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Association of Sexual Maturation and Body Size of Arfak Children KAWULUR, ELDA IRMA JEANNE JOICE; SURYOBROTO, BAMBANG; BUDIARTI, SRI; HARTANA, ALEX
HAYATI Journal of Biosciences Vol 19, No 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.3.124

Abstract

Gonad maturation in pubertal girls and boys is accompanied with somatic growth spurt, changes in quantity and distribution of body fat (BF), development of secondary sex characters, and relevant physiological events. Menarche (first event of menstruation) and spermarche (first event of nocturnal sperm emission) are usually used as indicators of gonad maturation. We found that median age at menarche of Arfak girls in Manokwari, West Papua is 12.2 years, while median age at spermarche of boys is 13.6 years. A possible factor causing young age at menarche is due to adaptation to unstable environmental conditions because of high risk of mortality by malaria disease during childhood. The events of menarche and spermarche achieved one year after the peak body height (BH) velocity, and just before or at the same time with the time of maximum growth rate of body weight (BW), body mass index (BMI), and BF. The average BMI of Arfak girls was big at 21.9 kg/m2 at the time of their menarche. Bigger average BMI might be caused by prepubertal slowing down of BH growth compare to growth of BW whichis still increasing. Girls accumulate BF before puberty to be used as an energy reserve for the occurrence of menarche. At the time of development of secondary sexual characters girls use the fat reserve so it decline sharply after puberty. In boys, growth rate of BF was stopped at 11 years old, and then growing negatively presumably because boys use fat mass for the occurence of spemarche. BF growth rate reached the lowest point at the age 16 years old, and then increase linearly with age through adolescence until adulthood at age 23 years old.
Phage FR38 Treatment on Sprague Dawley Rat Inferred from Blood Parameters and Organ Systems SARTIKA, DEWI; BUDIARTI, SRI; SUDARWANTO, MIRNAWATI
HAYATI Journal of Biosciences Vol 19, No 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.3.131

Abstract

The ability of phage FR38 to lysis indigenous Salmonella P38 from feces of diarrheal patient has been studied. However, effects of phage FR38 on organ system were not revealed as yet. This study was conducted to observe the effect of phage FR38 on blood chemistry, kidney functions, and liver functions. Twelve Sprague-Dawley rats were used as a model for this study that were divided into two groups; (i) control and (ii) treated group with phage FR38. For treated phage group, each rat was administered by 5 ml/kg bw of 1.59·107 pfu/ml of phage intragastric. The blood parameters were analysed on day 16. The results revealed that body and organs weight, erythrocyte, hematocrit, hemoglobin, leukocyte, total protein, creatinine, SGOT, and SGPT of phage treatment rats were not significantly different with the control rats on day 16 (P > 0.05). Therefore, this study showed was no effect of phage FR38 on body weight, blood chemistry, kidney and liver functions of the rat (P > 0.05).
The Growth of Body Size and Somatotype of Javanese Children Age 4 to 20 Years WIDIYANI, TETRI; SURYOBROTO, BAMBANG; BUDIARTI, SRI; HARTANA, ALEX
HAYATI Journal of Biosciences Vol 18, No 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.182

Abstract

Growth of body size (body height and body weight) and somatotype in 363 girls and 299 boys aged 4 to 20 years of ethnic Javanese lived in Magelang Regency Indonesia were studied cross-sectionally. Over half of them were categorized in the well-off family, therefore underweight or underfat prevalence in our subjects was low (14.3%) but overweight and obesity prevalence was also low (14%). They were shorter and lighter than reference children from U.S., Japan and Yogyakarta but they improved when compared with those of the same ethnic of Bantul and with the different ethnic of rural India. There was a clear age-related change of their somatotype. At age 4 years, the physique of children subjects in both sexes is found to be mesomorph-endomorph. Thereafter it is transformed into ectomorphic-endomorph in girls and to mesomorph-ectomorph in boys at the age of 20 years. In girl subjects, the onset of puberty was characterized by an acceleration of endomorphy component at age 8 years. While in our boys it was characterized by an acceleration of ectomorphy since age 9 years. The different growth pattern of somatotype components showed that the use of BMI as an indicator of fatness in children should be reassessed.
Extracellular Protease Activity of Enteropathogenic Escherechia coli on Mucin Substrate BUDIARTI, SRI; MUBARIK, NISA RACHMANIA
HAYATI Journal of Biosciences Vol 14, No 1 (2007): March 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.14.1.36

