Djoko Said Damardjati
Pusat Penelitian dan Pengembangan Tanaman Pangan Jl. Merdeka No. 147 Bogor

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Cultivation of Thermophilic Bacteria Isolate of alfa-Amylase Production Richana, Nur; Yusuf, Gagan Maulana; Lestari, Puji; Damardjati, Djoko Said
Jurnal Mikrobiologi Indonesia Vol 4, No 2 (1999): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

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Abstract

Cultivation of thermophilic bacteria isolate TVII-6 for a.amylase production. Thermophilic bacteria TVIJ-6 was isolatedand selected from the soil sample taken from Dieng vulcanic. The specific activity of ainylase produced by TVfl-6 was 1624.37U/mg protein. Cassava starch was used as carbon source on enrichment culture and the optimum concentration was 1% withspecific activity 2092.23 U/mg protein. Cultivation in a 2-I bioreactor at pH 7.0 and temperature of 50°C, combine with 250 rpmagitation and 1.0 vvm aeration produced the highest a.amylase. The maximum specific growth rate obtained was 0.147/hours.The relationship between product and substrate was linear Y=0.129 X + 0.024. Efficiency of the substrate to produce amylaseY, was 0.129 g proteinlg substrate. Relationship between the bacterial growth and substrate was Y = 03115 X + 0.013.Efficiency of the substrate for growing the bacteria Y,, was 0.315 g cell/g substrate. Based on the bacterial growth and amylaseproduction, amylase could be considered growth associated, with kinetic parameter of product ratep was 0.37gfgfhr.
Study of the Addition Calcium Ion to alfa-Amylase Activity and Stability from Bacillus stearothermophilus TII 12 ., Rosminik; Richana, Nur; Lestari, Puji; Damardjati, Djoko Said
Jurnal Mikrobiologi Indonesia Vol 6, No 1 (2001): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

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The effcet of storage in liguld or freeze dried condition and calcium ion addition to activity md stability of asmylase from Bacillus stewotkcrmophilhis TlI. have been carried out. The enzyme was stored under several condition:I enzyme was freeze dried, ii enzyme was precipitated by aceton and resoluble in phosphate citrate buffer, iiienzyme was freeze dried after aceton precipitation and reaolibilizstioii in phosphate citrate buffer, Iv enzymeprecipitated by *ceton, resoluble in phosphate Citrate buffer and added by 5 mM calcium ion, and v enzyme wasfreeze dried after aceton precipitation, resolibilization in phosphate citrate buffer and added by 5 mM calcium ion.ReuIts show after nine month storage that the beat enzyme obtained in the addition of calcium ion in the freezedried condition 79.5%.
In Vitro Antioxidant Activity of Stabilized Rice Bran and Its Fraction Damayanti, Evy; Zakaria, Fransiska Rungkat; Syarief, Hidayat; Wijaya, C Hanny; Damardjati, Djoko Said
Jurnal Teknologi Dan Industri Pangan Vol 15, No 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/531

Abstract

Some Researches indicated that oryzanol had antioxidant activity, however, the information about the oryzanol role in the prevention of low density lipoprotein (LDL) and human lymphocyte from oxidation under oxidative stress was still limited. The objective of this study was the investigate the antioxidant activity of oryzanol at concentrations based on rice bran beverage model in preventing LCL and lymphocyte from oxidation. Human plasma were supplemented with the samples of : rice bran oil (RBO), unsaponifiable matter and oryzanol IR-64, oryzanol IR-64 3x and oryzanol standard at the concentrations of 308.3, 22.2, 5.2, 10.4, and 10.4 µg/ml, respectively. Afterward, the human LDL were collected by ultracentrifuge and diluted until a concentration of 200 µg protein/ml was reached. Human LDL isolates were then oxidized with CuSO4 5 µM for measuring antioxidant activity of the sample. The length of incubation, H2O2 concentration, period of sample supplemented into human lymphocyte culture were determined before the antioxidant activity of RBO and its fraction in lymphocyte was measured. The samples used in the lymphocyte were RBO IR-64, unsaponifiable matter IR-64, and oryzanol standard at the concentrations of 133.2 – 2, 132.0 µg/ml, 9.6 – 153.6 µg/ml, and 2.4 – 37.7 µg/ml, consecutively. The result showed that malonaldehyde concentration in human LDL decreased significantly (α = 0.05), 15 – 41% and 39 – 56% compared to the control. The absorbance of living lymphocyte cell in culture was not influenced by the type and concentration of RBO and its fraction. The addition of hydrogen peroxide (H2O2) 3 mM into culture sifnificantly lowered the absorbance as compared to culture without (H2O2). Key words :Oryzanol, oxidative stress, LDL-oxidized, lymphocyte and antioxidant activity.
Analysis of Reducing Sugars on Hydrolysis of Cassava Starch by a Thermostable a-Amylasefrom Bacillus stearothemophilus TII 12 Lestari, Puji; Darwis, Abdul Aziz; Syamsu, Khaswar; Richana, Nur; Damardjati, Djoko Said
Jurnal Mikrobiologi Indonesia Vol 6, No 1 (2001): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

