Nyoman Sri Budayanti
Bagian Mikrobiologi Fakultas Kedokteran Universitas Udayana / Rumah Sakit Umum Pusat Sanglah Denpasar Bal

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PERBEDAAN GAMBARAN HISTOPATOLOGI GRANULOMA PARU MENCIT SETELAH DIINFEKSI MYCOBACTERIUM TUBERCULOSIS DAN ATAU INTERVENSI SILIKA (THE INFLUENCES OF TIME IN THE HISTOPATHOLOGY OF LUNG GRANULOMA IN MICE AFTER INFECTION OF MYCOBACTERIUM TUBERCULOSIS AND SILI Linawati, Ni Made; Mayun, I Gusti Ngurah; Wiryawan, I Gusti Nyoman Sri; Budayanti, Nyoman Sri; Mertaniasih, Ni Made; Ratam, Fedik Abdul; Wande, I Nyoman; Ratnayanti, I Gusti Ayu Dewi; Wahyuniari, Ida Ayu Ika; Sugiritama, I Wayan; Arijana, I Gusti Kamasan
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The characteristics of lung tuberculosis is granuloma, which is consisted of lymphocytes andmacrophages that show the interaction between immune cells and M.tb. Granuloma is the organizationprocess which is depend on lymphocytes invasion, adhesion molecules and chemokine fasilitation. Silicosiswhich is caused by silica, can influence granuloma in the lung. The features of granuloma is variationdepend on the elicited agent and immune reaction. The main purpose of this study was to prove thehistopathology differences of  mice lung granuloma caused by M.tb infection,  silica intervention and bothin 3th  and 7th weeks. It was 45 mice Balb-c strain, divided into 3 groups;  P1 got  M.tb infection with H37Rvstrain 105  perml,P2 got silica intervention with 60 micro litre and, P3 got both of M.tb infection and  silica intervention. Termination of each group were held on 3 and 7 weeks of intervention, continued byhistopathology examination. In the histopathology feature, we done semi-quantitative prosedure to measurelung damage by using Dormans scores; perivasculitis, peribronchiolitis, alveolitis and granuloma. Oneway anova to analysis the differences of histopathologycal result among these groups (P< 0,05).  Resultshowed the significant differences  among these group.  In the 3th weeks, we found  mild lung damage werehappened in all groups with granuloma, without necrosic (P1 and P2). In the 7th weeks we found  severe lungdamage in P3 with necrotic and fibrotic granuloma sign, with necrosis in P1, with fibrotic in P2.  Weconcluded the worst lung damage happened in 7th weeks in group which are got M.tb infection and silicaintervention, with granuloma characterictic of necrosic and fibrotic.
CRYOTHERAPY PADA DESCEMETOCELE REKUREN POST-PERIOSTEAL GRAFT : LAPORAN KASUS PADA KERATOKONJUNGTIVITIS GONOKOKUS Yenny K, Nyoman; Susila, NK Niti; Budayanti, Nyoman Sri
Medicina Vol 46 No 3 (2015): September 2015
Publisher : Medicina

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Abstract

Keratokonjungtivitis gonokokus disebabkan oleh Neisseria gonorrhoeae, kokus gram negatif yangmenginvasi epitel kornea intak menyebabkan keratolisis stroma kornea hingga perforasi. Membrandescemet merupakan barrier terhadap mikroorganisme, namun ketika ditembus oleh mikroorganisme,akan  menonjol ke depan membentuk descemetocele. Dilaporkan satu kasus descemetocele padakeratokonjungtivitis gonokokus pada wanita usia 45 tahun di Rumah Sakit Umum Pusat SanglahDenpasar. Penderita mengeluh pandangan buram, kotoran mata kekuningan, kental sejak 6 hari,kelopak mata bengkak dan bintik hitam di mata kanan. Tajam penglihatan mata kanan 5/60.Pemeriksaan oftalmologi pada mata kanan didapat udem palpebra , konjungtiva sekret mukopurulenmasif, conjunctival vascular injection dan pericorneal vascular injection, udemkornea dan descemetoceledi jam 1, ukuran  3x2 mm, bilik mata depan dangkal dan fibrin, iris retraksi, pupil ireguler dan reflekpupil negatif, lensa tertutup fibrin, vitreus sulit dievaluasi, reflek fundus positif. Pada penderita telahdilakukan periosteal graft namun flap bergeser sehingga dilakukan tindakan ulangan cryotherapy yangmemberikan hasil baik. [MEDICINA 2015;46:184-8]Gonococcus keratoconjunctivitis caused by Neisseria gonorrhoeae, a gram-negative coccus thatinvading intact corneal epithel causing keratolysis until perforation. Descemet membrane is a barrierfor microorganism, but when penetrated by microorganism, it will bulging forming descemetocele.Wereported a case descemetoceleon gonococcus keratoconjunctivitis of a 45 years old woman in SanglahHospital-Denpasar.Patient complained blurry vision, white-yellowish secrete, purulent since 6 days,palpebra edema, and black dot on right eye. Right eye visual acuity was 5/60. Ophthalmologyexamination of palpebra was edema, massive conjunctival mucopurulene secrete, conjunctival vascularinjection, pericorneal vascular injection, corneal edema, and descemetocele at 1o?clock hour, size 3x2mm, shallow anterior chamber and fibrin, iris retracted, irregular pupil, negative pupil reflex, fibrincovered by lens, can not evaluated vitreous, funduscopy reflex positive.Patient had been treated withgraft periosteal but then the flap was sliding, so we treated patient with cryotherapy and gave goodresult. [MEDICINA 2015;46:184-8].
Udayana One Health Collaborating Center (OHCC) initiated Bali’s first mass, integrated basic life support training Sudewi, Anak Agung Raka; Budayanti, Nyoman Sri; Wiryana, Made; Senapathi, Tjokorda Gde Agung; Ryalino, Christopher; Pradhana, Adinda Putra
Bali Journal of Anesthesiology Vol 3, No 1 (2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.494 KB) | DOI: 10.15562/bjoa.v3i1.136

Abstract

ABSTRACTBasic Life Support (BLS) is a first-aid training that can be utilized in case of emergency until the victims are retained by medical professionals. Mastering BLS skills can be the difference between life and death. A cardiopulmonary resuscitation (CPR) performed by a passer-by is important to ensure a successful resuscitation in out-of-hospital cardiac arrest. Yet, improving the survival rate for out-of-hospital cardiac arrest is still a major problem. Starting in 2019, Udayana One Health Collaborating Center (Udayana OHCC) will implement the first mass and integrated BLS training in Bali. The goals are to introduce BLS to more people and to produce BLS-friendly environment in Bali.