Diana Chilmawati
Program Studi Budidaya Perairan, Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Published : 27 Documents
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THE WHITE SPOT SYNDROME VIRUS (WSSV) LOAD IN Dendronereis spp.

JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

The white spot syndrome virus (WSSV), the causative agent of White Spot Syndrome Disease (WSSD), is a major shrimp pathogen in Indonesia. Dendronereis spp. is a ubiquitous Polychaetes and natural food of shrimp raised in brackishwater pond in Indonesia. The objective of this research is to determine the occurrence of WSSV and the viral load in Dendronereis spp. obtained from the shrimp pond. Dendronereis spp. was obtained with PVC (10 cm in diameter) from a traditional shrimp pond in Semarang vicinity. As a comparison, healthy looking Penaeus monodon was also obtained from the same pond. The occurrence of WSSV in Dendronereis spp. was determined with 1-step and nested PCR using primer for WSSV major envelope protein, VP 28. The viral load was counted with 1-step Real Time PCR. The WSSV was detected in Dendronereis spp. with 1-step and nested PCR. The point prevalence of WSSV infection in Dendronereis spp. is 90 %. The viral load ranged from 0 to 1.9 x 104 copy of DNA/µg total DNA. The viral load in Dendronereis is comparable with that of naturally infected and at carrier state P.monodon from the same pond. This is the first report of WSSV load in naturally infected Dendronereis spp.

The Used of Different Culture Medium Effect on The Growth Of Chlorella Sp

SAINTEK PERIKANAN Vol 6, No 1 (2010): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

The effort of aquaculture especially in the brackish and marine water becomes more develope,. That development cannot be separated from seeding phase. To sufficient the needed of seed, it is needed seed aquaculture by giving sufficient and quality life food, because it cannot be replace yet by the imitation food on the early of larvae seedling. One of the life food is Chlorella sp that given to zooplankton variety directly to the fish or shrimp cultivan. Therefore the Walne, Guillard’s f/2, and Erdschreiber culture mediums are suitabel medium used to phytoplankton culture.  The purpose of this research is to know the influence of medium of usage of Walne, Guillard’s f/2, and Erdschreiber on the growth of Chlorella. Also to know the best medium from those three medium on the growth of Chlorella sp the research was Laboratories experimental character. The effort scheme used is completely randomized design 3 treatments and 3 times repetitions,  Those 3 treatments are as follows: A. Walne Medium, B. Guillard’s f/2 Medium, and C. Erdschreiber Medium. The result of the research shows that the used of different culture medium effect on the growth of Chlorella sp, that is significant difference (P<0,05) on the Lag Phase time with best treatment B (0,290 days), great significant difference (P<0,01) on the specific growth rate with best treatment B (0.655). Thus on the maximum density and final density (on Log No. sel/mL) is great significant differences with best treatment B (Guillard’s f/2) each are 7.931 and 6.443. The best medium for Chlorella sp. from those three mediums used in this research is Guillard’s f/2 medium.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          Key Words : medium, culture, growth, Chlorella sp

The Addition of Ammonia Solution with Different Concentrations in the Sperms on the Percentage Ovulation Egg of Abalone, Haliotis asinina

SAINTEK PERIKANAN Vol 5, No 1 (2009): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

The aim of this research was to know the impact of addition of ammoniak in different concentration in the sperm on the percentage of egg ovulation. The research method was carried out by completely randomized design with four treatments and three replicates, respectively. Those treatments were the addition of ammoniac solution with concentration of 0% (treatment A), 0.005% (treatment B), 0.010% (treatment C), and 0.015% (treatment D). The data was analyzed by using ANOVA and Duncan’s Multiple Range Test to know the effect of between the other treatments. The results showed that was the highly significant difference effect (p<0.01) on the egg ovulation of Abalone (H. asinina). The highest egg ovulation rate was obtained in treatment B (94.67% ). The ovulation rate was significantly higher than those in treatments C, D, and A, respectively. According to the results, the best concentration of the ammonia solution to the abalone sperm added was 0.005%. Furthermore, the regression test also showed a positive, linear relationship between spermatozoa motility rate and egg ovulation rate. However, the analysis of orthogonal polymonial provided a ammonia solution with the highest ovulation rate of 0.0057%.  Keywords: Concentration, ammonia solution, ovulation egg, abalone, H. asinina

