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Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNA ALIMUDDIN, .; ANDRIYANI, IRMA; JUNIOR, MUHAMMAD ZAIRIN; ARFAH, HARTON; OCTAVERA, ANNA; CARMAN, ODANG; YOSHIZAKI, GORO
HAYATI Journal of Biosciences Vol 18, No 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.232 KB) | DOI: 10.4308/hjb.18.3.135

Abstract

Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.
Fin Type Variation of Lionhead Strain Goldfish (Carassius auratus) Offspring Syaifudin, M.; Carman, Odang; Sumantadinata, K.
Jurnal Akuakultur Indonesia Vol 3, No 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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 Lionhead strain goldfish (Carassius auratus) inheritance produce many variations in phenotype qualitative traits of their offspring that is not common with the parents. Lionhead is an ornamental freshwater fish, they do not have a dorsal fin, but it is a beauty finfish, is popular to the people and have a high economic value. Of the 846 offspring of lionhead is produced in this experiment, and 57,7% of them have dorsal fin (42,3% normal), 13,1% of them have anal fin which did not similar with their parents, 58,6% caudal fin of them did not have similar to their parents. It might be caused by incompletely segregation in meiosis and many gen responsible to certain phenotype trait. Another abnormalities such as no anal fin, blindness, stumped and no pigmen in their gill lamella also occurred. Key words: Inheritance, fin, phenotype, abnormality   ABSTRAK Ikan maskoki strain lionhead menghasilkan keturunan dengan fenotip yang sangat bervariasi dan berbeda dengan induknya. Ikan ini merupakan ikan hias air tawar yang tidak memiliki sirip punggung namun tetap memiliki keindahan, sehingga menjadi begitu populer di masyarakat dan memiliki nilai ekonomi yang tinggi. Sebanyak 846 keturunan (anakan) telah dihasilkan dalam percobaan ini, dan sebanyak 57,7% dari populasi tersebut memiliki sirip punggung (berarti 42,3% merupakan keturunan normal), 13,1% memiliki sirip dubur yang tidak mirip dengan induknya. Variasi keturunan ikan maskoki strain lionhead ini disebabkan oleh segregasi yang tidak sempurna dalam proses meiosis dan banyaknya gen yang terlibat dalam pembentukan penotip tertentu. Abnormalitas lainnya juga terjadi pada keturunan ikan maskoki strain lionhead ini, seperti tidak adanya sirip dubur, mata buta, tubuh pendek dan tidak adanya pigmen pada lemela insang Kata kunci: Keturunan, sirip, fenotip, abnormalitas  
Feminization of Betta Fish (Betta splendens Regan) Through Embryo Immersion in Estradiol-17β Hormone Solution at the Dosages of 400 µg/I for 6, 12, 18 and 24 Hours Purwati, S.; Carman, Odang; Junior, M. Zairin
Jurnal Akuakultur Indonesia Vol 3, No 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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The experiment was carried out to study the effect of embryo immersion in estradiol-17β hormone solution on sex ratio, hatching rate and survival rate of betta fish. Eyed embryo were immersed in 400 µg/I estradiol-17β for 0 (control), 6, 12, 18 and 24 hours, and then the larva were reared until reaching three month old. Immersion in estradiol-17β hormone increased percentage in female, and the highest value was achieved at 12 hours of immersion. Increasing treatment duration to 24 hours decreased the survival rate of betta fish. The lowest value of hatching rate was found at 24 hours of immersion. Key words: Feminization, betta fish, betta splendens, sex ratio, survival rate and hatching rate
