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Isolation, Identification and Characterization of the Surface Glycoprotein of Myxobolus koi Insariani, Insariani; Puspasari, Khumaira; Haririyah, Haririyah; Widiyastuti, Emei; Chotimah, Chusnul; Wuryastuti, Hastari
Jurnal Sain Veteriner Vol 30, No 2 (2012)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Myxobolus koi is considered to be one of the pest diseases of  fish quarantine class I, which has to be destroyed if it is found. Technique that is regularly used for diagnosis M. koi, such as histopathological examination, polymerase  chain reaction (PCR) and sequencing always done in dead fishes. The objective of this study was to isolate the protein of M. koi as the initial step for producing antibody that can be used as a material for immunology-based diagnostic method. In the present study. identification of surface protein of M. koi involved three different stages. The first stage was to isolate and identify M. koi from koi fish using wet mount, and histopathologic, PCR and sequencing techniques. In the second stage, purification and isolation of the surface protein of M. koi  was done by Percoll gradient ultracentrifugation technique. Purification using the technique produced 5 different layers on the surface of the tube . Myxobolus koi spores were found in the 2nd, 3rd and 4th layers. The third stage was to identify and characterize the surface protein of M. koi using SDS PAGE. The results of SDS PAGE analysis showed that spores purified from 3 different layers all have the same profiles. Molecular sizes of the surface protein of M. koi that could be isolated in this study were 12 Kd, 16 Kd, 25 Kd, 27 Kd, 40 Kd and 45 Kd. Among them, the molecular sizes of 12 Kd, 25 Kd and 27 Kd could be seen clearly.Keywords: Myxobolus koi, surface glycoprotein, isolation, Percoll gradient centrifugation, SDS PAGE
THE USE OF TABOO WORDS IN PAK DARTO CHARACTER IN TENDANGAN DARI LANGIT MOVIE CHOTIMAH, CHUSNUL
Jurnal Ilmiah Mahasiswa FIB Vol 5, No 10 (2014)
Publisher : Fakultas Ilmu Budaya Universitas Brawijaya

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Abstract

Keywords: taboo words, types, purposes, Tendangan Dari Langit movie, PakDarto.Communication is very important in our life because people can understand each other through communication. Taboo word is one of linguistic phenomena which exist in most languages because every member of society has different way to express his thoughts in language. It is interesting to analyze the various kinds of taboo words which are expressed by men character in the movie Tendangan dari Langit. This study is aimed at describing taboo word phenomena in Tendangan Dari Langit movie. There are two research problems to be solved, those are to find out the types and the purposes of Javanese taboo words used by Pak Darto in Tendangan Dari Langit movie.In this research, the writer used qualitative method with document or content analysis. The writer only analyzed the Javanese taboo words that were spoken by Pak Darto to describe the analysis on types and purposes of taboo words. In analyzing the type of Javanese taboo words, the writer used Wardhaugh’s (2006) theory, while for analyzing the purposes of Javanese taboo words, the writer used Liedlich’s (1973) theory.From the result of analysis, the writer found 10 utterances containing Javanese taboo words by Pak Darto. The writer found that Pak Darto used 5 types of taboo words to express his feelings, they are one’s mother in law, bodily function/human genitals term, death, excretion and creating game/animal. Furthermore, Pak Darto only had 4 purposes in using the taboo words, namely to discredit, to provoke violent confrontation, to an endearment and to provide catharsis.The writer suggests English Department students to conduct the research on taboo language using other theories such as Hughes’s or Holmes’s theory. She also suggests the next researcher to learn more about Javanese taboo language in daily conversation, Javanese poetry or incantation.
Strategi Public Relations Pesantren Sidogiri dalam Membangun Citra Lembaga Pendidikan Islam Chotimah, Chusnul
ISLAMICA: Jurnal Studi Keislaman Vol 7, No 1 (2012): Islamica
Publisher : Program Pascasarjana UIN Sunan Ampel Surabaya

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Abstract

Pesantren has been believed to be a form of indigenous educational system in Indonesia. The history and nature of pesantren have both shown that there is a good relation between pesantren and the society. This will be the focus of the study. It would investigate the notion of how pesantren is related to the wider community, and what are its strategies to keep the community in touch with it? All this will be discussed with referring particularly to the case of pesantren Sidogiri and its already-existing unit called public relation unit. Qualitative in its nature, the paper is phenomenological in its approach. It assumes that generally speaking, any pesantren in Indonesia would –in the beginning- run by accident, and not by design. But as it grows, it becomes as modern as any advanced educational institution would be. Hence, the paper will show that pesantren Sidogiri is aware of the importance of imagebuilding, and that it consciously pursues it through its software and hardware mechanism. The paper will also show that the surrounding community and the alumni are in fact part of the pesantren’s network to build a better image.
Simultaneous Determination Of Metamizole, Thiamin And Pyridoxin In Multicomponent Tablet by RP-HPLC Chotimah, Chusnul
Journal of Food and Pharmaceutical Sciences Vol 2, No 3 (2014): J. Food Pharm. Sci (Sept-December)
Publisher : Gadjah Mada University

