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Monitoring of avian influenza cases based on the detection of viral antigen subtype H5N1 by immunohistochemical technique

Jurnal Ilmu Ternak dan Veteriner Vol 10, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

Monitoring on the cases of Avian Influenza virus was conducted by detecting viral antigen subtype H5N1 usingimmunohistochemical technique. A total of 212 sampels of various avian tissues were collected from the Provinces of East Java(Districts of Madiun, Tulung Agung, Blitar and Kediri), West Java (Districts of Bogor, Bekasi, Cianjur and Sukabumi), Banten(Districts of Pandeglang and Tangerang) and DKI Jakarta. The sampels were collected four times i.e. June 2004, September2004, October 2004 and between January and February 2005. All sampels were stained using immunohistochemical technique.The antigen could be visualized clearly both in the intra-nuclear and intra-cytoplasmic areas of brain, comb, wattle, trachea,lung, heart, breast and thigh muscle, proventriculus, liver, spleen, kidney, intestine and ovary. A number of 39 of 212 cases(18.4%) have been catagorized as positives. The results show that monitoring of HPAI cases conducted in June and September2004 in the Provinces of West Java, Banten and East Java, none of the sampels were positive. However, monitoring of thedisease in September 2004 in the Province of Jakarta showed that AI virus antigen were detected in various organs of chickenfrom Jakarta. Furthermore, monitoring of the disease conducted between October 2004 and February 2005 revealed that AI virusantigen were also detected in chicken not only from Jakarta Provinces but also from Provinces of Banten and West Java. Basedon these results, it is concluded that between June and September 2004, HPAI infection were not found in areas where previousoutbreaks occured in the Provinces of Banten, West Java and East Java. However, the disease was spread in Jakarta Province inSeptember 2004 and subsequently to some districts in the Provinces of Banten and West Java. A part from this, anticipation ofdisease spread to currently AI-free areas should be considered as part of disease monitoring system.Key Words: Avian Influenza, H5N1, Monitoring, Immunohistochemistry, Poultry

Malignant Catarrhal Fever in Indonesia and Its Control Strategy

WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 26, No 3 (2016): SEPTEMBER 2016
Publisher : Indonesian Center for Animal Research and Development

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Abstract

Malignant catarrhal fever (MCF) is an immunoproliferative and lethal disease of many species of the order Artiodactyla (such as families Bovidae, Cervidae and Suidae) caused by a member of the MCF virus (MCFV) group belongs to the genus Macavirus in the subfamily Gammaherpesvirinae. There are two types of MCF i.e. Wildebeest-Associated MCF (WA-MCF) which is caused by Alcelaphine Herpesvirus-1 (AlHV-1) with wildebeest as reservoir animal; and Sheep-Associated MCF (SA-MCF) which is caused by Ovine herpesvirus 2 (OvHV-2) with sheep and goats as reservoir animals. AlHV-1 virus has already been isolated whereas OvHV-2 has not been isolated so that vaccines are not yet available. Both types cannot be differentiated by clinical and pathological findings. This disease was previously diagnosed based on the epidemiological information and clinicopathological findings, but now it can be diagnosed by using molecular biological tests. This paper describes the epidemiology of MCF virus, MCF cases in Indonesia and efforts to control this disease. In Indonesia, SA-MCF cases have been reported almost in all provinces as endemic as well as epidemic nature. Separation of reservoir animal with susceptible species, "producing" a SA-MCF virus free sheep and attempt to develop a recombinant vaccine against SA-MCF is the main control strategy that can be suggested.