PERFORMA PERTUMBUHAN HARPATICOIDA Tigriopus sp. DALAM KONDISI KULTUR SEMI MASSAL DENGAN DIET MIKROALGA YANG BERBEDA

Sains Akuakultur Tropis Vol 2, No 2 (2018)
Publisher : Departemen Akuakultur FPIK UNDIP

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Abstract

Tigriopus sp. is a copepod included harpacticoid ordo which is a live food organism for marine fish and shrimp larvae. The lack of information about the appropriate microalgae diet is an obstacle in the semi-mass culture of Tigriopus sp. The purpose of this study was to determine the effect of different microalgae diets and the type of microalgae diet which provides the best growth performance of Tigriopus sp. in semi-mass culture conditions. The experimental design used was a Completely Randomized Design (CRD) with 4 treatments and 4 repetitions namely Tigriopus sp. with a microalgae diet; A. Chlorella vulgaris; B. Tetraselmis chuii; C. Chaetoceros calcitrans; and D. Isochrysis galbana. The results showed that giving C. calcitrans microalgae diet has significant effect (p<0.05) and provides growth performance of Tigriopus sp. Best total density reached 9,75±0,50 inds.mL-1; specific growth rate of the population was 0,114±0,003 inds.day-1 and egg production was 9.26 ± 0.09 eggs.ind.-1 Keywords: Tigriopus sp., growth performance, semi-mass culture, microalgae diet

Aplikasi Teknologi Aquaponic Pada Budidaya Ikan Air Tawar Untuk Optimalisasi Kapasitas Produksi

SAINTEK PERIKANAN Vol 8, No 1 (2012): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

The experiment was conducted to acknowledge the effect of seed to the media using aquaponic system and to  identify the  influence of aquaponic system to the media’s  water quality of Oreochromis  niloticus’s fingerling to increase production capacity. The experiment was conducted at fish farmer’s  fingerling ponds at Muntilan, Magelang (Central Java) for 2 weeks observations.   It used completely  randomized design, were reared at 200,400 and 600 fish density with three replications over to 2 m2 ponds with aquaponic system and 400 densities as a control to non-aquaponic system.  The result of experiment showed that there was an influence of seed’s density to cultivan’s growth that used aquaponic system, especially to total length of fish.  The aquaponic system is also influence the fixing of media water Oreochromis niloticus fingerling, especially the reduction of ammonias (NH3).   Key words: aquaponic, water quality, red tilapia, fingerling

PENGGUNAAN MEDIA KULTUR YANG BERBEDA TERHADAP PERTUMBUHAN Chlorella sp

SAINTEK PERIKANAN Vol 4, No 1 (2008): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

Usaha budidaya ikan di perairan payau dan laut semakin berkembang dan tidak dapat terlepas dari tahap pembenihan. Diperlukan budidaya benih dengan pemberian pakan alami yang cukup dan berkualitas. Pakan alami belum dapat digantikan oleh pakan buatan terutama pada saat awal pemeliharaan larva. Salah satu jenis pakan alami adalah Chlorella sp. yang diberikan kepada jenis zooplankton maupun langsung kepada kultivan ikan atau udang. Media kultur Walne, Gullard’s f/2 dan Erdschreiber merupakan media yang cocok digunakan untuk mengkultur phytoplankton. Tujuan penelitian adalah mengetahui pengaruh penggunaan media Walne, Gullard’s f/2 dan media Erdschreiber terhadap pertumbuhan Chlorella sp dan mengetahui media terbaik dari ketiga media tersebut terhadap pertumbuhan Chorella sp. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan 3 perlakuan dan 3 kali ulangan, yaitu perlakuan A menggunakan Media Walne, perlakuan B menggunakan Media Guillard’s f/2, dan perlakuan C menggunakan Media Erdschreiber. Hasil penelitian menunjukkan bahwa penggunaan media kultur yang berbeda berpengaruh nyata (P<0,05) terhadap waktu lag phase dan berpengaruh sangat nyata (P<0,01) terhadap konstanta pertumbuhan spesifik, puncak populasi dan kepadatan akhir Chlorella sp. Media Guillard f/2 memberikan pertumbuhan Chlorella sp terbaik dengan waktu lag phase tercepat (0,290 hari), konstanta pertumbuhan spesifik terbaik (0,655), puncak populasi tertinggi (8,53 x 107  sel/ml) dan kepadatan akhir tertinggi (2,77 x 106 sel/ml)   Kata Kunci : media, kultur, pertumbuhan, Chlorella sp

