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FERMENTASI DAN KARAKTERISASI ANTIBIOTIK T1O81MY1O-B ASAL Streptoverticillium olivoriticuly Naid, Tadjuddin; Dali, Seniwati
MAJALAH FARMASI DAN FARMAKOLOGI Vol 15, No 1 (2011)
Publisher : Universitas Hasanuddin

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Sejak penemuan Streptomisin oleh Waksman 1994, maka perkembangan penelitian yang mengarah pada penemuan antibiotika baru terus dikembangkan. Hingga saat ini antibiotika masih tetap merupakan obat produk mikroorganisme pilihan dan memegang peranan yang penting dalam pengobatan penyakit infeksi. Penelitian ini bertujuan untuk memproduksi antibio­tika yang diperoleh dari proses fermentasi, memanfaatkan jasa Streptoverticillium olivoriticuly dengan cara pengocokan menggunakan "reciprocal shaker", T1O81MY1O-B diperoleh dengan mengisolasi kaldu fermentasi dengan sentrifugasi dan penyaringan untuk memisahkan miselia dari filtrat. kemudian bagian miselia diekstraksi menggunakan MeOH dan AcOEt. Pemurnian dilakukan dengan kromatografi kolom, dimana aktivitas komponen aktifnya selama proses fermentasi, ekstraksi dan pemurnian dideteksi menggunakan Candida albicans Yu1200 sebagai mikroorganisme uji. T1O81MY1O-B berupa bubuk warna putih, titik leleh 61 – 64° C. Larut dalam etil asetat, etanol, kloroform dan eter, tidak larut dalam air, heksan dan petroleum eter. Larutannya tidak stabil pada larutan alkali. Analisis spektrofotometer ultra violet menunjukkan serapan pada 213 nm (CH3OH) dan 236 nm (NaOH). Sedang analisis spektrofotometer infra merah menunjukkan adanya gugusan hidroksi pada bilangan gelombang 3250 cm, metil pada 2920 cm dan ikatan rangkap -C=CH- pada 1650 cm-1. Aktivitas antimikroorganisme khas pada Trichophyton asteriodes, filtenaria kikuchiana dan Helminthosporium oryzae.
BIOAKTIVITAS ANTIBAKTERI FRAKSI PROTEIN ALGA MERAH Gelidium amansii DARI PERAIRAN CIKOANG KABUPATEN TAKALAR, SULAWESI SELATAN Dali, Seniwati; Natsir, Hasnah; Usman, Hanapi; Ahmad, Ahyar
MAJALAH FARMASI DAN FARMAKOLOGI Vol 15, No 1 (2011)
Publisher : Universitas Hasanuddin

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A research on the ability of protein fraction from red algae Gelidium amansii to inhibit the growth of Salmonella thypi and Staphylococcus aureus has been conducted. Proteins was fractionated from the crude extract by salting out method with  0 – 20 %, 20 – 40 %, 40 – 60 % and 60 – 80 % ammonium sulfat saturation. Proteins was purified by a dialysis method using a selophan membrane. The protein level was determined by Lowry’s method, the highest protein concentration, 5.6 mg/mL was found in the 40 – 60 % fraction  of the red algae. Antibacterial activity test was performed using a layered jelly diffusion method on the  MHA medium. Results indicated that crude extract and the protein fraction of the red algae had antibacterial effect to Salmonella thypi and Staphylococcus aureus. The highest antibacterial activity to Staphylococcus aureus was found in the 0-20% fraction with the inhibition diameter of 14.43 mm after incubated for 24 hours. These research might provide a significant results for the following research on the antibacterial action in molecular  level of protein compounds from marine algae.
Produksi Oligomer Kitosan dari Limbah Udang Windu (Panaeus monodon) Menggunakan Enzim Kitosanase dari Isolat Bakteri Klebsiella sp. Sarni, Sarni; Natsir, Hasnah; Dali, Seniwati
Indo. J. Chem. Res. Vol 3 No 2 (2016): Edisi Bulan Januari (Edition For January)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