Abstract

Enteropathogenic Escherichia coli (EPEC) causes gastrointestinal infections in human. EPEC invasion was initiated by attachment and aggressive colonization on intestinal surface. Attachment of EPEC alter the intestine mucosal cells. Despite this, the pathogenic mechanism of EPEC infectior has not been fully understood. This research hypothesizes that extracellular proteolytic enzymes is necessary for EPEC colonization. The enzyme is secreted into gastrointestinal milieu and presumably destroy mucus layer cover the gastrointestinal tract. The objective of this study was to assay EPEC extracellular protease enzyme by using mucin substrate. The activity of EPEC extracellular proteolytic enzyme on 1% mucin substrate was investigated. Non-pathogenic E. coli was used as a negative control. Positive and tentative controls were Yersinia enterocolitica and Salmonella. Ten EPEC strains were assayed, seven of them were able to degrade mucin, and the highest activity was produced by K1.1 strain. Both positive and tentative controls also showed the ability to digest 0.20% mucin. Key words: EPEC, protease, mucin, diarrhea
Pollen Sensitivity among Respiratory Allergic Patients Rengganis, Iris; Hartana, Alex; Guhardja, Edi; Djauzi, Samsuridjal; Budiarti, Sri
Journal of the Indonesian Medical Association Vol. 58 No. 9 September 2008
Publisher : Journal of the Indonesian Medical Association

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Abstract

Allergy is a type I hypersensitivity reaction, which occurs when the body produces an excess of IgE antibody as a response to allergens. The development and severity of allergic diseases depend on a complex interaction between genetic and environmental factors. Pollen are one of the main environmental allergens. The allergenic pollens of the atmosphere varies according to climate, geography and vegetation. This research is conducted to study the pollen sensitivity reaction commonly occured among respiratory allergic (bronchial asthma and allergic rhinitis) patients in Jakarta. Total protein pollens of palm trees, coconut, pine, acacia, grass, maize, and rice were analyzed in denatured-state by SDS-PAGE and are dominated by proteins with molecular weight of 10-70 kD. Skin prick test with allergen protein of pollen were tested to sixty nine subjects whom have been diagnosed with respiratory allergic and sixty nine subjects without history of allergy. The results among respiratory allergic patients, showed that pollen from grass and acacia demonstrated a higher percentage of sensitivity response compared with other pollen.Keywords: pollen, protein, sensitivity, skin prick test, allergen
Potret Kebijakan Pengendalian Demam Berdarah Dengue di Kabupaten Indramayu Peranginangin, Henri; -, Hasim; Pramudya, Bambang; Budiarti, Sri
Kesmas The National Journal of Public Health Vol. 4 No. 3 December 2009
Publisher : Kesmas The National Journal of Public Health

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Abstract

Dengue haemorrhagic fever (DHF) still becomes health problem in Indramayu district. The objective of this research is to identify the priority elements of DHF controlling policy in Indramayu district according to 35 experts using analytical hierarchy process (AHP). The result of the research provides information that the “main strategy” of DHF controlling is the improvement of healthy living environment; the “main actor” is the Government of Indramayu district; the “main factor” is the environment; the “main objective” is zero DHF in Indramayu district; and the “main criteria” is the quantity and quality of human resources. Based on this data, in order to implement the main strategy effectively, the Government of Indramayu district should increase the inter-program and inter-institutional cooperation; provide technological, funding, and facilities of environment health education supports; increase the quality of Puskesmas services and develop the DHF controlling team in all administrative level.Key words: Controlling, DHF, AHP, health, environment
Analysis of Rumen Microbial Population of Cattle Given Silage and Probiotics Using Terminal Restriction Fragment Length Polymorphism RIDWAN1, RONI; WIDYASTUTI1, YANTYATI; BUDIARTI, SRI; DINOTO, ACHMAD
Microbiology Indonesia Vol 3, No 3 (2009): December 2009
Publisher : Indonesian Society for microbiology

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Abstract

Rumen ecology is an important observation in evaluating the effectivity of silage and probiotic additives relating to their roles in cattle productivity. The objective of this study was to examine the effects of silage and probiotics on ruminal ecosystems in vivo using a molecular approach. Terminal-restriction fragment-length-polymorphism (T-RFLP) analysis was used to detect changes of ecological communities based on 16S-ribosomal deoxyribonucleic acid (16S-rDNA). Two rumen canulated PO cattle were fed several diets i.e.; (R0) basal diet dry matter basis (Pennisetum purpureum 70% and commercial concentrate 30%), (R1) silage (basal diet fermented using Lactobacillus plantarum BTCC570), (R2) silage + probiotics (L. plantarium Str BTCC531), (R3) Basal diet + probiotics (L. plantarium Str BTCC531). Digesta samples were collected 3 h after feeding for pH and T-RFLP analysis. T-RFLP analysis was performed using the 16S-rDNA amplified from each sample. The lengths of the terminal restriction fragments were analysed after digestion with HhaI, HaeIII and MspI. Results showed the effectivenes of silage and probiotics, given together, on the index of Smith and Wilson evenness applied to T-RFLP ecology data (Evar) with 0.89±0.04 being the highest. The diversity of rumen microorganisms is influenced by individual differences of each animal. T-RFLP analysis has a potency to be used for comparisons of complex bacterial communities, especially to detect changes in community structure in response to different variables and to show rumen bacteria diversity in the rumen.
Pemurnian Ekstraseluler Hyaluronidase Streptococcus agalactiae (Streptokokus Grup B ) (Extracellular Hyaluronidase Purification of Streptococcus agalactiae (Group B of Streptococus) Putranto, Wendry Setiyadi; Budiarti, Sri; Suhartono, Maggy T.; Wibawan, I Wayan T.; Hayati, Zainatul
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 6, No 1 (2006)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v6i1.2260

