Latifah K. Darusman
Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Jl. Meranti, Kampus IPB Dramaga, Bogor, 16680, Indonesia Pusat Studi Biofarmaka, Institut Pertanian Bogor, Jl. Taman Kencana No. 3, Bogor, Indonesia

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Variasi Bahan Bioaktif dan Bioaktivitas Tiga Nomor Harapan Temulawak pada Lokasi Budidaya Berbeda Nurcholis, Waras; Purwakusumah, Edy Djauhari; Rahardjo, Mono; Darusman, Latifah K.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Temulawak (Curcuma  xanthorrhizaRoxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicines. The research was aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity), and to obtain information on agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro antioxidant and toxicity were determined by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that promising line A produced the highest yield of bioactive and bioactivity, i.e. 0.157 and 0.056 g plant-1of xanthorrizol and curcuminoid respectively. The IC50 of antioxidant activity was 65.09 mg L-1and LC50of toxicity was 69.05 mg L-1. In this study, Cipenjo had the best temulawak performance than two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 ºC, rainfall ± 223.97 mm year-1 and sandy clay soil. Keywords: antioxidant, curcuminoid, promising lines, temulawak, xanthorrhizol
Extract of Curcuminoid Temumanangga (Curcuma mangga) in Inhibition Oxidation Reaction of Low Density Lipoprotein by Macrophage Susmiat, Trini i; ., Sulistiyani; Sajuthi, Dondin; Darusman, Latifah K.
Forum Pasca Sarjana Vol 33, No 1 (2010): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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The oxidation of low-density lipoprotein (LDL) is believed to be the initiating factor for the development and progression of atherosclerosis.  Curcuminoid, the metabolite of Zingiberaceae family such as temu mangga (Curcuma mangga), has been shown to reduce the susceptibility of LDL to oxidation. In this study, we examined the effect of curcuminoid extracted from temu mangga on copper ion-induced lipid peroxidation of low density lipoprotein (LDL) in mice’s macrophages and Macaca nemestrina’s monocytes.  Analyses were done by measuring the formation of thiobarbituric acid reactive substance (TBARS) as malonaldehyde (MDA).  LDL were harvested and isolated from 5 adult female Macaca fascicularis  fed aterogenic diet for 3 months.  LDL oxidation by mice macrophage incubated for 4 hours were inhibited by curcuminoid at concentration of 8 ppm.  There was decreased 17% (P
The Effectiveness of High Speed Aeration to Reduce Pollutant from Final Waste Disposal Leachate: Case Study at Galuga Final Waste Disposal Site (TPA) Bogor City ., Nurhasanah; Darusman, Latifah K.; Sutjahjo, Surjono Hadi; Lay, Bibiana Widiati
Forum Pasca Sarjana Vol 34, No 1 (2011): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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A laboratory scale research to obtain environmentally safe effluent from Final Waste Disposal Site leachate in Galuga owned by Regional Government of Bogor City was conducted from July 2006 through to April 2007. The experiment of production tolerable threshold effluent was carried out by aerating the leachate in 4 different aeration rates (0, 10, 30 and 70 liters/minute).  The research found that the most effective method to reduce pollutant was aerating at the rate of 70 liters/minutes.  However, only some of pollutant (Cu, Zn, Pb and E.coli) can be reduced until below tolerable threshold.   Keywords: effluent, leachate, tolerable threshold
Respon Ekspresi Molekul Adhesi pada Kultur Sel Endotel yang diinduksi LDL Teroksidasi dari Macaca fascicularis terhadap Ekstrak Kurkuminoid Temumangga Susmiati, Trini; ., Sulistiyani; Sajuthi, Dondin; Darusman, Latifah K.
Jurnal Primatologi Indonesia Vol 6, No 2 (2009)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Abstract

