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The Manipulation effectivity of cell co-cultures in 5% CO2 incubation system to increase in vitro cattle embryo production Syaiful, Ferry Lismanto; BP, Zesfin; Saladin, R; ., Jaswandi; ., Hendri
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v15i1.678

Abstract

The purpose of this research is to determine the effectivity of various cell co-cultures of cattle embryo production by in vitro CO2 5% incubation system and get the best cell co-culture. Cell co-culture which are used in the synthesis is the oviduct cells, isthmus cells, ampulla cells, follicle cells and without cells. Data were analyzed based on completely randomized desiggn. The average growth rate/ cleavage in various cell culture was: the oviduct cell 59.24%, ampulla cell  58.69%, isthmus cell 58.25%, follicle cell 52.24% and without cells 47.76%. The average  growth of 8-16 cells embryos to various cell co-culture was: the oviduct cell 46.02%, ampulla cell 45.45%, isthmus cell 45.15%, follicle cell 43.07%, and without cell 38.50%. The mean percentage of morula in various cell co-culture treatment was: the oviduct cell 20.59%, ampulla cell 20.48%, isthmus cell 20.30%, follicle cell 16.96% and without cell 12.58%. The average percentage of embryonic growth (cleavage, 8-16 cells and morula) was not significantly different (P > 0.05).  The treatment of a variety of cell co-culture increased significantly (P>0.05), blastocysts production, namely: the oviduct cell 3.28%, ampulla cell 3.22%, isthmus cell 3.08%, follicle cell 2.45% and without cell 1.97%.  In conclusion, the treatment of various cell co-culture in 5%CO2   incubation system can increace the growth of cattle embryos in vitro.   Key words: Cell Co-Culture, In Vitro Embryo, 5%CO2 Incubation System, Cattle
The Manipulation effectivity of cell co-cultures in 5% CO2 incubation system to increase in vitro cattle embryo production Syaiful, Ferry Lismanto; BP, Zesfin; Saladin, R; ., Jaswandi; ., Hendri
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v15i1.678

Abstract

The purpose of this research is to determine the effectivity of various cell co-cultures of cattle embryo production by in vitro CO2 5% incubation system and get the best cell co-culture. Cell co-culture which are used in the synthesis is the oviduct cells, isthmus cells, ampulla cells, follicle cells and without cells. Data were analyzed based on completely randomized desiggn. The average growth rate/ cleavage in various cell culture was: the oviduct cell 59.24%, ampulla cell  58.69%, isthmus cell 58.25%, follicle cell 52.24% and without cells 47.76%. The average  growth of 8-16 cells embryos to various cell co-culture was: the oviduct cell 46.02%, ampulla cell 45.45%, isthmus cell 45.15%, follicle cell 43.07%, and without cell 38.50%. The mean percentage of morula in various cell co-culture treatment was: the oviduct cell 20.59%, ampulla cell 20.48%, isthmus cell 20.30%, follicle cell 16.96% and without cell 12.58%. The average percentage of embryonic growth (cleavage, 8-16 cells and morula) was not significantly different (P > 0.05).  The treatment of a variety of cell co-culture increased significantly (P>0.05), blastocysts production, namely: the oviduct cell 3.28%, ampulla cell 3.22%, isthmus cell 3.08%, follicle cell 2.45% and without cell 1.97%.  In conclusion, the treatment of various cell co-culture in 5%CO2   incubation system can increace the growth of cattle embryos in vitro.   Key words: Cell Co-Culture, In Vitro Embryo, 5%CO2 Incubation System, Cattle
Pengaruh Pemberian Kromium (III) Klorida Terhadap Kadar SGPT Darah Mencit Putih Dharma, Surya; BP, Zesfin; H, Irsan Ryanto; Zubir, Nasrul
Jurnal Sains dan Teknologi Farmasi Vol 15 No 1 (2010)
Publisher : Fakultas Farmasi Universitas Andalas

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Abstract

The researh has been done on the influence of giving chromium (III) chloride on white mice blood SGPT.  The researh was divided into 5 groups, that is control,  doses ( 5,2; 6,37 and 7,8 ug/20 BW) with vitamin C and the dose of 7,8 ug/20 g BW without vitamin C. The observation was done for 42 days, that is day 7 th, 21 st and 42 nd with parameter SGPT concentration level. Results indicated that all doses showed the increase in SGPT concentration significantly (p<0,05).
PENGARUH PEMBERIAN KROMIUM (III) KLORIDA TERHADAP SPERMATOZOA MENCIT PUTIH Dharma, Surya; BP, Zesfin; Zubir, Nasrul; H, Irsan Ryanto.
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 5, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

