Endang Darmawan
Fakultas Farmasi Universitas Ahmad Dahlan, Jl. Prof. Dr. Soepomo S.H., Janturan Umbulharjo, Yogyakarta

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Efek Serbuk Bunga Rosella Merah (Hibiscus sabdariffa L.) terhadap Ekspresi IL-10 pada Sukarelawan Sehat Syahrana, Nur Azizah; Akrom, Akrom; Darmawan, Endang
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol 4, No 1 (2017): Jurnal Farmasi dan Ilmu Kefarmasian Indonesia
Publisher : Fakultas Farmasi Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.983 KB) | DOI: 10.20473/jfiki.v4i12017.1-5

Abstract

Pendahuluan: Bunga rosella merah (Hibiscus sabdariffa L.) mengandung fenol dan flavonoid antosianin yang memiliki aktivitas sebagai antioksidan dan dapat meningkatkan sistem imun. Tujuan: Tujuan penelitian ini untuk mengetahui pengaruh kapsul serbuk bunga rosella terhadap ekspresi IL-10 menggunakan metode pre and post test pada 21 sukarelawan sehat yang memenuhi kriteria inklusi dan bersedia mengisi informed consent. Metode: Relawan diberikan serbuk rosella dalam bentuk kapsul dengan dosis 500 mg selama 30 hari dan dilakukan pemeriksaan IL-10 menggunakan metode flowsitometri pada hari ke-0, 30 dan 45. Hasil dianalisis menggunakan SPSS dengan uji wilcoxon. Hasil: Nilai ekspresi IL-10 pada sukarelawan hari ke 0, ke-30 dan ke-45 adalah 0,42 ± 0,24; 0,83 ± 0,47 dan 1,06 ± 0,67. Hasil ini menunjukkan ekspresi IL-10 bepengaruh pada hari ke-0 ke-30 (p < 0,05) dan ekspresi IL-10 tidak berpengaruh hari ke-30 ke-45 (p > 0,05). Kesimpulan: pemberian kapsul serbuk rosella (Hibiscus sabdariffa L.) dosis 500 mg/hari selama 30 hari meningkatkan ekspresi IL-10, tetapi dalam batas nilai normal pada sukarelawan sehat.
Synergistic antibacterial activity Combination of Syzygium aromaticum L. and Myristica aragrans Houtt. Against Staphylococcus Aureus ATCC 25923 and Their Chemical Consituents Fauziah, Dewi Winni; Darmawan, Endang
Jurnal Farmasi dan Ilmu Pengobatan Vol 2 No 2 (2017): JPMS
Publisher : STIFA Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (504.327 KB)

Abstract

Infeksi dapat disebabkan oleh bakteri yang salah satunya adalah Staphylococcus aureus yang merupakan bakteri gram positif. Pengobatan infeksi bakteri banyak dilakukan dengan menggunakan antibiotika atau minyak dari bahan alam. Minyak atsiri dari Syzygium aromaticum L. dan Myristica fragrans Houtt memiliki aktivitas antibakteri terhadap bakteri gram positif. Penelitian ini bertujuan untuk mengetahui sinergisme aktivitas antibakteri kombinasi minyak atsiri dari Syz&nbsp; dan Myr terhadap bakteri S. aureus ATCC 25923. Uji sinergisme aktivitas antibakteri dilakukan menggunakan metode mikro dilusi checkerboard dengan penambahan MTT sebagai indikator pertumbuhan bakteri. Efek kombinasi dilihat dari indeks FIC dan Isobologram sedangkan komponen kimia minyak atsiri dari kedua tanaman tersebut dianalisis dengan GC-MS. Hasil uji aktivitas sinergisme memperlihatkan efek aditif terlihat pada perbandingaan Myr/Syz sebesar 2/5 dan 8/5 dengan indeks FIC sebesar 0,75; akan tetapi pada perbandingan Myr/Syz sebesar 2/5 memperlihatkan hasil indifferent dengan indeks FIC sebesar 1,5. Perbandingan konsentrasi terbaik antara Myr dengan Syz&nbsp; adalah 1 : 2,5 (mg/mL). Hasil analisis GC-MS menunjukkan komponen senyawa kimia utama dari minyak atsiri Syz adalah caryophyllene dan eugenol; minyak atsiri Myr adalah alpha pinene, beta-pinene, sabinene, p-cymene, terpinene-4-ol, safrol, dan miristisin. Secara keseluruhan dapat disimpulkan bahwa kombinasi minyak atsiri kedua tanaman tersebut memiliki efek aditif dalam menghambat S. aureus ATCC 25923.
Black Cumin Seed Oil Antidiabetogenic by Increasing Pancreatic P53 Expression Akrom, Akrom; Nurfadjrin, Rizma; Darmawan, Endang; Hidayati, Titiek
International Journal of Public Health Science (IJPHS) Vol 7, No 3: September 2018
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v7i3.13694

