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Journal : Proceedings of Annual International Conference Syiah Kuala University - Life Sciences

Mucosal Mast Cells Contribution in Intestinal Defense of Chickens (Gallus domesticus) Infected Naturally by Ascaridia galli Balqis, Ummu; Admi, Masda; Darmawi, Darmawi; Hambal, Muhammad
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This study was aimed at finding out the investigation of mucosal mast cells   in intestines of chicken  that  were  naturally  infected  by  Ascaridia galli.  Amount  of  ten  intestine  of  freshly slaughtered chickens (Gallus domesticus) found from local abatoir in Banda Aceh were divided into two groups containing five intestines of each. Mucosal mast cells count were done of which histologic slides were made in stained serial histological sections with Alcian blue (pH 0,3) and Safranin-O (pH 0,1) of the intestines. The result showed that the mucosal mast cells increased significantly (P < 0.05) in intestines of chickens infected naturally by survival A. galli adult worms. It was concluded that the intestinal defense of chickens against parasite infection is associated with the mucosal mast cells contribution by creating an environment hostile to the establishment and survival of intestinal nematodes, A. galli.
Histamine Levels in Repeated Thawing Beef Nurliana, Nurliana; Nasution, Borgo Mauly; Darmawi, Darmawi; Sugito, Sugito
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.256 KB)

Abstract

The aim of this research was to determine the histamine levels in repeated thawing beef. This research were using longisimus dorsi part (sirloin) of beef. Repeated thawing treatments of beef consisted of P1  as control without freezing, P2  as first thawing  after one week freezing, P3  as second thawing without freezing two weeks and P4 with freezing three weeks. The slices of beef was storage in freezer at temperature of -20ºC every weeks for three weeks. The thawing process was done by storage in refrigerator at temperature of 6ºC for 270 minutes after being removed from the storage in freezer every weeks for three weeks. PIC (Protease Inhibiting Cocktail) methode were used to extraction of enzyme in beef. Enzyme Link Imunosorbent Assay (ELISA) were used to detected the histamine levels with 450 nm wave length. The quantitative data from parameters were analyzed by descriptive. The results of the study concluded that repeated thawing beef in refrigerator at temperature of 6ºC could decrease the histamine levels. 
Inhibition of Salmonella sp. Growth in Comparison with Antibiotics and Pineapple (Ananas comosus) Squeeze Admi, Masda; Erina, Erina; Azhari, Azhari; Balqis, Ummu; Hambal, Muhammad; Darmawi, Darmawi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1063.056 KB)

Abstract

The purpouse of this study to examine antibiotics inhibition growth of Salmonella sp. isolated from feces of broiler chickens to chloramphenicol, gentamicin, and tetracycline in coparison with pineapple squeeze. Swab  sample  from  broiler  chicken  cloaca  was  cultured  to  nutrient  broth  media  and incubated at 37°C temperature for 24 hours. Culture was compared with Mc Farland 3 standardization, and separated on surface of Muller Hinton Agar (MHA). Antibiotic sensitivity was tested by mean of  chloramphenicol, gentamisin, and tetrasiklin in coparison with pineapple squeeze. The antibiotic disks (Oxoid) were put on the surface of MHA media which had inoculated with Salmonella sp. bacteria. Antibiotic sensitivity was determined based on formation of inhibition zone in surrounding of antibiotic disc. The result showed that Salmonella sp. bacteria growth was inhibited in ≥ 21 mm and ≥ 18 mm, ≥ 7 mm and ≥ 9 mm against chloramphenicol, gentamisin, tetrasiklin, and pineapple squeeze respectively. This research concluded that the Salmonella sp. cultured in this manner were sensitive to chloramphenicol and gentamisin. The inhibition growth of Salmonella sp. caused by chloramphenicol and gentamicin was significantly higher in comparison with pineapple (Ananas comosus) squeeze, meanwhile the Salmonella sp. bacteria was resistance against tetracycline. Our result suggest that both antibiotics chloramphenicol and gentamicin were recommended for antibiotic therapy in Salmonella sp. infection.
Co-Authors Abdul Harris Abdullah Hamzah, Abdullah Abral, Mahdi Admi, Masda Afriyani, Afriyani Ahmad, Sagasias Al Azhar Amalia Sutriana Ananda Nasution, Annisah putri Annah, Nurjannah Athaillah, Farida Ayuti, Siti Rani Azhar Azhar Azhari Azhari Basri Gani budianto panjaitan Cut Dahlia Iskandar Dani Rosdiana Darniati Darniati Daud AK, M. dwita, rina Eliawardani Eliawardani, Eliawardani Erdiansyah Rahmi Erina Erina Faisal Jamin Fakhrurrarzi, Fakhrurrazi Fakhrurrazi Fakhrurrazi Fema Amzani Nasution, Feby Fifia Chandra Fitriani Fitriani Ginting, Septian Tri Mulyana Hamdani Budiman Hamny Hamny Hasanuddin Hasanuddin Helmi, T. Zahrial Henni Vanda HENNIVANDA, HENNIVANDA Huriatul Masdar Husna Husna Ismail Ismail Jalaluddin, M M. Hasan M. Isa M. Nur Salim Mahdi Abrar Maryam Maryam Maryulia Dewi Matondang, Siti Wardianah Moliwati, Moliwati Momon Sodik Imanuddin Muhammad Hambal Muhammad Jalaluddin, Muhammad Mulyadi Adam, Mulyadi Mulyadi Mulyadi Muslina Muslina, Muslina Muttaqien Bakri, Muttaqien Muttaqien Muttaqien, Muttaqien Nasution, Borgo Mauly Nurdin, Hendri Nurhaspika, Nurhaspika NURLIANA NURLIANA Pratama Sekedang, M. Iqbal Ramadhani, Fitria Sari Rastina, Rastina Rastina, Rastina Razali Daud Razali Razali Reimena, Resti Reynaldi, Fitrah Reynaldi, Fitrah Riman Sipahutar Rinidar Rinidar, Rinidar Risa Tiuria Rosmaidar Rosmaidar, Rosmaidar Safika, Safika Safika, Safika Safika, Safika Safrizal Safrizal Sahputra, Dedy Samadi Samadi Saputri, Pragita Ayu Siti Aisyah Siti Hajar Siti Masreah Bernas Sugito Sugito Sulasmi Sulasmi Suyoto, Balqis Alrasyid Syafruddin Syafruddin Syahputra, Yandi T. Reza Ferasyi, T. Reza Teuku Reza Ferasyi Teuku Zahrial Helmi Teungku Nih Farisni, Teungku Nih Ummu Balqis Vara Tassa Sutari, Vara Tassa Veni Nella Syahputri, Veni Nella Waskito Waskito, Waskito Wenny Novita Sari Winaruddin Winaruddin xarolin, laras berlian Yarmaliza, Yarmaliza Yarmaliza, Yarmaliza Yuda, Sandy Cakra Yudha Fahrimal Yulina Rahmi, Yulina Yurliasni Yurliasni Zahra, Amalia Fatma Zakiah Heryawati Manaf, Zakiah Zakiyah Heryawati Manaf Zakiyuddin, Zakiyuddin Zulfan, Muhammad Zuraidawati Zuraidawati, Zuraidawati