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ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE STAPHYLOCOCCUS HOMINIS PADA ONCOM MERAH PASCA FERMENTASI 120 JAM Harun, Aulia; Muchlissin, Sakti Imam; Mukaromah, Ana Hidayati; Darmawati, Sri; Ethica, Stalis Norma
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Enzymes are complex protein moleculer produced by living cells playing role as catalysts in various chemical processes in the body. Among enzymes playing an important role in human life is protease. The purpose of this study was to determine the presence of protease ? producing bacteria found on 120-h post - fermented oncom and to identify the bacteria based on its 16S   rRNA gene analysis. Bacterial isolation and purification was carried out using Nutrient Agar media with spread technique. Of the six bacterial isolates isolated from the oncom sample after 120 hours of fermentation, there was one isolate that had protease activity, namely IROD 5. The protease enzyme income test was carried out using Skim Milk Agar media. Molecular identification process was carried out through sequential analysis of 16S rRNA using PCR method using primers forward F: 5'-AGAGTTGATCCTGGCTCAG-3 'and reverse R: 5'- GGTTACCTTGTTAC. GACTT-3 primers' followed by sequencing process. The protease enzyme production test to bacterial isolate was conducted using Skim Milk Agar. Molecular identification was performed through analysis of 16S rRNA gene sequence using PCR method followed by sequencing process. A single bacterial isolate having proteolytic activity was obtained based on observation of the clear zone of protease surrounding the bacterial colony with a diameter of 72 mm. The 16S rRNA gene sequence of the obtained proteolytic bacterial strain IROD5 has been obtained and analysis on the gene sequence resulted 99% similarity levels with sequence of similar gene s of Staphylococcus hominis. As conclusion, the obtained bacterial isolate in this studyis apotential protease  enzyme  producer  and  molecularly  identified  as  Staphylococcus hominis strains IROD5. Keyword : Protease Enzyme, Gen 16S rRNA, Red Oncom
Analisis Molekuler Profil Protein Pilli untuk Mengungkap Hubungan Similaritas 26 Strain Salmonella typhi Isolat Jawa Darmawati, Sri; Haribi, Ratih; Anwar, Syaiful
PROSIDING SEMINAR NASIONAL 2012: SEMINAR NASIONAL HASIL PENELITIAN 2012
Publisher : Universitas Muhammadiyah Semarang

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Variasi dan hubungan similaritas 26 strain Salmonella typhi Isolat Jawa merupakanawal untuk melacak protein sub unit pilli spesifik yang memiliki aktivitas hemaglutinasi.Tujuan penelitian analisis molekuler profil protein pilli untuk mengungkap hubungansimilaritas 26 Strain S. typhi Isolat Jawa.Analisis dilakukan terhadap 26 strain yang berasal dari Surabaya, Madiun,Malang, Salatiga, Magelang, Bandung, Bogor, Jakarta, Yogyakarta dengan elektroforesisSDS-PAGE. Analisis hubungan similaritas digunakan program MVSP, untukmengkonstruksi dendogram yang mencerminkan klasifikasi dari 26 strain S. typhi IsolatJawa berdasarkan nilai indeks similaritas (S SM ) dengan algoritma UPGMA.Hasil analisis profil protein pili menunjukkan (1) Jumlah pita protein sub unitpilli bervariasi : 8-17 pita, BM tertinggi 200 kD, terendah 10 kD, dengan 20 karakter. (2)Protein 100 kD, 50 kD, 45 kD dan 40 kD adalah protein sub unit pilli yang dimiliki oleh26 strain S. typhi Isolat Jawa. (3) Dari 26 strain S. typhi Isolat Jawa terdiri dari duakelompok besar yang mempunyai indeks similaritas 61,2%. Kelompok pertama adalahstrain S. typhi Isolat Jawa Tengah dan Jawa Timur, dan kelompok ke dua adalah strain S.typhi Isolat Jawa Barat dan DKI. Pita 14 (12,5 kD) dan 15 (78kD) dari protein sub unitpilli hanya dimiliki strain S. typhi dari Surabaya. Pita 18(35kD) dan 20 (72kD) dariprotein sub unit pilli hanya dimiliki oleh Strain S. typhi dari DKI Jakarta.
