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Journal : Jurnal Medika Veterinaria

ISOLASI BAKTERI Salmonella sp. PADA FESES ANAK AYAM BROILER DI PASAR ULEE KARENG BANDA ACEH (Isolation of Salmonella sp. in Feces of Broiler Chicks at Ulee Kareng Market Banda Aceh) Afriyani, Afriyani; Darmawi, Darmawi; Fakhrurrazi, Fakhrurrazi; Heryawati Manaf, Zakiah; Abrar, Mahdi; Winaruddin, Winaruddin
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.039 KB) | DOI: 10.21157/j.med.vet..v10i1.4047

Abstract

The research aimed to isolate Salmonella sp.in feces of broiler chicks. Fifteen feces samples of broiler chick were obtained from Ulee Kareng Market in Banda Aceh. Chick cloaca was swabbed using a sterile cotton swab, put into the microtube, and brought to the Microbiology Laboratory of Veterinary Medicine Faculty, Syiah Kuala University. The samples were incubated in selenite cystine broth (SCB) medium, cultured in salmonella shigella agar (SSA) and separated colonies were inoculated in nutrien agar (NA) at 37° C for 24 hours. Bacteria stained with Gram staining then tested biochemically. The result showed that Salmonella sp. were identified in 9 out of 15 feces chicks samples. It ca be concluded that broiler chick in Ulee Kareng Market Banda Aceh contaminated with bacteria Salmonella sp.
IDENTIFIKASI BAKTERI Staphylococcus aureus PADA PREPUTIUM DAN VAGINA KUDA (Equus caballus) (Identification of Staphylococcus aureus in Preputium and Vagina of Horses (Equus caballus)) Rahmi, Yulina; Darmawi, Darmawi; Abrar, Mahdi; Jamin, Faisal; Fakhrurrazi, Fakhrurrazi; Fahrimal, Yudha
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.354 KB) | DOI: 10.21157/j.med.vet..v9i2.3805

Abstract

This research aims to identify the Staphylococcus aureus (S. aureus) bacteria from preputium and vagina of horses. Five swabs preputium and 5 swabs vagina samples from 3-9 years olds horse in the village of Bebangka, Gelelungi, Reloop, and Kelapeng, Pegasing Aceh Tengah District were used in this study. Preputium and vagina swabs of horses were dipped into reaction tube containing Nutrient Broth (NB). Staphylococcus aureus was identified on manitol salt agar (MSA). Separated colony colored bright yellow on the media were tested for Gram staining, hemolysis, catalase, and sugar test (glucose and manitol). The result showed that the isolates growth on MSA were colored bright yellow, Gram positive, β hemolisis, and catalase positive. The isolates fermented manitol and glucose. Isolates identified from horses preputium and vagina showed the characteristic of S. aureus. In conclusion S. aureus bacteria were positively identified from all of swabs samples, both in preputium and vagina of horses.Key words: Staphylococcus aureus, horse, preputium, vagina
ISOLASI BAKTERI COCCUS GRAM POSITIF DI DALAM SUSU ULTRA HIGH TEMPERATURE (UHT) 6 DAN 3 BULAN MENJELANG KEDALUWARSA (Isolation of Gram-Positive Cocci Bacteria in Ultra High Temperature (UHT) Processed Milk at 6 and 3 Months Prior to Expiration) Sahputra, Dedy; Ferasyi, T. Reza; Ismail, Ismail; Razali, Razali; Sulasmi, Sulasmi; Darmawi, Darmawi
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.025 KB) | DOI: 10.21157/j.med.vet..v10i1.4038

