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PERBANDINGAN AKTIVITAS ANTELMINTIK ALBENDAZOLE DAN LEVAMISOLE TERHADAP ASCARIDIA GALLI SECARA IN VITRO Balqis, Ummu; Hambal, Muhammad; Darmawi, .; Harris, Abdul; Rasmaidar, .; Athaillah, Farida; Muttaqien, .; Azhar, .; Ismail, .; Daud, Razali
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.142 KB) | DOI: 10.29244/avi.4.2.97-102

Abstract

Penelitian ini meneliti aktivitas antelmintik albendazole dan levamisole terhadap hambatan motilitas, percepatan waktu paralisis, dan motilitas cacing Ascaridia galli dewasa secara in vitro. Sebanyak empat ekor cacing masing dibuat triplikat dalam NaCl 0,9% masing-masing dengan konsentrasi 15 mg/ml Albendazole, dan 0.6 mg/ml Levamisole. Motilitas cacing diamati pada interval 10, 20, 30, dan 40 jam. Paralisis dan kematian diamati pada tampilan tidak ada pergerakan badan pada bagian kepala dan ekor cacing. Hasil menunjukkan bahwa aktivitas albendazole dan levamisole terhadap mortalitas A. galli berturut-turut terjadi pada 40 dan 30 jam pasca inkubasi. Levamisole dapat menghambat motilitas A. galli pada jam ke 10 dan juga menyebabkan lebih awal paralisis pada 6,75 ± 0,50 jam pasca inkubasi. Kajian tersebut mengindikasikan bahwa aktivitas antelmintik levamisole lebih awal dibandingkan efek albendazole pada cacing A. galli.
Isolation of gram-negative bacteria from metacarpal injury of Panthera tigris sumatrae trapped in Subulussalam, Indonesia Darmawi, .; Darniati, .; Manaf, Zakiah Heryawati; Syafruddin, .; Sayuti, Arman
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The present study aimed to isolate gram-negative bacteria infected metacarpal of Panthera tigris sumatrae trapped in Subulussalam, Aceh Province. Swab sample was obtained from metacarpal injury of P. tigris sumatrae. Swab sample was cultured to nutrient broth media using sterile cotton swabs or Pasteur pipettes, and incubated at 37°C temperature for 24 hours. Culture was spared on MacConkey media and incubated again at 37°C temperature for 24 hours. Determination of bacteria colony growth on the surface of MacConkey media based on shape, colour, surface, size, and viscosity (consistency). The bacteria colony stained with Gram staining, and tested biochemically.  The result showed that gram-negative bacteria such as Citrobacter sp., Proteus sp., Providencia sp., Pseudomonas sp.,and Salmonella sp. isolated from metacarpal injury of P. tigris sumatrae.
Poultry sellers’ perception and their effort for prevention strategy on avian influenza in Banda Aceh, Indonesia Darmawi, .; Ferasyi, Teuku R.; Muthmainnah, .; Balqis, Ummu; Fakhrurrazi, .; Darniati, .; Hambal, Muhammad
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

A study was conducted to examine some poultry sellers’ perception of the Avian influenza (birdflu) in nine locations (Ulee Kareng, Batoh, Lamnyong, Kampong Baru, Peunayong, Setui, Kampong Ateuk, and Neusu) in Banda Aceh Municipality, Indonesia. Primary data were obtained with the use of a structured questionnaire administared to ninety nine (99) respondents selected using proportional stratified random sampling, between November to December 2011. Information was obtained using the questionnaire sheets contain questions: knowladge, attitude, availability of facilities, and the effort of Avian influenza prevention strategy. Data obtained were analyzed by descriptive statistics. The result showed that only 52% of the poultry sellers’ had good knowledge of early symptomps of Avian influenza. Amount 59% of the poultry sellers’ had good attitudes. Result also showed that 73% of respondents had good facilities that was available in live poultry market area, but only 70% of respondents were good awareness about the effort of Avian influenza prevention strategy. In addition, the respondents who had more knowledge, good attitudes, and/or good facilities were also those who actually acted more preventively. Based on the result we concluded that the poultry sellers’ perception on prevention strategy for infection of birdflu in Banda Aceh categorized as poor. It is suggested that compliance with preventive measures may be enhanced through behavioural modification and building awareness through communication, training, workshop programs to the live poultry market sellers’ in Banda Aceh
Enzyme activity of Ascaridia galli Llarvae was inhibited by Phenil Methanyl Methane Sulfonyl Fluoride Balqis, Ummu; Darmawi, .; Hambal, Muhammad
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.955 KB)

