Agung Budiyanto
Bagian Reproduksi dan Kebidanan Fakultas Kedokteran Hewan Universitas Gadjah Mada Yogyakarta

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MOLECULAR EXPRESSION OF WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER 4 GENE USING Escherichia coli BL21 Sitasiwi, Agung Janika; Artama, Wayan Tunas; Budiyanto, Agung; Dharmana, Edy
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

This research was conducted to find out the Wnt4 recombinant proteins which expressed by Escherichia coli (E. coli) BL21 carrying the recombinant DNA wnt4 (E. coli transformation). Research materials were E. coli BL21 transformation and E. coli BL21 non-transformation (negative control). The expression of recombinant protein was conducted by culturing E. coli for 24 hours in Luria-Bertani (LB) media with isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. Recombinant protein was isolated by sonication of pellet bacteria. Protein analysis performed by 15% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that recombinant protein with a molecular weight of 33 kDa has been expressed by E. coli BL21 transformation successfully. 
Review of The Artificial Insemination Result Based on Anestrus Post Insemination in Bali Cattle Herds at The Regency of Sikka, East Nusa Tenggara Topianong, Tarsisius Considus; Budiyanto, Agung; Setyawan, Erif Maha Nugraha
Jurnal Sain Veteriner Vol 32, No 1 (2014)
Publisher : Fakultas Kedokteran Hewan

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Anestrus is one of indirect clinical signs of cattle pregnancy. Based on the present of sufficient progesterone on the blood,  the cattle with pregnancy condition will not show the signs of estrus.  The success of artificial insemination (AI) is the occurrence of fertilization followed by pregnancy and parturition. This involves a complex relationship between semen quality and oocytes, estrus detection, AI punctuality, inseminator and farmers ability. Artificial insemination should be performed at the relative optimum time to ovulation. Estrus detection is an important factor to determine the time of AI. Estrus Detection of Bali cattles of the farmer is often difficult observed or not observed, especially in semi-intensive system maintenance. From this review it can be seen that there is the different proportion of inseminators on the incidence of estrus after AI. However, determination of  the role of inseminator on the success of AI and  then the other factors that affect the success of the AI should be under ideal conditions. Straw from the different bulls breed gave the same of AI result. The estimate of pregnancy rate of 80 cows after AI is 55 %.  Estrus detection on 21st days after AI can be used as a detection method for early diagnosis of pregnancy, especially for the  farmers who did not have any ability or authority to perform  medical acts of reproduction which is only owned by a veterinarian. Observation, recording and evaluation of the implementation of the AI should be made until the end of pregnancy  followed by the process of parturition.
Effect of Ovaries T ransport Time to Quality Morphology of Cattle Oocytes In V itro Anggoro, Dito; Budiyanto, Agung
Jurnal Sain Veteriner Vol 33, No 1 (2015)
Publisher : Fakultas Kedokteran Hewan

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Abstract

One of the most important factors in the production of in vitro embryos is oocyte quality . The excellent of oocyte quality will also produce a level of excellent cleavage and lastocyst. Transportation time from the abattoir (slaughterhouse) to the laboratory is one of the actors reported to influence the quality of the oocyte. The transportation time is right to produce oocytes with the best morphological quality has never been done for ovarian cattle in indonesia. This research was conducted to determine the effect of long time transportation to the quality of ovarian morphology of cattle oocytes collected in vitro. This research consisted of three stages: the collections of both ovarians and oocyte and evaluation of the oocytes quality as well. Ovarian collection done in slaughterhouse and the collected ovarians were then taken to the laboratory for oocyte collection process performed by the method of aspiration. Ovaries are grouped based on the time of transportation 2, 3, 4, and 5 hours, respectively . Obtained oocytes were evaluated to determine their qualities by classifying into quality A, B, C, and D. In this study , it was shown that there was a statistically significant differences between the longer transport time to the quality of the morphology of the bovine ovarian oocytes (P <0.05). This study suggests that a long period of transportation is related to the ovarian morphology of oocyte quality . The ovaries that were being subjected to transportation for 2 hours produces the percentage of oocytes with morphological quality A and B which were better compared to that of ovaries being subjected to transportation for more than 2 hours.
Gangguan Reproduksi Sapi Bali pada Pola Pemeliharaan Semi Intensif di Daerah Sistem Integrasi Sapi - Kelapa Sawit Budiyanto, Agung; Tophianong, Tarsisius Considus; Triguntoro, .; Dewi, Henny Kusuma
P-ISSN 2337-3202
Publisher : Bogor Agricultural University

