEK Journal of Proceedings Series Vol 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v1i1.253

Abstract

The study was conducted to determine the stability of MG3 cultivar characters in rainy season based on multilocation test. Used materials were MG3 melon cultivar originating from the Laboratory of Genetics, Faculty of Biology, UGM, and Action 434, Glamour Sakata, MAI, and LADIKA melon cultivars as commercial cultivars. Multilocation test was conducted in March – July 2013 (rainy season) in 3 provinces i.e., East Java, Daerah Istimewa Yogyakarta, and Central Java. Observation of phenotypic characters was conducted at the Laboratory of Genetics, Faculty of Biology, Universitas Gadjah Mada. Qualitative phenotypic characters data of cultivar MG3 and cultivars Action 434, Glamour Sakata, MAI, and LADIKA were compared qualitatively, while the quantitative characters data were analyzed by balanced ANOVA using PKBTSTAT 2.0 software. The results showed that fruit weight, fruit girth, skin thickness, and fruit vertical and horizontal diameter (longitudinal section) has a very significant difference by looking at the interaction of cultivar x locations, while flesh thickness has no significant difference. Qualitative characters were discussed according to its uniformity in three location. It was concluded that melon can also give yield in rainy season. MG3 was unstable for fruit weight and girth, skin thickness, and fruit vertical and horizontal diameter characters, while stable for flesh thickness character. MG3 having uniformity for fruit shape, unripe and ripe skin color, netting intensity, flesh color, flesh texture, and water content characters from three cultivating location, while for netting distribution, cavity shape, flesh flavor, and flesh aroma characters, it have not uniform yet.
Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Daryono, Budi Setiadi; Aristya, Ganies Riza; Kasiamdari, Rina Sri
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers. &nbsp; Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5
Application of Multiplex RT-PCR for Detection of Cucurbit-infecting Tobamovirus Daryono, Budi Setiadi; Natsuaki, Keiko T.
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cucumber green mottle mosaic virus (CGMMV) and Kyuri green mottle mosaic virus (KGMMV) are seed borne viruses and they are also transmitted mechanically during agricultural practice and through water. Hence, these viruses have potential diseases widely distributed throughout the world. To detect different strains of CGMMV and KGMMV, several specific primers for each virus were designed for single and multiplex RT-PCR. The results of single and multiplex RT-PCR showed that CGMMV was detected in zucchini isolated in Bali-Indonesia, while KGMMV was detected both in zucchini isolated in Bali-Indonesia and Cucumis metuliferus isolated in Thailand. Furthermore, artificial co-infection of these two viruses was prepared and carried out using two different ways of viral RNAs extraction. Based on the results, it could be reported that viral RNAs for cDNA amplification by multiplex RT-PCR could be extracted from a mixture of infected leaves or separate extraction of each viruses infected leaves. In addition, results presented in this study demonstrated the application of multiplex RT-PCR to simultaneously detect CGMMV and KGMMV from cucurbit leaves using a mixture of four primers and its feasibility as a sensitive and rapid laboratory assay. Since, no multiplex RT-PCR technique has been described for the detection of CGMMV and KGMMV, this technique can be a good option for sensitive and reliable tool for detection of two major cucurbit infecting Tobamoviruses.Keywords : Cucurbit infecting Tobamovirus, multiplex RT-PCR, seed borne viruses
Genetic Variation Analysis of Mold (Magnaporthe oryzae B.Couch) Using Random Amplified Polymorphic DNA Pramono, Ajeng Kusumaningtyas; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Magnaporthe oryzae B.Couch is a host-specific fungi, certain strain only infect certain host plant species. Genetic variety among M. oryzae isolates was explained by dendogram which was constructed using similarity data of Random Amplified Polymorphic DNA (RAPD). Dendogram construction was achieved by computer software, Numerical Taxonomy System (NTSYS). The aim of the research were to study the genetic variation among M. Oryzae using RAPD and to construct a dendogram of genetic similarities among the ten isolates from green foxtail (Setaria viridis L.), finger millet (Eleusine coracana L.) and rice (Oryza sativa L.).RAPD was performed in 30 cycles using 5 primers (OPA-02, OPA-03, OPA-04, OPA-05, OPA-07). Polymorphism data was used to constructed dendogram using Dice index and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) in NTSYS software. There were 68 polymorphism fragments from 74 amplified fragments.Three clusters were formed in the dendrogram, based on host pathotype: foxtail millet type, finger millet type and rice type. There were two subclusters in foxtail millet type based on mating type, MAT1-1 dan MAT1-2. Thus, RAPD could be used as a method for genetic variation analysis of Magnaporthe oryzae to show host-specific specificity.Key words: Magnaporthe oryzae, RAPD, mating type
Co-Authors Achmadi Priyatmojo Agus Hery Susanto Agus Nuryanto Ajeng Kusumaningtyas Pramono, Ajeng Kusumaningtyas Alin Liana, Alin Anak Agung Gde Raka Swastika, Anak Agung Gde Raka Arfa, Namira Nur Ariestya, Ganies Riza Bani, Polikarpia Wilhelmina DIAN ARUNI KUMALAWATI E. Suharyanto, E. Endang Semiarti Faizatul Fitriyah, Faizatul Fitri Nadifah Friska, Meiliana Galuh Tresnani Ganies Riza Aristya, Ganies Riza Ganies Rizaa Aristya, Ganies Rizaa I Wayan Suardana Ignatius Julijantono, Ignatius Indraningsih, Estiyani Irma Nofitahesti, Irma Issirep Sumardi Iwan Roosdianto Jang, Soenghoe Joko Prastowo KEIKO T. NATSUAKI Keiko T. Natsuaki, Keiko T. Keiko T. Natsuaki`, Keiko T. KENJI WAKUI Lawrie, Muhammad Dylan Linda Oktavianingsih, Linda Mahfut Mahfut, Mahfut Maryani Maryani Miftahul Mushlih, Miftahul Muhammad Taufiq Qurrohman, Muhammad Taufiq Nofriarno, Nugroho Nofriarno, Nugroho Parazulfa, Anisa Prabawani, Ratri Lila Purnomo . Purnomo Purnomo Puspita, Utin Elsya RABBANI, ANNAS REFLINUR REFLINUR Rejeki Siti Ferniah Ridesti Rindyastuti Rina Sri Kasiamdari Rini Etika Ranis, Rini Etika Rizko Hadi, Rizko Rugayah Rugayah Saputri, Avia Purnama Saragih, Hendry Tri Sakti Sedyo Hartono Sigit Dwi Maryanto Siti Nur Azizah Fauziati Rahma, Siti Nur Azizah Fauziati Sri Wahyuningsih Sudarsono . Sukarti Moeljopawiro Susamto Somowiyarjo Tri Joko Tuty Arisuryanti, Tuty Wayan Tunas Artama Widya Asmara Wijaya, I Made Saka Wisnu Nurcahyo Y. Andi Trisyono Yambise, Hani Christin Yasir Sidiq Yuanita Rachmawati, Yuanita