Abstract

This research was aimed to isolate the Gram-positive bacteria that contaminated milk processed with ultra high temperature (UHT) at 6 and 3 months before the date expires. A number of 3 different UHT milk products at 6 and 3 months before expire date were used as sample. Milk samples were taken from several supermarket in Banda Aceh. The identification was conducted using Gram staining method. One ml milk sample was transferred into 18 tubes containing nutrient broth (NB) media and incubated at 37 C for 24 hrs. The sample was then culture on 18 petri discs containing manitol salt agar (MSA) and 18 petri discs containing trypticase soy sucrose bacitracin (TYS20B) using streak plate method. After 2 days, bacterial colonies that grow were identified by Gram staining. The results showed that in UHT processed milk at 6 and 3 prior to expiration contaminated with Gram positive cocci bacteria, which comprised of Staphylococcus aureus and Streptococcus mutant. As conclusion, UHT processed milk at 6 and 3 prior to expiration is contaminated by Gram positive cocci bacteria.
KONTAMINASI BAKTERI KOLIFORM PADA AIR MINUM ISI ULANG DI DESA ILIE KECAMATAN ULEE KARENG KOTA BANDA ACEH (Contamination of Coliform in Refill Water in Ilie Village, Ulee Kareng, Banda Aceh) Pratama Sekedang, M. Iqbal; Heryawati Manaf, Zakiah; Darmawi, Darmawi; Jamin, Faisal; Abrar, Mahdi; Razali, Razali
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.513 KB) | DOI: 10.21157/j.med.vet..v10i1.4046

Abstract

The purpose of this study was to find out whether the refill water (AMIU) was contaminated with coliform or not. The methods used were presumptive, confirmed, and completed test. Sample of this study was refill water (AMIU) from five refill water shops located in Ilie village, sub-district of Ulee Kareng, Banda Aceh. The sample was taken 3 times with interval period of one week. Sample was culture in lactose broth and incubated at 37° C for 48 hours. If it did not form gas in Durham tube, presumptive test result was considered negative and the samples were not proceed to the other test. The data was presented using descriptive analysis. It could be concluded that AMIU from 5 shops in Ilie Ulee Kareng, Banda Aceh is negatively contaminated with coliform bacteria.
PENGARUH PENCELUPAN KARKAS AYAM PEDAGING DALAM LARUTAN ASAM SITRAT DAN ASAM ASETAT TERHADAP ANGKA LEMPENG TOTAL Escherichia coli (The Immersion Effect of Broiler Carcass in Citric Acid and Acetic Acid on the Total Plates Count of Escherichia Coli) Nurliana, Nurliana; Yuda, Sandy Cakra; Jamin, Faisal; Ferasyi, Teuku Reza; Isa, M.; Darmawi, Darmawi
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.496 KB) | DOI: 10.21157/j.med.vet..v9i2.3812

Abstract

This study aimed to determine the immersion effect of broiler carcasses in 3% citric acid, 3% acetic acid, and combination of citric acid and acetic acid with concentration of 1.5% each on the total plate count of bacteria E. coli. Eight broilers carcasses (dissected into 2 parts) were divided into 4 treatment groups with 2 times repetition and 3 times observation at 0, 4, and 8 hours after dipped. The control treatment (K0) without dipped into acid solution. Broiler carcasses in the first (K1), second (K2), and the third (K3) treatments were dipped into 3% acetic acid, 3% citric acid, and combination of 1.5% acetic acid and 1.5% citric acid respectively. Data were analyzed using analysis of variance. The average of E. coli count in K0 at 0, 4, and 8 hours post dipping were 5.2±0.061, 6.8±0.99, and 7.0±0.107 log 10 cfu/g of chicken meat respectively. In similar observation time with K0, the average of total E. coli count were 5.5±0.373, 6.0±0.023, and 6.0±0.242 log 10 cfu/g of chicken meat in K1, 5.3±0.166, 6.0±0.143, and 6.0±0.084 log 10 cfu/g of chicken meat in K2, 5.7±0.041, 5.9±0.92, and 5.8±0.058 log 10 cfu/g of chicken meat in K3 at 0,4, and 8 hours post dipping respectively. Statistical analysis showed that the immersion of broiler carcass in acetic acid and citric acid solution were significantly (P <0.05) decrease the number of E. coli. There were interaction between immersion of broiler carcass in organic acids and observation time which significantly decrease the number of E. coli (p <0.05). In conclusion, the immersion of carcass chicken in 3 % acetic acid, 3% citric acid, and their combination reduce the amount of E. coli. Key words: chicken carcass , citric acid 3%, acetic acid 3% , Escherichia coli
9. Total colony of cellulolitic bacteria in the rumen of aceh cattle Safika, Safika; Matondang, Siti Wardianah; Darmawi, Darmawi; Abral, Mahdi; Erina, Erina; Jalaluddin, M
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.784 KB) | DOI: 10.21157/j.med.vet..v11i1.3411