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Abstract

Pemeliharaan sapi Bali di Kotamadya Bengkulu dengan sistem Sistem Integrasi Sapi - Kelapa Sawit (SISKA) sudah berjalan beberapa tahun. Salah satu parameter keberhasilan program ini adalah performa reproduksi sapi Bali. Performa reproduksi sapi Bali menggambarkan kualitas dari sistem manajemen pemeliharaan yang telah dilakukan. Kajian performa reproduksi sapi Bali tersebut sudah dilakukan dengan pemeriksaan reproduksi secara per rektal dan analisa data recording peternak dan petugas. Tujuan utama dari program manajemen reproduksi adalah mendapatkan produksi yang optimal dan keuntungan yang maksimal. Efisiensi reproduksi menentukan produktivitas, profitabilitas dan keberlanjutan dari setiap usaha peternakan. Adanya gangguan reproduksi menyebabkan inefisiensi reproduksi. Kondisi ini akan menyebabkan kerugian ekonomi. Tujuan penelitian ini adalah untuk mengetahui kondisi gangguan reproduksi dan respon kesembuhannya. Sebanyak 333 ekor sapi Bali betina dengan umur minimal 2 tahun dilakukan pemeriksaan reproduksi. Metode penelitian dilakukan melalui beberapa tahap yaitu anamnesa, pemeriksaan klinis dan pemeriksaan reproduksi secara per rektal. Penanganan gangguan reproduksi dinyatakan sembuh apabila timbulnya respon klinis berupa estrus. Data yang diperoleh kemudian dicatat dan dianalisa secara deskriptif. Berdasarkan hasil pemeriksaan diketahui bahwa 193 (57,95 %) sapi betina mengalami gangguan reproduksi yang meliputi delayed pubertas, hipofungsi ovarium, metritis, endometritis dan anestrus postpartum. Sedangkan sebanyak 80 (41,45 %) sapi sudah menunjukan gejala estrus. Adanya interaksi yang kompleks antara faktor lingkungan atau manajemen (nutrisi), respon individual, jenis gangguan reproduksi dan derajat keparahan gangguan reproduksi akan menimbulkan respon kesembuhan yang bervariasi dari setiap penanganan gangguan reproduksi.Kata kunci: gangguan reproduksi, sapi Bali, estrus, Bengkulu. (Bali Cattle Reproductive Disorders with Semi Intensive Management in The Area of Cattle - Oil Palm Integration System)The maintenance of Bali cattles in Bengkulu regency with cattle - oil palm integration system (SISKA) has been running several years. The one parameters of the success this program is the reproductive performance of Bali cattle. Bali cattle reproductive performance describe the quality of the maintenance management system that has been done. Bali cattle reproductive performance study has been conducted with reproductive rectal examination and analysis of the data recording breeders and farmer. The main purpose of the reproductive management program was getting the optimal production and maximum benefit. Reproductive efficiency determines the productivity, profitability and sustainability of each farm. The inefficiency reproductive was caused by existence of reproductive disorders. These conditions cause economic losses. The purpose of this study was to determine the condition of reproductive disorders and recovery response. A total of 333 cows Bali females with at least 2 years of age has been reproductive examination. The research methods were done through classification for several stages, anamnesis, clinical examination and reproductive examination by rectally palpation. Treatment of reproductive disorders declared cured if the onset of clinical response in the form of estrus. The data obtained then were recorded and analyzed descriptively. Based on the results of the examination reported that 193 (57.95%) of female Bali cattles experiencing reproductive disorders which include delayed puberty, ovarian hypofunction, metritis, endometritis and postpartum anestrus. While as many as 80 (41.45%) of female Bali cattles were showing signs of estrus. The existence of complex interactions between environmental factors or management (nutrition), individual responses, the type and severity of reproductive disorders were affected of varies healing response from each treatment of reproductive disorders.Keywords: reproductive disorders, bali cattle, oestrus, Bengkulu.
Korelasi Berat Badan dan Umur Sapi terhadap Berat, Volume Cairan dan Konsentrasi Prostaglandin F2α pada Vesikula Seminalis Dalimunthe, Naela Wanda Yusria; Budiyanto, Agung; Setyowati, Erna Prawita; Wijayanti, Agustina Dwi
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Abstract