IDENTIFIKASI DAN HITUNG JUMLAH BAKTERI KONTAMINAN PADA LALAT Masca domestica BERDASARKAN LOKASI PENANGKAPAN DI RUMAH SAKIT BHAYANGKARA SEMARANG Darmawati, Sri; -, Sayono; Sudarmadi, Misno
JURNAL KESEHATAN MASYARAKAT INDONESIA Vol 2, No 2 (2005): JURNAL KESEHATAN MASYARAKAT INDONESIA
Publisher : JURNAL KESEHATAN MASYARAKAT INDONESIA

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Latar belakang: Lalat, khususnya lalat rumah (Musca domestica) merupakan salah satu vektor mekanis beberapa jenis penyakit. Aktivitas berkembang biak pada sampah dan mencari makan pada makanan manusia berpotensi menimbulkan kontaminasi bakteri pada makanan dan minuman. Di lingkungan rumah sakit, hal ini sangatpenting untuk diperhatikan. Tujuan: mengidentifikasi bakteri dan menghitung jumlah bakteri kontaminan yang terdapat pada tubuh lalat.Musca domesetica. Metode: Jenis penelitian ekplanatori, dengan rancangan cross sectional dan pendekatan observasional. Populasi penelitian adalah lalat Musca domestica yang berada di lingkungan rumah sakit Bhayangkara Semarang. Sampel diambil secara accidental (kebetulan). Lalat ditangkap dengan jaring penangkap lalat. Variabel bebas, yaitu tempat (terdiri dari 3 lokasi), dan variabel terikat adalahjumlah bakteri kontaminan pada tubuh. Jumlah bakteri dihitung dengan metod Standart Plate Count (SPC). Data diolah dengan program komputer dan dianalisis secara diskriptif dan analitik. Analisis analitik menggunakan uji Analisa Varians Satu Jalan (One Way Anova). Hasil; Hasil identifikasi ditemukan, lima jenis bakteri kontaminan adalah Providencia rettgeri, Providencia stuartii, Enterobacter aerogenes, Citrobacter freundii, dan Bacillus sp., rerata jumlah bakteri pada lalat yang ditangkap di dapur adalah 1,85 x 104 sel batkteri/ekor, di TPS 7,4 x  103sel bakteri/ekor dan di bangzaal perqwatan 1,93 x 103 sel bakteri/ekor, hasil uji Anova menunjukkun nilai F = 1,142 dan p : 0,336. Simpulan: Ada lima jenis bakteri kontaminan yang ditemukan pada tubuh lalat di lingkungan rumah sakit Bhayangkara Semarang, tidak ditemukan bakteri Salmonella sp, dan tidak ada perbedaan jumlah bakteri secara signifikan menurut tempat penangkapan lalat.Kata kunci: Musca domestica, bakteri kontaminan, rumah sakit
ANALISIS PROTEIN PILLI Salmonella typhi lsolat RS. Kariadi Semarang DENGAN ELEKTROFORESIS SDS-PAGE Darmawati, Sri; Haribi, Ratih
JURNAL LITBANG Vol 2, No 3 (2005): Penelitian, Pengembangan, dan Pengabdian
Publisher : JURNAL LITBANG

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Background : Salmonella typhi is bacteria which causes typhoid fever. Typhoid fever is severally serious, infectious decease and is endemic decease in Indonesia with relatively high frequency rate around 358-810/100.000 people each year This decease is usually spreading along with many kinds of decease which also have relatively high mortality rate, that is 1-5% of the sufferers (Punjabi, NH. , 2 004) . The infection process of bacteria into human body is signified with bacteria Cell adhering in mucosa intestinal surface. Pilli is structured by pilli protein consisting of Several sub units of pilli protein Objective: conducting pilli protein Salmonella typhi isolate Kariadi Hospital Semarang Analysis through electrophoreses SDS-PAGE, so that take amount of sub unit of pilli protein with each its molecule weight can be found out. Method : This research is conducted through three stages; firstly, bacteria cultivation with Biphasic media (BHI Agar and broth BHI ); secondly, pilli isolation with Ehara method (/956); Thirdly, sub unit of pilli protein separation with I 2% SDS-PAGE based on Lemmli method (1970). Result: There are four major sub units of protein in pilli, that is, 36kDa; 26,5 kDa; 22,2kDa; 18,6lrDq and there are also four minor sub units of protein pilli, that is, 116 kDa; 62,3kDa;45kDa;20,9kDa,andseveral other minor sub units of protein with every thin band. Keywords : Salmonella and Electrophoresis