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Abstract

The aim of the study was to develop and validate high performance liquid chromatography (HPLC) assay for the simultaneous determination of Metamizole, Thiamine and Pyridoxin in multicomponent tablet dosage form. The experimental procedure involved reversed-phase-HPLC with a Atlantis T3 C18 column (5 μm particle size, 4.6 ID x 150 mm), PIC solution-metanol-acetic acid volumetric  solution (700: 300 : 4, v/v/v) mobile phase, UV detection at 275 nm. The flow rate of the mobile phase was 1.2 mL/min. The method was validated with respect to specificity,  precision, accuracy  and linearity. Due to its simplicity and accuracy, the assay method is suitable for routine analysis of multiomponen tablet formulation.  Key words : metamizole, thiamine, pyridoxin, HPLC
PHLEOMORPHIC ADENOMA (BENIGN MIXED TUMOR) PADA PALATUM MOLLE (LAPORAN KASUS) Supartono, Sigit; Dwiyanto, Dwiyanto; Chotimah, Chusnul
Journal of Dentistry Indonesia Vol 10, No 1 (2003): APRIL
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Phleomorphic adenoma is the most commonly found tumor of the salivary glands. This tumor is usually found in the postero-lateral region of the hard palate. In this case, a phelomorphic adenoma situated in the oropharynx region was reported. The CT-Scan results showed an expansive and infiltrative appearance, suspected to be a malignancy, where wide excision was previously planned to be carried out. During surgery, the mass was found pedunculated in the soft palate. It was then decided to perform an exicision as the choice of therapy.
PERAN PENDIDIK DALAM MEMBANGUN PERADABAN BANGSA MELALUI PENDIDIKAN KARAKTER Chotimah, Chusnul
Jurnal Dinamika Penelitian: Media Komunikasi Penelitian Sosial Keagamaan Vol 16, No 1 (2016)
Publisher : IAIN Tulungagung

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Abstract

The development of civilization depends on the potential of human resourcesis managing. Human resource potential will be maximized when supported by advanced education. In other say, between education and civilization are the two things are directly proportional. Therefore, civilized nations provide dedicated space for education. The more space for the higher education of civilization that will be engraved. Opposite, a civilization that is weak because education does not have a dequate space. The essential values that includes the eighteen character value that discourse is not just a slogan but also requires real implementation and embedded in the character of students, which it could only be integrated through a learning process, a role model and habitus in education through the role of teacher. In genealogy, this article will examine the role of teacher in instilling the value of the character as the support base of civilization, carried out through five major roles namely: transferring, transforming, modeling, transcending, and inspiring. In the process of education, the role fifth isintegrative-mutually inclusive.Keywords: Role of Teacher, Civilization, Character Building
Penatalaksaan Penyakit Gangren Pulpa di RS Bondowoso, Puskesmas Sumbersari, Wuluhan, Pakusari, dan Mayang Tanggal 18 Juni-29 Juli 2013 Kiswaluyo, Kiswaluyo; Adhanti, Rieza; Hermawati, Sisca; Chotimah, Chusnul; W, Dika Fitria
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 11, No 2 (2014)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

Drugs and health storage are components of public health services included dental health service in public health center and hospital. Public health center consider root canal treatment and medicament that used for that treatment. The objective of this study was to know treatment of pulp gangrene in four public health centers and a hospital. This study was descriptive observational   with cross sectional method. Population of this study was patients who diagnosed pulp gangrene in four public health center and a hospital on Juli to Agustus 2013.  This study used probability sampling for taking samples in population continued simple random sampling. The result showed the treatments of pulp gangrene were medication, extraction, and sterilization. Treatment in public health center focused in preventive treatment.
MEMBANGUN BUDAYA ORGANISASI LEMBAGA PENDIDIKAN: PROSES MEMBANGUN NILAI DALAM BUDAYA ORGANISASI UNTUK PENGEMBANGAN LEMBAGA PENDIDIKAN Chotimah, Chusnul
EMPIRISMA: JURNAL PEMIKIRAN DAN KEBUDAYAAN ISLAM Vol 24, No 2 (2015): Reorientasi Pendidikan Islam di Indonesia
Publisher : STAIN Kediri