Abstract

The aim of this study was to count the total sum of cellulolitic bacteria colony in  aceh cattle rumen. The sample used was aceh cattle rumen liquid which taken from salughtered house in Banda Aceh. Aceh cattle rumen liquid were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Petri dish was entered to candle jar to be incubated in an incubator with temperature 37oC about 96 hours. Colony of cellulolitic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of colony), counting total of bacteria which was grown, and Gram staining. The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil. The average number bacteria of total bacteria in the cattle rumen liquid was 4,81 x 105 cfu/ml
5. Isolation and Identification of Salmonella sp in spleen of male layer chicken in Sibreh farms, Aceh Besar Erina, Erina; Abrar, Mahdi; Suyoto, Balqis Alrasyid; Dewi, Maryulia; Darmawi, Darmawi; Darniati, Darniati; Bakri, Muttaqien
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.108 KB) | DOI: 10.21157/j.med.vet..v11i1.3986

Abstract

This study aimed to isolate and identify the bacteria Salmonella sp in spleen of male layer chicken that died in farms Sibreh, Aceh Besar. A sample of the research is the spleen of male layer chickens. Isolation of Salmonella sp performed by inoculating spleen that were pierced using a swab on the media Selenite Cystine Broth (SCB) then planted on selective media Salmonella Shigella Agar (SSA) and colony morphology was observed, continued with observation of Gram stain under microscopic. Furthermore, colonies of suspected Salmonella sp planted in the media MR-VP (Oxoid), Indol (Difco), Simmonss Citrate Agar (Oxoid), Triple Sugar Iron Agar (TSIA), Sulfite Indol Motiliti (SIM), and media sugars (sucrose, manitol, glucose, lactose , maltose). The results of this study were analyzed descriptively. The research shows a results from a total of 33 samples, we found that 15 samples are positive that can be isolated and identified as Salmonella sp. Therefore it can be concluded that Salmonella sp is possible the death cause of male layer chicken in farms Sibreh, Aceh Besar
10. Isolation and Identification of Lactic Acid Bacteria Genus Pediococcus from Sumatran Orangutan (Pongo abelii) faeces at Kandi Zoo and Kinantan Zoo West Sumatera Reimena, Resti; Erina, Erina; Darniati, Darniati; Fakhrurrazi, Fakhrurrazi; Darmawi, Darmawi; Budiman, Hamdani
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.441 KB) | DOI: 10.21157/j.med.vet..v11i1.3524

Abstract

This research has purposes to isolating and identifying the lactic acid bacteria genus Pediococcus in sumatran orangutan (Pongo abelii) feaces. Feaces are isolated from one orangutan at Kandi Zoo and two orangutans at Kinantan Zoo. The isolating of the lactic acid bacteria was done by using streak plate method with the MRSA (de Man Rogosa Sharpe Agar) specific media. Identification was done by the morphological observing, Gram stain, catalase test, oxidase test and biochemical test. The result showed that one isolate feaces from Kandi Zoo has cocci shaped with the isolate prediction approaches genus Pediococcus sp. The characteristic features of isolate are cocci shaped, Gram positive, tetrad cell structure, nonmotile, catalase negative, and oxidase negative. Based on these characteristics, it can be concluded that there is a lactic acid bacteria coloni which grouped into genus Pediococcus in male orangutan feaces from Kandi Zoo
17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate Dewi, Maryulia; Darmawi, Darmawi; Helmi, T. Zahrial; Erina, Erina; Gani, Basri; Eliawardani, Eliawardani; Azhar, Azhar
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.621 KB) | DOI: 10.21157/j.med.vet..v13i1.8884

Abstract

The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline  had the ability to inhibit the biofilm of Staphylococcus aureus.
8. Histopathological of Fabricius Bursa in the Broiler Chicken after Vaccination with Newcastle Disease Virus Vaccine Balqis, Ummu; Annah, Nurjannah; Budiman, Hamdani; Darmawi, Darmawi; Iskandar, Cut Dahlia; Hasan, M.
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (988.122 KB) | DOI: 10.21157/j.med.vet..v13i1.4178