The phenotypic detection of surface antigen of B lymphocytes, MHC I and MHC II by immunohistochemical techniques in the lymph nodes of Bali cattle infected with malignant catarrhal fever

Jurnal Ilmu Ternak dan Veteriner Vol 4, No 4 (1999): DECEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

A study on the phenotypic detection of surface antigen of B lymphocytes, MHC I and MHC II in lymph nodes of Bali cattle affected by malignant catarrhal fever (MCF) was conducted by immunohistochemical techniques using avidin-biotin complex peroxidase methods. A number of monoclonal antibodies against surface antigen of B lymphocytes, MHC I and MHC II were used. The results showed that the surface antigens were all detected either in the MCF or in the non-MCF Bali cattle lymph nodes. MHC I were shown predominantly occupied in the lymph nodes of infected cattle. The surface antigen of B cells and MHC II were less found in the infected ones. This indicates that in the immunopathological processes in MCF infected cattle, B cells were not actively involved in producing antibodies, whereas MHC I may contribute the high degree of susceptibility of Bali cattle to MCF.   Key words : MCF, B cells, MHC I, MHC II, Bali cattle, immunohistochemical technique

Phenotypic detection ofT lymphocyte subsets in Bali cattle lymph nodes with malignant catarrhal fever by immunohistochemical techniques

Jurnal Ilmu Ternak dan Veteriner Vol 2, No 2 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

A study was carried out to detect the phenotype ofT cell subsets in six Bali cattle affected with malignant catarrhal fever (MCF). This was performed by means of immunohistochemical technique using avidin-biotin-peroxidase complex methods. Seven monoclonal antibodies against lymphocyte surface antigen were used to detect T cell subsets ie . CD1, CD2, CD4, CD5, CD8, CD45, and WC 1. The results showed that the subsets were all detected either in the MCFor non-MCF infected Bali cattle lymph nodes. However, CD8 was more predominantly occupied in the MCF Bali cattle . This indicated that CD8 was a cytotouic Tlymphocytes and acted as potential mediators for immunopathological process in MCF.   Keywords : Malignant catarrhal fever, Tlymphocyte subsets, Bali cattle, immunohistochemical technique

Pathological features of malignant catarrhal fever in rabbits with a secondary infection of Encephalitozoon cuniculi

Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 1 (1995)
Publisher : Indonesian Animal Sciences Society

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Abstract

Thirty rabbits were used in this study: 22 were infected and eight were used as controls . Fourteen rabbits were inoculated with blood taken from Ongole (Bos indices) cattle or buffalo naturally infected with malignant catarrhal fever (MCF). Another eight rabbits were inoculated with lymphoblastoid cell liras which contain Ovine Herpesvirus-2 (OHV-2), the causal agent of sheep-associated MCF. Various degrees and distributions of histological changes were observed and resembling the lesions developed in MCF. However 17 infected rabbits (77.3%) with non-suppurative meningoencephalitis, eight of them (47.196) were associated with the detection of Encephalhozoon cuniculi histologically . Key words: MCF, Encephalitozoon cuniculi, rabbits

Cases of aspergillosis in Javan Hawk-eagle (Spizaetus bartelst): Isolation of the causative agents and the pathological changes of the diseas

Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 2 (1995)
Publisher : Indonesian Animal Sciences Society

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Abstract

Two cases of aspergillosis in Javan Hawk-eagle (Spizaetus banelsi) from Safari Garden of Indonesia, Cisarua, Bogor, have been reported . Specimens of lungs, airsacs, livers, spleens, kidneys, and intestines were examined mycologically and pathologically . Mycological examination revealed in the isolation of the causative agents from lungs and airsacs, ie . Aspergillus niger from one bird, and Aspergillusfianigarus from the other one . Pathological anatomy examination showed formation of yellowish white miliary nodule lesions, especially in the lungs and airsacs, while histopathological examination showed congestion and granulomatous tissue formation in the lungs, ie . specific lesion of mycotic infection with necrotic zone in the centre, surrounded by lymphocytes, macrophages, fibrin, and fungal hyphae . Pathological changes of the other organs were also described . These findings were the first officially description of aspergillosis cases in these carnivorous birds . Key words: Javan Hawk-eagle, Spizaetus battelsi, aspergillosis, Aspergillus niger, Aspergillus fumigatus

Subclinical malignant catarrhal fever cases in Bali cattle detected in some abattoirs by means of histopathological examination

Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 2 (1995)
Publisher : Indonesian Animal Sciences Society