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Chitosan is a biopolymer that is the main content of D-glucosamine and several parts of N-acetyl-D-glucosamine bound to β- (1-4) glucoside. Chitosan receive special attention as functional biopolymers for applications in various fields. Chitosan is more effectively absorbed into the human body when it gets converted into chitosan oligomer form. Chitosan oligomer is a mixture of oligomers of D-glucosamine  are formed through a process of severing ties depolymerization of chitosan with β-glycosidic. This study aims to produce chitosan oligomer of waste tiger shrimp (Penaeus monodon) using enzyme kitosanase of bacteria Klebsiella sp. Chitosan oligomer produced by using the enzyme chitosanase at a temperature of 40 °C and pH 8 with the activity of 0.309 U/mL (5,235 U/mg) obtained in the form of a mixture of monomer to octamer, which  soluble in acetic acid 0.25% to 0.5%, having intrinsic viscosity decreases with increasing time of incubation is 0.195 (1 hour incubation); 0.9 (incubation 2 hours) and 0.7 (incubation 3 hours) with molecular weight range of 4103.12 g/mol (incubation 1 hour) ; 1483.48 g/mol (incubation 2 hours) and 1065.79 g/mol (incubation 3 hours).
Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil Firdaus, Firdaus; Dali, Seniwati; Rusman, Hendra J.
Indo. J. Chem. Res. Vol 5 No 1 (2017): Edisi Bulan Juli (Edition For July)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2017.5-fir

Abstract

This research aims to immobilization; characterize the enzyme of immobilized, test the effectiveness of the enzyme of immobilized. This research begins with the immobilization to process of enzyme lipase using activated carbon matrix, enzyme characterization covering of immobile determination of temperature and pH optimum of the enzyme of immobilized, as well as test the stability of work covering immobilized of enzyme the test thermal stability and repeated use. The results showed that the immobile of enzyme work optimally at 50oC of temperature and pH 6.5 with each activity 0.040 U/mL; research results also showed that the immobile of enzyme has higher thermal stability in comparison with the free enzyme: with the relative activity of 57.50% at the time of 45 minutes of exposure and the exposure time at 47.50% at 75-105 minutes and it can be used as many as six times with the relative activity of 52.5% in 6 times of use.
Production and Application of Chitin Deacetylase from Bacillus licheniformis HSA3-1a as Biotermiticide Ischaidar, Ischaidar; Natsir, Hasnah; Dali, Seniwati
Marina Chimica Acta Vol 15, No 1 (2014): Volume 15 No. 1 (2014)
Publisher : Hasanuddin University

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Enzymatic Production of Chitosan from the Waste White Shrimp (Penaeus merguiensis) and Antimicrobial Effect for Durability Against Fishballs Arif, Abdur Rahman; Natsir, Hasnah; Dali, Seniwati
Marina Chimica Acta Vol 15, No 1 (2014): Volume 15 No. 1 (2014)
Publisher : Hasanuddin University

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UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN ARBENAN [Duchesnea indica (Jacks.) Focke] DENGAN METODE DPPH Nuraziza, Nuraziza; Dali, Seniwati; Waris, Risda
Jurnal Ilmiah As-Syifaa Vol 9, No 2 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

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ABSTRACT            Arbenan [Duchesnea indica (Jacks.) Focke] is a plant belonging to Rosaceae family which server as an antioxidant, flavonoids. This research aimed to determine the antioxidant activity of ethanol extract of arbenan leaves using DPPH. The leaves were extracted using maceration method with a sample size of 300 gs dissolved using ethanol 70%, then obtained the viscous extract of 32,49 gs. The arrest of free radicals for each tested sample under the inhibition of DPPH. The tests were conducted at the 4 concentration series of 10, 20, 30, and 40 ppm by using methanol p.a by the addition of 0,5 mL with 3,5 mL DPPH of 30 ppm. Antioxidant activity absorbance was measured by a spectrophotometer at the wavelength of   515 nm and IC50 value was calculated based on the absorbance data. The calculations showed that the Arbenan leaves have a very strong antioxidant activity with IC50 value of 30,20 µg/mL. Keywords :  Antioxidant, Ethanol extract of arbenan leaves [Dunchesnea indica (Jacks) Focke], DPPH.
ISOLASI KITOSAN DARI LIMBAH CANGKANG KEPITING BAKAU (Scylla serrata) DAN APLIKASINYA TERHADAP PENYERAPAN TRIGLISERIDA Dali, Seniwati; Safitri, Nur Ramadhana Dewi; Fawwaz, Muammar
Jurnal Ilmiah As-Syifaa Vol 8, No 2 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