Abstract

Hyaluronidase (EC 4.2.2.1) merupakan enzim ekstraseluler yang dihasilkan Streptococcus agalactiae (Streptokokus Grup B). Penelitian ini bertujuan untuk mengetahui aktivitas spesifik dari hyaluronidase S. agalactiae dan berat molekul proteinnya. Pemurnian enzim dengan sentrifugasi, pengendapan amonium sulfat 45% dan kromatographi kolom dengan Sephadex G-100 dan Sodiumdodecyl sulfate polyacrylainide gel electrophoresis (SDS PAGE). Enzim hyaluronidase dari S. Agalactiae memiliki aktivitas spesifik sebesar 0,0012 U/mg (ekstrak kasar), 0,0125 U/mg (pengendapan dengan 45% ammonium sulfate) and 0,032 U/mg (Gel Filtration). Berat molekul protein hyaluronidase adalah 102 - 106 kD.Kata kunci : hyaluronidase, aktivitas spesifik, pemurnian
Potret Kebijakan Pengendalian Demam Berdarah Dengue di Kabupaten Indramayu Peranginangin, Henri; Hasim, Hasim; Pramudya, Bambang; Budiarti, Sri
Jurnal Kesehatan Masyarakat Nasional Vol. 4 No. 3 Desember 2009
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21109/kesmas.v4i3.185

Abstract

Penyakit demam berdarah dengue (DBD) masih menjadi masalah di Kabupaten Indramayu. Penelitian ini bertujuan mengidentifikasi elemen-elemen priori-tas kebijakan pengendalian penyakit DBD di Kabupaten Indramayu menurut pakar berdasarkan metode Analytical Hierarchy Process (AHP). Hasil peneliti-an menunjukkan “strategi utama” pengendalian penyakit DBD di Kabupaten Indramayu adalah peningkatan kesehatan lingkungan permukiman; “aktor utama pengendalian” ialah Pemerintah Kabupaten Indramayu; “faktor utama pengendalian” adalah lingkungan; “tujuan utama pengendalian” ialah Kabupaten Indramayu bebas penyakit DBD; dan “kriteria utama pengendalian” adalah jumlah dan mutu sumber daya manusia. Agar implementasi strategi pengendali-an itu efektif maka Pemerintah Kabupaten Indramayu perlu meningkatkan kerja sama lintas program dan sektoral; dukungan teknologi, dana dan sarana pendidikan kesehatan lingkungan; mutu layanan Puskesmas; dan pengembangan tim pengendalian penyakit DBD dari tingkat Kabupaten sampai Desa/Kelurahan.Kata kunci : Pengendalian, DBD, AHP, kesehatan, lingkungan.AbstractDengue haemorrhagic fever (DHF) still becomes health problem in Indramayu district. The objective of this research is to identify the priority elements of DHF controlling policy in Indramayu district according to 35 experts using analytical hierarchy process (AHP). The result of the research provides information that the “main strategy” of DHF controlling is the improvement of healthy living environment; the “main actor” is the Government of Indramayu district; the “main factor” is the environment; the “main objective” is zero DHF in Indramayu district; and the “main criteria” is the quantity and quality of human resources. Based on this data, in order to implement the main strategy effectively, the Government of Indramayu district should increase the interprogram and inter-institu-tional cooperation; provide technological, funding, and facilities of environment health education supports; increase the quality of Puskesmas services and develop the DHF controlling team in all administrative level. Key words : Controlling, DHF, AHP, health, environment.
Salmonella population in waste water treatment installation (IPAK) at Pulo Gebang DKI Jakarta Budiarti, Sri; Rusmana, Iman
Medical Journal of Indonesia Vol 7 (1998): Supplement 1
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.v7iSupp1.1124

Abstract

[no abstract available]