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ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI MAKANAN FERMENTASI BEKASAM IKAN MUJAIR (Oreochromis mossambicus Peters)

Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bekasam is one of the traditional fermented food made from freshwater fish with the addition of salt and carbohydrate sources. Mozambique fish is used as a raw material, because it has high protein content and can be easily obtained in the market at affordable prices. Mozambique fish is used to make bekasam with the addition of salt as much as 18% of the weight of the fish and brooded for 48 hours, then added with toasted rice as much as 15% of the weight of the fish and fermented for 7 days. This study aimed to isolate and characterize lactic acid bacteria and analyze the nutritional value of mozambique fish bekasam. The methods used were  isolation, characterization and proximate analysis of the mozambique fish bekasam. Isolation of lactic acid bacteria was carried out on mozambique fish as a control, mozambique fish before and after the addition of toasted rice. Isolation obtained 6 isolates of Gram positive bacteria, 5 isolates of cocci shaped bacteria and 1 rod shaped bacteria. Based on morphological and physiological tests, six isolates were Gram positive, non motile, catalase negative, positive to produce acid and had proteolytic activity. Bekasam mozambique fish contained as much as 0.64% lactic acid and pH is 5.39. The results of the proximate analysis bekasam mozambique fish showed that the fermentation process increased the nutritional value bekasam quality mozambique fish with 5.5270% water content, 33.0628% ash content, 0.0788% crude fiber content, 45.0546% crude protein content, 7.9419% crude fat content and 13.9407% carbohydrate content. Keywords : bekasam , lactic acid bacteria , isolation, characterization, proximate analysis

ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI RUSIP IKAN TERI (Stolephorus sp.)

Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rusip is a traditional food from Bangka-Belitung made from fresh anchovy with salt and palm sugar that is brooded for seven days. Rusip fermentation process is caused by microbial activity mainly from lactic acid bacteria. The purpose of this study was to isolate lactic acid bacteria from rusip, to characterize isolates obtained based on common characteristic of lactic acid bacteria, and to carry out proximate analysis of rusip anchovy. Isolation from rusip fermented food obtained eight bacterial isolates, five isolates were circular and three isolates were rod-shaped. Characterization and identification of lactic acid bacterial were performed using tests that led to the common characteristic of lactic acid bacteria, i.e the gram staining, motility test, acid formation test, catalase test, and proteolytic test. The result showed that eight bacterial isolates were gram-positive, non-motile, catalase-negative, positive to produce acid, and had proteolytic activity. Rusip anchovy had 0,64% of total lactic acid and the pH 5,40. Nutrient content of rusip fermentation had 10,3644% moisture content; 50,0702% ash content; 0,9524% crude fat content; 0,3764% crude fiber content; 34,8603% crude protein content; and 14,1171% carbohydrate content. Keywords: rusip, isolation, characterization, lactic acid bacteria, proximate analysis

TRANSFORMASI DAN KLONING PLASMID PJ804:77539 PADA E.coli TOP’10

Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Kloning dan transformasi vektor PJ804:77539 dilakukan dengan tujuan perbanyakan vektor pRHA pada sel bakteri E.coli TOP’10. Ekpresi vektorpRHA diharapkan dapat terjadi pada periplasma E.coli dan memberikan ekspresi berupa kemampuan resistensi terhadap Ampicillin. Ekspresi pada periplasma bertujuan untuk meminimalisir kerugian yang timbul pada sistem ekspresi di sitoplasma di antaranya tingkat ekspresi yang rendah, protein terpotong atau resiko kontaminasi. Sekresi protein rekombinan pada periplasma dapat meningkatkan aktivitas biologis serta tingkat kestabilan produk menjadi lebih tinggi. Proses isolasi protein yang diekspresikan pada periplasma  dapat dilakukan dengan perlakuan stress osmotik ringan sehingga menurunkan resiko kontaminasi protein sitoplasma. Ekspresi protein pada periplasma diarahkan oleh peptida sinyal pelB. Peptida sinyal bekerja menarik produk protein ke periplasma dengan cara berfusi dengan ujung N-terminal pada peptida yang terekspresi. Penanda selektif (selectable marker) yang terdapat pada PJ804::77539 merupakan Ampr, suatu penanda yang memampukan bakteri untuk resisten pada keberadaan antibiotik Ampicillin. Transformasi dilakukan sesuai dengan metode heat – shock dan diseleksi pada medium LB agar dan LB cair yang mengandung antibiotik Ampicillin dengan konsentrasi 100 mg/mL. Diperoleh koloni tumbuh pada medium yang mengandung Ampicillin dan dilakukan isolasi plasmid. Visualisasi hasil elektroforesis memperlihatkan adanya pita plasmid yang diisolasi dari E.coli TOP’10 pada gel elektroforesis.Kata kunci : Ampicillin, E.coli, pelB, periplasm dan pRHA