PERBEDAAN VARIASI LAMA SIMPAN TELUR AYAM PADA PENYIMPANAN SUHU ALMARI ES DENGAN SUHU KAMAR TERHADAP TOTAL MIKROBA ., Idayanti .; Darmawati, Sri; Nurullita, Ulfa
JURNAL KESEHATAN Vol 2, No 1 (2009): Pengembangan Ilmu-Ilmu Kesehatan
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Abstract Background; Chickens Egg is one of animal producl coming from poultry livestock and well-known as food materials with high protein source and many people consume it. Chickens egg quality can be in Influenced by keeping place, temperature, dampness, dirt at handling technique and eggshell. Egg can be hit by microbe pollution coming from pollution result both direct and indirect contamination. Habit of keeping chickens egg forfew dalts al room temperature can cause the egg is easy lo be contaminated by microbe, so lhat the egg quality is easy to destroy or decay. Besides il is oflen done of keeping egg in refrigerator, expected the egg will be more durable.This research aim stok now the diffirence of keeping variation long that is0,6, l2, and l8 drys at refrigerator lemperature with room lemperature to total microbe. Method : This research is pure experiment using device of One Group Pretest - Postest. Research object counted 42 chickens eggfor pretest is 0 day with restaling one egg so it needs 6 eggs, then as postest is 3 keeping treatment (6, 12, and l8 days), 2 measurement of lemperature (refrigerator temperature mean 40C with room temperature mean 290C) and 6 times restating. Independen variable is long save variation and temperature, dependent variable is total mikkrobe, Statistic calculation is done with SPSS l4/3 windows program Version 11 .0 using factorial test or Two Way Anova with d 0,05. Result : Mean total of microbe al refrigerator temperature with room temperature depend on keeping variation long to experience of significant dffirence to lolal microbe, P<0,05 depend on value of p seen there is significant difference al keeping variation long of chickens egg at refrigerator temperature with room temperature to total microbe. Conclusion : Total microbe al keeping varialion long 0, 6, 12, and 18 days progressively increase signiftcantly both at refrigerator or room temperature
Penggunaan self cleaning Fotokatalis Tio2 dalam Mendegradasi Ammonium (NHd) Berdasarkan lama waktu penyinaran Mukaromah, Ana Hidayati; Amin, Muh.; Darmawati, Sri
JURNAL KESEHATAN Vol 3, No 1 (2010): Ilmu-Ilmu Kesehatan
Publisher : JURNAL KESEHATAN

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Ammonium is NH a ions thdt are not colored, smelly and dangerous to health, its concentrqtion determined by spectrophotometric method. Ammonium which is atkalini when exposed i tignt or heat will cause odor, because the smell of ammonia generated, needed a technologt to reiuce or eliiinate the levels of ammonium. Problems of this research is what percentage of degrqdalion of ammonium (NH4 +) with 20 mg of photocatalyst TiO 2 based on the exposure time?The general obiective of this research is to study the degradation of ammonium (NH 4 +) with TitaniumDiol<sida