Abstract

The aim of this research was to determine an effect of Newcastle disease virus (NDV) vaccine to histopathologic on Fabricius bursa of broiler chicken at various age levels. Eighteen commercial DOC (Day Old Chick) were divided into 2 groups namely a control group that was given distilled water and a treatment group that was given NDV vaccine namely ND Lasota vaccine ocularly as much one drop per eye at day 3, then a booster dose in drinking water at day 13 was given. The bursa of Fabricius was collected from these chickens on the age of 14 days, 21 days, and 28 days after sacrificing the chickens through necropsied, then histopathological preparations are made using hematoxylin-eosin staining. The parameters observed were measured the number, diameter, and thickness of cortical of lymphoid follicles. Data were analyzed descriptively and quantitatively by unpaired t-test. The results of this research showed the NDV vaccine effects were not significantly different (P>0.05) to the number and diameter of lymphoid follicles. In the treatment group, 14 days old broiler chickens had a decreased number of lymphoid follicles, while its diameter tends to grow and the thickness of cortex was also seemed unchanged compared with the control group. Then at the age of 21 days, the number, diameter, and thickness of cortex of lymphoid follicles tend to increase, while at 28 days diameter of lymphoid follicles tend to be slightly reduced, but the number and thickness tend to grow out of control. It was concluded that the NDV vaccine had a good effect on the Fabricius bursa of broiler chickens with increasing age, then histopathological preparations are made using hematoxylin-eosin staining.
Co-Authors Abdul Harris Abdullah Hamzah, Abdullah Abral, Mahdi Admi, Masda Afriyani, Afriyani Ahmad, Sagasias Al Azhar Amalia Sutriana Ananda Nasution, Annisah putri Annah, Nurjannah Athaillah, Farida Ayuti, Siti Rani Azhar Azhar Azhari Azhari Basri Gani budianto panjaitan Cut Dahlia Iskandar Dani Rosdiana Darniati Darniati Daud AK, M. dwita, rina Eliawardani Eliawardani, Eliawardani Erdiansyah Rahmi Erina Erina Faisal Jamin Fakhrurrarzi, Fakhrurrazi Fakhrurrazi Fakhrurrazi Fema Amzani Nasution, Feby Fifia Chandra Fitriani Fitriani Ginting, Septian Tri Mulyana Hamdani Budiman Hamny Hamny Hasanuddin Hasanuddin Helmi, T. Zahrial Henni Vanda HENNIVANDA, HENNIVANDA Huriatul Masdar Husna Husna Ismail Ismail Jalaluddin, M M. Hasan M. Isa M. Nur Salim Mahdi Abrar Maryam Maryam Maryulia Dewi Matondang, Siti Wardianah Moliwati, Moliwati Momon Sodik Imanuddin Muhammad Hambal Muhammad Jalaluddin, Muhammad Mulyadi Adam, Mulyadi Mulyadi Mulyadi Muslina Muslina, Muslina Muttaqien Bakri, Muttaqien Muttaqien Muttaqien, Muttaqien Nasution, Borgo Mauly Nurdin, Hendri Nurhaspika, Nurhaspika NURLIANA NURLIANA Pratama Sekedang, M. Iqbal Ramadhani, Fitria Sari Rastina, Rastina Rastina, Rastina Razali Daud Razali Razali Reimena, Resti Reynaldi, Fitrah Reynaldi, Fitrah Riman Sipahutar Rinidar Rinidar, Rinidar Risa Tiuria Rosmaidar Rosmaidar, Rosmaidar Safika, Safika Safika, Safika Safika, Safika Safrizal Safrizal Sahputra, Dedy Samadi Samadi Saputri, Pragita Ayu Siti Aisyah Siti Hajar Siti Masreah Bernas Sugito Sugito Sulasmi Sulasmi Suyoto, Balqis Alrasyid Syafruddin Syafruddin Syahputra, Yandi T. Reza Ferasyi, T. Reza Teuku Reza Ferasyi Teuku Zahrial Helmi Teungku Nih Farisni, Teungku Nih Ummu Balqis Vara Tassa Sutari, Vara Tassa Veni Nella Syahputri, Veni Nella Waskito Waskito, Waskito Wenny Novita Sari Winaruddin Winaruddin xarolin, laras berlian Yarmaliza, Yarmaliza Yarmaliza, Yarmaliza Yuda, Sandy Cakra Yudha Fahrimal Yulina Rahmi, Yulina Yurliasni Yurliasni Zahra, Amalia Fatma Zakiah Heryawati Manaf, Zakiah Zakiyah Heryawati Manaf Zakiyuddin, Zakiyuddin Zulfan, Muhammad Zuraidawati Zuraidawati, Zuraidawati