Effect of Bakers Yeast, Vitamin B12, and Vitamin C as Nutritional Improvement of Food on the Density Production of Brachionus plicatilis

SAINTEK PERIKANAN Vol 5, No 2 (2010): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

The important factor of seedling production is live food with good quality. One way to improve quqlity of live food, Brachionus plicatilis, to gain a high quality of larvae in seedling production, is through implementing food enrichment. Chlorella sp, which is its live food, can be enriched by combination of baker’s yeast, vitamin B12 and vitamin C, which then given, in order to increase Branchionus plicatilis growth and nutrition content. Purpose of this study is to acknowledge comparative population growth and improvement of Brachionus plicatilis with different kinds of foods enrichment, to understand the right food enrichment composition for Brachionus plicatilis growth. This research are laboratory experimental, with 10 ind/ml early spread density on 5 litres capacity container. The experiment using a Completely Randomize Design with 4 treatment and 3 repetitions. That four treatments included A (Chlorella sp), B (Chlorella sp + baker’s yeast), C (Chlorella sp + baker’s yeast + vitamin B12), D (Chlorella sp + baker’s yeast + vitamin B12 + vitamin C). Result from the research shows that implementation of food enrichment, which using baker’s yeast, vitamin B12 and vitamin C, brings about a great significant differences (p<0,01) in each treatment to population growth. D is the best treatment with specific growth rate of 0,661, maximal density of 530,4 ind/ml and final density of 381,7 ind/ml. Key Words: Elements of Food Enrichment, Population, Brachionus plicatilis

STUDI POLA PERTUMBUHAN DAN KUALITAS SEL Chlorella sp. YANG DIHASILKAN MELALUI TEKNOLOGI PENCUCIAN BIBIT SEL

Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Di dalam kultur massal Chlorella sp. sering terjadi penurunan jumlah sel secara drastis dan lama fase stasioner berselang kurang dari satu hari. Hal  ini diduga karena terjadi hubungan tertutup antara bakteri kontaminan dengan Chlorella sp. di dalam kulturnya. Penelitian ini bertujuan untuk membersihkan Chlorella sp. dari bakteri kontaminan menggunakan teknologi pencucian bibit sel, sehingga dapat memperbaiki pola pertumbuhan dan kualitas sel yang dihasilkan. Metode penelitian ini adalah eksperimen, menggunakan RAL dengan 4 perlakuan dan 4 kali ulangan. Perlakuan itu adalah Chlorella sp. yang dikultur dengan tanpa pencucian bibit sel (A), dengan 1 kali pencucian (B), dengan 2 kali pencucian (C), dan dengan 3 kali pencucian (D). Variabel yang diamati yaitu pola pertumbuhan yang terdiri dari waktu adaptasi, laju pertumbuhan spesifik, lama waktu stasioner, kepadatan sel maksimum, kepadatan akhir kultur, dan kualitas sel dengan kandungan proteinya. Hasil penelitian menunjukkan bahwa pencucian bibit sel berpengaruh nyata (p<0,05) terhadap pola pertumbuhan sel Chlorella sp., terutama pada lama waktu fase stasioner dan nilai kepadatan maksimum sel. Lama waktu fase stasioner pada bibit sel yang mengalami pencucian 3 kali terjadi selama 5,5 hari (D), lebih lama dibandingkan dengan tanpa pencucian bibit sel yaitu selama 2 hari (A) dan kepadatan maksimum sel Chlorella sp. perlakuan D (5,2 X 107 sel/ml), lebih banyak dibandingkan perlakuan A (1,4 X 107sel/ml). Kandungan protein sel Chlorella sp. pada perlakuan A (52,52 %) lebih rendah dibandingkan pada perlakuan D (54,93%). Dari hasil tersebut di atas maka dapat disimpulkan bahwa pencucian bibit sel dapat memperbaiki pola pertumbuhan dan kandungan protein Chlorella sp. pada kulturnya. Mass culture of Chlorella sp. often occurred drastic decrease in the number of cells and a long in the stationary phase which less than one day. It is assumed that due to close correlation of bacterial contaminants on to Chlorella sp. cells in the culture. The aim of this study was cleaned seed cells of Chlorella sp. from the bacterial contaminants by washing cells technology so as to improve the growth pattern and quality of Chlorella cells. The experiment method was employed in this research. There was Completely Randomized Design method with 4 treatments and 4 replicaties, respectively. Those treatments were Chlorella sp. cells cultured with seed cells without washed (A), with one time washed (B), with two times washed (C) and with three times washed (D). Variables observed were growth pattern of Chlorella sp. cells ie: lag phase, specific growth rate, a long time of stationary phase, maximum cells density and the end of culture density, and cells quality with their protein content. The results showed that cells seed washing was significantly effect (p <0.05) on the growth pattern of Chlorella sp. cells, as specialy on the a long time of stationary phase and maximum density. The stationary phase for treatment which washed three times was 5.5 days, longer than the unwashed (2 days). Either on the cell maximum density that higher on treatment D (5.2 X 107 cell/ml) than treatment A (1.4 X 107cell/ml). The protein content also higher on treatment D (54.93%) than treatment A (52.52%). Those could be concluded that cells seed washing to maximalised the growth patterns and protein content of Chlorella sp. cells in culture.