Abstract

Black cumin seed oil (BCSO) contains many thymoquinone and unsaturated fatty acids that act as antioxidants. Thymoquinone was thought to inhibit pancreatic damage and prevent hyperglycemia by involving p53. The study aimed to determine the BCSO effect on Malondialdehyde (MDA) levels and p53 expression of pancreatic tissues in alloxan-induced Sprague Dawley (SD) rats. This study used Pre and Post Test with Control Group Design with 49 male Sprague Dawley rats aged 30 days weighing 150 - 300 gram. Rats were divided into 7 groups: Group I, the normal control group receiving standard feeding and drinking; Group II, the negative control group receiving alloxan; Group III &amp; IV, the treatment groups, receiving BCSO with a dose of 6.9 mg/KgBW (BCSO6.8) and 68 mg/KgBW (BCSO68 group); Group V, the positive control 1 group receiving simvastatin 10 mg/KgBW; Group VI, the positive control 2 group receiving vitamin C 18 mg/200mgBW; Group VII, the solvent control receiving DMSO. The treatment groups received two different doses per day for 9 days orally. Blood MDA and glucose levels were determined by spectrophotometry and p53 expression of pancreas tissues were read by a pathologist. The results show that the BCSO68 group had a decreased activity of MDA and glucose but a higher expression of p53 in pancreatic tissues compared to the BCSO6.8 group. Decreased Malondialdehyde levels in the BCSO68 group were similar to those in the vitamin C group but lower than those in the simvastatin group (p&lt;0.05). It can be concluded that the BCSO administration of 68 mg/KgBW per day can decrease blood Malondialdehyde and glucose levels and increase p53 expression. 
PENGGUNAAN POTENTIALLY INAPPROPRIATE MEDICATIONS (PIMs) PADA PASIEN GERIATRI RAWAT INAP OSTEOARTHRITIS DI RS PKU MUHAMMADIYAH YOGYAKARTA Namirah Muh. Syuaib AS, Andi; Darmawan, Endang; Mustofa, Mustofa
Pharmaciana Vol 5, No 1 (2015): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.48 KB) | DOI: 10.12928/pharmaciana.v5i1.2289

Abstract

Potentially inappropriate medications (PIMs) use among elderly is a serious public health problem because it is intrinsically linked to increased morbidity, mortality, and healthcare costs. This study  aims to determine the association between number of drug prescribed, duration of hospital stay and PIMs use among elderly hospitalized patients at PKU Muhammadiyah Yogyakarta General Hospital. This study was analytic observational with cross-sectional study. Beers criteria 2012 were used to identify PIMs use among elderly hospitalized patients with osteoarthritis. Logistic regression analysis were used to determine association between number drugs of prescribed, duration of hospital stay and PIMs use. The results showed that 7 (38.9%) out of 18 hospitalized elderly patients were identified PIMs use and all of patients received atleast one PIM. The most common agents involved in PIMs were estazolam (11.1%), and metoclopramide (11.1%). Polypharmacy is a major predictor factor of PIMs use in this study.
Black Cumin Seed Oil (Nigella Sativa L) as Hepatoprotector in decreasing SGPT and SGOT activity and increasing p53 gene expression in rats induced by Alloxan Akrom, Akrom; Darmawan, Endang; Yuhelvia, Liesma
International Journal of Public Health Science (IJPHS) Vol 4, No 3: September 2015
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.512 KB) | DOI: 10.11591/ijphs.v4i3.8467