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Abstract

Culture is dynamic rather than rigid and static. Culture is the implementation of state of mind and normative values of the society. The culture of organization at schools is socialized through internalization of common beliefs and values such as trust, behaviors or attitudes and symbols upon which the norms of collective behaviors are based. Successful internalization of those common values and norms will create secure and conducive working environments among the members, which eventually enhance their organizational commitments and loyalties. In other words, a strong organizational culture will provide the required structures and controls without heavily relying on formal bureaucracies which in many cases can discourage motivation and innovation.Keywords: Budaya Organisasi, Nilai
Uji Daya Hambat Ekstrak Kayu Manis (Cinnamomum Burmannii) Terhadap Pertumbuhan Candida Albicans Pada Gigi Tiruan Akrilik Khatima, Rezky Khusnul; Chotimah, Chusnul; Eva, Andy Fairuz Z.
Jurnal Ilmiah As-Syifaa Vol 9, No 2 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

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Abstract

Denture stomatitis adalah salah satu manifestasi dari kandidosis oral yang disebabkan oleh pemakaian gigi tiruan di malam hari, gigi tiruan yang rusak, ataupun gigi tiruan yang tidak terjaga kebersihannya. Apabila jumlah Candida albicans meningkat melebihi ambang batas normal, maka dalam kurun waktu tertentu Candida albicans tersebut akan menempel pada basis gigi tiruan dan akan menyebabkan infeksi pada pemakai gigi tiruan berupa denture stomatitis yang dapat mengganggu kenyamanan dan kesehatan pemakai gigi tiruan. Kayu manis sebagai bahan alami memiliki efek sebagai anti jamur, anti viral, anti bakteri, dan larvasidal. Tujuan: Untuk mengetahui daya hambat ekstrak kayu manis (Cinnamomum burmanii)  terhadap pertumbuhan Candida albicans yang terdapat pada gigi tiruan akrilik sebagai salah satu alternatif bahan pembersih gigi tiruan.Bahan dan Metode Penelitian: Dilakukan dengan metode eksperimental laboratoris menggunakan desain Post test only control group design. Sampel penelitian adalah apusan jamur Candida albicans yang diambil dari pengguna gigi tiruan akrilik, kemudian dilakukan uji daya hambat  di Laboratorium Mikrobiologi Fakultas Farmasi UMI. Hasil: Berdasarkan uji statistik menunjukan bahwa adanya zona daya hambat ekstrak kayu manis pada semua konsentrasi yang berbeda serta memiliki perbedaan yang tidak signifikan pada masing-masing setiap konsentrasi. Kesimpulan: Daya hambat rata-rata ekstrak kayu manis pada berbagai konsentrasi (20%, 30%, 40%, dan 50%) mempunyai perbedaan diameter rata-rata yang tidak signifikan terhadap pertumbuhan jamur Candida albicans pada gigi tiruan akrilik tetapi memiliki efektifitas yang sama terhadap daya hambat jamur Candida albicans. Ekstrak kayu manis (Cinnamomum burmannii)  pada konsentrasi 20%, 30%, 40%, dan 50% menunjukan adanya perbedaan daya hambat yang lebih efektif dibandingkan dengan obat standar yang biasa digunakan yaitu (Chlorhexidine 0,2%). Daya hambat pada jamur Candida albicans akan semakin meningkat seiring dengan peningkatan kadar konsentrasi dari ekstrak kayu manis (Cinnamomum burmannii).
ANALISIS RASIO KEUANGAN TERHADAP RETURN SAHAM DAN NILAI PERUSAHAAN Chotimah, Chusnul
Jurnal Ilmu dan Riset Akuntansi (JIRA) Vol 2, No 12 (2013)
Publisher : STIESIA

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Abstract

The purpose of this research is to find out the analysis of financial ratio to the company’s stock and value (Tobins’Q). Samples in this research are metal and chemical industry manufacturing companies which are listed in the Indonesia Stock Exchange during the period of 2008–2012 periods by using purposive sampling method. The statistic test method is using the multiple linear regressions analysis in this research. Current Ratio, Total Assets Turn Over, Debt to Equity Ratio, Return on Assets, Return on Equity, Price Earnings Ratio are used as independent variables in this research, stock return and company’s value (Tobins’Q) are used as dependent variables. The result of research shows that current ratio has no significant influence to the stock return and Tobins’Q. Total Assets Turn Over has no significant influence to the stock return but it has significant influence to the Tobins’Q while Debt to Equity Ratio, Return on Assets, Return on Equity, Price Earnings Ratio have significant influence to the stock return and Tobins’Q.Keywords: Financial Ratio, Company Performance, Stock Return, and Tobins’Q.