Pair replacement on the spawning success of broodstock Seahorse (Hippocampus barbouri) Syafiuddin, .; Junior, M. Zairin; Jusadi, Dedi; Carman, Odang; Affandi, Ridwan
Jurnal Akuakultur Indonesia Vol 10, No 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

Seahorse, (Hippocampus barbouri) is one of marine living resources having high commercial values and has commonly been traded especially as live ornamental aquarium fish, raw material of traditional medicine and as souvenirs. This expriment was conducted to determine the succces of spawning rate by replacing the broodstock pair of seahorse. This study was done experimentally with treatment of replacement of broodstock pair after spawning under control condition. The experiment was designed to apply completely randomize design by using the following treatments: Treatment A, without replacement neither male nor female. Treatment B, spawned female broodstock  was being mated with her unpaired male broodstock.  Treatment C, a male broodstock that still brood was being mated with his unpaired female broodstock.  Treatment D, a spawned male broodstock that has released larva was being mated with his unpaired female broodstock.  Results showed that under control condition the replacement of broodstock pairs of seahorse had significantly influenced the spawning interval, number of eggs released and number of juveniles produced (P0,05).  It can be concluded that seahorse is not monogamous, either male or female after being spawned may accept other pair for the next spawning. Key words: pair replacement, broodstock, success spawning, Hippocampus barbouri   ABSTRAK Kuda laut, (Hippocampus barbouri) merupakan salah satu sumberdaya hayati laut yang memiliki nilai komersial dan telah banyak diperdagangkan terutama sebagai ikan hias, bahan baku obat tradisional dan juga sebagai suvenir. Penelitian ini dilakukan dengan tujuan untuk mengkaji tingkat keberhasilan pemijahan dengan penggantian pasangan induk kuda laut pada wadah budidaya. Percobaan ini dilakukan secara ekperimental dengan perlakuan penggantian pasangan induk setelah pemijahan dalam wadah budidaya. Percobaan dirancang dengan menggunakan rancangan acak lengkap (RAL) dengan perlakuan sebagai berikut: Perlakuan  A, pemijahan   sepasang  induk kuda laut (tanpa pergantian). Perlakuan B, pemijahan induk betina yang telah memijah dengan induk jantan bukan pasangannya. Perlakuan C, pemijahan  induk  jantan  yang telah memijah (mengerami telur) dengan induk betina bukan pasangannya. Perlakuan D, pemijahan induk jantan yang telah melahirkan dengan induk betina bukan pasangannya. Hasil penelitian menunjukan bahwa penggantian pasangan induk pada wadah budidaya sangat berpengaruh terhadap interval pemijahan, jumlah telur yang dikeluarkan dan jumlah juwana yang dihasilkan (P0,05). Dari hasil penelitian ini disimpulkan bahwa kuda laut, tidak bersifat monogami, artinya baik jantan maupun betina setelah memijah dapat menerima pasangan lain untuk pemijahan berikutnya. Kata kunci: induk, keberhasilan pemijahan, pergantian pasangan, Hippocampus barbouri
The Caryotipe of Endemic Ornamental Fish Celebes Rainbow (Telmatherina ladigesi) from Maros River, South Sulawesi Andriani, I.; Sugiri, N.; Carman, Odang; Sjafei, D.S.