photocatalyst (TiO 4 20 mg based on exposureiime 30, 60: g0, 120, 240, 360, 480, 600, 900, 1500 minutes. Special purpose in this study are: Peiform initial optimization siudy is determine the optimum concentration of ammonium that can produce the mmimum percent ammonium degradation with the number of photocatalyst TiO 2 Titanium Dioxide 20 mg durig the time of 120 minutes. Doing degradation of ammonium with ammonium concentrqtion optimim withlhe number of photocatalyst TiO 2 2b mg for varying exposure time 30, 60, 90, 120, 240, 360, 480, 600, 900, I500 minures.The research object is a solution of ammonium produced in the chemical laboratory of the concentration of 100 ppm was reduced to 10, 20, i0, 40 ppm and then determined the optimui concentration of ammonium. Percent degradation of ammonium with an optimum concentration wiih the addition of titanium dioxide photocatalyst TiO 2 20 mg with varying exposure time 30, 60, 90, t 20, 240, 360, 480, 600,-900, t 500minutes each performed three times repetition.The results showed that the optimum concentration of ommonium NHr*) with photocatalytic TiO2 20 mg over 120 minutes is 30 ppm. Degradation of the ion (NHr) with the variation ofradiarion SO, AO, g0, 120, 240, 360, 180, 600, 900, and 1500 minutes with the optimum concentration of 3i ppm of ammonium and the number of photocatalyst TiO 2 20 mg is five consecutive, 66ok, 6.06%, 6.64%, Z.iZbZ, A.Otm, g.64%, g.5g%,10.52%o, ll.0B%, 11.40%. The longer the exposure time the greater the percent degradation of the ion (NH4 +)Keywords: Degradation of ammonium, Tio2 Photocatalyst, Irradiation time.
KELAINAN FUNGSI HATI DAN GINJAL TIKUS PUTIH (Rattus norvegicus, L.) AKIBAT SUPLEMENTASI TAWAS DALAM PAKAN Haribi, Ratih; Darmawati, Sri; Hartiti, Tri
JURNAL KESEHATAN Vol 2, No 2 (2009): Ilmu-Ilmu Kesehatan
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Abstract Alum is used to improve the quality of food containing toxic heavy metal ions which can interfere with Aluminum enzymatic system, and tissue damage. Liver and kidney are the most used network is affected, because it is a detoxification organ. Liver and kidney damage can be detected by an enzyme concentration of SGOT, SGPT, Billirubin, Protein, Ureum and Creatinin in the blood This study aims to find out the effects of alum in a feed supplement for liver and kidney damage in a clinic conducted from May to Oclober 2007, at the Laboratory of the University Clinic Patologt Muhammadiyah Semarang. Sample studies of white rats (Rattus norvegicus, L.), aged 2 months with weight average of 200 grams. 0o/o dose treatment (without supplementation), 0.05%, 0.1%, 0.2%, 0.5%, 1% and 0% (without supplementation), and subsequent treatment with a dose of 2%, 3%, 4%, 5 % and 6% volum, who every day put into the stomach of rats l0 mL Clinical laboratory tests performed at the time before treatment (control), 4 weeks, 6 weeks and 8 weeks of exposure time. Examination AST and ALT with Ultra Violet Test methods, Total Billirubin with modifications Groff Jendrasik method, total protein Colorimetri method, U Berthelot method, Creatinin Jaffe method. Clinical chemistry tests showed that supplementation influence of alum on the concentration of Enzymes and other factors in the blood of mice associated with damage to liver and kidney tissue. Level of organ damage significant with alum in a feed supplement. The higher the concentration of alum disuplementasion and the longer exposure time resulted in damage to the liver and kidneys getting worse.
