Chodidjah Chodidjah
Fakultas Kedokteran Universitas Islam Sultan Agung (UNISSULA) Semarang Jl. Raya Kaligawe KM. 4 PO BOX 1054 Semarang Central Java Indonesia, Phone (+6224) 65833584, Fax. (+6224) 6594366.

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Pengaruh pemberian air rebusan meniran (Phyllanthm niruriLinn) terhadap gambaran histopatologi hepar tikus wistar yang terinduksi CCL4 Chodidjah, Chodidjah; Widayati, Eni; Utari, Utari
Jurnal Anatomi Indonesia Vol 1, No 2 (2007)
Publisher : Jurnal Anatomi Indonesia

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Hepatitismerupakan penyakit yang ditularkan secara parenteral dan seringmenjadimenahun dan berakibat fatal.Kerusakan sel hati selain dapat disebabkan oleh virus, juga dapat disebabkan oleh karena obat – obatan.Berbagai tumbuhan obat yang ada di Indonesia diantaranya adalah tumbuhanmeniran diketahuimerupakansenyawa yang dapatmelindungi sel hati dan juga dapatmemperbaiki jaringan hati. Penelitian ini akanmelihatgambaran histopatologi hati pada tikus putih galur wistar yang diberi CCL4 10 % selama 2 hari kemudiandilanjutkan pemberian air rebusanmeniran selama 3minggu.Penelitian inimerupakan penelitian eksperimental dengan rancangan penelitian post test randomized controlgroup design. Digunakan tikus putih galur Wistar, berat badan 150 – 200 gram sebanyak 40 ekor tikus,dikelompokkansecara randomisasimenjadi5 kelompok ,kemudian diberikan perlakuan.Kelompok I tiap tikus diberiCCL4 10%dosis 0,5 cc peroral tiap hari selama dua hari dan dilanjutkan dengan pemberian aqua 1 – 3 cc perhariselama tigaminggu.Kelompok II tiap tikus diberi aqua 1 – 3 cc per hari selama tigaminggu.Kelompok III sampaiVsemua tikus diberi CCL4 10%dosis 0,5 cc tiap tikus peroral selama 2 hari kemudian pada hari ke 3 sampai harike 21 padamasing –masing kelompok diberi perlakuan yaitu pemberian air rebusanmeniran peroral dosis 1 ccperhari , 2 cc perhari dan 3 cc per hari. Pada hari ke 21 semua tikus diambil hatinya dan dibuat sediaanhistopatologi.Hasil kelompok I tampak gambaran histopatologi hati adanya radang kronik, sel limfosit, nekrosis dan perdarahan.Kelompok II gambaran histopatologi hati normal.Kelompok III tampak perlemakan dan nekrosis di sekitar venasentralis.Kelompok IVgambaran histopatologi hati tampak perdarahan .KelompokV gambaran histipatologi hatitampak degenerasi hidropik . Kesimpulan air rebusanmeniran dapatmemperbaiki kerusakan sel hati tikus galurWistar.
Efficacy Assessment of Lemon Peel Aromatherpy Againts Airborne Bacteria Experimental Study in ICU Room of Sultan Agung Islamic Hospital Semarang Sari, Merin Awu; Masfiyah, Masfiyah; Chodidjah, Chodidjah
Sains Medika Vol 4, No 1 (2012): Januari-Juni 2012
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Background: Healthcare associated infections (HAI) are infections acquired in health care centers such as hospital, clinic or other health care sites due to microorganisms. Research conducted by WHO showed 3-10% of all patients treated in hospitals were victims of HAI and 90% were mainly caused by bacteria, the rest by viruses, fungi or protozoa. Lemon peel aromatherapy is one of the essential oils from plants having antimicrobial activity and are beneficial to health reducing bacterial growth by inhibiting the function of cell membranes. This study aimed at evaluating the efficacy of lemon peel aromatheraphy againts airborne bacteria.Design and Method: This experimental study used post test only control groups design. The number of airborne bacteria colonies obtained from ICU room of Sultan Agung Islamic Hospital Semarang treated with lemon peel aromatherapy at the concentration of 100 % and the control group (-).The data were analyzed for normality using Shapiro Wilk followed by independent T-test Result: independent inT-test Independent showed a significant differences in the number of bacterial colonies between the treated groups receiving 100% concentration of lemon peel aromatherapy and control group (-) (p < 0.045).Conclusion: Aromatherapy extracts of lemon peel has effect on reducing the number of airborne bacteria in the ICU of Sultan Agung Islamic Hospital Semarang (Sains Medika, 4(1):71-77).