Abstract

Alloxan is one source of active radicals. Active radicals exposure cause oxidative stress which resulted in damage to cells or tissue . One organ that is susceptible to damage due to the active radical exposure is the liver. How MBJH effect on liver function and expression of P53 in SD rats induced by alloxan has not been widely studied. The research objective was to determine the MBJH effect on SGPT / SGOT levels  and P53 expression in SD rats induced by alloxan. This study uses a posttest only control group design . A total of 42 SD rats were divided into 6 groups . Group I (normal) . Group II (negative control) , were given alloxan. Group III and IV (treatment) were given MBJH equivalent doses of 6.8 mg / kg and 68 mg / kg thymoquinone . Group V (positive control) were given vitamin C. Group VI (control media) were given DMSO. MBJH and Vitamin C are given for 1 week. Alloxan given on 7th day,  intraperitoneally. On the 8th day do blood sampling for measurement of SGPT and SGOT levels . Liver retrieval is done on the 9th day continued with making preparations and Immunohistochemistry staining. The average levels of SGPT / SGOT were analyzed with ANOVA test. Percent of p53 expression was analyzed by Kolmogorov Semirnov and Levene then followed by Kruskal Wallis and Mann - Whitney. Statistical tests performed with 95% confidence level. The results showed that the induction of alloxan increase levels of SGPT /SGOT. MBJH equivalent dose of 68 mg / kg proven to reduce levels of SGPT /SGOT alloxan -induced mice ( p &lt; 0.05 ) . MBJH equivalent doses of 6.8 and 68 mg / kg thymoquinone also shown to increase the expression of P53 in liver tissue SD rats induced alloxan. Average percentage of P53 expression in Group I to Group VI respectively were 11.12 ± 0.37 ; 19.24 ± 4.47 ; 30.31 ± 1.59 ; 40.43 ± 1.74 ; 29.67 ± 0.47 ; 12.02 ± 1.01. Results of statistical analysis show the value of p &lt; 0.05 in all groups except in group III                Based on the results of this study concluded that MBJH are hepatoprotective and increased the expression of p53 in the liver of Sprague Dawley rats induced by Alloxan . Keywords: oxidative stress, alloxan, minjak black cumin seeds (Nigella sativa L), P53, Immunohistochemistry
EVALUASI PENGARUH PEMBERIAN KONSELING DAN SHORT MESSAGES SERVICE (SMS) TERHADAP KEPATUHAN TERAPI HIPERTENSI PASIEN HEMODIALISIS DI RSUD BANJAR Kurniasih, Nia; Supadmi, Woro; Darmawan, Endang
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Prosiding Seminar Nasional "Perkembangan Terbaru Pemanfaatan Herbal Sebagai Agen Preventif Pada Ter
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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Abstract

ABSTRAK Kepatuhan terapi hipertensi pasien hemodilisis dapat ditingkatkan dengan pemberian edukasi. Edukasi dapat diberikan dalam bentuk konseling langsung atau pengiriman Short Messages Service (SMS). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian konseling dan Short Messages Service (SMS) Apoteker terhadap kepatuhan terapi hipertensi pasien hemodialisa. Penelitian ini menggunakan pre test dan post test dengan desain kelompok kontrol. Data pasien hemodialisa dikumpulkan selama 4 minggu periode Maret hingga April 2014. Data pasien diperoleh dari rekam medis dan pemberian kuesioner Morisky Medication Adherence Scale Morisky (MMAS). Penelitian ini dilakukan di Poli Hemodialisa RSUD Banjar, Jawa Barat. Analisis statistik untuk mengetahui pengaruh konseling dan Short Messages Service (SMS) terhadap kepatuhan menggunakan uji wilcoxon. Hasil penelitian diperoleh nilai uji kelompok kontrol hasil pre test dan post test (p = 0.206) dan nilai uji kelompok perlakuan hasil pre test dan post test (p = 0.008). Analisis data menunjukkan ada perbedaan tingkat kepatuhan yang signifikan (p = &lt; 0,05) setelah pemberian konseling dengan Short Messages Service (SMS). Kata kunci: Terapi Hipertensi, Hemodialisis, Kepatuhan
EVALUASI PENGARUH PEMBERIAN INFORMASI OBAT OLEH APOTEKER TERHADAP KEPATUHAN PASIEN DIABETES MELITUS TIPE 2 RAWAT JALAN DI RSUD DR. MOEWARDI SURAKARTA Asmini, Puji; Supadmi, Woro; Darmawan, Endang
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Prosiding Seminar Nasional "Perkembangan Terbaru Pemanfaatan Herbal Sebagai Agen Preventif Pada Ter
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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Abstract