BIOPROSPEKSI BAKTERI YANG BERASOSIASI DENGAN ALGA HIJAU HALIMEDA MACROLOBA, CAULERPA RACEMOSA, DAN ULVA SP SEBAGAI PENGHASIL SENYAWA ANTIBAKTERI

Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacteria can grow in various environments, including those associated with marine organisms such as algae, sponge, sea grass and soft corals. For these organisms, bacteria help building their defense by producing secondary metabolites such as bioactive compound.  This research aims to study the potency of the bacteria which interact with green algae Halimeda macroloba, Caulerpa racemosa, and Ulva sp which are able to produce antibacterial compound. Algae, which belong to Chlorophyta, are common to be found in marine water. This research used bacterial isolation, morphologic bacterial isolate characterization, antibacterial test, molecular identification of the antibacterial compound producer isolate, and biochemical activity test. From the isolation, the researcher was able to collect five bacterial isolate; one from H. macroloba, three from C. racemosa, and one isolate from Ulva sp. Of the five isolates, one isolate from C. racemosa can prevent the E.coli, and one isolate from H. macroloba can prevent E.coli and P.aeruginosa. Isolate derived from Halimeda macroloba have the biggest prevention zone ability, which is 18.1 mm, towards P.aeruginosa. Keywords: Antibacterial, Bacterial Association, Halimeda macroloba, Caulerpa racemosa,      Ulva sp

EKSPLORASI DAN KARAKTERISASI BAKTERI POTENSIAL PENGHASIL SENYAWA ANTIFOULING YANG BERASOSIASI DENGAN ALGA COKLAT (PHAEOPHYTA) DI PERAIRAN KEPULAUAN KARIMUNJAWA JEPARA

Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biofouling is a settlement process from marine organisms initiated by biofilm (microbial layers). Corrosion on the vessels, pipelines under the sea, oil plants, etc are big problems in the industrial today cause adversely affects. Antifouling paints have been developed to prevent settlement of organisms, however antifouling paints contain heavy metals and biocides which have toxic effects on marine ecosystems. Antifouling compound which environmental friendly is a solution for one of the paint component. The aim of this research was to isolate bacteria associated with brown algae which had potential to produce antifouling compound from Karimunjawa islands, Jepara.Isolate KS1-1 showed antifouling activity against Pseudomonas aeruginosa as biofilm-forming bacteria.This isolate had been characterized in morphology, moleculer and biochemichal identification.Moleculer analyses of 16S rRNA sequence, KS1-1 showed similarity 94% from 287 bp with Vibrio sp. W-137-16S ribosomal RNA gene, partial sequence. Keywords : Antifouling, Phaeophyta,Biofouling, 16S rRNA, Vibrio

Bioprospeksi Bakteri Yang Berasosiasi Dengan LamunEnhalus acoroidesdan Syringodium isoetifoliumSebagai Penghasil Senyawa Antibakteri

Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacteria can grow in variety of environments including those associated with marine organisms such as seagrasses, sponge, algae and soft corals. Bacteria association has the ability to produce bioactive compounds that can be used as a defense against pathogens and their hosts by producing secondary metabolites such as antibacteria compounds. This study aimed to asses the potential of bacteria associated with seagrasses Enhalus acoroides and Syringodium isoetifolium. This study was conducted with bacterial isolation from seagrass E. acoroides and S. isoetifolium, characterization of bacterial isolatesin morphology, antibacterial test, and biochemcal activity test. The result obtained four bacterial isolates, two isolates of Syringodium isoetifolium and two isolates of Enhalus acoroides. Two isolates from Syringodium isoetifolium had the largest ability inhibitory zone of inhibtion against the bacteria P. Aeruginosa (5.7mm) and against the bacteria E. coli (6.65mm).Key word : Antibacterial Compound, Seagrass-Associated Bacteria, Vibrio, Enhalus acoroides,      Syringodium isoetifolium

IDENTIFIKASI ISOLAT MONASCUS SP. HASIL ISOLASI ANGKAK BERDASARKAN GEN INTERNAL TRANSCRIBED SPACER (ITS) DAN PENGUKURAN KANDUNGAN PIGMEN

Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The Identification of Monascus based on their morphological characteristic, this methods is very problematic due to several  specimens share similarity on morphology and colours. Nowadays, the identification of molecular based organisms has been done to complement morphological identification, for example mold identification using the Internal Transcribed Spacer (ITS) gene. The objectives of study were to identify of Monascus isolates the result of angkak isolation using Internal Transcribed Spacer (ITS) gene, phylogenetic analyzing,  determination cell growth and determination of production pigment content. Sequences of  ITS Monascus were amplified using PCR and the molecular phylogenetic  analyses was using Neighbor Joining (NJ) method. Based on homology search by Basic Local Alignment Search Tool (BLAST) program and phylogenetic tree analyses, mold of isolates were identified as the Monascus purpureus (100%). The amplified DNA fragments were about 553 bp. Determination of Monascus mold cell growth were carried out by the method of dry weight cell. Determination of pigment by using spectrophotometer at 390 nm wavelength for yellow pigment and 500 nm for red pigment. The result of determination of Monascus mold pigment content show that have increased during fermentation time until day 13. The amount of yellow extracellular pigment production is 37.358 U / g; While the red color pigment of 2.6545 U / g. The amount of red intracellular pigment production is 7.4175 U / g, while the yellow pigment is 30.176 U / g.Keywords: Monascus, angkak, molecular phylogenetic, pigment

Deteksi Jenis Padi Indica dan Japonica Padi Gogo Rancah Beras Merah Varietas Slegreng dan Mandel Berbasis Fragmen ORF100 dan ORF29

JURNAL SAINS DAN MATEMATIKA Volume 21 Issue 4 Year 2013
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Deteksi fragmen Open Reading Frame (ORF)100 dan ORF29 telah dilakukan pada dua jenis padi gogo rancah beras merah yaitu varietas Slegreng dan Mandel. Metode penelitian dilakukan dengan cara isolasi DNA kloroplas padi diikuti dengan amplifikasi PCR menggunakan optimasi suhu annealing pada tiga suhu yang berbeda, yaitu 53°C, 55°C, dan 56°C. Selanjutnya hasil amplifikasi divisualisasikan menggunakan elektroforesis pada gel agarose 1%. Suhu annealing 53°C merupakan suhu yang optimal untuk memperlihatkan fragmen ORF100 dan ORF29. Hasil penelitian menunjukkan bahwa fragmen ORF100 dan ORF29 dapat teramplifikasi pada padi Slegreng dan mandel, sehingga kedua jenis padi tersebut menunjukkan kecenderungan subspesies japonica. Perbedaan ketebalan dan ukuran pita tidak mempengaruhi keberadaan fragmen ORF100 dan ORF29 sebagai penanda untuk mengidentifikasi tipe indica atau japonica. Pemanfaatan marka molekuler fragmen ORF100 dan ORF29 dalam mendeteksi jenis padi diharapkan dapat melengkapi hasil karakterisasi dan pengelompokkan varietas padi berdasar karakter morfologi dan fisiologi.   Keywords: chloroplast DNA, ORF100, ORF29, indica-japonica differentiation