Jurnal Akuakultur Indonesia Vol 3, No 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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The objective of this research was to find the caryotype of the endemic ornamental fish, Celebes rainbow (Telmatherina ladigesi) from Maros River South Sulawesi, Indonesia. The experimental fish were caught from the branches of Maros River, those are Bantimurung River, Pattunuangasue River and Makkatoang River. Tissue collection and slide preparation were carried out using the solid tissue technique with slight modification. The amount of chromosome pairs found from those three rivers (three population) were 2n = 48. These chromosomes were divided into 13 pairs big chromosomes (>1 urn) and 11 pairs small chromosomes (I u,m) dan 11 pasang kromosom kecil (
Effect of Triiodothyronine in Different Dosages on Growth and Survival Rate of Giant Gouramy (Osphronemus gouramy Lac.) Herviani, I.; Junior, M. Zairin; Carman, Odang
Jurnal Akuakultur Indonesia Vol 2, No 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

This experiment was conducted to study the effect of giant gouramy larval immersion in triiodothyronine (T3) hormone solution on their growth and survival rate. One-day old larvae were treated with different concentrations of triiodothyronine (T3) i.e. 0; 0,001; 0,01; 0,1 and 1 ppm by immersion method for 24 hours. During eight weeks rearing period, larvae were fed to satiation with Tubifex three times daily. The media were aerated and changed 10-30% daily. At the end of experiment, there was no significant difference in yolk sac absorption between treated larvae and control. The highest dose of 1 ppm T3 resulted significant decreased in total length, average weight and survival rate of larvae. Key Word : Giant gouramy, Osphronemus gouramy, triiodothyronine, immersion dose, growth, survival rate.   ABSTRACT This experiment was conducted to study the effect of giant gouramy larval immersion in triiodothyronine (T3) hormone solution on their growth and survival rate. One-day old larvae were treated with different concentrations of triiodothyronine (T3) i.e. 0; 0,001; 0,01; 0,1 and 1 ppm by immersion method for 24 hours. During eight weeks rearing period, larvae were fed to satiation with Tubifex three times daily. The media were aerated and changed 10-30% daily. At the end of experiment, there was no significant difference in yolk sac absorption between treated larvae and control. The highest dose of 1 ppm T3 resulted significant decreased in total length, average weight and survival rate of larvae. Key Word : Giant gouramy, Osphronemus gouramy, triiodothyronine, immersion dose, growth, survival rate.
Effectiveness of B-actin promoter on driving target gene expression in common carp transgenesis Hidayani, Andi Aliah; Carman, Odang; Alimuddin, .
Jurnal Akuakultur Indonesia Vol 10, No 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

Promoter in transgene construct plays an important role on regulating of transgene expression level in transgenic fish. In fish transgenesis, researcher convinced that use all-fish gene construct is safety and prospective. This study was performed to compare effectiveness b-actin promoter, - the promoter which has ubiquitous, constitutive, housekeeping characteristics, from common carp (homologous) and from tilapia and medaka b-actin promoters (heterologous) in driving of green fluorescent protein (GFP) expression as a model of target gene on common carp transgenesis. These gene constructs were separately microinjected into cytoplasm of 60 one-cell-stage common carp embryos. The results suggested that 70% survival rate at embryo stage and 45% hatching rate values showed that the microinjection was performed successfully. Percentage of embryos expressing GFP gene were slightly higher when injected using common carp and medaka promoters than those of using tilapia promoter. Percentage of larvae expressing GFP using common carp promoter was similar with medaka promoter. Furthermore, GFP expression using common carp b-actin promoter could be detected at one-week-old larvae, while GFP expressing using medaka b-actin promoter was lasted at 2-day-old larvae. The results demonstrated that homologous promoter more effective in driving of a target gene expression than that of heterologous promoter.  