Abstract

Enzyme catalyse a broad spectrum of important biological reactions, including protein metabolism and immune reactions. A study was carried out to partial characterize enzyme from exretory/secretory of A. galli L3 stage based on influence of inhibitor. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. Excretory/secretory was prepared from metabolic product of L3 released in culture medium. The enzyme activity was assayed against casein 2%. The amount of concentration was determined from the absorbance at 595 nm. Inhibitor sensitivity on enzyme activity was studied in phenil methanyl methane sulfonyl fluoride (PMSF) 0,5 and 1 mM.    The result showed that enzyme activity of A. galli larvae was inhibited by PMSF 0.5 and 1 mM, which enzyme activity remainded 1,8 and 0%, respectively. The results indicate that the excretory/secretory secreted by L3  A. galli contained serine protease
The effect of excretory/secretory product released by L3 of A. galli on villous compact in intestine of laying hens Balqis, Ummu; Darmawi, .; Tiuria, Risa; Priosoeryanto, Bambang P.
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The nematode, Ascaridia galli, caused pathological changes in the gut, while the larval development takes place in the intestine epithelium. The aim of this research was to examine the effect of excretory/secretory product released by L3 of A. galli to prevent intestine destructions based on villous compact in duodenum, jejunum, and ileum of laying hens. Excretory/secretory was prepared from metabolic of L3 released in culture médium in flasks containingrosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Excretory/secretory product was concentrated with vivaspin 30.000 MWCO. The chickens vaccinated with 80 >g protein excretory/secretory in crudemixed with Fruend Adjuvant Complete and repeated three times with dose of each 60 >g mixed with Freund Adjuvant Incomplete with an interval of one week intra muscularly. The chickens were challenged with 1000 infective larvae A. galli one week later. After two weeks the chickens were operated. Villous compact in duodenum, jejunum, and ileum of laying hens were analyzed. The result showed that immunization was able to sustained significantly intestinevillus compact, particularly in duodenum. This research concluded that the excretory/secretory product could protect villus in duodenum against parasitic diseases caused by A. galli.
THE ABILITY OF IMMUNOGLOBULIN YOLK RECOGNIZED THE ANTIGEN IN THE TISSUE OF ASCARIDIA GALLI Darmawi, .; Balqis, U; Hambal, M; Tiuria, R; Priosoeryanto, B P; Handharyani, E
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1489.954 KB) | DOI: 10.5398/medpet.2012.35.3.190