Nigella Sativa Seed Extract Ointment Increasing the Amount of Healing Fibroblast and Collagen on Balb/C Mice Mastuti, Sri; Hadi, Djoko Tri; Chodidjah, Chodidjah
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 1 (2017): January - June 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Introduction: Untreated cuts in some period of time, caused by various reasons, can be a problem. Alternative therapy used to speed up the healing of thecut is Nigella Sativa (NS). Objective: to investigate the number of fibroblast and collagen by giving 40% and 60% NS seed extract on the cut.Methods: post test only control group design, 48 male balb/c strain rats were divided into 2 large groups, with each cage contained 24 rats. Each groupthen divided to 4. Each group is subdivided into 4. Nigella sativa seed extract ointment with a concentration of 40% (NS-40) and 60% (NS-60) smeared3x daily for 7 days (fibroblasts) and 14 days (collagen). Evaluation derived from preparations using HE staining and Masson’s Trichrome respectively.Results: Mann Whitney analysis shows that the number of fibroblasts and collagen in the CG group, NS-40, and NS- 6 0 Higher than CN, P <0.05.The number of fibroblasts in the NS-40 and NS-60 was not significantly different compared to the CG, as well as CN-40 with CN-60, p> 0.05. Whilethe number of collagen in CN-60 were significantly higher than CN-40, p<0.05.Conclusion : administration of Nigella sativa seed extract ointment with a concentration of 40% and 60% in cuts for 7-14 days increase the number offibroblasts and collagen, resulting in a shorter healing period.
The Trial on the Effectiveness of Honey on White Rats Using Geliate Acetate Method An Experimental Study in Male Wistar Rat Goenarwo, Edijanti; Chodidjah, Chodidjah; Susanto, Heri
Sains Medika Vol 3, No 1 (2011): Januari-Juni 2011
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Background: Pain is the most common complaint of a disease that can be reduced using the analgesic drugs. Besides the pharmacological medication, the traditional medications are available including honey containing flavanoid. The compound can protect the lipid membrane from the damage and inhibit cyclooxygenase, the main path of the synthesis mediator for pain like prostaglandin. This study aims at finding out the efficacy of analgesic of honey using geliate asetate method.Design and methods: In an experimental study using post test only control group design, 25 Wistar rat (80-90 days, BW 180-220 gr) were randomly divided into 3 groups of 5 mice each. Gp1: =aquadest (negative control), Gp2: 0.675 g/BW honey, Gp3: 1.35g honey Gr 4: 2.7 g/kgBW honey and Gr 5 (possitive control): 4.5 mg/ BW of paracetamol. 5 minutes after the treatment, an intraperitoneal injection of acetic acid was given for all groups. Anova followed by post Hoc test was used for data analysis.Result: The honey concentration of 50% (1.35) g was found to have increasing effect of analgesic while the concentration of 25% and 100 % showed a reducing analgesic effect.Conclusion: Honey has been shown to have an analgesic effect in mice with the effective concentration of 50% (1.35 g/kgBW) (Sains Medika, 3(1):48-53).
