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The effects of duration of mesenteric artery ligation to the ratio of TNF-α/IL-10 in a rat model of acute mesenteric ischemia (AMI) Handaya, Adeodatus Yuda; Budipramana, Vicky S.
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 49, No 4 (2017)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/JMedSci004904201702

Abstract

The mortality rate of acute mesenteric ischemia (AMI) is high due to the delay in diagnosis.Determination of potent biomarker for early AMI is the key in reducing the mortality. As aproinflammatory cytokine, the level of TNF-α might be affected during the ischemia andreperfusion, with the prediction duration of 60-120 min. High TNF-α level may stimulatethe upregulation of IL-10 as an inhibitor of TNF-α. This provides a new opportunity forearly diagnosis of AMI by measuring the ratio between those two cytokines. The purposeof this study was to investigate the effect of duration of the mesenteric artery to theratio of TNF-α/IL-10 in a AMI rat model. This was an experimental study using Wistarrat. We performed mesenteric artery in 28 male rats to produce an AMI model, withligation duration of 0, 30, 60, 90, 120, 150, and 180 minutes. At the end of ligation,blood samples were taken for measurement of TNF-α and IL-10 level using ELISA. For themicroscopic examination of tissue necrosis, intestinal organ samples were taken and madeinto paraffin blocks and stained using Haematoxylin-Eosin. TNF-α increased in minute 120compared to other treatment groups (p<0.05). IL-10 increased in minute 180 comparedto control group (p<0.05). Microscopic examination showed that the duration of ligationaffects the structure and morphology of intestinal mucosa characterized by discolorationof organs along with increasing the ligation duration. Ligation of the superior mesentericartery was found to be significantly increased the TNF-α level and to be compensated byincreasing IL-10. It is assumed that when the IL-10 level, that has protective effect as aninhibitor, higher than TNF-α level as a proinflammatory cytokine on duration 150 minutes,it means no more inflammatory or cells is dead. Therefore TNF-α/IL-10 ratio can be usedas a biomarker candidate of prognosic factor management of AMI.
Correlation between alkaline phosphatase, g-glutamyl transpeptidase, and bilirubin with interleukin-1b level in dogs with obstructive jaundice Setyawan, Nurcahya; Budipramana, Vicky S.
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 4 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.555 KB)

Abstract

Surgical management in obstructive jaundice still contributes to significant morbidity and mortality. One of complications following surgery in obstructive jaundice is sepsis. This complication is caused by the toxic effects of bilirubin and bile salts, endotoxins, bacterial translocation, modulation of the immune-inflammatory cascade, decreased cellular immunity and/or nutritional status. Many studies have shown the elevated inflammatory response indicator, interleukin-1 (IL-1b), in patients with obstructive jaundice. However, only a few report described the association between the indicators of obstructive jaundice (alkaline phosphatase [ALP], g-glutamyl transpeptidase [GGT], and bilirubin)and the indicator of inflammatory response (interleukin-1b [IL-1b]). This study aimed to investigate the association between the indicator of obstructive jaundice (ALP, GGT, and bilirubin) and the level of interleukin-1b (IL-1b) in dogs as the animal model. We performed ligation on distal common bile ducts (CBD) to produce a model of obstructive jaundice. Every three days within a month, the blood samples from ten dogs were extracted to determine the ALP, GGT, direct and total bilirubin, and IL-1b levels. We found a significant correlation between the ALP and GGT with IL-1b level with p-value of 0.036 (r=0.626) and 0.003 (r=0.826). However, there was no association between the increased directbilirubin with the IL-1b level (p=0.068; r=0.537). Moreover, the increased level of ALP and GGT had a strong correlation with the increased level of direct bilirubin with p-value of 0.004 (r=0.810) and p=0.011 (r=0.746). In conclusion, the increased level of GGT was the strongest indicator for inflammatory response in dogs with obstructive jaundice. Furthermore, the increased levels of GGT and ALP might imply the development of obstructive jaundice in dogs.
THE ROLE OF CARCINOEMBRYONIC ANTIGEN IN ASSESSING THE SUCCESS OF SURGICAL TREATMENT IN COLORECTAL CANCER BASED ON STAGING Widyasari, Anindya; Tambunanan, Betty Agustina; Budipramana, Vicky S.
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1333

Abstract

Colorectal cancer is the third most common cancer in Indonesia. Determination of staging is needed to determine the treatment of colorectal cancer which has four stages. Carcinoembryonic Antigen (CEA) is a serological marker for monitoring colorectal cancer status, prognostic determination, monitoring of treatment success, detecting early recurrence and spreading. The purpose of this study was to determine the evaluation of successful surgical therapy in colorectal cancer by CEA examination at each stage. Descriptive observational studies were conducted using secondary data of pre and post-surgical colorectal cancer patients examined for CEA and treated at the Dr. Soetomo Hospital from January 2015 to December 2016. The samples obtained from this study were 48 patients, with the most of them at the age of 41-60 years as much as 70.83%. Females were more than males (66.67% vs. 37.33%). The most staging stage, stage 4 was as much as 43,75% followed by stage 3 as much 41.67%, the rest were stage 1 as much as 10.42%, and stage 2 as much as 4,17%. The highest decrease in CEA levels was found in stage 4 by 85%, followed by stage 2 of 53.5%, stage 1 of 43.4% and stage 3 of 33.1% but statistically only the decrease in stage 3 was significant. In stage 1, there was a difference in pre-operative CEA with a mean of 3.09 ng/mL (0.17-5.83 ng/mL) vs. post-operative with a mean of 1.75 ng/mL (0.84-3.14 ng/mL), stage 2 levels of pre-operative CEA with a mean of 3.82 ng/mL (0.15-7.48 ng/mL) vs. post-operative with a mean 1.77 ng/mL (1.46-2.08 ng/mL), stage 3 levels of pre-surgical CEA with a median of 13.85 ng/mL (1.09-71.21 ng/mL) vs. post-operative with a median 9.26 ng/mL (<0.5-68.23 ng/mL), stage 4 pre-surgical CEA levels with a median 183.77 ng/mL (0.54-2861 ng/mL) vs. post-operative with a median 27.28 ng/mL (0.51-155.10 ng/mL). There was a decrease in CEA levels from the total number of patients by 67%, whose CEA levels remained at 12% and as much as 21% of their CEA levels increased. Successful evaluation of surgical therapy in the colon and rectal cancer by CEA examination was still varied at each stage where CEA levels decreased significantly in stage 3.