Deteksi Jenis Padi Indica dan Japonica Padi Gogo Rancah Beras Merah Varietas Slegreng dan Mandel Berbasis Fragmen ORF100 dan ORF29

SAINS DAN MATEMATIKA Volume 21 Nomor 4 Tahun 2013
Publisher : SAINS DAN MATEMATIKA

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Abstract

Deteksi fragmen Open Reading Frame (ORF)100 dan ORF29 telah dilakukan pada dua jenis padi gogo rancah beras merah yaitu varietas Slegreng dan Mandel. Metode penelitian dilakukan dengan cara isolasi DNA kloroplas padi diikuti dengan amplifikasi PCR menggunakan optimasi suhu annealing pada tiga suhu yang berbeda, yaitu 53°C, 55°C, dan 56°C. Selanjutnya hasil amplifikasi divisualisasikan menggunakan elektroforesis pada gel agarose 1%. Suhu annealing 53°C merupakan suhu yang optimal untuk memperlihatkan fragmen ORF100 dan ORF29. Hasil penelitian menunjukkan bahwa fragmen ORF100 dan ORF29 dapat teramplifikasi pada padi Slegreng dan mandel, sehingga kedua jenis padi tersebut menunjukkan kecenderungan subspesies japonica. Perbedaan ketebalan dan ukuran pita tidak mempengaruhi keberadaan fragmen ORF100 dan ORF29 sebagai penanda untuk mengidentifikasi tipe indica atau japonica. Pemanfaatan marka molekuler fragmen ORF100 dan ORF29 dalam mendeteksi jenis padi diharapkan dapat melengkapi hasil karakterisasi dan pengelompokkan varietas padi berdasar karakter morfologi dan fisiologi.   Keywords: chloroplast DNA, ORF100, ORF29, indica-japonica differentiation

Deteksi Jenis Padi Indica dan Japonica Padi Gogo Rancah Beras Merah Varietas Slegreng dan Mandel Berbasis Fragmen ORF100 dan ORF29

SAINS DAN MATEMATIKA Volume 21 Nomor 4 Tahun 2013
Publisher : SAINS DAN MATEMATIKA

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Abstract

Deteksi fragmen Open Reading Frame (ORF)100 dan ORF29 telah dilakukan pada dua jenis padi gogo rancah beras merah yaitu varietas Slegreng dan Mandel. Metode penelitian dilakukan dengan cara isolasi DNA kloroplas padi diikuti dengan amplifikasi PCR menggunakan optimasi suhu annealing pada tiga suhu yang berbeda, yaitu 53°C, 55°C, dan 56°C. Selanjutnya hasil amplifikasi divisualisasikan menggunakan elektroforesis pada gel agarose 1%. Suhu annealing 53°C merupakan suhu yang optimal untuk memperlihatkan fragmen ORF100 dan ORF29. Hasil penelitian menunjukkan bahwa fragmen ORF100 dan ORF29 dapat teramplifikasi pada padi Slegreng dan mandel, sehingga kedua jenis padi tersebut menunjukkan kecenderungan subspesies japonica. Perbedaan ketebalan dan ukuran pita tidak mempengaruhi keberadaan fragmen ORF100 dan ORF29 sebagai penanda untuk mengidentifikasi tipe indica atau japonica. Pemanfaatan marka molekuler fragmen ORF100 dan ORF29 dalam mendeteksi jenis padi diharapkan dapat melengkapi hasil karakterisasi dan pengelompokkan varietas padi berdasar karakter morfologi dan fisiologi.   Keywords: chloroplast DNA, ORF100, ORF29, indica-japonica differentiation