PROFIL PROTEIN DAGING KAMBING, KERBAU DAN SAPI YANG DIRENDAM LARUTAN JAHE BERBASIS SDS-PAGE Fadhila, Rieke; Darmawati, Sri
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Ginger contains protease enzyme and that is Zingibain which can hydrolyze proteins so it can soften meat. The aim of this study is to analyze protein profiles in goat, buffalo and cow meats before and after those meats are soaked with ginger solution in 4% v/v, 6% v/v, 8% v/v and 10% v/v concentrations for 30 minutes. Protein profile of meats was analyzed with SDS-PAGE method. The result of this study shows that control meats which are not soaked with ginger solution have many major protein bands while sample meats which have soaked with ginger solution have many minor protein bands. The result of this study shows thatsoaking meats with ginger solution can soften meats because zingibain enzyme in ginger solution can break peptide bonds in protein of meat so it forms micromolecules (minor bands). Ginger solution which is the best to soak goat, buffalo and cow meats is on 4% concentration because there are so many proteins in those meats. The higher ginger solution concentration is the more and more zingibain enzyme content are so the ability to hydrolize protein is higher marked with slackening major protein bands and increasing minor protein bands. Keywords: Meat, Ginger, Protein Profiles, SDS-PAGE
PROFIL PROTEIN TIGA JENIS DAGING YANG DILUMURI SERBUK BUAH MENGKUDU BERBASIS SDS-PAGE M, Wa Ode Jariah; Darmawati, Sri
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Noni contains protease enzymes that can hydrolyze proteins by breaking the peptide bond, so it can be used to soften the meat. The purpose of this study was toanalyze protein profile on 3 types of meat (goats, buffalo and cow) before and aftergreased with non-fertilizing powder with 30 minutes immersion. Protein profile of three meat types was analyzed using the SDS-PAGE method. The design of this research is descriptive research with the object of research are goat meat, buffalo and cow greased with powder of noni. The results showed that in control meat (goats, buffalo and cow) that were not greased with noni powder there were many major protein bands compared with minor protein bands. While on goat meat, buffalo and cow greased with powder of noni with concentration 10% b / b, 15% b / b, 20% b / b and 25% b / b showed different results at each concentration that there were many minor protein bands than the major protein bands. The higher concentration powder of Noni then the amount of protein in the meat is more denatured. Based on these results indicate that the protease enzyme contained in the powder of noni is able to break peptide bonds in meat protein to proteinshaped minor bands (micromolecul).Keyword : meat, noni powder, protein profile, SDS-PAGE
ANALISIS MOLEKULER PROFIL PROTEIN PILLI UNTUK MENGUNGKAP HUBUNGAN SIMILARITAS 26 STRAIN Salmonella typhi ISOLAT JAWA Darmawati, Sri; Anawar S, -; Artama, W T
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2010: PROSIDING SEMINAR NASIONAL HASIL-HASIL PENELITIAN
Publisher : Universitas Muhammadiyah Semarang

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Variasi dan hubungan similaritas 26 strain Salmonella typhi Isolat Jawa merupakan awal untuk melacak protein sub unit pilli spesifik yang memiliki aktivitas hemaglutinasi. Tujuan penelitian analisis molekulerprofil protein pilli untuk mengungkap hubungan similaritas 26 Strain S. typhi Isolat Jawa. Analisis dilakukan terhadap 26 strain yang berasal dari Surabaya, Madiun, Malang, Salatiga, Magelang, Bandung, Bogor, Jakarta, Yogyakarta dengan elektroforesis SDS-PAGE. Analisis hubungan similaritasdigunakan program MVSP, untuk mengkonstruksi dendogram yang mencerminkan klasifikasi dari 26 strain S. typhi Isolat Jawa berdasarkan nilai indeks similaritas (S SM ) dengan algoritma UPGMA. Hasilanalisis profil protein pili menunjukkan (1) Jumlah pita protein sub unit pilli bervariasi : 8-17 pita, BM tertinggi 200 kD, terendah 10 kD, dengan 20 karakter. (2) Protein 100 kD, 50 kD, 45 kD dan 40 kD adalah protein sub unit pilli yang dimiliki oleh 26 strain S. typhi Isolat Jawa. (3) Dari 26 strain S. typhi Isolat Jawa terdiri dari dua kelompok besar yang mempunyai indeks similaritas 61,2%. Kelompok pertama adalah strain S. typhi Isolat Jawa Tengah dan Jawa Timur, dan kelompok ke dua adalah strain S. typhi Isolat Jawa Barat dan DKI. Pita 14 (12,5 kD) dan 15 (78kD) dari protein sub unit pilli hanya dimiliki strain S. typhi dari Surabaya. Pita 18(35kD) dan 20 (72kD) dari protein sub unit pilli hanya dimiliki oleh Strain S. typhi dari DKI Jakarta.Kata kunci: Protein Pilli, Hubungan similaritas, Salmonella typhi