PENGARUH Chlorella sp. DARI HASIL PENCUCIAN BIBIT SEL YANG BERBEDA DALAM FEEDING REGIMES TERHADAP PERTUMBUHAN DAN KELULUSHIDUPAN LARVA KERAPU MACAN (Epinephelus fuscoguttatus)

Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Salah satu kendala dalam budidaya kerapu macan adalah tingkat mortalitas yang tinggi pada fase larva.  Kematian larva diduga karena ukuran dan kandungan nutrisi pakan alami yang diberikan kurang sesuai dengan kebutuhan larva, serta adanya kontaminasi berupa bakteri dan mikroorganisme lain didalam pakan alami yang diberikan. Penelitian ini bertujuan untuk mengkaji pengaruh Chlorella  sp. dari hasil pencucian bibit sel yang berbeda dalam feeding regimes terhadap pertumbuhan dan kelulushidupan larva  kerapu macan (Epinephelus fuscoguttatus) dan mengetahui tingkat pencucian sel Chlorella sp. terbaik yang berpengaruh terhadap pertumbuhan dan kelulushidupan. Metode penelitian adalah eksperimental laboratoris menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan masing-masing 3 kali ulangan.  Perlakuan itu adalah pemberian Chlorella  sp. tanpa pencucian (A), pemberian Chlorella sp. hasil pencucian 1 kali (B), pemberian Chlorella sp. hasil pencucian 2 kali (C), dan pemberian Chlorella sp. hasil pencucian 3 kali (D). Hasil penelitian menunjukkan bahwa pemberian Chlorella  sp. dari hasil pencucian bibit sel kedalam feeding regimes tidak berpengaruh nyata terhadap pertumbuhan panjang relatif, tetapi berbeda nyata terhadap tingkat kelulushidupan. Nilai pertumbuhan panjang relatif pada masing-masing perlakuan adalah 21,48±0,20 %/hari pada perlakuan A,  21,36±0,21 %/hari pada perlakuan B, 21,52±0,24 %/hari pada perlakuan C, dan 21,54±0,20%/hari pada perlakuan D. Nilai terbaik pada variabel kelulushidupan larva kerapu macan ditunjukkan pada perlakuan D dengan tingkat kelulushidupan larva D10 mencapai 7,78±0,19%, dibandingkan pada perlakuan A sebesar 5,22±0,51%. One of the problems of brown-marbled grouper culture were high mortality rate in the larval stage.  The mortality of larval stage assumed due to the size and nutritional content of live food was given less suitable for larvae requirements, as well as the presence of contaminants such as bacteria and other microorganisms in live food. The aims of this research was to know the effect of Chlorella sp. of the results different cell washing seeds in to feeding regimes on the growth and survival rate of brown-marbled grouper  (E. fuscoguttatus) larvae and to determine the best effect of the level of cell washing Chlorella sp. on growth and survival rate. The experiment method was used in this research by completely randomized design (CRD) with four treatments and three replicates respectivaly.  Those treatment were the addition of Chlorella sp. without cells seed washing (A), addition of Chlorella sp. with one times washed (B), addition of Chlorella sp. with two times washed (C), and addition of Chlorella sp. with three times washed (D). The results of research on showed that addition of Chlorella sp. with washed treatment in feeding regimes had no significant effect on the growth of brown-marbled grouper, but the significantly effect on the survival rate.  The growth valeu of relative length in the treatments, respectively were 21.48 ± 0.20 %/day on treatment A, 21.36 ± 0.21 %/day in treatment B , 21.52 ± 0.24 %/day in treatment C, and 21.54 ± 0.20 %/day in the treatment variable D. However, the best result on the survival rate of brown-marbled grouper larvae shown in treatment D with D10 larval survival rate reached 7.78 ± 0.19%, compared to treatment A was 5.22 ± 0.51%.