ABSTRAK Diabetes Melitus merupakan penyakit kronis gangguan metabolisme yang ditandai dengan kadar gula darah melebihi nilai normal (hiperglikemia) sebagai akibat dari kelainan pada sekresi insulin, kerja insulin, atau keduanya. Prevalensi diabetes diabetes diperkirakan mencapai 2,8% pada tahun 2000, dan diperkirakan terus meningkat. Kepatuhan pasien dan modifikasi gaya hidup memegang peranan penting dalam manajemen diabetes. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian informasi obat oleh apoteker terhadap terhadap peningkatan kepatuhanpasien diabetes mellitus rawat jalan di RSUD Dr Muwardi Kota Surakarta. Penelitian ini dilakukan dengan menggunakan rancangan studi deskriptif observasional dengan pengambilan data secara prospektif, selama  3 bulan dari Juni-Agustus 2014. Responden sebanyak 90 pasien dengan intervensi pemberian informasi obat untuk kelompok 1 dengan leaflet dan oral sebanyak 2kali ulangan, kelompok 2 dengan leaflet sebanyak 2kali ulangan, dan kontrol dengan pemberian informasi di awal kunjungan.Data pasien diperoleh dari rekam medis dan pemberian kuesioner Morisky Medication Adherence Scale (MMAS). Penelitian ini dilakukan di Poliklinik Penyakit Dalam rawat jalan di RSUD Dr Moewardi Surakarta. Mengenai pemberian informasi obat oleh apoteker terhadap kepatuhan pasien diabetes melitus tipe 2 dianalisis menggunakan uji wilcoxon. Hasil penelitian diperoleh nilai uji kelompok kontrol hasil pre test dan posttest (p = 0.001), nilai uji kelompok perlakuan pertama hasil pre test dan post test (p = 0.001), dan nilai uji kelompok perlakuan kedua hasil pre test dan post test (p = 0.000). Analisis data menunjukkan ada perbedaan tingkat kepatuhan yang signifikan (p = &lt; 0,05) setelah pemberian informasi obat dengan leaflet. Kata kunci: Diabetes, Pemberian Informasi Obat, Kepatuhan
PENINGKATAN SISTEM IMUN OLEH KOMBINASI EKSTRAK ETANOL DAUN AWAR – AWAR (Ficus septica burm. F) DAN EKSTRAK ETANOL DAUN KELOR (Moringa oleifera) SEBAGAI KO-KEMOTERAPI KANKER PADA TIKUS PUTIH BETINA GALUR SPRAGUE DAWLEY YANG DIINDUKSI DOKSORUBISIN Anovia, Rizka Angerti; Sari, Dewi Indah; Yazin, Annisa Hardikasari; Ismayani, Ulfa; Darmawan, Endang
Pharmaciana Vol 5, No 2 (2015): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v5i2.2145

Abstract

Use of the drug doxorubicin for cancer therapy causes decreasing of immune system reported. Leaves of Ficus septica Burm. F and leaves of Moringa oleifera as immunostimulatory agents. The purpose of this study, among others, determine the combined effects of ethanol extract of Ficus septica Burm. F leaves and ethanol extract of Moringa oleifera leaves as co-chemotherapy for in female rats Sprague Dawley strain induced doxorubicin. Eighteen Sprague Dawley rats were divided into six treatment groups, normal group was given 0.9% NaCl 10ml / kgBB, positive control group were given by doxorubicin with dose of 4.67 mg / kgBB, single-dose treatment group were each given ethanol extract of Ficus septica Burm F leaves dose of 750 mg / kgBB and ethanol extract of Moringa oleifera leaves 500 mg / kgBB, treatment groups were given by combination of ethanol extract Ficus septica Burm. F leaves and Moringa oleifera leaves with a dose of 1.25 g / kgBB and a dose of 1 , 75 g / kgBB. treatment carried out for 7 days. On days 1 and 4 of each treatment groups except the normal group induced by doxorubicin 4.67 mg / kgBB intramuscularly. Blood sampling performed on day 0 and 7 th for lymphocytes CD3+ and lymphocytes CD8+ test. The results showed that the combination of ethanol extract Ficus septica burm. F leaves and Moringa oleifera leaves dose of 1.25 g / kgBB and the dose of 1.75 g / kgBB can enhanched immune system relative small which was not statistically significantly different (p&gt; 0.05). However, a single dose of ethanol extract Moringa oleifera leaves dose of 500 mg/kg have immunostimulatory activity as lymphocytes CD3+ and lymphocytes CD8+ were more effective than a single dose of ethanol extract of Ficus septica Burm. F leaves dose of 750 mg/kgBB.
HUBUNGAN JUMLAH OBAT YANG DIRESEPKAN DENGAN POTENSIAL PENGGUNAAN OBAT YANG TIDAK TEPAT PADA PASIEN PENYAKIT GINJAL KRONIS DI RS PKU MUHAMMADIYAH YOGYAKARTA Mulyani, Evi; Darmawan, Endang; Mustofa, Mustofa
Pharmaciana Vol 5, No 2 (2015): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.016 KB) | DOI: 10.12928/pharmaciana.v5i2.2063

