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Journal : Jurnal Kedokteran Hewan

TOTAL BACTERIA AND IDENTIFICATION OF PROTEOLYTIC RUMINAL AND RETICULUM BACTERIA FROM LOCAL KACANG GOAT Safika, Safika; Darmawi, Darmawi; Ramadhani, Fitria Sari; Nurhaspika, Nurhaspika; Moliwati, Moliwati
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.003 KB) | DOI: 10.21157/j.ked.hewan.v12i1.9288

Abstract

This study aimed to calculate the total bacteria and to identify the proteolytic bacteria in rumen and reticulum of local Kacang goat. The samples used were the rumen and reticulum fluids of five goats at abattoirs in Banda Aceh. Isolation of proteolytic bacteria was carried out using skim milk agar with pour plate method and incubated at 39° C for 48 hours. The bacterial colonies morphology was observed and the total bacterial count was recorded. DNA of the widest proteolytic index colony was isolated, amplified, and sequenced. The results showed that the dominant colonies morphology was white with position inside the agar. The average of total proteolytic bacteria in goat rumen fluid and reticulum fluid were 6.416x106 CFU/mL and 2,382x107 CFU/mL, respectively. Isolates with the widest proteolytic index was Ru3 (2.5 mm) in the rumen which homology and phylogenetic tree analysis of 16S rRNA showed 93% sequence similarity with Bacillus subtilis, while in reticulum was Re1 (2.0 mm) which has 92% sequence similarity to Tatumella. It is concluded that the number of proteolytic bacteria in reticulum is greater than the number of proteolytic bacteria in the rumen of local kacang goat. Homology analysis in this study proved that the Ru3 and Re1 isolate were probably either a new species or unconfirmed species.
Co-Authors Abdul Harris Abdullah Hamzah, Abdullah Abral, Mahdi Admi, Masda Afriyani, Afriyani Ahmad, Sagasias Al Azhar Amalia Sutriana Ananda Nasution, Annisah putri Annah, Nurjannah Athaillah, Farida Ayuti, Siti Rani Azhar Azhar Azhari Azhari Basri Gani budianto panjaitan Cut Dahlia Iskandar Dani Rosdiana Darniati Darniati Daud AK, M. dwita, rina Eliawardani Eliawardani, Eliawardani Erdiansyah Rahmi Erina Erina Faisal Jamin Fakhrurrarzi, Fakhrurrazi Fakhrurrazi Fakhrurrazi Fema Amzani Nasution, Feby Fifia Chandra Fitriani Fitriani Ginting, Septian Tri Mulyana Hamdani Budiman Hamny Hamny Hasanuddin Hasanuddin Helmi, T. Zahrial Henni Vanda HENNIVANDA, HENNIVANDA Huriatul Masdar Husna Husna Ismail Ismail Jalaluddin, M M. Hasan M. Isa M. Nur Salim Mahdi Abrar Maryam Maryam Maryulia Dewi Matondang, Siti Wardianah Moliwati, Moliwati Momon Sodik Imanuddin Muhammad Hambal Muhammad Jalaluddin, Muhammad Mulyadi Adam, Mulyadi Mulyadi Mulyadi Muslina Muslina, Muslina Muttaqien Bakri, Muttaqien Muttaqien Muttaqien, Muttaqien Nasution, Borgo Mauly Nurdin, Hendri Nurhaspika, Nurhaspika NURLIANA NURLIANA Pratama Sekedang, M. Iqbal Ramadhani, Fitria Sari Rastina, Rastina Rastina, Rastina Razali Daud Razali Razali Reimena, Resti Reynaldi, Fitrah Reynaldi, Fitrah Riman Sipahutar Rinidar Rinidar, Rinidar Risa Tiuria Rosmaidar Rosmaidar, Rosmaidar Safika, Safika Safika, Safika Safika, Safika Safrizal Safrizal Sahputra, Dedy Samadi Samadi Saputri, Pragita Ayu Siti Aisyah Siti Hajar Siti Masreah Bernas Sugito Sugito Sulasmi Sulasmi Suyoto, Balqis Alrasyid Syafruddin Syafruddin Syahputra, Yandi T. Reza Ferasyi, T. Reza Teuku Reza Ferasyi Teuku Zahrial Helmi Teungku Nih Farisni, Teungku Nih Ummu Balqis Vara Tassa Sutari, Vara Tassa Veni Nella Syahputri, Veni Nella Waskito Waskito, Waskito Wenny Novita Sari Winaruddin Winaruddin xarolin, laras berlian Yarmaliza, Yarmaliza Yarmaliza, Yarmaliza Yuda, Sandy Cakra Yudha Fahrimal Yulina Rahmi, Yulina Yurliasni Yurliasni Zahra, Amalia Fatma Zakiah Heryawati Manaf, Zakiah Zakiyah Heryawati Manaf Zakiyuddin, Zakiyuddin Zulfan, Muhammad Zuraidawati Zuraidawati, Zuraidawati