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Chitosan is the result of deacetylation process from chitin which it can be found on Crustacean outer shell such as crabs. Chitosan can bind fat if it was consumed by human. The fat-binding ability of chitosan depends on the deacetylation degree. The research have been made into two phases. The first phase, chitosan was made from crab shell using NaOH. The deacetylation degree from chitosan that was made from the earlier process was analyzed with FTIR. The deacetylation degree result of the research was 59.39% using NaOH 50%. The second phase was the process of adsorbing triglycerides using chitosan in 10, 30, 45, and 60 minutes which was analyzed using spectrophotometer UV-Vis. The result of this research showed that the optimum triglycerides with 0.5, 1, 3 gram of chitosan was 2.99%, 3.14%, and 3.36%. Keywords : Crab shell, Chitosan, Deacetylation degree, Triglycerides
ANALISIS KANDUNGAN ASPARTAM YANG TERDAPAT PADA MINUMAN JAJANAN ANAK SEKOLAH YANG BEREDAR DI MAKASSAR DENGAN METODE HPLC Dali, Seniwati; Kusuma, A. Trihadi; Anar, Afiat Wahyuni
Jurnal Ilmiah As-Syifaa Vol 5, No 2 (2013): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

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This research have done of Aspartame compound in seven kinds of beverage of the student which turn in elementary school with tehe mean that to analyze Aspartame compound in beverage and have purpose to determine the concentration of Aspartame in beverage. As a comparison used the main of Aspartame with purity about 98,38%. The sample is weigh about 10 gram in a flask 50 ml, and then diluted with mobile phase is sodium dihydrogen phosphate and acetonitrile (82.5 : 17.5)ml and then it’s filtered by membrane filter 0,45 um. The result are sonicated and to injection about 20 ml to in colomn with rate of flow 1,2 mk/min and λ 210 nm. The sample is analyzed by HPLC method. The analyzed showed that the average concentration of Aspartame by calculating linear regression equation contained in the sample A. 7.5658 mg/kg, B. 198.3445 mg/kg, C. 257.8844 mg/kg, D. 226.5515 mg/kg, E. 0 mg/kg, F. 45.5389 mg/kg, G. 140.3748 mg/kg which is still below the standard of 600 mg/kg. So, sample E is just not contain of Aspartame. Key words : Aspartame, HPLC, Compound, Analyze
PENGARUH SENYAWA KOFAKTOR DAN STABILITAS TERHADAP AKTIVITAS ENZIM β-1,3-GLUKANASE DARI ISOLAT BAKTERI TERMOFIL Bacillus licheniformis HSA3-1a Dali, Seniwati; Natsir, Hasnah; Gusti, Gusti
Jurnal Ilmiah As-Syifaa Vol 4, No 2 (2012): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

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Study on the effect of compound cofactor and stability to enzyme activity of β-1 ,3-glucanase has been done. This study used bacterial isolates B. licheniformis HSA3-1a isolated from a hot spring Sulili Pinrang as a source of the enzyme β-1 ,3-glucanase. Isolation of enzyme made after bacterial are activated and cultured in fermentation medium, pH 7,0 and temperature of 50 0C for 4 days. The resulting enzyme performed activity assay. Activity of enzyme assays performed by adding the compound cofactor MgCl2, CaCl2, CuCl2, CoCl2, and ZnCl2 at different concentrations (0.25, 0.5, and 1.0) mM. To determine the stability of the enzyme made by varying the incubation time (0, 30, 60, 90, 120 and 180) minutes. The result showed that the cofactors of compounds that can serve as an activator for the enzyme β-1 ,3-glucanase from B. licheniformis HSA3-1a is MgCl2 and CaCl2 at a concentration of 0:25 mM, 0.5 mM and 1.0 mM. Compound cofactor of CaCl2 1 mM is stabilizier of enzyme β-1,3-glukanase because the relative activity of the enzyme remaining 86% of the treatment time for 180 minutes prainkubasi.Key Word : β-1 ,3-glucanase, Bacillus  licheniformis HSA3-1a, cofactor, enzyme activity, stability