A study on the effect of chromium chloride on motility, viability and totality of spermatozoa the male with mice. The drugs was given  orally with doses 5,2 ; 6,37 and 7,8 µg/20 g BB with vitamin C and doses  7,8 µg/20 g BB without vitamin C. The treatment and giving of drugs were carried out  for 40 days and removal of spermatozoa was done at at the day 7, 21 and 42 were taken vas deferens and epididymis, until result the suspension of spermatozoa. The result indicated that the giving chromium (III) chloride with vitamin C give effect to decrease motility, viability and totality of spermatozoa the male wihite mice at p<0,05 ABSTRAK Telah dilakukan penelitian tentang pengaruh pemberian senyawa kromium (III) klorida terhadap motilitas, viabilitas dan jumlah spermatozoa mencit putih jantan. Hewan dibagi atas 5 kelompok  dengan 3 variasi dosis ( 5,2; 6,37 dan 7,8 µg/20 BB) dengan lama perlakuan selama 42 hari yang diamati pada hari ke 7, 21 dan 42.. Hasil menunjukan bahwa setelah pemberian senyawa kromium (III) klorida pada 3 variasi dosis terjadi penurunan motilitas, viabilitas dan jumlah spermatozoa mencit putih jantan secara bermakna
The Manipulation effectivity of cell co-cultures in 5% CO2 incubation system to increase in vitro cattle embryo production Syaiful, Ferry Lismanto; BP, Zesfin; Saladin, R; ., Jaswandi; ., Hendri
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v15i1.678

Abstract

The purpose of this research is to determine the effectivity of various cell co-cultures of cattle embryo production by in vitro CO2 5% incubation system and get the best cell co-culture. Cell co-culture which are used in the synthesis is the oviduct cells, isthmus cells, ampulla cells, follicle cells and without cells. Data were analyzed based on completely randomized desiggn. The average growth rate/ cleavage in various cell culture was: the oviduct cell 59.24%, ampulla cell  58.69%, isthmus cell 58.25%, follicle cell 52.24% and without cells 47.76%. The average  growth of 8-16 cells embryos to various cell co-culture was: the oviduct cell 46.02%, ampulla cell 45.45%, isthmus cell 45.15%, follicle cell 43.07%, and without cell 38.50%. The mean percentage of morula in various cell co-culture treatment was: the oviduct cell 20.59%, ampulla cell 20.48%, isthmus cell 20.30%, follicle cell 16.96% and without cell 12.58%. The average percentage of embryonic growth (cleavage, 8-16 cells and morula) was not significantly different (P > 0.05).  The treatment of a variety of cell co-culture increased significantly (P>0.05), blastocysts production, namely: the oviduct cell 3.28%, ampulla cell 3.22%, isthmus cell 3.08%, follicle cell 2.45% and without cell 1.97%.  In conclusion, the treatment of various cell co-culture in 5%CO2   incubation system can increace the growth of cattle embryos in vitro.   Key words: Cell Co-Culture, In Vitro Embryo, 5%CO2 Incubation System, Cattle
PENGARUH PEMBERIAN KROMIUM (III) KLORIDA TERHADAP SPERMATOZOA MENCIT PUTIH Dharma, Surya; BP, Zesfin; Zubir, Nasrul; H, Irsan Ryanto.
Jurnal Farmasi Indonesia Vol 5, No 2 (2010)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v5i2.43

Abstract

A study on the effect of chromium chloride on motility, viability and totality of spermatozoa the male with mice. The drugs was given  orally with doses 5,2 ; 6,37 and 7,8 µg/20 g BB with vitamin C and doses  7,8 µg/20 g BB without vitamin C. The treatment and giving of drugs were carried out  for 40 days and removal of spermatozoa was done at at the day 7, 21 and 42 were taken vas deferens and epididymis, until result the suspension of spermatozoa. The result indicated that the giving chromium (III) chloride with vitamin C give effect to decrease motility, viability and totality of spermatozoa the male wihite mice at p<0,05 ABSTRAK Telah dilakukan penelitian tentang pengaruh pemberian senyawa kromium (III) klorida terhadap motilitas, viabilitas dan jumlah spermatozoa mencit putih jantan. Hewan dibagi atas 5 kelompok  dengan 3 variasi dosis ( 5,2; 6,37 dan 7,8 µg/20 BB) dengan lama perlakuan selama 42 hari yang diamati pada hari ke 7, 21 dan 42.. Hasil menunjukan bahwa setelah pemberian senyawa kromium (III) klorida pada 3 variasi dosis terjadi penurunan motilitas, viabilitas dan jumlah spermatozoa mencit putih jantan secara bermakna