Siti Rasminah Chailani
Jurusan Hama dan Penyakit Tumbuhan, Universitas Brawijaya Malang

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Evaluation of Sucrose for In Vitro Germination and Growth of the Entomopathogenic Fungus Beauveria bassiana (BALSAMO) VUILLEMIN and Paecilomyces sp. (DEUTEROMYCETES, MONILIALES) Afandhi, Aminudin; Chailani, Siti Rasminah; Agustiawati, Agustiawati
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.233 KB)

Abstract

AbstractIn order to examine some possible effects of granulated sugar and sucrose in B. bassiana conidiasuspension, we examined the effects of sucrose on germination dan growth of Beauveria bassiana and Paecilomyces sp. in vitro. We hoped to provide some information relevant to mass production methods for B. bassiana and Paecilomyces sp. The conidia germination and growth of B. bassiana and Paecilomyces sp. were increased by addition of sucrose in medium. The addition sucrose maybe a useful carbohydrate source for use in mass production and carrier ingredient into conidiasuspension as bioinsecticide.Keywords: Beauveria bassiana, Paecilomyces sp., conidia germination, mycelial growth, sucrose
ISOLATION AND PHENOTYPIC CHARACTERISATION OF MORPHOLOGY IN FUNGUS Beauveria bassiana (Balsamo) Vuillemin COLONY NATURALLY FROM LEAF SURFACE, SOIL, AND INSECT AS HOST IN TOMATO PLANTATION Affandi, Aminuddin; Chailani, Siti Rasminah; Mimbar, Saubari M.; Wiroatmodjo, Boedijono
AGRIVITA, Journal of Agricultural Science Vol 34, No 3 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i3.229

Abstract

Virulence of fungus Beauveria bassiana as pathogen on insect can be predicted according to phenotypic character in colony morphology. First of all, isolates of three different habitats were taken. Furthermore, their phenotypic characters was further characterised in B. bassiana colony and the colony growth rate was also monitored. The three natural habitats comprised insects, soil in tomato plantation and the surface of tomato leaves. The result showed that B bassiana isolate managed to be isolated from the three natural habitats involving larva Spodoptera litura and Helicoverpa armigera, the soil, and surface of tomato leaves. B bassiana isolate of insect habitat represented phenotype in colony morphology with curved character, while isolate of the leaves? surface and soil indicated phenotype in colony morphology with raised and bowl-shaped character. In addition to the colony characters, varied growth rate was also indicated by isolate from each habitat. Isolate of insect with curved colony character represented higher growth rate than that of the leaves? surface or the soil with raised and bowl-shaped colony character. The colony with curved character represented phenotypic character of isolate B bassiana with high growth rate. Thus, B. bassiana isolate with the curved colony of insect served as an isolate which was expected to be virulent as controlling agent to biological elements.       Keywords: B. bassiana isolate, phenotypic characters in colony morphology, colony growth rate, habitat of fungus