Abstract

Antigen-antibody reaction is an important tool for the analysis of localization of target molecules, including antigenic protein within worm tissues. The purpose of the present research was to demonstrate the ability of immunoglobulin yolk (IgY) anti-excretory/secretory recognized the antigen in the tissue of Ascaridia galli by mean of immunohistochemistry method. The excretory/secretory protein was procured from A. galli and concentrated by mean of vivaspin 30,000 MWCO. IgY was produced by egg yolks of immunized chickens with excretory/secretory, and purified using fast protein liquid chromatography (FPLC) method. A. galli adult worms were cut in transversal and longitudinal section of the center and anterior region. Slides were incubated with both primary IgY for overnight at 4 oC and secondary antibody rabbit anti-chicken IgY HRP-conjugate for one hour at room temperature. The slides were stained with 3-amino, 9-ethylcarbazole (AEC) chromogen, counterstained with Lillie Mayer Haematoxylin, and mounted in glyserin aqueous mount.  Antigen-antibody reaction was investigated under a microscope. The result showed that antigen was appeared in the tissues such as cuticle, epicuticle, buccal cavity, and eggs inside the uterine of A. galli. This research concluded that IgY stimulated by the excretory/secretory was able to recognized the antigen scattered in the tissues of A. galli so the IgY could be applied for immunodiagnostic.
Isolation of enterobacteriaceae bacteria species from feces of Sumatran Orangutans (Pongo abelii) Darmawi, .; Rahmi, Erdiansyah; Dewi, Maryulia; Hutabarat, Joharsyah; Abrar, Mahdi; Fakhrurrazi, .
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The porpouse of this research aimed to isolate enterobacteriaceae bacteria from feces of Pongo abelii. The samples of feces were collected from 15 captive orangutans in Orangutan Sumatera Batu Mbelin Sibolangit Qurantine, North Sumatra. Of each sample was cultured in nutrient broth media using sterilecotton swabs or Pasteur pipettes, and incubated at 37°C temperature for 24 hours. Culture was spared on Methylene Blue Agar (Oxoid), examined by Gram staining, and tested by biochemically. The result showed that significantly more common appear Escherichia sp. (93,33%) and fewer Edwardsiella sp. (66,67%) wereisolated from feces samples of P. abelii. Others enterobacteriaceae found in feces of P. Abelii were Shigella sp. (46,67%), Klebsiella sp. (33,33%), Citrobacter sp., and Salmonella sp. (13,33%), respectively
Histopathological changes in intestine of chicken (Gallus domesticus) infected naturally by Ascaridia galli Balqis, Ummu; Hambal, Muhammad; Darmawi, .; Utami, Cut S.
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This study was aimed at finding out the histopathological changes of chicken intestines that were naturally infected by Ascaridia galli. Ten intestines were obtained from wet market in Banda Aceh then measured in length and divided into three sections: duodenum, jejunum and ileum, of which histologicslides were made. The number of Ascaridia galli founded in the lumen and ingesta were counted. Parameter picture of histopathologycal changes were analized descriptively based on desquamation, hiperplasia, and villi fussion occure. Duodenum, jejunum and ileum infected by A. galli showed istopathological changes of villi the cover of desquamation, hyperplasia and fusion. Demage level of the small intestine chicken was largely determined by the number of infecting by A. galli. It was concluded that the more number of A galli infected to chickens, the higher level of damage would be occured in the small intestine of chicken
MUCOSAL MAST CELLS RESPONSE IN THE JEJUNUM OF ASCARIDIA GALLI-INFECTED LAYING HENS Darmawi, .; Balqis, U; Hambal, M; Frengki, .; Priosoeryanto, B P
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.113 KB) | DOI: 10.5398/medpet.2013.36.2.113

Abstract

Intestinal defense mechanism against helminthes parasitic nematode to be associated with mucosal mast cells reaction. The aim of this research was to examine the effect of infection by Ascaridia galli parasite to trigger mucosal defense based on mucosal mast cells response in laying hens. Amount of ten head laying hens 12-wk old were divided into two groups containing five chickens of each. The first group, chickens were left as un-infected controls. The second group, chickens were infected orally with 1,000 embryonated eggs of A. galli.  Mucosal mast cell responses were assayed by in situ jejunal mast cell counts in stained serial histological sections with Alcian blue (pH 0.3) and Safranin-O (pH 0.1) of the jejunum. Mucosal mast cells response were observed and counted on days 14 post infection. The result showed that A. galli infection was able to increase significantly (P<0.05) mast cells response. This research concluded that the A. galli infection can trigger the involment of mucosal mast cells response in jejunal defense of laying hens against parasitic diseases caused by A. galli.
Infiltration of inflammatory cells in intestines of chickens infected naturally by Ascaridia galli Hambal, Muhammad; Balqis, Ummu; Darmawi, .; Utami, Cut S.
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.327 KB)

Abstract

This research was carried out to investigate the infiltration of inflamatory cells in the intestines that were naturally infected by Ascaridia galli. Ten intestines were obtained from wet market in Banda Aceh. Inflamatory cells were assayed by in situ jejunal mast cell counts in stained histological sections of the duodenum, jejunum and ileum, of which histologic slides were made. Duodenum, jejunum and ileuminfected by Ascaridia galli showed hyperemia, and inflammatory cell infiltration in part of A. galli infection