The Difference in Effectiveness of 70% and 0.5% Chlorine to ReduceThe Germ Number on Stethoscope’s Membrane Experimental research at stethoscope in Baitul Izah Ward of Sultan Agung Islamic Hospital Semarang Pramita, Heny; Saugi Abduh, Saugi Abduh; Chodidjah, Chodidjah
Sains Medika Vol 3, No 1 (2011): Januari-Juni 2011
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

Background: Recently, nosocomial infection (hospital- acquired infections) is estimated to affects more than 1.4 million of in patients in all over the world. Stethoscope is one source of nosocomial infection caused by the continuous contact from one patient to another. 70% alcohol and 0.5% chlorine can be used for disinfection. The purpose of the research is to find out the difference in the effectiveness of 70% alcohol and 0.5% chlorine to reduce the amount of germ on the stethoscope’s membrane.Design and Methods: This was an experimental research with the Post Test Only Control Group Design using 18 stethoscopes divided into 3 groups randomly. Group A was the control group (aquabidest), group B were treated with 70% alcohol treatment, and group C was treated with 0.5% chlorine. The research samples were the stethoscopes used to treat patient in Baitul Izah ward of Islamic Hospital of Sultan Agung Semarang. The data on the germ amount was analyzed using Kruskal-Wallis test. Results: There was a significant difference between aquabidest group and 70% alcohol group (p<0.05) and between aquabidest control group and chlorine group(p>0.05), nevertheless, there was no significant difference between the 70% alcohol group and 0.5% chlorine group ( 0.652). Conclusion: There was no difference in the effectiveness between 70% alcohol and 0.5% chlorine to reduce the number of germ on the stethoscope’s membrane (Sains Medika, 3(1):63-68).
The Effect of Date (Phoenix dactylifera) Juice on Haemoglobin Level An Experimental Study in Iron Supplemented Rats Zen, Ady Try Himawan; Pertiwi, Danis; Chodidjah, Chodidjah
Sains Medika Vol 5, No 1 (2013): January-June 2013
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

There has been more research on the iron supplementation. Date juice has been shown to be rich in iron. It has been reported to increase the hemoglobin level in rats. Few studies has been conducted on the effect of date juice on the hemoglobin level in male white Wistar rats fed low iron diet.This research was conducted to evaluate the effect of (Phoenix dactylifera) juice on haemoglobin level in iron supplemented rats. In this experimental study using post test control group design, 24 male white Wistar rats were divided into 4 groups. G-I served as the control group (standard diet and aquadest). G II was given the low Fe diet and aquadest for 21 d. G-III,IV were given the low fe diet and aquadest plus date juice at the concentration of 50%, 100% respectively. The treatment was given for 14 days. Spectrophotometer was used to assess the haemoglobin level of rats. One way anova followed by Post Hoc LSD was applied for the data analysis. Mean of hemoglobin (g/dl) level for the four groups were 12,03, 7.72, 9.25, 10.35 respectively. Test resulted in p<0.05. Post Hoc LSD test resulted in a significant different between K-I and G-II, G-III, G-IV ;G-II and G-III, G-IV ;G-III and G-IV. In conclusion, date juice increases the haemoglobin level in male white rats fed on the low fe diet.
Typhonium flagelliforme decreases tyrosine kinase and Ki67 expression in mice Chodidjah, Chodidjah; Dharmana, Edi; Susanto, Hardhono; Sarjadi, Sarjadi
Universa Medicina Vol 32, No 3 (2013)
Publisher : Faculty of Medicine, Trisakti University

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Background Worldwide, breast cancer is the most frequent cancer in women after lung cancer. Treatments include surgery, radiation, immunotherapy and chemotherapy, but are not effective. Tyrosine kinase and Ki67 protein are markers of proliferation. Typhonium flagelliforme ethanol extract (TFEE) has been shown to inhibit proliferation of Michigan Cancer Foundation-7 (MCF7) cells in culture. The aim of the present study was to examine the effect of administration of TFEE on tyrosine kinase and Ki67 expression in mice. Methods This experimental study using post test randomized design with control group was conducted in 24 tumor-bearing CH3 mice. They were randomly divided into 4 groups, consisting of one control and 3 treatment groups (TI, T2, T3) treated daily for 30 days with 0.2 ml TFEE at dosages of 200, 400, and 800 mg/kgBW, respectively. On day 31 the tumor tissues were collected and their tyrosine kinase and Ki67 expression were levels assessed using ELISA and immunohistochemical staining, respectively. Tyrosine kinase and Ki67 expression levels were analyzed, respectively using Kruskal Wallis test and one-way Anova followed by Bonferroni post hoc test. Results Mean tyrosine kinase level was highest in the control group, followed by T3, T2 and T1 (p=0.019). Mean level of Ki 67 expression was highest in the control group, followed by T2, T3 and T1 (p=0.000). Conclussions Oral administration of TFEE at a dose of 200 mg/kgBW decreases tyrosine kinase levels and Ki 67 expression.