Curcuminoids of Curcuma manggo believed to have antioxidant properties that can prevent the occurrence of atherosclerosis.  However, the mechanism at the molecular level is not clearly known. Increase in intracellular adhesion molecule-1 (ICAM-1) will result in endothelial cell dysfunction that initiates athersclerosis. The expression of ICAM-1 can be triggered by oxidized LDL molecules. The purpose of this study is to elucidate the effects of curcuminoids temumangga (Curcuma mango) on the expression of ICAM-1 induction in endothelial cell culture. Prior to the induction of the expression of ICAM-1, endothelial cell culture was pre-incubated with curcuminoids extract for 48 hours with concentration of 2 ppm and 8 ppm, then further incubated with oxidized LDL for 24 hours. The treated cells were compared to 5 µM Cu2+ induced endothelial cell culture with or without LDL  from Macaca fascicularis.  ICAM-1 expression was determined by immunohistochemistry using anti-ICAM-1 antibodies and visualized with staining by 3.3-diaminobenzidin tetrahidrochloride (DAB). Results showed that surface-induced endothelial cell culture with ion Cu2+ stained well by DAB as brown yellow, which indicates ICAM-1 expression. Curcuminoids extract of  8 ppm reduced induction of ICAM-1 response. Induction of ICAM-1 expression by oxidized LDL caused foam cell accumulation in addition to ICAM-1 expression.Key words: Curcuminoid, low density lipoprotein (LDL), Macaca fascicularis, intracellular adhesion molecule-1 (ICAM-1), immunohistochemistry
MODEL OTENTIKASI KOMPOSISI OBAT BAHAN ALAM BERDASARKAN SPEKTRA INFRAMERAH DAN KOMPONEN UTAMA STUDI KASUS : OBAT BAHAN ALAM/FITOFARMAKA PENURUN TEKANAN DARAH Soleh, Agus Mohamad; Darusman, Latifah K.; Rafi, Mohamad
FORUM STATISTIKA DAN KOMPUTASI Vol 13, No 1 (2008)
Publisher : FORUM STATISTIKA DAN KOMPUTASI

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Abstract

Komposisi kimia yang terkandung dalam ekstrak obat bahan alam merupakan suatu komposisi yang kompleks, dengan demikian pengujian keotentikannya tidak dapat dilakukan melalui pedekatan tunggal.  Salah satu teknik analisis yang dapat menggambarkan secara menyeluruh karakteristik kimia suatu bahan adalah teknik spektroskopi FTIR. Spektra FTIR dihasilkan dari interaksi antara energi sinar inframerah dan komponen kimia penyusun campuran bahan, sehingga suatu spektra FTIR merupakan indentitas khas campuran tersebut. Keotentikan komposisi suatu obat bahan alam pada studi  ini ditentukan berdasarkan pada analisis komponen utama spektra inframerahnya.  Studi dilakukan pada obat bahan alam/fitofarmaka penurun tekanan darah (Tensigard® : terdiri dari ekstrak seledri dan ekstrak daun kumis kucing). Pengukuran spektra inframerah dilakukan terhadap formula obat yang persentase komposisinya ditentukan melalui simplex lattice design. Selain itu pengukuran spektra inframerah juga dilakukan terhadap formula obat dengan mengganti (adulterasi) ekstrak kumis kucing dengan obat sintetis (reserpin) dan ekstrak sambiloto. Berdasarkan plot antara skor komponen utama pertama dan skor komponen utama kedua menunjukkan plot tersebut dapat digunakan untuk mendeteksi komposisi obat, tetapi tidak dapat mendeteksi adanya adulterasi komposisi oleh bahan lain.   Kata Kunci : model otentikasi fitofarmaka, simplex lattice design, komponen utama, tensigard
SUPLEMENTASI STEROL LEMBAGA GANDUM (Triticum sp.) PADA MARGARIN Marliyati, Sri Anna; Syarief, Hidayat; Muchtadi, Deddy; Darusman, Latifah K.; ., Rimbawan
Jurnal Teknologi Dan Industri Pangan Vol 21, No 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