Key words: homologous promoter, GFP, transgenesis, common carp   ABSTRAK Promoter dalam konstruksi transgen berperan penting dalam pengaturan tingkat ekspresi transgen pada ikan transgenik. Dalam transgenesis ikan, peneliti meyakini bahwa penggunaan konstruksi gen "all-fish" adalah aman dan prospektif.  Penelitian ini dilakukan untuk membandingkan efektivitas promoter β-aktin, - promoter yang memiliki ciri ubiquitous, constitutive, dan housekeeping, dari ikan dari ikan mas (homolog) dan ikan nila dan ikan medaka (heterolog) dalam mengendalikan ekspresi gen GFP sebagai model gen pada transgenesis ikan mas. Setiap  konstruksi gen tersebut diinjeksikan secara terpisah ke sitoplasma embrio ikan mas fase 1 sel sebanyak 60 embrio. Hasil penelitian dengan kelangsungan hidup embrio 70% dan derajat penetasan 45% menunjukkan bahwa kegiatan mikroinjeksi berhasil dengan baik.  Persentase embrio mengekspresikan gen GFP yang diinjeksi konstruksi gen dengan promoter β-aktin ikan mas dan ikan medaka sedikit lebih tinggi dibandingkan dengan yang menggunakan promoter β-aktin ikan nila.  Selanjutnya, ekspresi gen GFP yang dikendalikan oleh promoter β-aktin ikan mas dapat dideteksi pada larva berumur 1 minggu, sedangkan ekspresi GFP dengan promoter β-aktin ikan medaka hanya bisa terdeteksi hingga larva berumur 2 hari.  Hasil penelitian menunjukkan bahwa promoter homolog adalah lebih efektif dalam mengatur ekspresi gen target dibandingkan dengan promoter heterolog. Kata kunci: promoter homolog, GFP, transgenesis, ikan mas
The use of zeolite, active carbon, and clove oil in closed transportation of giant freshwater prawn juvenile Anandasari, Rahma Vida; Supriyono, Eddy; Carman, Odang; Adiyana, Kukuh
Jurnal Akuakultur Indonesia Vol 14, No 1 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

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ABSTRACT The objective of this study was to determine the effect of zeolite, active carbon, and clove oil on water quality (dissolved oxygen/DO, total ammonia nitrogen/TAN, temperature) and biological quality (glucose concentration, total protein, survival/SR) of giant freshwater prawn juvenile Macrobrachium rosenbergii in closed transportation system. The study was conducted in laboratory scale with a completely randomized design. The biota used was juvenile giant prawn with an average weight 0.407 ± 0.005 g/ind. The type and dose of additive used were A (20 g/L zeolite + 10 g/L active carbon + 14 µL/L clove oil), B (20 g/L zeolite + 10 g/L active carbon + 9.33 µL/L clove oil), C (20 g/L zeolite + 10 g/L active carbon + 4.67 µL/L clove oil), D (20 g/L zeolite + 10 g/L active carbon + 1.87 µL/L clove oil), K+ (20 g/L zeolite + 10 g/L active carbon), and K- (without material addition). The glucose concentration of treatment B and C significantly different with treatment A, D, K+, K-. Total protein of treatment A, B, C and K+ significantly different with treatment K-. DO, TAN, and temperature of the transportation media were still in the suitable concentration for living of giant prawn. The highest survival of the prawn was observed in group C. The result showed the combination of 20 g/L zeolite + 10 g/L active carbon + 4.67 µL/L clove oil in the water is suitable for closed transportation system for juvenile giant freshwater prawn. Keywords: glucose concentration, total protein, DO, TAN, temperature  ABSTRAK Penelitian ini bertujuan untuk menentukan pengaruh pemberian zeolit, karbon aktif, dan minyak cengkeh terhadap kualitas air (dissolved oxygen/DO, total ammonia nitrogen/TAN, suhu) dan kualitas biologi (konsentrasi glukosa, total protein, tingkat kelangsungan hidup/TKH) benih udang galah Macrobrachium rosenbergii pada sistem transportasi tertutup. Penelitian dilakukan pada skala laboratorium dengan rancangan acak