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Abstract

Surveys were conducted in Mataram (West Nusa Tenggara), Banyuwangi (East Java), Kendari (South East Sulawesi), and Denpasar (Bali) and 413 histopathological specimens were collected from Bali cattle slaughtered in abattoirs . Subclinical malignant catarrhal fever cases were found histologically with the incidence rates as follows : 22 .9% (Mataram, December 1988), 3 .1 % (Mataram, March 1992), 8.8% (Mataram, March 1993), 11 .1% (Banyuwangi, January 1993), 1 .3 % (Kendari, February 1994), and 4 .6% (Denpasar, August 1994) . Totally, from 413 samples examined, the subclinical MCF cases occurred in 36 cattle (8 .6%) . Key words: Malignant catarrhal fever, subclinical, histopathology, Bali cattle, abattoir

The histopathological prevalence of malignant catarrhal fever in swamp buffaloes slaughtered in the private slaughterhouses in Bogor district

Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 3 (1995)
Publisher : Indonesian Animal Sciences Society

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Abstract

Surveys were conducted in five private slaughterhouses in Bogor district to reveal the histopathological prevalence rates of malignant catarrhal fever (MCF) cases in buffaloes . The study was carried out from 1992-1994 in five sub-districts, namely: Ciawi, Caringin, Cijeruk, Megamendung and Cisatua, covering 47 villages . Thirty- two samples (18 .1%) from a total of 177 were histologically being positive for MCF, although the distribution and the severity of lesions were varied amongst them . From those 32 cases examined, lesions observed in the rete mirabile, lung, kidney, liver, urinary bladder, spleen, abomasum, heart, and small intestine were as follows : 84.4%, 77 .3%, 65 .6%, 53 .1%, 28.196, 18 .8%, 9 .4%, 9 .1%, and 3.1% respectively. Keywords : Malignant catarrhal fever, swamp buffalo, prevalence, lession distribution, histopathology

Phenotypic detection ofT lymphocyte subsets in Bali cattle lymph nodes with malignant catarrhal fever by immunohistochemical techniques

Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 2 (1996)
Publisher : Indonesian Animal Sciences Society

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Abstract

A study was carried out to detect the phenotype ofT cell subsets in six Bali cattle affected with malignant catarrhal fever (MCF). This was performed by means of immunohistochemical technique using avidin-biotin-peroxidase complex methods. Seven monoclonal antibodies against lymphocyte surface antigen were used to detect T cell subsets ie . CD1, CD2, CD4, CD5, CD8, CD45, and WC 1. The results showed that the subsets were all detected either in the MCFor non-MCF infected Bali cattle lymph nodes. However, CD8 was more predominantly occupied in the MCF Bali cattle . This indicated that CD8 was a cytotouic Tlymphocytes and acted as potential mediators for immunopathological process in MCF.   Keywords : Malignant catarrhal fever, Tlymphocyte subsets, Bali cattle, immunohistochemical technique

Mareks Disease in Chicken: Clinico-Pathological Aspect and Diagnosis

Indonesian Bulletin of Animal and Veterinary Sciences Vol 12, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

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Abstract

Marek’s is a limphoproliferative and oncogenic disease of poultry caused by a serotype 1 herpesvirus.  Chicken  between 10 and 15 weeks of age are the most susceptible although in some outbreaks it also affects other ages. The causal agent consists of  some  virus  strains  with  different  pathotypes  causing  a  variety  of  clinical  and  pathological manifestations. The  main pathological features are neurological lesion and lymphoma formation in some visceral organs. Marek’s disease is clinically classified as classical, acute, peracute and chronic with neurological disorder and superficial tumor. At necropsy the peripheral nerves were enlarged, discoloured and loss of striation. There were formation of lymphoid tumour in various visceral organs. Microscopically Marek’s produced non suppurative encephalitis/neuritis and pleomorphic tumour. The diagnosis is based on the history of the disease, age of affected chicken, clinical and pathological features. A definitive diagnosis is made by virological and serological tests. Some of the Marek’s cases in Indonesia were discussed in this paper, as well as review on clinico- pathological aspects how to confirm the diagnosis of Marek’s.   Key words. Marek’s Disease, chicken, clinical, pathological, diagnosis