Abstract

Age-related decrease of physiological function caused some healt problem in elderly, so the problem of treatment in the elderly is also not uncommon found as polypharmacy and Potentially Inappropriate medication (PIMs). The purpose of this study to determine the relationship of the number of drug prescribed with PIMs in elderly patients with CKD in PKU Muhammadiyah Yogyakarta Hospital. PIMs identification by STOPP criteria, while the number of drugs prescribed seen by drugs control books. To assess the patients medical history by interviews and search records. Patient characteristics were analyzed descriptively, correlations between variables were analyzed with chi square test. From 53 samples, PIMs identified 17% and average one sample got one PIMs, while of the 410 drugs that prescribed average patient receiving  8 drugs (&gt; 6). Bivariate test results showed that no significant relationship between the number of drug prescribed with PIMs.
TINGKAT KEPATUHAN ANTIHIPERTENSI DAN PENGONTROLAN TEKANAN DARAH PASIEN RAWAT JALAN RS PKU MUHAMMADIYAH BANTUL, YOGYAKARTA YANG MENDAPATKAN BRIEF COUNSELING-5A DAN SMS MOTIVASIONAL Saputri, Zukhruf Ginanjar; Akrom, Akrom; Darmawan, Endang
Journal of Pharmaceutical Sciences and Community Vol 13, No 2 (2016)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.706 KB) | DOI: 10.24071/jpsc.132189

Abstract

Antihypertensive therapy requires adherence therapy to achieve therapeutic targets such as controlling blood pressure both systolic and diastolic blood pressure. Pharmacists in counseling interventions have been developed in the form of brief Counseling - 5A and SMS reminders. But the relationship between adherence and blood pressure control is still studied and both of them are related to many factors that can affect. The aim of this study was to determine the relationship of the level of compliance antihypertensive therapy  and control of blood pressure (systolic and diastolic) hypertension patients in outpatient PKU Muhammadiyah Bantul, Yogyakarta who get Brief counseling - 5A and SMS motivation.Patients who met the inclusion criteria were divided into 2 groups, the treatment group (n = 30) who received Brief counseling - 5A and SMS motivation, and the control group (n = 30) who received usual care (conventional counseling). Data were collected by interviews using questionnaires compliance of Morisky Medication Adherence Scale (MMAS), the value of blood pressure is taken from the medical record. Correlation between level of adherence and blood pressure control have been analyzed using Fisher test. The results showed the treatment group with categories adherent patients (n=25) was increase in blood pressure control by 36.67% compared with the control group of patients adherent category (n=6) only 6.67% that has control of the blood pressure on post study. In contrast to patient with adherent category, who had uncontrolled blood pressure are 46.67% (treatment group) compared to the control group 13.33% on the post study. Patients categories of non-adherent but is having control of blood pressure showed 3.33% (treatment group) and 26.67% (control group) on the post study. Although the rate of compliance in the treatment group was higher than control group on the second visit, but there was no significant relation between the level of compliance and controlling blood pressure both in control group (p=1.000) and intervention group (p=0.622) on the second visit (post). The rate of compliance and blood pressure control of patients with hypertensive was low. There were many factors such as poor of knowledge, drugs side effect, poly pharmacy, which related with level of compliance. There was no correlation between antihypertensive compliance and blood pressure control in hypertensive patients ambulatory PKU Muhammadiyah Bantul Hospital, who get Brief counseling – 5A and SMS motivation. Therefore it is necessary to do further research to identify factors that influence to improve patients’ awareness in the management of hypertension.