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Abstract

The product of a-amylase hydrolysis was evaluated for their application In sugar syrup Industry. The objectives ofthis experiment were to determine the enzyme production of Bacillus stearothermophilus TH,2 in a five liter bioreactorand to analyze its hydrolysis product on cassava starch using thin layer chromatography and high performance liquidchromatography. The bacterium was cultured in the bloreactor for 48 hours, and then the biomass, enzyme activity,protein and reducing sugar contents in the filtrate were evaluated In the course of cultivation. The strain secreted anextraceiiuiar a-amylase in the optimal condition at pH 6.5, 5O´C, agitation of 300 rpm and aeration of 1.5 vvm for 24hours. The highest activity of a.amylase and reducing sugar content of 1 068.87 U/mI and 4.48 g/l respectively wereobtained after 24 hours incubation. Hydrolysis products by the crude enzyme on cassava starch were evaluated atdifferent Incubation time. In the course of incubation the content of glucose, dextrin, maltose and oligosaccharideswere increasing. After 24 hours the concentration of glucose and maltose reached 51 970 and 10 090 ppm respectively.Based on the enzymatic products, we concluded that thermostabie a.amylsse produced by B. slearothermophilus was aaendo-a-amylase.
Strategi Pengembangan Pasar Domestik Pertanian dalam Menghadapi Persaingan Global Damardjati, Djoko Said
Buletin Iptek Tanaman Pangan Vol 6, No 2 (2011): Desember 2011
Publisher : Puslitbang Tanaman Pangan

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Abstract

Strong and competitive domestic market is a key for facing the world market which is increasingly liberal, open and global. The domestic market structure will change dynamically to adjust the development and to be compatible with the world market. Therefore, the development for better market infrastructure, institutional, human resources, policy and other support facilities should be directed in such a way as to be able to carry out the distribution of goods efficiently that benefits all players, including farmers’ as a producer and consumer. Transformation from farmer producers to farmer suppliers through GAPOKTAN is required. At the distribution and marketing level, there is a need to develop institutional and efficient marketing system through the establishment of the agribusiness terminal (TA) and agribusiness sub-terminal (STA). To build an effective marketing network synergistic cooperation is needed among all agencies and stakeholders, in building and empowering institutional facilities of the existing markets and streamline of critical nodes in the agricultural marketing chains. Traditional markets and modern markets (super market and hypermarket) as the primary goal of marketing of agricultural commodities are objects that need to be fairly regulated. With the very dynamic market developments as demanded by consumers, the traditional market is expected to reorganize themselves into semi-modern interprices in order to remain a good choice for consumers and competitive with modern markets. The development of modern markets both supermarkets and hypermarkets also need to be regulated so that their growth would not to be ‘counter-productive’ with the efforts of building a strong and competitive domestic product market. Competitive domestic market of the local products that compete well with imported products, and the high absorption of the local products is expected to the entry of imported products into the Indonesian market.
In Vitro Antioxidant Activity of Stabilized Rice Bran and Its Fraction Damayanti, Evy; Zakaria, Fransiska Rungkat; Syarief, Hidayat; Wijaya, C Hanny; Damardjati, Djoko Said
Jurnal Teknologi dan Industri Pangan Vol 15, No 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Some Researches indicated that oryzanol had antioxidant activity, however, the information about the oryzanol role in the prevention of low density lipoprotein (LDL) and human lymphocyte from oxidation under oxidative stress was still limited. The objective of this study was the investigate the antioxidant activity of oryzanol at concentrations based on rice bran beverage model in preventing LCL and lymphocyte from oxidation. Human plasma were supplemented with the samples of : rice bran oil (RBO), unsaponifiable matter and oryzanol IR-64, oryzanol IR-64 3x and oryzanol standard at the concentrations of 308.3, 22.2, 5.2, 10.4, and 10.4 �µg/ml, respectively. Afterward, the human LDL were collected by ultracentrifuge and diluted until a concentration of 200 �µg protein/ml was reached. Human LDL isolates were then oxidized with CuSO4 5 �µM for measuring antioxidant activity of the sample. The length of incubation, H2O2 concentration, period of sample supplemented into human lymphocyte culture were determined before the antioxidant activity of RBO and its fraction in lymphocyte was measured. The samples used in the lymphocyte were RBO IR-64, unsaponifiable matter IR-64, and oryzanol standard at the concentrations of 133.2 ? 2, 132.0 �µg/ml, 9.6 ? 153.6 �µg/ml, and 2.4 ? 37.7 �µg/ml, consecutively. The result showed that malonaldehyde concentration in human LDL decreased significantly (? = 0.05), 15 ? 41% and 39 ? 56% compared to the control. The absorbance of living lymphocyte cell in culture was not influenced by the type and concentration of RBO and its fraction. The addition of hydrogen peroxide (H2O2) 3 mM into culture sifnificantly lowered the absorbance as compared to culture without (H2O2). Key words :Oryzanol, oxidative stress, LDL-oxidized, lymphocyte and antioxidant activity.