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Journal : Bioscientia Medicina : Journal of Biomedicine and Translational Research

The Efficacy of Red Ginger Fraction (Zingiber officinale Roscoe var. rubrum) as Insecticidal Aedes aegypti Anwar, Chairil; Syukur, KM Yahya; Dalilah, Dalilah; Salni, Salni; Novrikasari, Novrikasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 2 No 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.40

Abstract

Aedes aegypti is a vector of Dengue Hemoragic Fever. The use of synthetic chemical insecticide is the most common way to control Ae. aegypti. However, since it can cause resistency if it is used in a wide scale with high frequency and continously, it requires an alternative way. Red ginger (Zingiber officinale Roscoe var. rubrum) is one of natural insecticide which is safe to be used to control Ae. aegypti. This research aims was to determine the activity of active fraction of red ginger to mortality of Ae. aegypti. It was a quasi-experiment research with post test only control design. Twenty Ae. aegypti Liverpool strain were the sample of this research with 5 concentration for each and 4 times repetition. The result showed that concentration 0.5%, 1.0%, 1.5%, 2.0% and 2.5% causing mortality percentage of 32.5%, 33.8%, 51.3%, 58.8% and 65.0% respectively. One way Anova test showed p-value < α (0.00 < 0.05). It was concluded that there was a significant difference of various concentration of active fraction n-heksan of red ginger extract to the mortality of Ae. aegypti. The lethal concentration (LC50) was 2.409%. It was suggested to continue this research with reppellant or bio-assay test method.   Key words: Toxicity, extract, fraction, Aedes aegypti, Insecticidal
The Relationship Between Soil Transmitted Helminthes (STH) Infection and Nutritional Status in Students of State Elementary School Number (SDN) 200 Palembang Indonesia Anwar, Chairil; Annisa, Saraswati; Dalilah, Dalilah; Novrikasari, Novrikasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 2 No 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.39

Abstract

Infection of Soil Transmitted Helminthes (STH) is caused by intestinal nematodes where as in its life cycle, soil is needed as the media for the eggs or larvae to mature into effective forms, most commonly happen to children of school age. This infection is still one of the main problems in public health, including Indonesia. STH infection are widely distributed in tropical and subtropical areas. Lack of personal hygiene, poor environmental sanitation and low socioeconomic status are some factors that plays role in increasing the occurrence of the infection. This infection is also one of the causes responsible for malnutrition in children by decreasing appetite and food intake thus ensued adverse consequences such as declining growth pace, impairment of physical health, and weakening cognitive function. This study was conducted to analyze the association of STH infection with nutritional status of SDN 200 students in  Kertapati District, Palembang. This study was an analytic observational research with a cross sectional research design. Samples consist of 107 students chosen using proportional stratified random sampling technique. Data was collected by direct interview using questionnaires, measuring body weight and height to obtain nutritional status which then classified using CDC 2000 growth curve while fecal contamination was examined using Kato Katz and modified Harada Mori methode in the Laboratory of Parasitology Medical Faculty of Universitas Sriwijaya. Data then analyzed using Chi-square test. From 107 students, 27.1% infection of STH was found on 29 students with 6 students (20.7%) infected by A. lumbricoides and 23 students (79.3%) infected by T. trichiura. Proportion of malnutrition status was found at 43.9%. Statistical test showed a significant association between STH infection and nutritional status (p=0.036; OR=3.167; Cl 95%: 1.163-15.237). There was a significant association between STH infection and nutritional status in students of SDN 200 Kelurahan Kemas Rindo Kertapati District Palembang City.
Papaya Leaf (Carica papaya L.) Active Fraction Effective as Bioinsectiside Against Anopheles species (Diptera: Culicidae) Larva Invitro Study Anwar, Chairil; Febriyanto, Febriyanto; Dalilah, Dalilah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 2 No 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.15

Abstract

ABSTRACT   Malaria is one of the spread-disease that become a global public health problem included Indonesia. One of the controlling approach of vector-borne disease is by avoiding direct contact of human and mosquitoes. Natural insecticide is an alternative method that can be used. Carica papaya L. is one of the natural insecticide because contains of alkaloid, flavonoid and saponin. This research was aimed to determine the activity of active fraction. was a quasi-experiment research with post test only group design. This research was conducted in April – June 2017. Extraction and fractination was performed in laboratory of MIPA Biologi of Sriwijaya University. The experiment was performed in laboratory of Entomologi Baturaja. The sample of the research was Anopheles sp. larva instar III and IV. Larvacide test used 6 different concentrations which was 0,125%, 0,25%, 0,5%, 1,0% and 2,0% with aquadest as a control. Anova test and post hoc analysis with SPSS 22 software. The result of efficacy test to Anopheles sp. larva showed that N-hexan was the fraction with highest larvacide ability that kill 95% larva at 2% concentration level. Thin layer chromatography test showed a compound of Terpenoid. Activity test of active fraction N-heksan of papaya leaf with the highest mortality rate 92,5% and the lowest 46,25%. LC50 fraction N-heksan of papaya leaf was high toxicity at 24 hours of observation. Anova test showed there was significant influence and difference of various concentration of active fraction of papaya leaf to the mortality of Anopheles sp. larva with p value < α (0,00<0,05).   Key words             : Anopheles sp. larva, N-hexan fraction of papaya leaf, larvacide