Typhonium flagelliforme decreases protein expression in murine breast cancer Chodidjah, Chodidjah; Nasihun, Taufiqurrahman; Widayati, Eni
Universa Medicina Vol 33, No 3 (2014)
Publisher : Faculty of Medicine, Trisakti University

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BACKGROUND Breast cancer treatment is still ineffective, having also various side effects. Breast cancer growth is affected by human epidermal growth factor receptor 2 (HER2/neu) and B cell lymphoma 2 (BCL2) expression. In vitro studies on continuous culture of continuous culture of human lymphoblasts (CEMs) showed that Typhonium flagelliforme (TF) increases apoptosis. The objective of this study was to evaluate whether TF syrup (TFS) could decrease HER2/ neu and BCL2 expression as well as breast cancer volume (BCV) in mice. METHODS An experimental post-test only control group design was conducted on 18 C3H mice with breast cancers, randomly allocated to 3 groups of 6. Group 1 received 0.2 ml of distilled water. Group 2 and 3 animals were each given 0.2 ml of 40 mg/ml and 80 mg/ml TFS, respectively. The treatment was given orally once daily for 25 days. Assessment of HER2/neu and BCL2 expression was by immunohistochemistry, whereas BCV was measured by caliper. Anova and LSD were used for data analysis. RESULTS There was a significant difference in HER2/neu and BCL2 expression as well as in BCV among the treatment groups. LSD analysis showed that HER2/neu and BCL2 expression in group 3 (51.60%; 24.60%) was significantly lower than in group 1 (245.40%; 114.40%) as well as group 2 (235.50%; 54.20%) (p=0.000). BCV in group 3 (4392.33 mm3) was significantly greater than BCV in group 1 (253.87 mm3) (p=0.002), but was not significantly different from BCV in group 2 (3667.16 mm3) (p=0.306). CONCLUSION Suplementation with TFS decreases HER2/neu and BCL2 expression. TF appears to be a promising plant demonstarting anti cancer activity.
Formula Jamu Antihipertensi and captopril are equally effective in patients with hypertension Hussaana, Atina; Sarosa, Hadi; Indrayani, Ulfah Dian; Chodidjah, Chodidjah; Widiyanto, Bagas; Pertiwi, Danis
Universa Medicina Vol 35, No 2 (2016)
Publisher : Faculty of Medicine, Trisakti University

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BACKGROUNDHypertension is the main cause of morbidity and mortality associated with cardiovascular diseases. Many herbs/spices appear to have significant effects in favorably modulating high blood pressure. A jamu formulation containing 6 plant extracts, Formula Jamu Antihipertensi (FJA), has been described previously. This research study aimed to evaluate the effect of FJA as antihypertensive agent in mild to moderate hypertensive patients. METHODSThis double-blind experimental study was conducted in 40 hypertensive patients, who were randomized into two groups. The first group was treated with oral administration of 2 g FJA and the second group 25 mg captopril daily for 4 weeks. Systolic and diastolic blood pressure as well as liver and kidney function were followed up every week. Independent- t test and two-way ANOVA were used to analyze the data with a level of significance of 0.05.RESULTSThe results showed that after the treatment, FJA and captopril were capable of significantly decreasing systolic and diastolic blood pressure (p &lt;0.05). The decrease in systolic blood pressure between the two groups was identical (p&gt;0.05), while the decrease in diastolic blood pressure was greater in the FJA group than in the captopril group (p &lt;0.05). CONCLUSIONFrom this study it may be concluded that administration of FJA for 4 weeks is equally effective as captopril in reducing systolic and diastolic blood pressure in patients with mild and moderate hypertension. Thus, natural plants and herbs can be our source of drugs, with fewer side effects and better bioavailability for treatment of hypertension in the future.