Seminal vesicles were collected from 60 heads of Bulls which butchered in slaughter house (RPH) Yogyakarta. The aims of this study are knowing the relationship between body weight, age, fluids volume and concentration of prostaglandin F2 α (PGF2α) in seminal vesicle of beef cattle. Those seminal vesicles were gathered from bulls which recorded its body weight and age then measured its seminal vesicles for weight, fluids volume and PGF2α levels. The PGF2α level was measured by Enzyme-Linked Immunosorbent Assay. Statistical analysis was performed using one way – analysis of varian, regression and correlation with P<0.05. Body weight of bulls showed positive correlation with the weight of seminal vesicle and its fluids volume. However, PGF2α levels were not correlated with the body weight of cattle. Weight of seminal vesicles also exhibited positive correlation with volume of vesicle fluids but no correlation with PGF2α levels. Based on the age of bulls, there were no correlation withthe weight of seminal vesicles, seminal fluids volume and PGF2α levels. Those result indicated that the weight and fluids volume were affected by the body weight of bulls altough the PGF2α levels have a standard of developmentwhich seems affected by other factors such as concentration of androgen hormone.
Review of The Artificial Insemination Result Based on Anestrus Post Insemination in Bali Cattle Herds at The Regency of Sikka, East Nusa Tenggara Topianong, Tarsisius Considus; Budiyanto, Agung; Setyawan, Erif Maha Nugraha
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Abstract

Anestrus is one of indirect clinical signs of cattle pregnancy. Based on the present of sufficient progesterone on the blood,  the cattle with pregnancy condition will not show the signs of estrus.  The success of artificial insemination (AI) is the occurrence of fertilization followed by pregnancy and parturition. This involves a complex relationship between semen quality and oocytes, estrus detection, AI punctuality, inseminator and farmers ability. Artificial insemination should be performed at the relative optimum time to ovulation. Estrus detection is an important factor to determine the time of AI. Estrus Detection of Bali cattles of the farmer is often difficult observed or not observed, especially in semi-intensive system maintenance. From this review it can be seen that there is the different proportion of inseminators on the incidence of estrus after AI. However, determination of  the role of inseminator on the success of AI and  then the other factors that affect the success of the AI should be under ideal conditions. Straw from the different bulls breed gave the same of AI result. The estimate of pregnancy rate of 80 cows after AI is 55 %.  Estrus detection on 21st days after AI can be used as a detection method for early diagnosis of pregnancy, especially for the  farmers who did not have any ability or authority to perform  medical acts of reproduction which is only owned by a veterinarian. Observation, recording and evaluation of the implementation of the AI should be made until the end of pregnancy  followed by the process of parturition.
The Effects of Reciprocal Teaching Learning Strategy and Self efficacy on Learning Outcomes of Early Childhood (AUD) Mathematical Logic Erwanto, Erwanto; Maryatmi, Anastasia Sri; Budiyanto, Agung
Al-Jabar: Jurnal Pendidikan Matematika Vol 9, No 1 (2018): Al-Jabar: Jurnal Pendidikan Matematika
Publisher : Raden Intan State Islamic University of Lampung, INDONESIA

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Abstract

The purpose of this research is to learn about the influence of Reciprocal Teaching and Self Efficacy on learning outcomes of AUD mathematical logic. This research is experimental research designed using treatment by level 2 × 2. The research method used is a variance analysis of two different cell paths. The results of this research are: (1) learning outcomes of students using Reciprocal Teaching strategy is higher than Ekspository strategy, (2) there is an interaction influence between learning strategy and self efficacy on learning outcomes of AUD mathematical logic, (3) there is an influence of self efficacy on learning outcomes of AUD mathematical logic. 
TINGKAT MATURASI IN VITRO PADA OOSIT SAPI SILANGAN SIMMENTAL PERANAKAN ONGOLE DAN LIMOUSIN PERANAKAN ONGOLE Parera, Hermilinda; Budiyanto, Agung
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): J. Ked. Hewan
Publisher : Syiah Kuala University