SUPLEMENTASI STEROL LEMBAGA GANDUM (Triticum sp.) PADA MARGARIN (Supplementation of Margarine with Wheat Germ Sterol) Sri Anna Marliyati1)*, Hidayat Syarief1), Deddy Muchtadi2), Latifah K. Darusman3), dan Rimbawan1) 1) Departemen Gizi Masyarakat, FEMA IPB 2) Departemen Ilmu dan Teknologi Pangan, Fateta IPB 3) Departemen Kimia, F-MIPA IPB   Diterima  25 November 2009 / Disetujui 26 April  2010 ABSTRACT   Margarine is a water in oil (w/o) emulsion product which is widely used for household cooking and baking industry. Consuming of margarine, which contains trans fatty acid may cause health problem due to the increase of LDL cholesterol. Since margarine is also a good carrier of phytosterol which prevent the absorption of cholesterol, there is a possibility to formulate a healthier margarine. In this research formulation and characteristics of products was investigated. The research work consisted of two steps: (1) supplementation of wheat germ sterol into margarine (two methods) and  (2) analysis of physical, chemical characteristics and hedonic score. Parameters of physical characteristics were melting point and emulsion stability, whereas chemical characteristics were water and oil contents. The hedonic test was carried out based on product’s color, odor, taste, texture, and spreadability. Results showed that method II of supplementation produced better margarine than method I, in which the concentration of sterol in the margarine was higher with a melting point similar to that of control, better emulsion stability, and higher hedonic score. Supplementation process was carried out by mixing sterol into fat phase melted at 50 0C, followed by mixing with aqueous phase at 4 0C. Sterol used for method II was extracted using mixed solvent of hexane and ethanol at the ratio of 1:2 (v/v), which was resulted from previous experimentation.   Key words: supplementation, wheat germ sterol, margarine
Studies on Dormancy Periods and Growth Rhythm of Shoot and Root of Mangosteen (Garcinia mangostana L.) Hidayat, Ramdan; Surkati, Achmad; Poerwanto, Roedhy; Darusman, Latifah K.; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 33, No 2 (2005): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Mangosteen has a good prospectus for international market. Many people in the world like this fruit because it is delicious, has high nutrient contents, and it can be consumed as a fresh fruit. The growth of mangosteen is very slow with a very long juvenile period because of its long dormancy period. The objective of this research was to study the dormancy periods and growth rhytm of shoot and root of young and adult mangosteen seedlings. The research used completely randomized design and consisted of two experiments. The first experiment was to study the dormancy and flushing periods. The second experiment was to study the growth patterns of shoot and root foom several ages of mangosteen seedlings. This research was conducted at Mekarsari Fruit Park, Cileungsi, Bogor. The result of the experiment showed that two years old of mangosteen seedling had 5 flushes per year, four years old of mangosteen had 3-4 flushes per year, and eight years old of mangosteen only had 2 flushes. Dormancy periods were significantly different between 2,4 and 8 year of mangosteen seedling. After the seedlings have branches, the dormancy periods become twice as many as that before branching. The roots grew rapidly two weeks before flush and slower after flush occurred.   Key words: Garcinia mangostana, dormancy, flush, seedling
Pengaturan Pembungaan Tanaman Manggis (Garcinia mangostana L.) di Luar Musim dengan Strangulasi, serta Aplikasi Paklobutrazol dan Etepon Rai, I Nyoman; Poerwanto, Roedy; Darusman, Latifah K.; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 32, No 2 (2004): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