TNF-α-Activated MSC-CM Topical Gel Effective in Increasing PDGF Level, Fibroblast Density, and Wound Healing Process Compared to Subcutaneous Injection Combination Kuntardjo, Novalia; Dharmana, Edi; Chodidjah, Chodidjah; Nasihun, Taufiq R; Putra, Agung
Majalah Kedokteran Bandung Vol 51, No 1 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Mesenchymal stem cells (MSCs) are multipotent stromal cells that have the capacity to regenerate tissue damage. However, they have several limitations. MSC-CM as a new approach treatment is widely used to solve the limitation of MSC in wound healing. The aim of this study was to evaluate the effectiveness of TNF-α-activated MSC-CM topical gel compare to topical-subcutaneous injection combination on wound healing acceleration. This study was conducted between April and August 2018 at the Stem Cell and Cancer Research Laboratory (SCCR), Faculty of Medicine, Sultan Agung Islamic University, Semarang. Experimental post-test only control group design was performed by involving 36 animal models randomly divided into six groups; T1, T2 (MSC-CM in topical gel 100 μL; 200 μL); ST1, ST2 (MSC-CM in subcutaneous injection : topical gel = 80 μL:20 μL; 160 μL:40 μL); CT (200 μL medium free TNF-α); CST (PBS in subcutaneous injection : topical gel = 160 μL :40 μL). The measurement of PDGF level on day 3 and 6 was conducted using ELISA assay while the fibroblast density was analyzed by light microcopy. It was found that there was was a significant increase in PDGF and fibroblast density on day 6 in the topical group when compared to the combination group (p<0,05). It is concluded that the MSC-CM topical gel is more effective than combination of topical-subcutaneous injection.Key words: Combination, fibroblast, MSC-CM, PDGF, subcutaneous MSC-CM, topical MSC-CM MSC-CM Topikal yang diaktivasi TNF-α Efektif Dalam Peningkatan Level PDGF, Densitas Fibroblast, dan Mempercepat Penyembuhan Luka dibanding dengan Kombinasi Injeksi SubkutanMesenchymal stem cells (MSCs) merupakan sel stroma multipoten yang memiliki kemampuan untuk meregenerasi kerusakan jaringan. Namun, MSC memiliki beberapa keterbatasan. MSC-CM sebagai terapi pendekatan baru digunakan untuk mengatasi keterbatasan MSC dalam penyembuhan luka. Tujuan penelitian ini adalah menilai efektivitas MSC-CM topikal yang diaktvasi TNF-α dibandingdengan kombinasi topikal-injeksi subkutan pada percepatan penyembuhan luka. Penelitian ini dilaksanakan pada bulan April–Agustus 2018 di Laboratorium Stem Cell and Cancer Research (SCCR), Fakultas Kedokteran, Universitas Islam Sultan Agung, Semarang. Penelitian menggunakan eksperimen laboratorium dengan rancangan post-test only control group, menggunakan 36 tikus galur wistar yang dibagi secara acak menjadi 6 kelompok; T1, T2 (MSC-CM gel topikal 100μL; 200μL); ST1, ST2 (MSC-CM injeksi subkutan : gel topikal = 80 μL:20 μL; 160 μL:40 μL); CT (200 μL medium tanpa TNF-α); CST (PBS injeksi subkutan: gel topikal = 160 μL :40 μL). Pengukuran kadar PDGF pada hari ke-3 dan ke-6 mengunakan ELISA, sedangkan jumlah fibroblas dilihat mengunakan mikroskop cahaya. Hasil penelitian ini menunjukkan peningkatan kadar PDGF dan jumlah fibroblas yang signifikan dihari ke-6 pada MSC-CM gel topikal dibanding dengan kombinasi topical-injeksi subukutan (p <0.05). Simpulan penelitian ini adalah pemberian MSC-CM secara topical lebih efektif dibanding dengan kombinasi topikal-injeksi subkutan.Kata kunci: Fibroblas, konditional medium, MSC-CM kombinasi, MSC-CM topikal, PDGF