lengkap. Biota yang digunakan yaitu benih udang galah dengan bobot rata-rata 0,407±0,005 g/ekor. Dosis bahan tambahan yang digunakan adalah: A (20 g/L zeolit + 10 g/L karbon aktif + 14 µL/L minyak cengkeh), B (20 g/L zeolit + 10 g/L karbon aktif + 9,33 µL/L minyak cengkeh), C (20 g/L zeolit + 10 g/L karbon aktif + 4,67 µL/L minyak cengkeh), D (20 g/L zeolit + 10 g/L karbon aktif + 1,87 µL/L minyak cengkeh), K+ (20 g/L zeolit + 10 g/L karbon aktif), dan K- (tanpa bahan tambahan). Konsentrasi glukosa perlakuan B dan C berbeda nyata dengan perlakuan A, D, K+, K-. Total protein perlakuan A, B, C, D, dan K+ berbeda nyata dengan perlakuan K-. DO, TAN dan suhu media transportasi masih sesuai dengan kehidupan udang galah. Tingkat kelangsungan hidup transportasi tertinggi yaitu pada perlakuan C. Hasil menunjukkan bahwa kombinasi 20 g/L zeolit + 10 g/L karbon aktif + 4,67 µL/L minyak cengkeh adalah perlakuan yang sesuai untuk transportasi tertutup benih udang galah. Kata kunci: konsentrasi glukosa, total protein, DO, TAN, suhu
Induced maturation of eel weighed 100–150 gram with PMSG, antidopamine, and 17α-methyltestosterone Aryani, Nadia Mega; Sudrajat, Agus Oman; Carman, Odang
Jurnal Akuakultur Indonesia Vol 14, No 2 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

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ABSTRACT Marketed eel Anguilla bicolor bicolor is commonly produced from larvae rearing activity whose broodstocks and larvae are caught from the nature. Supply of eel broodstock is restricted by its life cycle and uncertain size variation of mature male and female. This study was aimed to evaluate the effect of hormonal induction through injection to enhance masculinization and gonadal maturation of eel at the weight of 100–150 g. The experiment used completely random design with the use of combination pregnant mare serum gonadotropin  (PMSG), antidopamine (AD), dan 17α-methyltestosterone (MT), which were (10 IU/kg PMSG + 0,01 mg/kg AD), P2 (20 IU/kg PMSG + 0,01 mg/kg AD), P3 (10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), P4 (20 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), and P5 (control; without hormonal treatment). The result showed that an increasing of fish length along with fish weight were performed by treatment P4 and P3. The highest gonadosomatic index value was obtained by treatment P3 (1,3030±0,24262). Based on gonadal histology analysis, 2nd phase of spermatogonia development was found in P3 in week-8. The highest testosterone level was obtained by treatment P3, followed by P4, P2, and P1 in week-4. Combination of 10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT could enhance masculinization and gonadal maturation of eel in eight weeks of rearing period. Keywords: gonadal maturation, Anguilla bicolor bicolor, PMSG, AD, MT  ABSTRAK Ikan sidat Anguilla bicolor bicolor yang dipasarkan pada umumnya merupakan hasil usaha pembesaran yang benih dan induknya masih diperoleh dari alam. Penyediaan induk ikan sidat terkendala dengan siklus hidup dan variasi perbedaan ukuran induk ikan sidat jantan dan betina matang gonad yang belum pasti. Penelitian ini bertujuan untuk mengkaji peran induksi hormonal yang disuntikkan pada ikan sidat dalam mempercepat proses pematangan gonad ikan sidat ukuran 100–150 g. Metode penelitian yang digunakan adalah metode eksperimen rancangan acak lengkap dengan kombinasi pregnant mare serum gonadotropin (PMSG), antidopamin (AD), dan  17α-metiltestosteron (MT) sebagai berikut P1 (10 IU/kg PMSG + 0,01 mg/kg AD), P2 (20 IU/kg PMSG + 0,01 mg/kg AD), P3 (10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), P4 (20 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), dan P5 (kontrol; tanpa perlakuan hormon). Hasil penelitian menunjukkan peningkatan pertambahan panjang seiring dengan pertambahan bobot diperoleh dari perlakuan P4 dan P3. Indeks gonadosomatik tertinggi diperoleh dari perlakuan P3 (1,3030±0,24262). Hasil histologi gonad ditemukan perkembangan spermatogonia fase 2 pada P3 di minggu kedelapan. Konsentrasi testosteron tertinggi didapat dari perlakuan P3 kemudian diikuti P4, P2, dan P1 pada minggu keempat. Kombinasi hormon 10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT dapat mempercepat pematangan gonad dan pertumbuhan pada ikan sidat selama delapan minggu pemeliharaan. Kata kunci: pematangan gonad, Anguilla bicolor bicolor, PMSG, AD, MT 