KARAKTERISASI DAN UJI POSTULAT KOCH BAKTERI GENUS VIBRIO YANG BERASAL DARI MEDIA KULTUR MASSAL MIKROALGA

Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Vibriosis adalah salah satu jenis penyakit yang disebabkan oleh bakteri Vibrio sp. Penyakit ini merupakan salah satu kendala utama yang sering menyerang pembenihan maupun pembesaran udang. Penelitian ini bertujuan mengetahui agensia penyebab vibriosis yang berasal dari media kultur mikroalga dan gejala klinisnya pada udang vaname (Litopenaeus vannamei). Metode yang digunakan penelitian ini adalah metode eksploratif. Metode pengambilan sampel menggunakan metode simple random sampling. Isolasi bakteri menggunakan media Thiosulphate Citrate Bile Salt Agar (TCBSA). Isolat dilakukan seleksi berdasarkan morfologi koloni untuk dilakukan uji postulat koch.  Udang vaname yang digunakan sebagai hewan uji untuk uji postulat koch adalah udang sehat dengan berat 1-1,5 g sebanyak 10 ekor untuk masing – masing isolat dengan ulangan sebanyak 3 kali. Penyuntikan dilakukan pada ruas abdomen kedua dengan kepadatan bakteri 108 CFU/mL dengan dosis 0,1 mL. Pengamatan gejala klinis uji postulat koch dilakukan selama 96 jam. Identifikasi bakteri dilakukan dengan kriteria uji biokimia dan morfologi bakteri. Hasil penelitian didapatkan 21 isolat bakteri. Seleksi bedasarkan morfologi koloni bakteri didapatkan 6 isolat bakteri (TDS10, TDS12, TDS13, TDS15, TDS20, dan TDS9) untuk dilakukan uji postulat koch. Hasil identifikasi bakteri keenam isolat tersebut teridentifikasi sebagai Vibrio harveyi, V. parahaemolyticus, V. alginolyticus, V. fischeri dan V. mimicus yang berpotensi sebagai agensia penyebab vibriosis. Vibriosis was one type of disease caused by genus Vibrio. This disease was one of the major problems in shrimp farming esspecially shrimp hatchery and rearing. Aims of this research to determine the cause of vibriosis derived on culture of microalgae and clinical sign vaname shrimp (Penaeus vannamei) affected by vibriosis. The method in this research used was exploratory research .The sampling method using was simple random sampling method . The isolation of bacteria used Thiosulphate Citrate Bile Salt Agar (TCBSA). Isolate the selection was conducted based on colony morphology for Postulat Koch s test. Shrimp test used vaname for Postulat Koch s test was healthy shrimp with weigh of 1-1.5 as 10 shrimps for every repilcation. The bacterial was injected on second abdominal segment with bacterial density of 108 CFU / mL and 0.1 mL volume. The observations of clinical sign for 96 hour after Postulate Koch’s test. Identification bacteria was carried by biochemical and morphological criterias test. The results were obtained 21 isolates. Selection was done based on bacterial colony morphology of bacterial isolates was obtained 6 isaolates (TDS10, TDS12, TDS13, TDS15, TDS20 and TDS9) these isolates was continue for postulates kochs test. The results of identification six bacterial isolates was identified as Vibrio harveyi, V. parahaemolyticus, V. alginolyticus, V. fischeri and V. mimicus as an agent potentially cause vibriosis