The objective of the research was to study the application of strangulation, packlobutrazol and ethephon to stimulate flowering of mangosteen trees. The experiment was conducted from June 2002 to September2 003, located at Farmer´s Mangosteen Orchards, Leuwiliang, Bogor. A randomized block design with two factors was used in thisexperiment. The first factor was treatments to stimulate flowering consisted of three levels i.e strangulation (8), (S) application of paclobubtrazol (P) and control (K). The second factor was treatments for dormancy breaking consisted of two levels i.e without ethephon (Eo) and with ethephon (EiJ. Each treatment was replicated four times. The resultshowed that strangulation and application of paclobutrazol were effective in stimulating flowering of mangosteen trees. Those treatments decreased gibberellic acid content and increased total sugar and C: N ratio. Interaction effect was found between treatments to stimulate flowering and ethephon on number of flowers, number of fruits and weight of fruits per tree. The highest fruits weight (2.,50 kg/tree) was obtained on the combination of paclobutrazol with ethephon while the lowest was in control without ethephon( 6.05 kg/tree).Key words: Mangosteen, Strangulation, Paclobutrazol, Ethephon, Flowering;
Pertumbuhan, Produksi dan Kandungan Triterpenoid Dua Jenis Pegagan (Centella asiatica L. (Urban)) Sebagai Bahan Obat pada Berbagai Tingkat Naungan Kurniawati, Ani; Darusman, Latifah K.; Rachmawaty, Rani Yulie
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 33, No 3 (2005): Jurnal Agronomi Iindonesia
Publisher : Bogor Agricultural University

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Abstract

The research was aimed to determine the optimum shading level and effect two types of Indian Pennywort (Centella asiatica L. Urban)) on growth, production, and triterpernoid. The research was conducted on  March until November 2004 at BIOFARMAKA Research Station, Darmaga and chemical analysis was conducted at Analytical Chemistry Laboratory, FMIPA, Baranangsiang. Split plot design with two factors was used in this experiment. The first factor was shading level as main plots consisting of 0, 25, 55, and 75% shading level. The second factor was type of Indian Pennywort  as subplots  consisting  of big and small Indian Pennywort. Result of the reseach showed that shading effects decrease leaf area indeks, yield, and composition of triterpenoid content of Indian Pennywort. No shading treatment  showed optimum growth and yield. The combination of 25% shading and small type of Indian Pennywort  gave the highest triterpenoid content. Both types of Indian Pennywort showed best growth; the difference in length of petiole, number of leaf  and shoots but the small one gave the best yield.   Key words :  Indian Pennywort type, shading level, triterpenoid  
UJI IN VITRO EKSTRAK AIR DAN ETANOL DARI BUAH ASAM GELUGUR, RIMPANG LENGKUAS, DAN KENCUR SEBAGAI INHIBITOR AKTIVITAS LIPASE PANKREAS Iswantini, Dyah; Darusman, Latifah K.; Fitriyani, Ana
Jurnal Sciene dan Teknologi Vol 12, No 1 (2010): JURNAL SAINS DAN TEKNOLOGI INDONESIA
Publisher : Jurnal Sciene dan Teknologi

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Abstract

Asam gelugur fruits of Garcinia often used to reduce body weight. Lengkuas and kencur are traditional herbal that potential for antiobesity because they could reduce the level of phospholipids, triglycerides, and cholesterol. The aim of the research was to evaluate the potencies of these herbal as antiobesity by measurement of their water and ethanol extracts capabilities as in vitro inhibitor of pancreatic lipase activity. The water and ethanol extracts of asam gelugur fruits contained saponins and alkaliods, respectively. The water extract of lengkuas rhizomes contained alkaloids, flavonoids, saponins, and quinones, while the ethanol extract contained alkaloids, flavonoids, saponins, and steroids. The water extract of kencur rhizomes contained saponins and quinones, while the ethanolextract contained alkaloids, flavonoids, steroids, and quinones. The highest inhibitory effect of all extracts was obtained from the ethanol extract of asam gelugur fruits with value of 86.3% at 150 ppm. The highest inhibitory effect of lengkuas extracts was from the ethanol extract at 200 ppm (56.2%). The highestinhibitory effect of kencur was showed by the ethanol extract with the value 37.6% at 300 ppm. These values were higher than the inhibitory effect of the positive control (Xenical®) at 100 ppm (10.6%).