Testicular cell transplantation of neon tetra Paracheirodon innesi into common carp fry Alimuddin, ,; Carman, Odang; Wulandari, Sri Setyo
Jurnal Akuakultur Indonesia Vol 12, No 2 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

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ABSTRACT Neon tetra Paracheirodon innesi is an ornamental fish that have high export value. However, production is still relatively low due to low fecundity (approximately 180 eggs/female). Technology of testicular cell transplantation of neon tetra as donor to common carp as recipient fish which have high fecundity provides a promising way to overcome problem of neon tetra production. This research was performed to determine the optimum age of common carp fry that is able to receive donor cells and allow high success of transplantation. In this research, the testes of neon tetra fish were dissociated by 0.5% trypsin solution. The testicular cells were labeled with PKH-26 fluorescent dye, and then transplanted into the peritoneal cavity of seven, ten, and 14 days post hatching common carp fry. The results showed that the survival of seven day-old transplanted fry (31.25%) was lower than that of ten day-old (37.75%) and 14 day-old transplanted fry (56.25%). Percentage of fish colonized testicular cells donor at 21 days post-transplantation on seven days old and ten days old fry were similar (80%), while on 14 day-old fry was 60%. Based on the cumulative transplantation success rate (survival and colonization rates), transplantation on 14 days old fry (33.75%) showed higher result compared to transplantation on seven days old fry (25.00%) and ten day-old fry (30.00%). It can be concluded that transplantation of neon tetra testicular cells to common carp fry have been successfully carried out, and the optimum age of common carp fry to transplantation was 14 days after hatching. Keywords: transplantation, colonization, testicular cells, common carp, neon tetra  ABSTRAK Ikan neon tetra Paracheirodon innesi merupakan ikan hias yang memiliki nilai ekspor yang tinggi. Namun demikian, tingkat produksinya masih relatif rendah karena fekunditas ikan neon tetra yang sedikit (sekitar 180 telur/induk). Teknologi transplantasi sel testikular ikan neon tetra (ikan donor) ke ikan mas yang memiliki fekunditas telur yang banyak dan diharapkan mampu mengatasi ketersediaan benih ikan neon tetra. Penelitian ini bertujuan untuk menentukan umur optimum benih ikan mas (calon ikan semang) yang mampu menerima sel donor dengan baik dan memiliki keberhasilan kolonisasi yang tinggi. Testis ikan neon tetra didisosiasi menggunakan larutan tripsin 0,5%. Sel testikular diwarnai dengan PKH-26, kemudian ditransplantasikan ke rongga peritoneal benih ikan mas umur tujuh, sepuluh, dan 14 hari setelah menetas. Hasil penelitian menunjukkan bahwa tingkat kelangsungan hidup ikan mas perlakuan transplantasi umur tujuh hari (31,25%) lebih rendah dibandingkan dengan perlakuan transplantasi umur sepuluh hari (37,50%) dan 14 hari (56,25%). Persentase ikan terkolonisasi sel donor pada hari ke-21 pascatransplantasi pada benih umur tujuh dan sepuluh hari adalah sama (80%), sedangkan transplantasi benih umur 14 hari sebesar 60%. Berdasarkan keberhasilan transplantasi secara kumulatif (tingkat kelangsungan hidup dan kolonisasi), transplantasi pada benih umur 14 hari (33,75%) menunjukkan hasil lebih tinggi dibandingkan dengan perlakuan transplantasi pada benih umur tujuh hari (25,00%) dan benih umur sepuluh hari (30,00%). Transplantasi sel testikular ikan neon tetra pada benih ikan mas telah berhasil dilakukan, dan umur optimum benih ikan mas adalah 14 hari setelah menetas. Kata kunci: transplantasi, kolonisasi, sel testikular, ikan mas, ikan neon tetra