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Penelitian ini bertujuan mengetahui tingkat maturasi oosit sapi Simmental Peranakan Ongole (SimPO) dan Limousin Peranakan Ongole (LimPO) secara in vitro. Oosit dari Ovarium sapi yang berasal dari rumah potong hewan (RPH), dikelompokkan berdasarkan jenis sapi PO (kontrol), SimPO dan LimPO. Oosit diaspirasi dari ovarium dengan syringe 5 ml dan jarum 18 G. Oosit dengan kualitas A dan B yang digunakan dalam penelitian ini. Oosit dikultur dalam media maturasi TCM 199 100 µl drop dan dilapisi minyak mineral, diinkubasi pada suhu 38,5° C, CO2 5% dan kelembaban 95% selama 24 jam. Tingkat maturasi in vitro ditentukan dengan pewarnaan aceto orcein 1% untuk melihat tahapan maturasi dengan adanya perubahan konfigurasi kromosom dan membran inti berupa germinal vesicle, germinal vesicle breakdown, metafase I, anafase/telofase I, dan metafase II. Hasil penelitian menunjukkan tingkat maturasi in vitro oosit yang mencapai metafase II dari oosit sapi PO lebih tinggi secara signifikan dibandingkan sapi SimPO dan LimPO.
Optimalisasi Pembekuan Sperma Limbah Kauda Epididimis Kambing Lokal dengan Metode Bertahap dan Stabilisasi Dalimunthe, Naela Wanda Yusria; Ridlo, M. Rosyid; Budiyanto, Agung
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Abstract

Buck slaugthering produce waste such as testicles including epididymis which contain fertile sperm. Utilization of cauda epididymis as the sources of sperm for producing goat frozen sperm was not reported yet. The aims of this study were improving the frozen-thawed sperm using stabilization and multistep methods which recovered from the waste of buck slaughtering as the source of sperma. Cauda epididymis spermatozoa which was washed then diluted using extender 1 (Tris-citrate-antibiotics) and extender 2 (extender 1- glycerol-egg yolk). The extender 2 addition was performed by single or multistep methods then freezed. Modification in the pre freezing proces were performed by comparing the conventional equilibration and stabilization methods. The sperm suspension was incubated in 4°C for 2 hours after filling-sealing into straws on the equilibration group whether the stabilization group was cooled in tube 15 mL. All cooled straws from both groups were placed 4 cm horizontally on liquid nitrogen surface for 10 minutes and then plunged into liquid nitrogen for storage. The evaluation of motility parameters such as pattern of the movement and motility percentation were done followed the standard methodology. The student t-test, correlation and one-way ANOVA were used for data analysis with P<0.05. The results showed that multistep dilution method could increase the motility (25.0 ± 1.8 %) compared with single step (18.3 ± 1.7 %). Pre freezing method with stabilization also resulted higher motility (24.2 ± 2.0 %) than equilibration method (17.5 ± 2.8 %). The pattern of the movement were not different between all methods and its combination. The multistep dilution method and stabilization cooling method as well as its combination seems could increase the quality of frozen-thawed cauda epididymis spermatpzoa of local buck.
UJI ORGANOLEPTIK NUGGET DAGING DADA ITIK AFKIR DENGAN JENISDAN DOSIS TEPUNG YANG BERBEDA Rahmawati, Nurina; Budiyanto, Agung
Jurnal Ilmiah Fillia Cendekia Vol 2 No 1 (2017): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

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Abstract

One of the resilience and diversification program of food were supplying and using local meat. Duck’s meat was soource of highly animal protein, but less of idea to process that meats. Nuggets was the produst processed meat that well liked many people. So expcted an be increase the economic of society. This research conducted at the campus of the Islamic University Kadiri on 29 april 2015 and was herped by 30 panelist. The purpose of this study to determine the species and levels or the different flour in nuggets ducks so that consumers prefer. The data obtained were analized using a completely randomized design analysis and if there is a noticeable difference then proceed to test LSD. The result showed that the species and levels or the different flour in nuggets ducks gave highly significant (P&lt;0,01) for organoleptic tests include color, aroma, taste and texture. The conclusion of this research was the panelis liked the use of &nbsp;20% level of flour in nuggets ducks